scholarly journals Protective Effect of Sodium Tetraborate on Chromium-induced Brain Damage in Rats

2018 ◽  
Vol 11 (1) ◽  
pp. 227-236 ◽  
Author(s):  
Yerbolat Iztleuov ◽  
Talgar Abilov ◽  
Ganiya Zhanabayeva ◽  
Irina Ismailova ◽  
Marat Iztleuov
Keyword(s):  
Body Weight ◽  
Intraperitoneal Injection ◽  
Wistar Rats ◽  
Neuroprotective Effect ◽  
Sodium Tetraborate ◽  
High Toxicity ◽  
Protein Thiol ◽  
Group 4 ◽  
Single Intraperitoneal Injection ◽  
Mda Content

Hexavalent chromium presents a particular threat due to its high toxicity. In this study showed the neuroprotective effect of sodium tetraborate in chromic intoxication. The experiment was performed on Wistar rats divided into 4 groups: 1 group - control; 2 group - single intraperitoneal injection of K2Сr2O7 in a dose of 0,5LD50, in the 3 and 4 groups with drinking water received Na2B4O7. Animals of the 3 group - 4 mg/kg of body weight, in 4 group - 72 mg/kg of body weight daily for 10 days, the last administration of Na2B4O7 was combined with a single intraperitoneal injection of K2Сr2O7 at a dose of 14 mg/kg (0,5LD50). Na2B4O7 in a dose of 4 mg/kg leads to a decrease in the level of MDA by 33%, an increase in the activity of catalase by 69%, superoxide dismutase by 21%, GR by 49%. Na2B4O7 at a dose of 72 mg/kg increases the MDA content by 31% in comparison with the data of rats of chromic intoxication. K2Сr2O7 reduces the GSH level by 42%, the non-protein thiol by 36%. Na2B4O7 at a dose of 4 mg/kg increased the GSH level by 53%, the non-protein thiol by 35%, and at a dose of 72 mg/kg reduced the GSH content by 23% (ρ<0.05), the non-protein thiol by 20% (ρ<0.05) in comparison with the data of rats exposed to K2Сr2O7.

Neuroprotection by glucagon: role of gluconeogenesis

2011 ◽  
Vol 114 (1) ◽  
pp. 85-91 ◽  
Author(s):  
Rami Abu Fanne ◽  
Taher Nassar ◽  
Achinoam Mazuz ◽  
Otailah Waked ◽  
Samuel N. Heyman ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Amino Acid ◽  
Intraperitoneal Injection ◽  
Neuroprotective Effect ◽  
Closed Head Injury ◽  
Lesion Size ◽  
Hepatic Uptake ◽  
Glucose Levels ◽  
Single Intraperitoneal Injection ◽  
Amino Acid Glutamate

Object The severity of neurological impairment following traumatic brain injury (TBI) is exacerbated by several endogenous processes, including hyperglycemia, hypotension, and the generation of glutamate. However, in addition to controlling hyperglycemia, insulin has pleiotropic effects on tissue metabolism, which include reducing the concentration of the neurotoxic amino acid glutamate, making it unclear whether insulin's beneficial effects are attributable to the establishment of euglycemia per se. In the present study, the authors asked if reducing glutamate via approaches that do not lower glucose levels would improve neurological outcome following TBI. Methods Glucagon activates gluconeogenesis by increasing the hepatic uptake of amino acids such as glutamate and facilitating their conversion to glucose. Glucagon was administered as a single intraperitoneal injection before or after closed head injury (CHI). Neurological function, brain histological features, blood glutamate and glucose levels, and CSF glutamate concentrations were measured. Results A single intraperitoneal injection of glucagon (25 μg) into mice 10 minutes before or after CHI reduced lesion size by about 60% (p < 0.0001) and accelerated neurological recovery. The neuroprotective effect of glucagon was related to gluconeogenesis by decreasing the concentration of the neuroexcitatory amino acid glutamate in the circulation from 207 ± 32.1 μmol/L in untreated mice to 101.11 ± 21.6 μmol/L in treated mice (p < 0.001); a similar effect occurred in the CSF. The neuroprotective effect of glucagon was seen notwithstanding the attendant increase in blood glucose, the final substrate of gluconeogenesis. Conclusions Glucagon exerts a marked neuroprotective effect post-TBI by decreasing CNS glutamate. Glucagon was beneficial despite increasing blood glucose. Favorable effects also occurred when glucagon was given prior to TBI, suggesting its involvement in the preconditioning process. Thus, glucagon may be of value in providing neuroprotection when administered after TBI or prior to certain neurosurgical or cardiac interventions in which the incidence of perioperative ischemia is high.

