scholarly journals Silver Dots – A Screening and Prognostic Aid in Oral Health

2021 ◽  
Vol 14 (01) ◽  
pp. 513-517
Author(s):  
S. Prasanna ◽  
Srikant. N
Keyword(s):  
Prognostic Value ◽  
Normal Subjects ◽  
Proliferative Index ◽  
Oral Epithelial Cells ◽  
Glass Slides ◽  
Buccal Smear ◽  
Agnor Staining ◽  
Oral Epithelial ◽  
Diagnostic And Prognostic Value ◽  
Histopathological Grading

Proper histopathological grading and typing of a tumour plays a significant role in evaluating and assessing the clinical management and prognosis of the tumour. Microscopically sometimes it is difficult in categorizing a tumour into benign or malignant as histopathology does not depict all the features which are of diagnostic and prognostic value. The present study aims :To evaluate the proliferative index of the oral epithelial cells taken from a buccal smear. Materials and method : A total of 90 subjects were included with 30 subjects in each category of normal, smokers and tobacco chewers. The smears were collected from buccal mucosa and applied on the glass slides followed by fixation with alcohol for 30 min and staining of the slides with AgNOR staining as proposed by Bukhari et al (2007). Results: The AgNOR number where more in smokers when compared to normal subjects and was statistically significant. Similarly in chewers it was also comparatively higher when compared to normal and statistically significant. But the AgNOR counts between smoker and tobacco chewer, showed a mean difference of 0.6 and was not statistically significant.

scholarly journals Age and AgNor- A Morphometric study

2020 ◽  
Vol 1 (1) ◽  
Author(s):  
Manpreet Kaur ◽  
Smita Naik ◽  
Sachin Jindal
Keyword(s):  
Epithelial Cells ◽  
Buccal Mucosa ◽  
Age Groups ◽  
Morphometric Study ◽  
Healthy Individuals ◽  
Oral Epithelial Cells ◽  
Glass Slides ◽  
Buccal Epithelial Cells ◽  
Oral Epithelial ◽  
Ribosome Biosynthesis

To investigate and correlate total AgNOR area/Total nucleus area (TAA/TNA ) values in buccal epithelial cells of healthy individuals in different age groups. Material and Methodology:- In present study 50 healthy individuals are included with age ranging 10 to 60 years. These are divided into 5 groups.Group1-10-20year, Group2-20- 30year, Group3-30-40year, Group4-40-50year, Group5-50-60year. Oral epithelial cells collected with the help of cyto brush from buccal mucosa. Smears were prepared on clean glass slides and fixed with 95% alcohol. Fixed slides Smears were stained with AgNOR stain. Results: Statistically significant correlations were found between mean TAA/TNA values and age p < 0.001 for linear and p < 0.0001 for polynominal regression, and between AgNOR number and age, p < 0.001 for linear. Conclusion: There is a significant correlation between age and AgNOR amount (ribosome biosynthesis rate) in buccal epithelial cells of healthy individuals. AgNORs in buccal epithelial cells may be used for detection of age.

THE ASSESSMENT OF DIAGNOSTIC AND PROGNOSTIC VALUE OF IMMUNOLOGIC BIOMARKERS IN PATIENTS WITH MYOCARDITIS

2019 ◽  
Vol 15 (68) ◽  
pp. 034
Author(s):  
T. I. Gavrilenko ◽  
S. V. Cherniuk ◽  
O. A. Pidgaina ◽  
N. A. Ryzhkova ◽  
L. V. Yakushko
Keyword(s):  
Prognostic Value ◽  
Diagnostic And Prognostic Value

Platelets in Atherothrombosis - Diagnostic and Prognostic Value of Platelet Activation in Patients with Atherosclerotic Diseases

2012 ◽  
Vol 10 (5) ◽  
pp. 589-596 ◽  
Author(s):  
Boris Bigalke ◽  
Andreas Schuster ◽  
Kateryna Sopova ◽  
Thomas Wurster ◽  
Konstantinos Stellos
Keyword(s):  
Platelet Activation ◽  
Prognostic Value ◽  
Diagnostic And Prognostic Value

Diagnostic and Prognostic Value of 18F-FDG PET/CT in Male Breast Cancer: Results From a Bicentric Population

2016 ◽  
Vol 9 (2) ◽  
pp. 169-177 ◽  
Author(s):  
Laura Evangelista ◽  
Francesco Bertagna ◽  
Mattia Bertoli ◽  
Tigu Stela ◽  
Giorgio Saladini ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Male Breast Cancer ◽  
Fdg Pet ◽  
Prognostic Value ◽  
Male Breast ◽  
Pet Ct ◽  
18F Fdg ◽  
Fdg Pet Ct ◽  
Diagnostic And Prognostic Value

scholarly journals Novel Aggregation Properties of Candida albicans Secreted Aspartyl Proteinase Sap6 Mediate Virulence in Oral Candidiasis

2015 ◽  
Vol 83 (7) ◽  
pp. 2614-2626 ◽  
Author(s):  
Rohitashw Kumar ◽  
Darpan Saraswat ◽  
Swetha Tati ◽  
Mira Edgerton
Keyword(s):  
Candida Albicans ◽  
Epithelial Cells ◽  
Oral Candidiasis ◽  
Oral Microbiome ◽  
Proteinase Activity ◽  
Adhesion Properties ◽  
Content Type ◽  
Oral Epithelial Cells ◽  
Oral Epithelial ◽  
Cell Cell

Candida albicans, a commensal fungus of the oral microbiome, causes oral candidiasis in humans with localized or systemic immune deficiencies. Secreted aspartic proteinases (Saps) are a family of 10 related proteases and are virulence factors due to their proteolytic activity, as well as their roles in adherence and colonization of host tissues. We found that mice infected sublingually withC. albicanscells overexpressing Sap6 (SAP6OE and a Δsap8strain) had thicker fungal plaques and more severe oral infection, while infection with the Δsap6strain was attenuated. These hypervirulent strains had highly aggregative colony structurein vitroand higher secreted proteinase activity; however, the levels of proteinase activity ofC. albicansSaps did not uniformly match their abilities to damage cultured oral epithelial cells (SCC-15 cells). Hyphal induction in cells overexpressing Sap6 (SAP6OE and Δsap8cells) resulted in formation of large cell-cell aggregates. These aggregates could be produced in germinated wild-type cells by addition of native or heat-inactivated Sap6. Sap6 bound only to germinated cells and increasedC. albicansadhesion to oral epithelial cells. The adhesion properties of Sap6 were lost upon deletion of its integrin-binding motif (RGD) and could be inhibited by addition of RGD peptide or anti-integrin antibodies. Thus, Sap6 (but not Sap5) has an alternative novel function in cell-cell aggregation, independent of its proteinase activity, to promote infection and virulence in oral candidiasis.

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