scholarly journals Cotoneaster Acuminatus Leaf Extract Mediated Synthesis, Characterization and in Vitro Evaluation of Antimicrobial Activity of Zinc Oxide Nanoparticles

2020 ◽  
Vol 36 (6) ◽  
pp. 1043-1048
Author(s):  
Goutam Kumar ◽  
P.P. Badoni ◽  
Arun K. Khajuria ◽  
Mahender Singh ◽  
Sapna Tyagi ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Zno Nanoparticles ◽  
Leaf Extract ◽  
Metal Oxide Nanoparticles ◽  
Research Work ◽  
Oxide Nanoparticles ◽  
Spectroscopic Techniques ◽  
Published Evidence ◽  
Aqueous Leaf Extract

In recent years, there is significant increase in the attention towards the green synthesis of metal oxide nanoparticles, particularly by the interaction of plant extracts and metal salts; still there is no such published evidence is available on the synthesis of ZnO nanoparticles from aqueous leaf extract of Cotoneaster acuminatus. The synthesis of ZnO nanoparticles was confirmed by using several spectroscopic techniques i.e., UV-Vis, FT-IR, powder-XRD and TEM. It was confirmed from the spectroscopic data that the synthesized nanoparticles were hexagonally orientated, size in the 16-38 nm range and encapsulated by biochemicals present in the leaf extract. These nanoparticles were further evaluated for antimicrobial activity Bacillus subtilisNCFT.583.08, Staphylococcus aureusNCFT.576.08, Pseudomonas aeruginosaNCFT.645.11, Candida albicansNCFT.1006.11 and Saccharomyces cerevisiae NCFT.1008.11strains. This research work might be considered as a successful attempt to create and evaluate medicinal properties of ZnO nanoparticles in combination with Cotoneaster acuminatus leaf extract.

scholarly journals Plant-Based Synthesis of Zinc Oxide Nanoparticles (ZnO-NPs) Using Aqueous Leaf Extract of Aquilegia pubiflora: Their Antiproliferative Activity against HepG2 Cells Inducing Reactive Oxygen Species and Other In Vitro Properties

2021 ◽  
Vol 2021 ◽  
pp. 1-14
Author(s):  
Hasnain Jan ◽  
Muzamil Shah ◽  
Anisa Andleeb ◽  
Shah Faisal ◽  
Aishma Khattak ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Leaf Extract ◽  
Antioxidant Properties ◽  
Aqueous Dispersion ◽  
Inhibitory Response ◽  
Oxide Nanoparticles ◽  
Zno Nps ◽  
Aqueous Leaf Extract

The anti-cancer, anti-aging, anti-inflammatory, antioxidant, and anti-diabetic effects of zinc oxide nanoparticles (ZnO-NPs) produced from aqueous leaf extract of Aquilegia pubiflora were evaluated in this study. Several methods were used to characterize ZnO-NPs, including SEM, FTIR, XRD, DLS, PL, Raman, and HPLC. The nanoparticles that had a size of 34.23 nm as well as a strong aqueous dispersion potential were highly pure, spherical or elliptical in form, and had a mean size of 34.23 nm. According to FTIR and HPLC studies, the flavonoids and hydroxycinnamic acid derivatives were successfully capped. Synthesized ZnO-NPs in water have a zeta potential of -18.4 mV, showing that they are stable solutions. The ZnO-NPs proved to be highly toxic for the HepG2 cell line and showed a reduced cell viability of 23.68 ± 2.1 % after 24 hours of ZnO-NP treatment. ZnO-NPs also showed excellent inhibitory potential against the enzymes acetylcholinesterase (IC50: 102 μg/mL) and butyrylcholinesterase (IC50: 125 μg/mL) which are involved in Alzheimer’s disease. Overall, the enzymes involved in aging, diabetes, and inflammation showed a moderate inhibitory response to ZnO-NPs. Given these findings, these biosynthesized ZnO-NPs could be a good option for the cure of deadly diseases such as cancer, diabetes, Alzheimer’s, and other inflammatory diseases due to their strong anticancer potential and efficient antioxidant properties.