Experimental chronic subdural hematoma in mice

1987 ◽  
Vol 67 (5) ◽  
pp. 710-716 ◽  
Author(s):  
Hisashi Aikawa ◽  
Kinuko Suzuki
Keyword(s):  
Body Weight ◽  
Blood Vessels ◽  
Subdural Hematoma ◽  
Intraperitoneal Injection ◽  
Intraventricular Hemorrhage ◽  
Chronic Subdural Hematoma ◽  
Content Type ◽  
Single Intraperitoneal Injection ◽  
Thin Walled ◽  
Fine Print

✓ A new experimental model of chronic subdural hematoma in mice is described. A single intraperitoneal injection of 6-aminonicotinamide (25 mg/kg body weight) on the 5th postnatal day induced hydrocephalus in mice with almost 100% success. Approximately 60% of the mice spontaneously developed intracranial hemorrhage 20 days after the injection. About 1 week after the hemorrhage, a lens-shaped or spherical subdural hematoma was observed, accompanied by marked dilatation of the lateral ventricles and intraventricular hemorrhage. Histological examination revealed that the hematoma contained well-organized outer and inner membranes. Fresh hemorrhage surrounded by many hemosiderin-laden macrophages was seen at the margin of the hematoma adjacent to the organizing outer membrane, in which many fibroblasts and blood vessels were noted. The inner membrane of the hematoma was made up of several tiers of flattened cells with thin-walled blood vessels. The gross morphology and histology of these hematomas closely resembled those of human chronic subdural hematoma.

scholarly journals HYPOGLYCEMIC EFFECT OF ANDROGRAPHIS ECHIOIDES ON STREPTOZOTOCIN INDUCED EXPERIMENTAL RATS

2015 ◽  
Vol 7 ◽  
pp. 55
Author(s):  
Mani Rupeshkumar ◽  
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Wistar Rats ◽  
Methanol Extract ◽  
Fasting Blood Glucose ◽  
Hypoglycemic Effect ◽  
Reference Drug ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Single Intraperitoneal Injection

The present study aims to study the hypoglycemic effect of methanol extract of Andrographisechioides (MEAE) in streptozotocin (STZ)-induced diabetic Wistar rats. Hyperglycemia was induced in rats by single intraperitoneal injection of STZ (55 mg/kg bodyweight). Three days after STZ induction, the hyperglycemic rats were treated with MEAE orally at the doses of 200, 500, and 800 mg/kg body weight daily for 21 days. Glibenclamide (1 mg/kg, orally) was used as reference drug. The fasting blood glucose levels were measured on each 7th day during the 21 days of treatment.

scholarly journals Acute Toxicity and Dose Fixation Studies on Chloroxylon swietenia Dc Bark Extracts on Streptozotocin Induced Diabetic Rats

2015 ◽  
Vol 48 ◽  
pp. 8-13 ◽  
Author(s):  
B. Jayaprasad ◽  
P.S. Sharavanan ◽  
R. Sivaraj
Keyword(s):  
Diabetes Mellitus ◽  
Metabolic Disease ◽  
Body Weight ◽  
Acute Toxicity ◽  
Wistar Rats ◽  
Diabetic Rats ◽  
Aqueous Extracts ◽  
Single Intraperitoneal Injection ◽  
Toxic Reactions ◽  
Chloroxylon Swietenia

