scholarly journals Specific PCR assays for the detection of DNA from Beauveria bassiana F-263, a highly virulent strain affecting the Japanese pine sawyer, Monochamus alternatus (Coleoptera: Cerambycidae), by a sequence-characterized amplified region (SCAR) marker

2007 ◽  
Vol 42 (4) ◽  
pp. 619-628 ◽  
Author(s):  
Jun Takatsuka
Keyword(s):  
Beauveria Bassiana ◽  
Scar Marker ◽  
Virulent Strain ◽  
Monochamus Alternatus ◽  
Sequence Characterized Amplified Region ◽  
Specific Pcr ◽  
Pcr Assays ◽  
Japanese Pine Sawyer ◽  
Highly Virulent

scholarly journals A DNA-Based Procedure for In Planta Detection of Fusarium oxysporum f. sp. phaseoli

2002 ◽  
Vol 92 (3) ◽  
pp. 237-244 ◽  
Author(s):  
Fernando M. Alves-Santos ◽  
Brisa Ramos ◽  
M. Asunción García-Sánchez ◽  
Arturo P. Eslava ◽  
José María Díaz-Mínguez
Keyword(s):  
Common Bean ◽  
Fusarium Oxysporum ◽  
Scar Marker ◽  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Rapd Marker ◽  
In Planta ◽  
Sequence Characterized Amplified Region ◽  
Polymerase Chain ◽  
Highly Virulent

We have characterized strains of Fusarium oxysporum from common bean fields in Spain that were nonpathogenic on common bean, as well as F. oxysporum strains (F. oxysporum f. sp. phaseoli) pathogenic to common bean by random amplified polymorphic DNA (RAPD) analysis. We identified a RAPD marker (RAPD 4.12) specific for the highly virulent pathogenic strains of the seven races of F. oxysporum f. sp. phaseoli. Sequence analysis of RAPD 4.12 allowed the design of oligonucleotides that amplify a 609-bp sequence characterized amplified region (SCAR) marker (SCAR-B310A280). Under controlled environmental and greenhouse conditions, detection of the pathogen by polymerase chain reaction was 100% successful in root samples of infected but still symptomless plants and in stem samples of plants with disease severity of ≥4 in the Centro Internacional de Agricultura Tropical (CIAT; Cali, Colombia) scale. The diagnostic procedure can be completed in 5 h and allows the detection of all known races of the pathogen in plant samples at early stages of the disease with no visible symptoms.

Protective effect of Trypanosoma rangeli against infections with a highly virulent strain of Trypanosoma cruzi

1997 ◽  
Vol 2 (5) ◽  
pp. 482-487 ◽  
Author(s):  
Claudio Zuniga ◽  
Teresa Palau ◽  
Pilar Penin ◽  
Carlos Gamallo ◽  
Jose Antonio de Diego
Keyword(s):  
Trypanosoma Cruzi ◽  
Protective Effect ◽  
Virulent Strain ◽  
Trypanosoma Rangeli ◽  
Highly Virulent

scholarly journals CRISPR Is an Optimal Target for the Design of Specific PCR Assays for Salmonella enterica Serotypes Typhi and Paratyphi A

2014 ◽  
Vol 8 (1) ◽  
pp. e2671 ◽  
Author(s):  
Laetitia Fabre ◽  
Simon Le Hello ◽  
Chrystelle Roux ◽  
Sylvie Issenhuth-Jeanjean ◽  
François-Xavier Weill
Keyword(s):  
Salmonella Enterica ◽  
Specific Pcr ◽  
Optimal Target ◽  
Pcr Assays
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