scholarly journals Infection of a Bombyx Cell Line with a Cytoplasmic Polyhedrosis Virus of the White Cutworm, Euxoa scandens(Lepidoptera : Noctuidae)

1989 ◽  
Vol 24 (3) ◽  
pp. 318-320 ◽  
Author(s):  
Hajime INOUE ◽  
Serge BELLONCIK
Keyword(s):  
Cell Line ◽  
Cytoplasmic Polyhedrosis Virus ◽  
Polyhedrosis Virus ◽  
Lepidoptera Noctuidae

INFLUENCE OF INSECTICIDES - CYTOPLASMIC POLYHEDROSIS VIRUS COMBINATIONS ON PUPAL WEIGHT AND FECUNDITY OF TOBACCO BUDWORM (LEPIDOPTERA: NOCTUIDAE)

1989 ◽  
Vol 24 (4) ◽  
pp. 539-544 ◽  
Author(s):  
Abdul K. Mohamed ◽  
Jen-Rong Yang ◽  
Fred R. S. Nelson
Keyword(s):  
Heliothis Virescens ◽  
Methyl Parathion ◽  
Adult Emergence ◽  
Tobacco Budworm ◽  
Pupal Weight ◽  
Egg Hatch ◽  
Cytoplasmic Polyhedrosis Virus ◽  
Polyhedrosis Virus ◽  
Control Adult ◽  
Lepidoptera Noctuidae

Laboratory studies were conducted to determine the response of healthy and cytoplasmic polyhedrosis virus (CPV) infected Heliothis virescens (F.) larvae to LD50 of methomyl and methyl parathion. Methomyl-CPV combination adversely affected pupal weight and fecundity of the female. The effect of this interaction was synergistic. Exposure of CPV infected larvae to LD50 of methomyl resulted in a mean female pupal weight of 243 mg and a fecundity of 382 per female moth. These values were significantly lower than those of all other treatments. In contrast methyl parathion-CPV combinations did not result in a significant reduction in pupal weight as compared to the control. Adult emergence and egg hatch were not affected.

Histopathology of cytoplasmic polyhedrosis virus (Reoviridae) infection in corn earworm, Helicoverpa zea (Boddie), larvae (Insecta: Lepidoptera: Noctuidae)

1991 ◽  
Vol 69 (8) ◽  
pp. 2121-2127 ◽  
Author(s):  
C. F. J. Bong ◽  
P. P. Sikorowski
Keyword(s):  
Helicoverpa Zea ◽  
Heliothis Zea ◽  
Goblet Cells ◽  
Corn Earworm ◽  
Cell Organelles ◽  
Cytoplasmic Polyhedrosis Virus ◽  
Polyhedrosis Virus ◽  
Columnar Cells ◽  
Lepidoptera Noctuidae ◽  
Virogenic Stroma

Histopathology of cytoplasmic polyhedrosis virus (CPV) infection in the larval midgut of the corn earworm, Helicoverpa (=Heliothis) zea (Boddie) is described. Small polyhedral inclusion bodies (PIB) are observed in columnar cells of the midgut 1 or 2 days after treatment with CPV. Virions are partially or completely occluded in a polyhedral matrix to form PIB at the periphery of the virogenic stroma. PIB are dodecahedral in shape with a hexagonal outline and measure from less than 1 to 4 μm in size. Virions measure about 50 nm in diameter. Microvilli of infected columnar cells are not affected until immediately prior to rupture of the cell. Some infected columnar cells ruptured to release PIB into the gut lumen 2 or 3 days after infection. PIB are also released into the lumen by extrusion of heavily infected columnar cells. Mitochondria and rough endoplasmic reticulum deteriorate as infection progresses, and in many cells with advanced infection, the nucleus is obscured. PIB are found in goblet cells 5 days after infection. Infected goblet cells degenerate to such an extent that only a few of the original microvillus-like cytoplasmic projections and cell organelles are left. The implications of CPV infection in integrated pest management of the corn earworm are discussed.

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