scholarly journals Discrimination of Japanese isolates of Beauveria brongniartii (Deuteromycotina: Hyphomycetes) by RFLP of the rDNA-ITS regions

2003 ◽  
Vol 38 (4) ◽  
pp. 551-557 ◽  
Author(s):  
Sanae Wada ◽  
Mitsuo Horita ◽  
Kazuyuki Hirayae ◽  
Mitsuaki Shimazu
Keyword(s):  
Beauveria Brongniartii ◽  
Rdna Its ◽  
Its Regions

Sequence variation of the rDNA ITS regions within and between anastomosis groups in Rhizoctonia solani

1997 ◽  
Vol 32 (3) ◽  
pp. 237-243 ◽  
Author(s):  
S. Kuninaga ◽  
Tomohide Natsuaki ◽  
Toru Takeuchi ◽  
Ryozo Yokosawa
Keyword(s):  
Rhizoctonia Solani ◽  
Sequence Variation ◽  
Anastomosis Groups ◽  
Rdna Its ◽  
Its Regions

Amplification of Fungal rDNA-Its Regions from Non-Fertile Specimens of the Lichen-Forming Genus Parmelia

1997 ◽  
Vol 29 (03) ◽  
pp. 275 ◽  
Author(s):  
Ana Crespo ◽  
Paul D. Bridge ◽  
David L. Hawksworth
Keyword(s):  
Rdna Its ◽  
Its Regions

scholarly journals Characterization of a New Anastomosis Group (AG-W) of Binucleate Rhizoctonia, Causal Agent for Potato Stem Canker

Plant Disease ◽  
2015 ◽  
Vol 99 (12) ◽  
pp. 1757-1763 ◽  
Author(s):  
Y. G. Yang ◽  
C. Zhao ◽  
Z. J. Guo ◽  
X. H. Wu
Keyword(s):  
Fragment Length Polymorphism ◽  
Stem Canker ◽  
Anastomosis Group ◽  
Northeastern China ◽  
Binucleate Rhizoctonia ◽  
Rdna Its ◽  
Its Regions ◽  
Pathogenicity Tests ◽  
Reference Strains

Two binucleate Rhizoctonia (BNR) isolates were recovered from potato cankered stems in Heilongjiang Province, northeastern China. Their cultural appearance on potato dextrose agar remained whitish as the cultures aged. White monilioid cells formed in the fluffy aerial hyphae, whereas no sclerotia appeared during the incubation. The two isolates could anastomose with each other, but they failed to anastomose with reference strains of BNR from AG-A to AG-Q, and AG-U. Analyses of restriction fragment length polymorphism (RFLP) of internal transcribed spacer of ribosomal DNA (rDNA-ITS) regions confirmed that these two isolates differed from the reference strains. The phylogenetic tree based on the sequences of rDNA-ITS regions showed that they were located in a distinct clade from other BNR AGs. These collective results suggested that the isolates recovered from potato in this study belonged to a new BNR AG designated as AG-W. Pathogenicity tests under glasshouse conditions revealed that both isolates were able to cause brown, dry, and slightly sunken lesions on potato subterranean stems. To our knowledge, this is the first report of the AG-W causing potato disease in China as well as worldwide.

Molecular Identification of Fitzroya Cupressoides, Sequoia Sempervirens, and Thuja Plicata Wood Using Taxon-Specific RDNA-ITS Primers

IAWA Journal ◽  
2011 ◽  
Vol 32 (2) ◽  
pp. 273-284 ◽  
Author(s):  
Felix Hanssen ◽  
Niko Wischnewski ◽  
Ute Moreth ◽  
Elisabeth A. Magel
Keyword(s):  
Sequence Divergence ◽  
Its Region ◽  
Sequoia Sempervirens ◽  
Nuclear Ribosomal Dna ◽  
Thuja Plicata ◽  
Length Variation ◽  
Rdna Its ◽  
Its Regions ◽  
Rdna Its Region ◽  
Fitzroya Cupressoides

The nuclear ribosomal DNA internal transcribed spacer (rDNA-ITS) region was PCR amplified and sequenced from the wood of three specimens of Fitzroya cupressoides, nine specimens of Sequoia sempervirens, and ten specimens of Thuja plicata. The full lengths of the ITS regions are 1110 bp for F. cupressoides, 1096 bp for S. sempervirens, and 1138 bp for T. plicata, and thus in the range of 975 bp to 1125 bp which is reported for members of the Cupressaceae. Length variation of ITS regions is due to differences in the length of the spacer region ITS1. Intraspecific variations of the sequences of rDNA-ITS regions were one bp in F. cupressoides, and 18 bp in S. sempervirens and T. plicata. Based on the interspecific sequence divergence of the ITS region, taxon-specific primers were designedfor the detection of F. cupressoides, S. sempervirens and T. plicata. The primer sequences were selected from the highly divergent ITS1 spacer. The specificity of the primers was checked by lengths and sequence of the amplicons, and the primers detected the target organism, solely, across 40 conifer species. Our data establish the molecular basis for DNA-based wood identification in these species.

Clavariadelphus elongatus sp. nov. (Basidiomycota; Clavariadelphaceae)-Addition to the Club Fungi of Pakistan

Phytotaxa ◽  
2018 ◽  
Vol 365 (2) ◽  
pp. 182 ◽  
Author(s):  
HASSAN SHER ◽  
JUNAID KHAN ◽  
ABDUL NASIR KHALID
Keyword(s):  
New Species ◽  
Molecular Data ◽  
Morphological Description ◽  
Nuclear Rdna ◽  
Anatomical Structures ◽  
Line Drawings ◽  
Rdna Its ◽  
Its Regions ◽  
A New Species ◽  
Reddish Orange

Clavariadelphus elongatus is described and illustrated as a new species from moist temperate forests of Pakistan. It is characterized by large subcylindrical basidiomata, light yellowish-pink to grayish-reddish orange coloration at maturity and ovoid to ellipsoid basidiospores. A morphological description and line drawings of anatomical structures for the new species are presented and comparison with closely allied taxa discussed. Molecular data inferred from nuclear rDNA ITS regions supports the recognition of the new species reported herein.

Sign in / Sign up

Export Citation Format

Share Document