scholarly journals Differential Modulatory Effect of Epigallocatechin-3-Gallate in Suppression of Tumor Proliferation

2021 ◽  
Vol 14 (3) ◽  
pp. 1133-1142
Author(s):  
Ahmed Algazeery ◽  
Ashraf S. El-Sayed ◽  
Fatma M El-Deeb ◽  
Nomier MA
Keyword(s):  
Green Tea ◽  
Genomic Dna ◽  
Epigallocatechin Gallate ◽  
Protective Role ◽  
Major Constituent ◽  
Tumor Proliferation ◽  
Anti Inflammatory ◽  
Remarkable Progress ◽  
Strong Barrier ◽  
Inflammatory Effect

Despite the remarkable progress in selecting the chemotherapeutic drugs, most are expensive and associated with many adverse effects targeting both cancer and normal cells. The using of polyphenols as natural materials for chemoprevention is considered a promising approach in reducing the tumor proliferation.This study aims to investigate whether a difference between the use of green tea and its component Epigallocatechin-gallate (EGCG) in treatment and protection against tumor. Sixty female Swiss albino mice weighted 20–22 g divided into 6 groups (n=10).The tumor suppression of green tea and EGCG was mirrored by evaluating their antioxidant and the anti-inflammatory effect on tumor markers and DNA integrity.Our results showed that the administration of EGCG showed a significant elevation of both antioxidants and anti-inflammatory markers in serum of EAC-bearing animals and revealed its high curative power to protect than treat tumor growth. Moreover, genomic DNA fragmentations assay present EGCG as a modulatory agent in keeping genome integrity.The administration of green tea and its major constituent EGCG showed a significant a potent protective role in suppressing tumor proliferation than its use in treatment due to its antioxidant and anti-inflammatory effect and maintaining the integrity of underlying genomic DNA that make it a strong barrier which arrest the process of oncogensis.

Versatile Health Benefits of Catechin from Green Tea (Camellia sinensis)

2019 ◽  
Vol 15 (1) ◽  
pp. 3-10 ◽  
Author(s):  
Satheesh Babu Natarajan ◽  
Suriyakala Perumal Chandran ◽  
Sahar Husain Khan ◽  
Packiyaraj Natarajan ◽  
Karthiyaraj Rengarajan
Keyword(s):  
Green Tea ◽  
Camellia Sinensis ◽  
Health Benefits ◽  
Epigallocatechin Gallate ◽  
Extraction Methods ◽  
Major Constituent ◽  
Epicatechin Gallate ◽  
Wide Range ◽  
Green Tea Catechin ◽  
Anti Inflammatory

Background: Tea (Camellia sinensis, Theaceae) is the second most consumed beverage in the world. Green tea is the least processed and thus contain rich antioxidant level, and believed to have most of the health benefits. </p><p> Methods: We commenced to search bibliographic collection of peer reviewed research articles and review articles to meet the objective of this study. </p><p> Results: From this study, we found that the tea beverage contains catechins are believed to have a wide range of health benefits which includes neuroprotective, anti-inflammatory, antiulcer, antiviral, antibacterial, and anti-parasitic effects. The four major catechin compounds of green tea are epigallocatechin (EGC), epicatechin (EC), epigallocatechin gallate (EGCG), and epicatechin gallate (ECG), of which EGCG is the major constituent and representing 50-80% of the total catechin content. And also contain xanthine derivatives such as caffeine, theophylline, and theobromine, and the glutamide derivative theanine. It also contains many nutritional components, such as vitamin E, vitamin C, fluoride, and potassium. We sum up the various green tea phytoconstituents, extraction methods, and its medicinal applications. </p><p> Conclusion: In this review article, we have summarized the pharmacological importance of green tea catechin which includes antioxidant potential, anti-inflammatory, antimicrobial, anticancer, antidiabetic and cosmetic application.

scholarly journals Anti-inflammatory Activity of Camellia sinensis, l. Extract Cream Combined with Vitamin C as Antioxidant on Croton Oil-induced Inflamation in Male Mice Strain BALB/C

2017 ◽  
Vol 22 (2) ◽  
pp. 73
Author(s):  
Nining Sugihartini ◽  
Ratih Saridewi ◽  
Ulfa Ramdhani M ◽  
Fitri Rahmawanti ◽  
Sapto Yuliani ◽  
...  
Keyword(s):  
Vitamin C ◽  
Green Tea ◽  
Epigallocatechin Gallate ◽  
Inflammatory Cells ◽  
Green Tea Extract ◽  
Inflammatory Activity ◽  
Inflammatory Parameters ◽  
Cox 2 ◽  
Anti Inflammatory ◽  
Tea Extract

