scholarly journals Screening the Therapeutic Potential of Methanolic Stem Extract of Cissus arnottiana

2021 ◽  
Vol 14 (3) ◽  
pp. 1405-1413
Author(s):  
P. Selvi ◽  
S. Murugesh ◽  
R. Yuvarajan Yuvarajan ◽  
A. Rajasekar Rajasekar
Keyword(s):  
Pathogenic Bacteria ◽  
Methanolic Extract ◽  
Therapeutic Potential ◽  
Antioxidant Properties ◽  
Food Habit ◽  
Potential Candidate ◽  
Dpph Assay ◽  
Stem Extract ◽  
Ancient Times ◽  
Therapeutic Needs

Modern lifestyle, pollution, food habit, and stress have intensively enhanced the evolution of several diseases on human being. Medicinal plants are used from the ancient times for the therapeutic needs to cure various diseases as well as less toxic in nature. One of such plant is Cissus arnottiana which is used from the olden days which has been identified as an important medicine plant by many researchers all over the world. Cissus arnottiana contains phytochemicals such as alkaloids, tannins, terpenoids, flavonoids, phenolic compounds etc. In this present work methanolic stem extract of Cissus arnottiana is used to evaluate the antibacterial activity of gram negative and gram positive human pathogenic bacteria. DPPH assay is used to investigate the antioxidant properties of the methanolic extract. The anti-inflammatory activity of the extract has been studied using anti proteinase assay. The MTT cell proliferation assay is carried out against HeLa cell line in which 44% of cells are viable for the concentration of 100 µg/mL. Interestingly, the methanolic stem extract can be used as a potential candidate for new therapeutic applications.

Antioxidant and cytotoxic activity of stems of Smilax zeylanica in vitro

2015 ◽  
Vol 26 (5) ◽  
Author(s):  
Mohammad Nasir Uddin ◽  
Taksim Ahmed ◽  
Sanzida Pathan ◽  
Md. Mamun Al-Amin ◽  
Md. Sohel Rana
Keyword(s):  
Petroleum Ether ◽  
Methanolic Extract ◽  
Antioxidant Properties ◽  
In Vitro Assays ◽  
Dpph Assay ◽  
Cytotoxic Potential ◽  
Phytochemical Study ◽  
Methanolic Extracts ◽  
Standard Procedures

AbstractPlant-derived phytochemicals consisting of phenols and flavonoids possess antioxidant properties, eventually rendering a lucrative tool to scavenge reactive oxygen species. This study was carried out to evaluate in vitro antioxidant and cytotoxic potential of methanolic extract and petroleum ether extracts ofPhytochemical screening was done following standard procedures. Antioxidant activity was tested using several in vitro assays, viz., 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay, NO assay, HPreliminary phytochemical study revealed the presence of flavonoids and glycosides in both extracts. Methanolic extract was found to possess stronger antioxidant potential than petroleum ether extracts in all assays. The ICThese findings demonstrate that methanolic extracts could be considered as potential sources of natural antioxidant, whereas petroleum ether extracts could be explored for promising anticancer molecules.

scholarly journals Chemical profiling and antioxidant activity of Equisetum ramosissimum Desf. stem extract, a potential traditional medicinal plant for urinary tract infections

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Jeyalatchagan Sureshkumar ◽  
Singamoorthy Amalraj ◽  
Ramar Murugan ◽  
Anantharaj Tamilselvan ◽  
Jayaraj Krupa ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Urinary Tract ◽  
Medicinal Plant ◽  
Petroleum Ether ◽  
Urinary Tract Infections ◽  
Pathogenic Bacteria ◽  
Methanolic Extract ◽  
Chemical Profiling ◽  
Stem Extract ◽  
Tract Infections