scholarly journals Isolation and characterisation of active principle from the leaves extract of Fadogia erythrophloea (K. Schum. & K. Krause) Hutch and Dalziel

2018 ◽  
Vol 11 (1) ◽  
pp. 41-46
Author(s):  
Abdullahi Sulaiman ◽  
G.I. Ndukwe ◽  
J.O. Amupitan
Keyword(s):  
Antimicrobial Activity ◽  
Research Work ◽  
Iridoid Glycoside ◽  
Active Principle ◽  
Spectroscopic Techniques ◽  
Plant Materials ◽  
In Vitro Antimicrobial Activity ◽  
The Family ◽  
Magnetic Resonances

Fadogia erythrophloea (K. Schum. & K. Krause) Hutch and Dalziel is a specie from the family of Rubiaceae. The plant materials of Fadogia erythrophloea have been used in Africa in alternative medicine to remedy dysentery, malaria, childhood fever, as antidotes (venomous stings, bites, etc.), emetics, vermifuge, colic and constipation. Therefore, this study was designed to isolate active principle(s) present in the leaves extract of Fadogia erythrophloea and to test for possible in-vitro antimicrobial activity. Isolation works were carried out using various chromatographic techniques on the n-butanol fraction; this yielded a pure compound labelled as ‘F.e’. From the data obtained through spectroscopic techniques using 1H NMR, 13C NMR, DEPT, COSY, NOESY, HMBC and HSQC Nuclear Magnetic Resonances (NMR),the compound ‘F.e’ was identified to be an ipolamiide(an iridoid glycoside). Based on the findings in this research work, it can be concluded that the leaves of the plant Fadogia erythrophloea (K.Schum. & K.Krause) Hutch. & Dalziel possess measurable in-vitro antimicrobial activity against many of the microorganisms implicated in the pathogenesis of human infections.Keywords: Fadogia erythrophloea, Rubiaceae, Isolation, spectroscopic techniques, iridoid glycoside, ipolamiide, in-vitro antimicrobial activity

Green Synthesis of Silver Nanoparticles by Allamanda cathartica L. Leaf Extract and Evaluation for Antimicrobial Activity

2013 ◽  
Vol 6 (4) ◽  
pp. 2260-2268
Author(s):  
M. Linga Rao ◽  
Bhumi G ◽  
Savithramma N
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Leaf Extract ◽  
Fusarium Species ◽  
Inhibition Zone ◽  
Fungal Species ◽  
Green Technology ◽  
Metallic Silver ◽  
Synthesis Of Nanoparticles ◽  
Aqueous Leaf Extract

Silver nanoparticles (SNPs) exhibit tremendous applications in medicine as antimicrobial agent.  The use of different parts of plants for the synthesis of nanoparticles is considered as a green technology as it does not involve any harmful chemicals.  In the present study, we report a rapid biosynthesis of silver nanoparticles from aqueous leaf extract of medicinal plant Allamanda cathartica.  The active phytochemicals present in the plant were responsible for the quick reduction of silver ion to metallic silver nanoparticles. The reduced silver nanoparticles were characterized by using UV-Vis spectrophotometry, Scanning Electron Microscope (SEM), Energy Dispersive Analysis of X-ray (EDAX) and Atomic Force Microscopy (AFM).  The spherical shaped silver nanoparticles were observed and it was found to 19-40 nm range of size.  These phytosynthesized SNPs were tested for their antimicrobial activity and it analyzed by measuring the inhibitory zone. A. cathartica aqueous leaf extract of SNPs showed highest toxicity to Pseudomonas followed by Klebsiella, Bacillus and E. coli and lowest toxicity towards Proteus. In fungal species, highest inhibition zone was noted against Rhizopus followed by Curvularia, Aspergillus flavus and Aspergillus niger and minimum inhibition zone was observed against Fusarium species.  These results suggest a promising potential of Indian plant-based green chemistry for production of SNPs for biomedical and nanotechnology applications.