Green tea extract cream contains epigallocatechin gallate (EGCG) as the active ingredient for anti-inflammatory. Epigallocatechin gallate is easyly oxidized and able to reduce its effectivity as an anti-inflammatory. Therefore, an addition of antioxidants in order to increase its stability is required. The purpose of this study was to determine the effect of adding the antioxidant Vitamin C on the effectivity of green tea extract as an anti-inflammatory. This study uses 6 groups of male mice strain BALB/C which were given treatment as follows: normal control, negative control, base cream, green tea extract (0.2%), Vitamin C cream (1%) and green tea extract cream with addition of Vitamin C. The anti-inflammatory activity was evaluated based on the expression of COX-2, inflammatory cells and the thickness of the epidermis in the skin tissue of mice after given crotton oil (0.1%) on the back for the induction of inflammation. After treatment cream for 3 days, mice were sacrificed for histopathological tissue preparations made with hematoxylin eosin staining and immunohistochemistry COX-2. Data were analyzed statistically with one way Anova followed by t-test to determine differences between groups at a significance level of 0.05. The test results indicate that cream of green tea extract is higher in decreasing inflammatory parameters in comparison with cream of Vitamin C, except in the thickness of epidermal parameter. Green tea extract cream with the addition of Vitamin C is higher in reducing inflammatory parameters than cream of green tea extract or cream of Vitamin C. The decline percentage of cells that express COX-2, inflammatory cells and the thickness of the epidermis in the each of groups were cream of green tea extract:57.95%;53.75%;34.83%, cream of Vitamin C:48.76%;34.96%;34.27%, cream of green tea extract and Vitamin C:61,89%;65,54%;46.30%, respectively. Based on the results of this study, it can be concluded that anti-inflammatory activity of green tea extract cream increased due to the addition of 1% vitamin C as an antioxidant.

scholarly journals Gut microbiota from green tea polyphenol-dosed mice improves intestinal epithelial homeostasis and ameliorates experimental colitis

Microbiome ◽  
2021 ◽  
Vol 9 (1) ◽  
Author(s):  
Zhenhua Wu ◽  
Shimeng Huang ◽  
Tiantian Li ◽  
Na Li ◽  
Dandan Han ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Green Tea ◽  
Fecal Microbiota Transplantation ◽  
Experimental Colitis ◽  
Short Chain Fatty Acids ◽  
Dependent Manner ◽  
Barrier Integrity ◽  
Green Tea Polyphenol ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Abstract Background Alteration of the gut microbiota may contribute to the development of inflammatory bowel disease (IBD). Epigallocatechin-3-gallate (EGCG), a major bioactive constituent of green tea, is known to be beneficial in IBD alleviation. However, it is unclear whether the gut microbiota exerts an effect when EGCG attenuates IBD. Results We first explored the effect of oral or rectal EGCG delivery on the DSS-induced murine colitis. Our results revealed that anti-inflammatory effect and colonic barrier integrity were enhanced by oral, but not rectal, EGCG. We observed a distinct EGCG-mediated alteration in the gut microbiome by increasing Akkermansia abundance and butyrate production. Next, we demonstrated that the EGCG pre-supplementation induced similar beneficial outcomes to oral EGCG administration. Prophylactic EGCG attenuated colitis and significantly enriched short-chain fatty acids (SCFAs)-producing bacteria such as Akkermansia and SCFAs production in DSS-induced mice. To validate these discoveries, we performed fecal microbiota transplantation (FMT) and sterile fecal filtrate (SFF) to inoculate DSS-treated mice. Microbiota from EGCG-dosed mice alleviated the colitis over microbiota from control mice and SFF shown by superiorly anti-inflammatory effect and colonic barrier integrity, and also enriched bacteria such as Akkermansia and SCFAs. Collectively, the attenuation of colitis by oral EGCG suggests an intimate involvement of SCFAs-producing bacteria Akkermansia, and SCFAs, which was further demonstrated by prophylaxis and FMT. Conclusions This study provides the first data indicating that oral EGCG ameliorated the colonic inflammation in a gut microbiota-dependent manner. Our findings provide novel insights into EGCG-mediated remission of IBD and EGCG as a potential modulator for gut microbiota to prevent and treat IBD.

scholarly journals Enhanced anti-inflammatory effect of resveratrol and EPA in treated endotoxin-activated RAW 264.7 macrophages