Abstract Background Equisetum ramosissimum Desf. (E. ramosissimum) is a widely used traditional medicinal plant to treat urinary tract infections (UTIs) by ethnic people throughout the world. The utility of the plant in treating urinary-related disorders was evaluated against selected pathogenic bacteria which has major role in causing UTIs. Hence, the present study executed to extract phytochemicals like total phenolics and flavonoids, chemical profiling by GC–MS analysis and to test their antioxidant activity from stem extracts of E. ramosissimum. The extraction process was directed by petroleum ether, chloroform, ethyl acetate, methanol, and aqueous solvents. Results The GC–MS analysis yielded 24 phytoconstituents with linoleic acid, palmitic acid, nonacosane, hexahydrofarnesyl acetone, and octacosane as major compounds. Methanolic extract yielded maximum amount of phenolics (TPC) and flavonoids (TFC) with 600.02 ± 0.22 mg GAE/g and 631.38 ± 0.69 mg QE/g, respectively. Methanolic extract also exhibited notable free radical scavenging activity with an IC50 of 123.89 ± 0.73, 150.10 ± 1.02, 146.01 ± 0.54, and 63.73 ± 6.12 µg/mL for DPPH, FRAP, ABTS, and O2− assays, respectively. The minimum inhibitory concentration (MIC) required to inhibit the growth of tested pathogenic bacteria was observed in aqueous and methanolic extracts with the value being 31.25 µg/mL against R. equi and V. cholerae. As like, methanolic and petroleum ether extracts efficiently inhibited the growth of B. subtilis with the MIC of 31.25 µg/mL. Conclusion It was concluded that the notable effect of methanolic and aqueous extracts against the uropathogenic bacteria reported in this study supported the traditional uses of this plant in treating UTIs. The results acquired from this investigation revealed that E. ramosissimum stem extract might be considered as an interesting candidate in the development of antibacterial agent against UTIs coupled with antioxidant properties.

Anti-inflammatory Activity of Mollugo cerviana Methanolic Extract in LPS-induced Acute Inflammatory RAW 264.7 Macrophages

2021 ◽  
Vol 24 ◽  
Author(s):  
Robina Antony ◽  
Jijin Raveendran ◽  
Prabath Gopalakrishnan Biju
Keyword(s):  
Acute Inflammation ◽  
Methanolic Extract ◽  
Therapeutic Potential ◽  
Indian Subcontinent ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Raw 264.7 Cells ◽  
Raw 264.7 ◽  
Anti Inflammatory

Background: The management of acute inflammation, which arises from complex biological responses to harmful stimuli, is an important determinant in recovery of the system from an otherwise detrimental outcome such as septicemia. However, the side effects and limitations of current therapeutics necessitate the development of newer and safer alternatives. Mollugo cerviana is a common medicinal herb of the Indian subcontinent and has been traditionally used for its fever mitigating, anti-microbial and hepatoprotective action. We have already reported the rich presence of radical scavenging phytochemicals in the plant extracts and their strong antioxidant properties. Objective: In the present study, we have evaluated the anti-inflammatory effects of methanolic extract (ME) of the areal parts of M. cerviana in a lipopolysaccharide (LPS)-induced acute inflammatory cell culture model. Method: RAW 264.7 mouse macrophages cell were stimulated by the bacterial endotoxin LPS at a concentration of 1 µg/mL. Cytotoxicity and anti-inflammatory potential of ME were carried out. Results: M. cerviana extract concentration up to 150 µg/ml was found to be non-toxic to cells (MTT and NRU assay). LPS induces acute inflammation by binding to TLR-4 receptors, initiating downstream signalling cascade that result in pro-inflammatory cytokine secretion. Extract treatment at 100 µg/ml suppressed LPS-induced gene expression (qPCR) and secretion (ELISA) of pro-inflammatory cytokines IL-1β, IL-6 and TNF-α, and the chemokine CCL2, indicating a dampening of the acute inflammatory cascade. LPS-induced elevation of ROS level (DCFDA method) was significant reduced by extract treatment. Nitric oxide production, as indicated by nitrite level, was significantly reduced post extract treatment. Conclusion: From this study, it is demonstrated that M. cerviana methanolic extract has potent anti-inflammatory effect in the in vitro acute inflammation model of LPS-stimulated RAW 264.7 cells. There is not reported study so far on the anti-inflammatory properties of M. cerviana in an LPS-induced acute inflammatory model which closely mimics a human bacteremia response. Hence, this study highlights the therapeutic potential of this extract as a source of anti-inflammatory lead molecules.

scholarly journals Anti-Inflammatory and Antioxidant Properties of the Extract, Tiliroside, and Patuletin 3-O-β-D-Glucopyranoside from Pfaffia townsendii (Amaranthaceae)

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Wallace Ribeiro Corrêa ◽  
Alessandra Freitas Serain ◽  
Leticia Aranha Netto ◽  
Jane V. N. Marinho ◽  
Arielle Cristina Arena ◽  
...  
Keyword(s):  
Therapeutic Potential ◽  
Antioxidant Properties ◽  
Ethanolic Extract ◽  
Total Phenolic ◽  
Paw Edema ◽  
Dpph Assay ◽  
Isolation And Identification ◽  
Popular Medicine ◽  
Brazilian Ginseng ◽  
Anti Inflammatory