scholarly journals Green Synthesis of CeO2 Nanoparticles from the Abelmoschus esculentus Extract: Evaluation of Antioxidant, Anticancer, Antibacterial, and Wound-Healing Activities

Molecules ◽  
2021 ◽  
Vol 26 (15) ◽  
pp. 4659
Author(s):  
Hafiz Ejaz Ahmed ◽  
Yasir Iqbal ◽  
Muhammad Hammad Aziz ◽  
Muhammad Atif ◽  
Zahida Batool ◽  
...  
Keyword(s):  
Wound Healing ◽  
Metal Oxide Nanoparticles ◽  
In Vitro Cytotoxicity ◽  
Ceo2 Nanoparticles ◽  
Abelmoschus Esculentus ◽  
Oxide Nanoparticles ◽  
Dependent Manner ◽  
Hydrogel Membrane ◽  
Cancerous Cells

Metal oxide nanoparticles synthesized by the biological method represent the most recent research in nanotechnology. This study reports the rapid and ecofriendly approach for the synthesis of CeO2 nanoparticles mediated using the Abelmoschus esculentus extract. The medicinal plant extract acts as both a reducing and stabilizing agent. The characterization of CeO2 NPs was performed by scanning electron microscopy (SEM), X-ray diffraction (XRD), ultraviolet-visible spectroscopy (UV-Vis), and Fourier transform infrared spectroscopy (FTIR). The in vitro cytotoxicity of green synthesized CeO2 was assessed against cervical cancerous cells (HeLa). The exposure of CeO2 to HeLa cells at 10–125 µg/mL caused a loss in cellular viability against cervical cancerous cells in a dose-dependent manner. The antibacterial activity of the CeO2 was assessed against S. aureus and K. pneumonia. A significant improvement in wound-healing progression was observed when cerium oxide nanoparticles were incorporated into the chitosan hydrogel membrane as a wound dressing.

scholarly journals Development of Antibacterial Protective Coatings Active against MSSA and MRSA on Biodegradable Polymers

Polymers ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 659
Author(s):  
Iva Rezić ◽  
Mislav Majdak ◽  
Vanja Ljoljić Bilić ◽  
Ivan Pokrovac ◽  
Lela Martinaga ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Biodegradable Polymers ◽  
Chronic Wounds ◽  
Protective Coatings ◽  
Metal Oxide Nanoparticles ◽  
Colloidal Solutions ◽  
Fused Deposition ◽  
Antimicrobial Nanoparticles ◽  
Mrsa Strains

In this work the in vitro antimicrobial activity of colloidal solutions of nine different commercially available nanoparticles were investigated against Staphylococcus aureus strains, both methicillin-sensitive (MSSA) and methicillin-resistant (MRSA). Research covered antimicrobial investigation of different metal and metal-oxide nanoparticles, including Ag 10 nm, Ag 40 nm, Al2O3 100 nm, Au 20 nm, Pt 4 nm, TiO2 100 nm, Y2O3 100 nm, ZnO 100 nm and ZrO2 100 nm nanoparticles. Such materials were foreseen to be applied as coatings on 3D-printed biodegradable polymers: i.e., catheters, disposable materials, hospital bedding items, disposable antimicrobial linings and bandages for chronic wounds. Therefore, the antimicrobial activity of the nanoparticles was determined by agar well diffusion assays and serial microdilution broth assays. In addition, the chromatographic characterization of elements present in trace amounts was performed as a method for tracing the nanoparticles. Moreover, the potential of preparing the rough surface of biodegradable polymers for coating with antimicrobial nanoparticles was tested by 3D-printing fused deposition methodology. The in vitro results have shown that particular nanoparticles provided powerful antimicrobial effects against MSSA and MRSA strains, and can be easily applied on different biopolymers.

Sign in / Sign up

Export Citation Format

Share Document