2012 ◽  
Vol 108 (9) ◽  
pp. 1562-1573 ◽  
Author(s):  
Victor Pallarès ◽  
Damien Calay ◽  
Lídia Cedó ◽  
Anna Castell-Auví ◽  
Martine Raes ◽  
...  
Keyword(s):  
Epigallocatechin Gallate ◽  
No Production ◽  
Raw 264.7 ◽  
Fish Food ◽  
Antioxidant Genes ◽  
Raw 264.7 Macrophages ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Macrophages play an important role in immunogenic challenges by producing reactive oxygen species, NO and proinflammatory cytokines that can aggravate and propagate local inflammation. Multiple mechanisms regulate these inflammatory processes. NF-κB and activator protein 1 pathways are crucial in the expression of proinflammatory genes, such as TNF-α, IL-1 (α or β) and -6. Some polyphenols, which are present in beverages, vegetables and fruits, and PUFA, which are present in marine oils and fish food, possess anti-inflammatory effects in vivo and in vitro. Our aim in the present study was to assess whether polyphenols and PUFA have synergistic anti-inflammatory effects in murine macrophages in vitro. Inflammation in RAW 264.7 macrophages was induced by lipopolysaccharide at 100 ng/ml. The treatments with molecules were performed by co-incubation for 19 h. A NO production assay by Griess reaction, a phosphoprotein assay by Pathscan ELISA kit and gene expression analysis using the TaqMan® Low-density Array for ninety-one genes related to inflammation, oxidative stress and metabolism were performed to assess the synergistic anti-inflammatory effects of polyphenols, epigallocatechin gallate and resveratrol (Res; 2·5 μg/ml), and the PUFA, DHA and EPA (30 μm). Adding Res+EPA had an enhanced anti-inflammatory effect, in comparison with EPA and Res alone, leading to decreased NO levels; modulating the phospho-stress activated protein kinase/Jun N-terminal kinase (P-SAPK/JNK) level; down-regulating proinflammatory genes, such as IL, chemokines, transcription factors; and up-regulating several antioxidant genes. Therefore, this combination has a stronger anti-inflammatory effect than either of these molecules separately in RAW macrophages.

scholarly journals Neuroprotective Properties of the Standardized Extract fromCamellia sinensis(Green Tea) and Its Main Bioactive Components, Epicatechin and Epigallocatechin Gallate, in the 6-OHDA Model of Parkinson’s Disease

2015 ◽  
Vol 2015 ◽  
pp. 1-12 ◽  
Author(s):  
Natália Bitu Pinto ◽  
Bruno da Silva Alexandre ◽  
Kelly Rose Tavares Neves ◽  
Aline Holanda Silva ◽  
Luzia Kalyne A. M. Leal ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Green Tea ◽  
Biological Effects ◽  
Great Part ◽  
Epigallocatechin Gallate ◽  
Neuroprotective Effects ◽  
Male Wistar Rats ◽  
Anti Inflammatory ◽  
Neuroprotective Actions

Camellia sinensis(green tea) is largely consumed, mainly in Asia. It possesses several biological effects such as antioxidant and anti-inflammatory properties. The objectives were to investigate the neuroprotective actions of the standardized extract (CS), epicatechin (EC) and epigallocatechin gallate (EGCG), on a model of Parkinson’s disease. Male Wistar rats were divided into SO (sham-operated controls), untreated 6-OHDA-lesioned and 6-OHDA-lesioned treated for 2 weeks with CS (25, 50, or 100 mg/kg), EC (10 mg/kg), or EGCG (10 mg/kg) groups. One hour after the last administration, animals were submitted to behavioral tests and euthanized and their striata and hippocampi were dissected for neurochemical (DA, DOPAC, and HVA) and antioxidant activity determinations, as well as immunohistochemistry evaluations (TH, COX-2, and iNOS). The results showed that CS and catechins reverted behavioral changes, indicating neuroprotection manifested as decreased rotational behavior, increased locomotor activity, antidepressive effects, and improvement of cognitive dysfunction, as compared to the untreated 6-OHDA-lesioned group. Besides, CS, EP, and EGCG reversed the striatal oxidative stress and immunohistochemistry alterations. These results show that the neuroprotective effects of CS and its catechins are probably and in great part due to its powerful antioxidant and anti-inflammatory properties, pointing out their potential for the prevention and treatment of PD.

scholarly journals Therapeutic Potential of EGCG, a Green Tea Polyphenol, for Treatment of Coronavirus Diseases