Brazilian ginseng, including Pfaffia townsendii, is used in popular medicine as a natural anti-inflammatory, tonic, analgesic, and antidiabetic agent. In this study, we investigated the chemical composition and evaluated the antioxidant and anti-inflammatory activities of the P. townsendii ethanolic extract as well as the major isolated glycoside flavonoids tiliroside and patuletin 3-O-β-D-glucopyranoside. Chromatographic techniques and spectroscopic analysis were used for the isolation and identification of the major compounds. The antioxidant potential was determined through DPPH and ORAC-FL assays. The total phenolic content was measured using Folin-Ciocalteu reagent. The anti-inflammatory activity was determined based on a model of paw edema and carrageenan- (Cg-) induced pleurisy. We identified three phenolic acids, one carboxylic acid and two flavonoids, patuletin 3-O-β-D-glucopyranoside, and tiliroside. The ethanol crude extracts, partitions and isolated flavonoids (4581 μmol of Trolox equivalents/g of extract in ORAC and a SC50 of approximately 31.9 μg/mL in the DPPH assay) demonstrated antioxidant activity, and the ethanolic extract as well as isolated flavonoids inhibited paw edema induced by Cg and leukocyte migration in the Cg-induced pleurisy model. The extract, tiliroside, and patuletin 3-O-β-D-glucopyranoside obtained from P. townsendii have therapeutic potential against oxidative stress-related and inflammatory disorders.

Physicochemical and Antioxidant Properties of Pizza Dough-base Enriched with Black Cumin (Nigella sativa) Extracts

2019 ◽  
Vol 15 (5) ◽  
pp. 508-516
Author(s):  
Muhammad J. Iqbal ◽  
Masood S. Butt ◽  
Iqra Saeed ◽  
Hafiz A.R. Suleria
Keyword(s):  
Phenolic Content ◽  
Therapeutic Potential ◽  
Nigella Sativa ◽  
Antioxidant Properties ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Pharmacological Activities ◽  
Black Cumin ◽  
Ancient Times ◽  
Better Than

Background: Changing lifestyles have paved the way towards various physiological dysfunctions. Phytochemicals derived from spices are being widely utilized in diet from ancient times to fight against these physiological dysfunctions owing to their therapeutic potential and high pharmacological activities. Methods: Methods: The current investigation was an attempt to explore the antioxidant potential, physicochemical, and sensory properties of black cumin (Nigella sativa) enriched pizza base that was developed by using conventional and supercritical extracts. Results: It was observed that the texture of product becomes harder during storage from 4.36±0.16 to 4.71±0.17 kg force. Nonetheless, supercritical extract pizza base got better hedonic scores compared to other treatments. Antioxidant potential of pizza base enriched with supercritical extracts (Total Phenolic Content 108.08±4.88 mg GAE/100g) was also better than control and conventional solvent extract enriched treatment as 63.24±3.03 and 95.34±3.66 mg GAE/100g, respectively. Conclusion: Conclusively, the results depicted that pizza base containing supercritical extract exhibited superior physiochemical, hedonic and antioxidant properties.

scholarly journals Antioxidant and Anti-Cancer Potentials ofRheum emodiRhizome Extracts

2011 ◽  
Vol 2011 ◽  
pp. 1-9 ◽  
Author(s):  
Venkatadri Rajkumar ◽  
Gunjan Guha ◽  
Rangasamy Ashok Kumar
Keyword(s):  
Cell Lines ◽  
Aqueous Extract ◽  
High Performance ◽  
Methanolic Extract ◽  
Therapeutic Potential ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Antioxidant Property ◽  
Cytotoxic Activities ◽  
Pbr322 Dna

The objective of this study was to determine antioxidant and cytotoxic efficacies of methanolic and aqueous extracts ofRheum emodiWall. ex Meissn. rhizome. 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and hydroxyl radical scavenging activities, inhibitory effect on lipid peroxidation and Fe3+reducing antioxidant property have been used to investigate antioxidant properties of the extracts. Cytotoxicity of the extracts was tested on MDA-MB-435S and Hep3B cell lines. Both extracts displayed extensive cytotoxicity to the tested cell lines. The extracts were studied for their ability to protect pBR322 DNA from damage by UV induced photolysis of H2O2. The aqueous extract, though inferior to methanolic extract in its antioxidant potential exhibited efficiency in DNA protection, while the methanolic extract failed to protect the DNA. The amount of total polyphenolics in the extracts was measured by spectrophotometric method. The methanolic extract contained higher polyphenolic contents than aqueous extract. Significant positive correlations were observed (P< .05) between results of phenolic content estimation and that of antioxidant assays. Hence, high-performance liquid chromatography analysis was performed to identify few major phenolic compounds that might be responsible for these therapeutic properties. These results indicate that rhizome ofR. emodipossesses antioxidant and cytotoxic activities and therefore have therapeutic potential.

