scholarly journals Culture medium of Bacillus thuringiensis serovar japonensis Buibui enhances the insecticidal activities of .DELTA.-endotoxins from B. thuringiensis serovar kurstaki and aizawai against lepidopterous pest insects.

2000 ◽  
Vol 35 (4) ◽  
pp. 575-582 ◽  
Author(s):  
Shoji Asano ◽  
Katsutoshi Ogiwara ◽  
Makoto Takahashi ◽  
Leslie S. Indrasith
Keyword(s):  
Bacillus Thuringiensis ◽  
Culture Medium ◽  
Lepidopterous Pest ◽  
Pest Insects ◽  
Insecticidal Activities

scholarly journals The Correlation of the Presence and Expression Levels of cry Genes with the Insecticidal Activities against Plutella xylostella for Bacillus thuringiensis Strains

Toxins ◽  
2014 ◽  
Vol 6 (8) ◽  
pp. 2453-2470 ◽  
Author(s):  
Ming-Lun Chen ◽  
Pin-Hsin Chen ◽  
Jen-Chieh Pang ◽  
Chia-Wei Lin ◽  
Chin-Fa Hwang ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Plutella Xylostella ◽  
Cry Genes ◽  
Expression Levels ◽  
Insecticidal Activities

Effect of strain and medium variation on mosquito toxin production by Bacillus thuringiensis var. israelensis

1982 ◽  
Vol 28 (9) ◽  
pp. 1089-1092 ◽  
Author(s):  
Robert A. Smith
Keyword(s):  
Bacillus Thuringiensis ◽  
Growth Medium ◽  
Shake Flask ◽  
Culture Medium ◽  
Toxin Production ◽  
Shake Flask Culture ◽  
Mosquito Larvae ◽  
Flask Culture ◽  
Strain Variation ◽  
Spore Count

The effect of strain variation and culture medium on production of toxin lethal to mosquito larvae by Bacillus thuringiensis var. israelensis serotype H-14 was investigated. Shake flask culture of B. thuringiensis H-14 strains showed varied ability to produce toxins lethal to mosquito larvae dependent upon the particular strain and growth medium used. Buffered media demonstrated no better mosquito toxicity than did unbuffered media that ranged in pH from 5.7 to 8.1 at harvest. Although toxin production is associated with sporulation, spore count was generally not proportional to toxin produced for those strains and media evaluated.

COMPARAISON DE TOXICITÉ DE QUELQUES ÉLÉMENTS POUR BACILLUS THURINGIENSIS VAR. THURINGIENSIS BERL.

1967 ◽  
Vol 13 (1) ◽  
pp. 81-91 ◽  
Author(s):  
J. M. Perron ◽  
W. A. Smirnoff ◽  
L. Huot
Keyword(s):  
Cell Division ◽  
Bacillus Thuringiensis ◽  
Culture Medium ◽  
Toxic Effects ◽  
Clonal Structure ◽  
Structure Form ◽  
Cellular Lysis ◽  
Cobalt Copper

This study is concerned with the effects of aluminium, arsenic, boron, cobalt, copper, fluorine, iron, iodine, lithium, magnesium, molybdenum, and zinc on Bacillus thuringiensis var. thuringiensis Berliner. Addition of small quantities of the elements to the culture medium stimulated growth of the Bacillus. The effect was particularly marked during cell division, as revealed by an increase in area of the clones. The toxic effects of certain concentrations of the elements was also revealed during cell division, which, as indicated by the clonal area, was adversely affected by B, Cu, I, Mg, and Zn. F, Fe, Li, and Mo effected retardation of the cell division by as much as 12 to 48 hours. Al, As, Cu, and Mg both retarded and reduced cell division. By determining the relation between the effective limiting doses established, we were able to divide the elements into four groups. The toxicity of the elements was also revealed by its effects on the germination of the spores, clonal structure, form of the cells, time of sporulation, synthesis of the toxin-crystal, and cellular lysis.

Characterization of Flagellar Antigens and Insecticidal Activities of Bacillus thuringiensis Populations in Animal Feces

2003 ◽  
Vol 46 (4) ◽  
pp. 287-290 ◽  
Author(s):  
Dong-Hyun Lee ◽  
Noriko Shisa ◽  
Naoya Wasano ◽  
Akira Ohgushi ◽  
Michio Ohba
Keyword(s):  
Bacillus Thuringiensis ◽  
Animal Feces ◽  
Flagellar Antigens ◽  
Insecticidal Activities

A novel Bacillus thuringiensis strain and its pathogenicity against three important pest insects

2012 ◽  
Vol 51 ◽  
pp. 33-40 ◽  
Author(s):  
Semih Yılmaz ◽  
Abdurrahman Ayvaz ◽  
Mikail Akbulut ◽  
Ugur Azizoglu ◽  
Salih Karabörklü
Keyword(s):  
Bacillus Thuringiensis ◽  
Pest Insects ◽  
Important Pest ◽  
Thuringiensis Strain

scholarly journals Optimization of Cry3A Yields in Bacillus thuringiensis by Use of Sporulation-Dependent Promoters in Combination with the STAB-SD mRNA Sequence

1998 ◽  
Vol 64 (10) ◽  
pp. 3932-3938 ◽  
Author(s):  
Hyun-Woo Park ◽  
Baoxue Ge ◽  
Leah S. Bauer ◽  
Brian A. Federici
Keyword(s):  
Bacillus Thuringiensis ◽  
Type Strain ◽  
Culture Medium ◽  
Wild Type Strain ◽  
Expression System ◽  
Leaf Beetle ◽  
Wild Type ◽  
Additional Method ◽  
Chrysomela Scripta ◽  
Cottonwood Leaf Beetle

ABSTRACT The insecticidal activity of Bacillus thuringiensisstrains toxic to coleopterous insects is due to Cry3 proteins assembled into small rectangular crystals. Toxin synthesis in these strains is dependent primarily upon a promoter that is active in the stationary phase and a STAB-SD sequence that stabilizes the cry3transcript-ribosome complex. Here we show that significantly higher yields of Cry3A can be obtained by using dual sporulation-dependentcyt1Aa promoters to drive the expression ofcry3Aa when the STAB-SD sequence is included in the construct. The Cry3A yield per unit of culture medium obtained with this expression system was 12.7-fold greater than that produced by DSM 2803, the wild-type strain of B. thuringiensis from which Cry3Aa was originally described, and 1.4-fold greater than that produced by NB176, a mutant of the same strain containing two or three copies ofcry3Aa, which is the active ingredient of the commercial product Novodor, used for control of beetle pests. The toxicities of Cry3A produced with this construct or the wild-type strain were similar when assayed against larvae of the cottonwood leaf beetle,Chrysomela scripta. The volume of Cry3A crystals produced with cyt1Aa promoters and the STAB-SD sequence was 1.3-fold that of typical bipyramidal Cry1 crystals toxic to lepidopterous insects. The dual-promoter/STAB-SD system offers an additional method for potentially improving the efficacy of insecticides based onB. thuringiensis.

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