Diabetes mellitus (DM) is a chronic metabolic disease with the highest rates of prevalence and mortality worldwide. Chloroxylonswietenia has been used extensively in folkloric medicine. The present study aims to determine the acute toxicity of Chloroxylonswietenia bark methanol (CSBMEt) and aqueous extracts (CSBAEt) (100, 150, 250, 500 and 1000 mg/kg body weight) and dose fixation of CSBMEt and CSBAEt in streptozotocin induced diabetic rats. Diabetes was induced in male albino wistar rats by single intraperitoneal injection of streptozotocin (50mg/kg b.w). The diabetic rats were administered with Chloroxylonswietenia bark extracts (CSBMEt and CSBAEt) (75,125 and 250mg/kg b.w) orally by intragastric intubation for 15 days. Acute toxicity studies revealed the non-toxic nature of the CSBMEt and CSBAEt. No lethality or toxic reactions found at any doses until the end of study, whereas 75 and 125 mg/kg b.w. doses of CSBMEt and CSBAEt produce no significant changes in the diabetic rats and 250mg/kg b.w. of CSBMEt and CSBAEt have significant change in the blood glucose. The results conclude that, there was no toxicity observed up to 1000mg/kg b.w. of both the extracts and 250mg/kg b.w. of CSBMEt and CSBAEt can be used as effective dose to treat diabetes.

scholarly journals GASTROPROTECTIVE EFFECT OF ONION PEEL (Allium Cepa L. Var Ascalonicum) EXTRACT ON WISTAR RATS INDUCED BY MEFENAMIC ACID

2020 ◽  
Vol 3 (1) ◽  
pp. 31
Author(s):  
Awalya Rahma Putri ◽  
Dina Helianti ◽  
Nindya Shinta Rumastika
Keyword(s):  
Body Weight ◽  
Mefenamic Acid ◽  
Wistar Rats ◽  
Mucosal Damage ◽  
Gastric Mucosal Damage ◽  
Weight Group ◽  
Onion Peel ◽  
Group 4 ◽  
Gastroprotective Effect ◽  
Group 1

Non Steroidal Anti-inflammatory Drugs (NSAID) have been implicated in the etiology of gastritis. Mefenamic acid is a non-selective NSAID used to treat mild to moderate pain and inflammation. Mefenamic acid induces gastritis through its inhibition of prostaglandin that results in increased HCl secretion, decreased mucin, and bicarbonate ion production. This study aimed to investigate the gastroprotective effect of onion peel extract (OPE) against mefenamic acid-induced gastritis. In this study, the gastroprotective effect of OPE was examined through mucosal integrity scoring. A total of 28 male Wistar rats were divided into 4 groups, normal (Group 1), mefenamic acid+NaCMC 0,5% (Group 2), mefenamic acid+OPE 600mg/kg body weight (Group 3), and mefenamic acid+OPE 1200mg/kg body weight (Group 4). All rats were sacrificed on day 15 then all gasters were collected. Histopathological examination was done under a microscope with 100X magnification. Administration of mefenamic acid without OPE at all significantly increased gastric mucosal damage (p<0,05). Administration of OPE 1200mg/kg body weight significantly decreased gastric mucosal damage (p<0,05). Group 4 shows no significant difference (p=0,66) with the normal group (Group 1). This indicates that administration of OPE 1200mg/kg body weight per oral ameliorates mefenamic acid-induced gastritis.

scholarly journals Long-term CCK-leptin synergy suggests a role for CCK in the regulation of body weight

1999 ◽  
Vol 276 (4) ◽  
pp. R1038-R1045 ◽  
Author(s):  
Claire A. Matson ◽  
Robert C. Ritter
Keyword(s):  
Body Weight ◽  
Food Intake ◽  
Intraperitoneal Injection ◽  
Sucrose Intake ◽  
Reduced Body ◽  
Single Intraperitoneal Injection

The gut peptide CCK is a nutrient-related signal important to the control of food intake. In the present studies, we observed that a single intraperitoneal injection of CCK (1–2 μg/kg) given 2–3 h after intracerebroventricular leptin (2–5 μg) reduced body weight and chow intake over the ensuing 48 h more than did leptin alone. CCK alone had no effect on either 48-h chow intake or body weight but significantly reduced feeding during a 30-min sucrose test. However, reduction of 30-min sucrose intake by CCK was not enhanced by prior intracerebroventricular leptin. The present data suggest that CCK can contribute to the regulation of body weight when central leptin levels are elevated.