Estimation of total phenolics and flavonoidal contents as well as in vitro antioxidant potential of Apium leptophyllum Pers.

2013 ◽  
Vol 59 (3) ◽  
pp. 37-50 ◽  
Author(s):  
Bhusan Sahoo Himanshu ◽  
Subrat Kumar Bhattamisra ◽  
Uttam Kumar Biswas ◽  
Rakesh Sagar
Keyword(s):  
Nitric Oxide ◽  
Methanolic Extract ◽  
Therapeutic Potential ◽  
Antioxidant Properties ◽  
The Body ◽  
Total Phenolic ◽  
Dependent Manner ◽  
Pharmaceutical Industries ◽  
Dose Dependent

Abstract At present, major causes of diseases is oxidative stress affecting both metabolic and physiological functions of the body. That is why there is a great need for investigation of nutritious food supplements for counteracting these oxidative stresses. Therefore, the aim of study was to evaluate the therapeutic potential of Apium leptophyllum Pers. fruits by estimating total phenolic as well as flavonoidal contents and antioxidant values. The collected fruits were extracted separately using different solvents like methanol, ethanol and water. Total phenolic and flavonoid contents were measured from the respective extracts and correlated with their antioxidant values. The antioxidant properties of various fruit extracts (12.5, 25, 50, 100 and 150 μg/ml) were evaluated by DPPH, hydroxyl, nitric oxide and superoxide scavenging assay and compared with ascorbic acid as a standard. All the extracts of A. leptophyllum were found to be dose dependent inhibition against these free radicals. Among all these extracts, the methanolic one was found better in the scavenging activity and followed dose-dependent manner against DPPH, hydroxyl radical, nitric oxide, superoxide anions with minimum IC50 values of 97.9, 89.02, 135.37, 127.73 μg/ml, respectively, and also observed more significant (p<0.01) as compared with standard Furthermore, total phenolic and flavonoidal contents were found highest in methanolic extract. The results obtained in this study clearly indicate that the methanolic extract of A. leptophyllum may be used as a new potential source of natural nutritional supplement in food or pharmaceutical industries due to rich source of phenolic, flavonoidal contents as well as antioxidant property

Structurally Related Edaravone Analogues: Synthesis, Antiradical, Antioxidant, and Copper-Chelating Properties

2020 ◽  
Vol 18 (10) ◽  
pp. 779-790 ◽  
Author(s):  
Alexandre LeBlanc ◽  
Miroslava Cuperlovic-Culf ◽  
Pier Jr. Morin ◽  
Mohamed Touaibia
Keyword(s):  
Oxidative Stress ◽  
Amyotrophic Lateral Sclerosis ◽  
Therapeutic Potential ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Therapeutic Options ◽  
Partial Charge ◽  
Associated Diseases ◽  
Significant Interest ◽  
Lateral Sclerosis

Background:: The current therapeutic options available to patients diagnosed with Amyotrophic Lateral Sclerosis (ALS) are limited and edaravone is a compound that has gained significant interest for its therapeutic potential in this condition. Objectives: : The current work was thus undertaken to synthesize and characterize a series of edaravone analogues. Methods: A total of 17 analogues were synthesized and characterized for their antioxidant properties, radical scavenging potential and copper-chelating capabilities. Results: Radical scavenging and copper-chelating properties were notably observed for edaravone. Analogues bearing hydrogen in position 1 and a phenyl at position 3 and a phenyl in both positions of pyrazol-5 (4H)-one displayed substantial radical scavenging, antioxidants and copper-chelating properties. High accessibility of electronegative groups combined with higher electronegativity and partial charge of the carbonyl moiety in edaravone might explain the observed difference in the activity of edaravone relative to the closely related analogues 6 and 7 bearing hydrogen at position 1 and a phenyl at position 3 (6) and a phenyl in both positions (7). Conclusion: Overall, this study reveals a subset of edaravone analogues with interesting properties. Further investigation of these compounds is foreseen in relevant models of oxidative stress-associated diseases in order to assess their therapeutic potential in such conditions.