Life ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 197
Author(s):  
Junsoo Park ◽  
Rackhyun Park ◽  
Minsu Jang ◽  
Yea-In Park
Keyword(s):  
Green Tea ◽  
Therapeutic Potential ◽  
Epigallocatechin Gallate ◽  
Major Constituent ◽  
Future Research ◽  
Tea Polyphenol ◽  
Egcg Treatment ◽  
Main Protease ◽  
Green Tea Polyphenol ◽  
3Cl Protease

Epigallocatechin gallate (EGCG) is a major catechin found in green tea, and there is mounting evidence that EGCG is potentially useful for the treatment of coronavirus diseases, including coronavirus disease 2019 (COVID-19). Coronaviruses encode polyproteins that are cleaved by 3CL protease (the main protease) for maturation. Therefore, 3CL protease is regarded as the main target of antivirals against coronaviruses. EGCG is a major constituent of brewed green tea, and several studies have reported that EGCG inhibits the enzymatic activity of the coronavirus 3CL protease. Moreover, EGCG has been reported to regulate other potential targets, such as RNA-dependent RNA polymerase and the viral spike protein. Finally, recent studies have demonstrated that EGCG treatment interferes with the replication of coronavirus. In addition, the bioavailability of EGCG and future research prospects are discussed.

Anti-inflammatory effect of epigallocatechin gallate in a mouse model of ovalbumin-induced allergic rhinitis

2017 ◽  
Vol 49 ◽  
pp. 102-108 ◽  
Author(s):  
Meng Fu ◽  
Shulian Fu ◽  
Saihong Ni ◽  
Liyuan Zou ◽  
Yumei Liu ◽  
...  
Keyword(s):  
Allergic Rhinitis ◽  
Mouse Model ◽  
Epigallocatechin Gallate ◽  
Anti Inflammatory ◽  
Inflammatory Effect

scholarly journals Caftaric acid: an overview on its structure, daily consumption, bioavailability and pharmacological effects

2020 ◽  
Vol 10 (3) ◽  
pp. 5616-5623 ◽  
Keyword(s):  
Total Concentration ◽  
Grape Juice ◽  
Major Constituent ◽  
Total Phenolic ◽  
Pharmacological Effects ◽  
Daily Consumption ◽  
High Blood Glucose ◽  
Caftaric Acid ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Caftaric acid is simply known as phenolic derivative and it is present in high concentrations in grape seeds and juice. The chemical structure of caftaric acid is C13H12O9, with molar mass of the acid equal to 312.230 g/mol. Caftaric acid is formed united of caffeic acid and tartaric acid. This paper reviews caftaric acid structure, daily consumption, bioavailability and pharmacological effects. The caftaric acid quickly passes to the stomach and duodenum and increases the absorption of the acid in the intestinal Caco-2 cells. The antioxidant effect of grape stem was related to its caftaric acid constituent. The grape juice has anti-inflammatory effect and this anti-inflammatory effect is correlated with the main constituent of this juice which is caftaric acid. Caftaric acid has antimutagenic effect in an animal model suggesting that caftaric acid participates in chemopreventive effect of the Yamabudo juice. The "liver detoxifying" effect is observed and correlated with oral supplementation with aqueous decoctions of Cichorium spinosum and Cichorium intybus in Greece where caftaric acid is the major constituent of these two aqueous decoctions. The caftaric acid possesses a double effect through decreasing high blood glucose and high blood pressure so this acid can be used in the treatment of diabetes and hypertension. The caftaric acid increases granulocyte/macrophage-colony forming cells from femurs of female animals' models by 70%. In conclusion, caftaric acid is presented about 5 mg/100 cm3 grape juice. The acid is quickly passes into the stomach and duodenum. The trans-caftaric acid represents 85% of the total phenolic content in the Concord grape juice with a total concentration of 444 μmol/L. The caftaric acid has many pharmacological effects such as antioxidant, anti-inflammatory, antimutagenic and anticarcinogenic, hepatoprotective, anti-diabetic and anti-hypertensive, anti-obesity and anti-metabolic syndrome and neuroprotective effects.

scholarly journals Pro-apoptotic and anti-inflammatory properties of the green tea constituent epigallocatechin gallate increase photodynamic therapy responsiveness

2011 ◽  
Vol 43 (7) ◽  
pp. 644-650 ◽  
Author(s):  
Angela Ferrario ◽  
Marian Luna ◽  
Natalie Rucker ◽  
Sam Wong ◽  
Charles J. Gomer
Keyword(s):  
Photodynamic Therapy ◽  
Green Tea ◽  
Epigallocatechin Gallate ◽  
Anti Inflammatory
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