scholarly journals Characterization of Beauveria bassiana isolates from Japan using inter-simple-sequence-repeat-anchored polymerase chain reaction (ISSR-PCR) amplification

2007 ◽  
Vol 42 (4) ◽  
pp. 563-571 ◽  
Author(s):  
Jun Takatsuka
Keyword(s):  
Simple Sequence Repeat ◽  
Pcr Amplification ◽  
Inter Simple Sequence Repeat ◽  
Sequence Repeat ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Beauveria Bassiana Isolates ◽  
Issr Pcr ◽  
Simple Sequence

MOLECULAR CHARACTERIZATION OF TRICHINELLA GENOTYPES BY INTER-SIMPLE SEQUENCE REPEAT POLYMERASE CHAIN REACTION (ISSR-PCR)

2006 ◽  
Vol 92 (3) ◽  
pp. 606-610 ◽  
Author(s):  
F. Fonseca-Salamanca ◽  
J. J. Nogal-Ruiz ◽  
C. Benito ◽  
M. V. Camacho ◽  
A. R. Martínez-Fernández
Keyword(s):  
Polymerase Chain Reaction ◽  
Molecular Characterization ◽  
Simple Sequence Repeat ◽  
Inter Simple Sequence Repeat ◽  
Sequence Repeat ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Issr Pcr ◽  
Simple Sequence

Identifi cation of Species-Diagnostic Inter Simple Sequence Repeat Markers for Ten Phyllanthus Species

2011 ◽  
Vol 66 (3-4) ◽  
pp. 167-172 ◽  
Author(s):  
Sunil Kumar Senapati ◽  
Subhashree Aparajita ◽  
Gyana Ranjan Rout
Keyword(s):  
Simple Sequence Repeat ◽  
Viral Infections ◽  
Pcr Amplification ◽  
Inter Simple Sequence Repeat ◽  
Diagnostic Markers ◽  
Sequence Repeat ◽  
Botanical Drug ◽  
Polymerase Chain ◽  
Medicinal Plant Material ◽  
Simple Sequence

Phyllanthus has been widely used in traditional medicine as an antipyretic, a diuretic, and to treat liver diseases and viral infections. Correct genotype identification of medicinal plant material remains important for the botanical drug industry. Limitations of chemical and morphological approaches for authentication have generated the need for newer methods in quality control of botanicals. In the present study, attempts were made to identify species- diagnostic markers for ten Phyllanthus species using the inter simple sequence repeat-polymerase chain reaction (ISSR-PCR) fingerprinting method. PCR amplification using seven ISSR primers resulted in significant polymorphism among the populations from different species. P. angustifolius and P. urinaria showed monomorphic frequency of maximum (63.88%) and minimum (20.64%), respectively. Seventeen species-diagnostic markers were identified for seven species (P. acidus, P. emblica, P. fraternus, P. urinaria, P. rotundifolius, P. amarus, and P. angustifolius) while no marker was detected for P. reticulatus, P. nivosus, and P. virgulatus. A maximum of six species-diagnostic markers were identified for P. acidus and a minimum of only one of 755 bp was available for P. amarus. Among the seventeen markers, nine were present in all individuals of particular species. The speciesspecific differences in fragment numbers and sizes could be used as diagnostic markers to distinguish the Phyllanthus species quickly

Optimization of Polymerase Chain Reaction for Inter Simple Sequence Repeat Technique for Four Species of Plants

2016 ◽  
Vol 683 ◽  
pp. 511-518 ◽  
Author(s):  
Polina Gudkova ◽  
Eugene Bayahmetov
Keyword(s):  
Polymerase Chain Reaction ◽  
Simple Sequence Repeat ◽  
Inter Simple Sequence Repeat ◽  
Pteridium Aquilinum ◽  
Minimal Amount ◽  
Sequence Repeat ◽  
Chain Reaction ◽  
Imaging Results ◽  
Polymerase Chain ◽  
Simple Sequence

Polymerase chain reaction optimization for inter simple sequence repeat primers is a key factor to obtain accurate and reproducible results for gene mapping, studying the genetic structure of populations, plant passporting, phylogenetic analysis. Changing temperature conditions, the amount of amplification cycles and concentration of reaction mixture components is allowed to vary the number of bands obtained by this method. This article is result of preliminary research of method selection for molecular analysis. It is aimed to show how to adjust the profile of inter simple sequence repeat fragments by polymerase chain reaction for four model species Stipa lessingiana, Poa intricata, Equisetum fluviatile and Pteridium aquilinum. The working concentrations of magnesium chloride for primer ((СТС)3GC) and ((АС)8YG) were 2.5 mM for 0.63 units of Taq DNA polymerase and for primer ((СА)6GG) it was 4.5 mM for 1.25 units. Sharply defined banding was observed from the minimal amount of DNA 5 ng per reaction, with primer concentration from 10 to 80 pmol and dNTPs concentration 0.2 mM. Optimal hybridization temperatures were 51.9 °C for primers ((АС)8YG), ((СА)6GG) and 50.0 °C for ((СТС)3GC). The best imaging results were obtained when setting up electrophoresis in 1.9% agarose gel

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