Antiproliferative Effects of Ocimum basilicum Methanolic Extract and Fractions, Oleanolic Acid and 3-epi-Ursolic Acid

2020 ◽  
Vol 6 (2) ◽  
pp. 134-146 ◽  
Author(s):  
Kehkashan Arshad Qamar ◽  
Ahsana Dar Farooq ◽  
Bina S. Siddiqui ◽  
Nurul Kabir ◽  
Sabira Begum
Keyword(s):  
Ursolic Acid ◽  
Oleanolic Acid ◽  
Mitotic Spindle ◽  
Immunofluorescence Microscopy ◽  
Methanolic Extract ◽  
Folk Medicine ◽  
Ocimum Basilicum ◽  
Antiproliferative Effects ◽  
Ancient Times ◽  
Mcf 7

Aims: The aim of the current study was to identify active compound(s) responsible for the antiproliferative effects of O. basilicum and explore their underlying mechanism/s. Background: Plants have been the source of medicines for the treatment of various diseases since ancient times. Ocimum basilicum (Sweet Basil, Bobai Tulsi) has been used in the folk medicine for the treatment of human liver, spleen and stomach cancers. Background: Plants have been the source of medicines for the treatment of various diseases since ancient times. Ocimum basilicum (Sweet Basil, Bobai Tulsi) has been used in the folk medicine for the treatment of human liver, spleen and stomach cancers. Objective: To emphasize the importance of O. basilicum as a potential novel non-toxic alternative to the conventional anticancer therapy. Method: O. basilicum (aerial parts) methanolic extract and fractions were screened against HT-144, MCF-7, NCI-H460 and SF-268 human cancer cell lines using sulforhodamine B assay. The more active Petroleum Ether Insoluble (PEI) fraction was fractionated into six sub-fractions (OB-1 to OB-6). Four pure compounds (3-O-methyl ursolic acid, oleanolic acid, 3-epi-ursolic acid and ursolic acid) were isolated from the more potent sub-fraction OB- 6. Triple channel immunofluorescence microscopy was employed to observe the effects of methanolic extract, PEI fraction, sub-fractions OB-5 and OB-6, 3-epi-ursolic acid and oleanolic acid on the cytoskeleton and nuclei of MCF-7 cells. Result: The methanolic extract and the PEI fraction exhibited selectively greater growth inhibition against MCF-7 cell line (TGI: 56 and 36.2 µg/ml, respectively). By using triple channel immunofluorescence microscopy, it was observed that the methanolic extract, PEI fraction, sub-fraction OB-5 and 3-epi-ursolic acid induced irregular mitotic spindle formation and slowing of mitotic progression in MCF-7 cells while sub-fraction OB-6 induced mitotic arrest in the prophase stage. F-actin aggregation was also visible in PEI fraction, subfraction OB-5 and 3-epi-ursolic acid treated MCF-7 cells. Conclusion: These results emphasize the importance of O. basilicum as a potential novel non-toxic alternative to the conventional anticancer therapy and suggest that it inhibits the growth of MCF-7 cancer cells via multiple mechanisms such as interaction with the microtubules and mitotic spindle apparatus, and F-actin aggregation.

scholarly journals Flavonoids from the Genus Euphorbia: Isolation, Structure, Pharmacological Activities and Structure–Activity Relationships

2021 ◽  
Vol 14 (5) ◽  
pp. 428
Author(s):  
Douglas Kemboi Magozwi ◽  
Mmabatho Dinala ◽  
Nthabiseng Mokwana ◽  
Xavier Siwe-Noundou ◽  
Rui W. M. Krause ◽  
...  
Keyword(s):  
Therapeutic Potential ◽  
Antioxidant Properties ◽  
Structure Activity Relationship ◽  
Biological Properties ◽  
Therapeutic Agents ◽  
Skeletal Structure ◽  
Activity Relationship ◽  
Hydroxyl Groups ◽  
Structure Activity ◽  
Review Reports

Plants of the genus Euphorbia are widely distributed across temperate, tropical and subtropical regions of South America, Asia and Africa with established Ayurvedic, Chinese and Malay ethnomedical records. The present review reports the isolation, occurrence, phytochemistry, biological properties, therapeutic potential and structure–activity relationship of Euphorbia flavonoids for the period covering 2000–2020, while identifying potential areas for future studies aimed at development of new therapeutic agents from these plants. The findings suggest that the extracts and isolated flavonoids possess anticancer, antiproliferative, antimalarial, antibacterial, anti-venom, anti-inflammatory, anti-hepatitis and antioxidant properties and have different mechanisms of action against cancer cells. Of the investigated species, over 80 different types of flavonoids have been isolated to date. Most of the isolated flavonoids were flavonols and comprised simple O-substitution patterns, C-methylation and prenylation. Others had a glycoside, glycosidic linkages and a carbohydrate attached at either C-3 or C-7, and were designated as d-glucose, l-rhamnose or glucorhamnose. The structure–activity relationship studies showed that methylation of the hydroxyl groups on C-3 or C-7 reduces the activities while glycosylation loses the activity and that the parent skeletal structure is essential in retaining the activity. These constituents can therefore offer potential alternative scaffolds towards development of new Euphorbia-based therapeutic agents.

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