scholarly journals Control of Epinotia granitalis(Lepidoptera:Tortricidae)with Paecilomyces spp. on Cotton Bands Wrapped on the Trunks of Cryptomeria japonica

1992 ◽  
Vol 27 (2) ◽  
pp. 295-296 ◽  
Author(s):  
Wataru MITSUHASHI ◽  
Mitsuaki SHIMAZU ◽  
Hoshimi HASHIMOTO
Keyword(s):  
Cryptomeria Japonica ◽  
Epinotia Granitalis

A New Seco-Abietanoid from the Bark of Cryptomeria japonica

2021 ◽  
Author(s):  
Chi-I Chang ◽  
Cheng-Chi Chen ◽  
Yueh-Hsiung Kuo
Keyword(s):  
Cryptomeria Japonica

scholarly journals CRISPR/Cas9-mediated targeted mutagenesis in Japanese cedar (Cryptomeria japonica D. Don)

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yoshihiko Nanasato ◽  
Masafumi Mikami ◽  
Norihiro Futamura ◽  
Masaki Endo ◽  
Mitsuru Nishiguchi ◽  
...  
Keyword(s):  
Genome Editing ◽  
Cryptomeria Japonica ◽  
Fluorescent Protein ◽  
Japanese Cedar ◽  
Targeted Mutagenesis ◽  
Embryogenic Tissue ◽  
Breeding Period ◽  
Single Mutation ◽  
Knock Out ◽  
Guide Rna

AbstractCryptomeria japonica (Japanese cedar or sugi) is one of the most important coniferous tree species in Japan and breeding programs for this species have been launched since 1950s. Genome editing technology can be used to shorten the breeding period. In this study, we performed targeted mutagenesis using the CRISPR/Cas9 system in C. japonica. First, the CRISPR/Cas9 system was tested using green fluorescent protein (GFP)-expressing transgenic embryogenic tissue lines. Knock-out efficiency of GFP ranged from 3.1 to 41.4% depending on U6 promoters and target sequences. The GFP knock-out region was mottled in many lines, indicating genome editing in individual cells. However, in 101 of 102 mutated individuals (> 99%) from 6 GFP knock-out lines, embryos had a single mutation pattern. Next, we knocked out the endogenous C. japonica magnesium chelatase subunit I (CjChlI) gene using two guide RNA targets. Green, pale green, and albino phenotypes were obtained in the gene-edited cell lines. Sequence analysis revealed random deletions, insertions, and replacements in the target region. Thus, targeted mutagenesis using the CRISPR/Cas9 system can be used to modify the C. japonica genome.

scholarly journals Anti-allergic Substances Contained in the Pollen of Cryptomeria japonica Possess Diverse Effects on the Degranulation of RBL-2H3 Cells

2003 ◽  
Vol 92 (3) ◽  
pp. 291-295 ◽  
Author(s):  
Yukiko Morimoto ◽  
Tadashi Yasuhara ◽  
Akiko Sugimoto ◽  
Atsuko Inoue ◽  
Izumi Hide ◽  
...  
Keyword(s):  
Cryptomeria Japonica

Mating patterns in an indoor miniature Cryptomeria japonica seed orchard as revealed by microsatellite markers

New Forests ◽  
2009 ◽  
Vol 39 (3) ◽  
pp. 261-273 ◽  
Author(s):  
Yoshinari Moriguchi ◽  
Yoko Yamazaki ◽  
Hideaki Taira ◽  
Yoshihiko Tsumura
Keyword(s):  
Microsatellite Markers ◽  
Cryptomeria Japonica ◽  
Seed Orchard ◽  
Mating Patterns
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