scholarly journals Morphology of bronchial epithelium in rodent streptozotocin-induced diabetes mellitus

2013 ◽  
Vol 16 (4) ◽  
pp. 44-48
Author(s):  
Oksana Anatolyevna Pivovarova ◽  
Boris Nikitich Mankovsky
Keyword(s):  
Diabetes Mellitus ◽  
Body Weight ◽  
Wistar Rats ◽  
Bronchial Epithelium ◽  
Secretory Cells ◽  
Control Group ◽  
Partial Loss ◽  
Citrate Buffer ◽  
Single Intraperitoneal Injection ◽  
Streptozotocin Induced Diabetes

Aim.  To study the morphology of bronchial epithelium in a rodent streptozotocin-induced (STZ) diabetes mellitus. Materials and Methods. . Diabetes mellitus was introduced in 47 white Wistar rats aged 5?6 months (body weight 234.0?2.64 g). 43 white Wistar rats of the same age were used as control subjects (body weight 242.0?2.13). Diabetes was induced by single intraperitoneal injection of STZ (SIGMA, USA) 60 mg/kg in 0.1 M citrate buffer, pH 4.5. Results. A statistically significant decrease in the total epithelial area by 25.9% was observed in the study group, accompanied by a reduction of the supranuclear zone by 22.1% vs. the control group. Conclusion. We found that bronchial mucous membrane in rodents with STZ-induced diabetes mellitus exhibits signs of atrophy and partial loss of mucous production by bronchial secretory cells.

scholarly journals Preliminary Study on the Effect of Methanolic Extract of Watermelon (Citrullus lanatus) Rind on Prednisolone Suppressed Immunity in Male Wistar Rats

2021 ◽  
pp. 1-6
Author(s):  
Nyejirime Young Wike ◽  
Mobisson Samuel Kelechi ◽  
Godspower Onyeso ◽  
Okekem Amadi ◽  
Elizabeth Eepho Krukru
Keyword(s):  
Body Weight ◽  
Wistar Rats ◽  
Methanolic Extract ◽  
Citrullus Lanatus ◽  
Control Group ◽  
Group 4 ◽  
Watermelon Rind ◽  
Male Wistar Rats ◽  
Group 3 ◽  
Group 2

Citrullus lanatus thumb (Cucurbitaceae) commonly called watermelon is widely consumed in this part of the world as food and medicine. This study was carried out to examine the effect of methanolic extract of watermelon (Citrullus lanatus) rind on prednisolone suppressed immunity in male wistar rats. A total of 20 male wistar rats weighing 150-294g were used in 4 groups with five rats each. Group 1, the control group was given distilled water and feed, Group 2 was given 200 mg/kg body weight of methanolic extract of watermelon rind, Group 3 rats were given 2.5 mg/kg body weight of prednisolone and Group 4 rats were given 2.5 mg/kg body weight of prednisolone and 200 mg/kg body weight of methanolic extract of watermelon rind. Prednisolone and the methanolic extract of watermelon rind were administered orally for a period of 30 days. Blood samples were collected by cardio puncture from the rats for white blood cell (WBC), lymphocyte, and granulocyte and monocyte counts at the end of the experiment.  The data were statistically analysed using one-way ANOVA (Analysis of variance). Data were considered significant at p<0.05. The results obtained showed that methanolic extract of watermelon rind caused a significant increasen in immune function of rats when compared with the control and immune suppressed rats.

Evidence for a Decreased Noradrenaline Sensitivity in Mesenteric Resistance Vessels from Rats with Chemical Renal Medullectomy

1989 ◽  
Vol 77 (2) ◽  
pp. 167-170 ◽  
Author(s):  
S. J. Bund ◽  
A. M. Heagerty ◽  
A. Fletcher ◽  
J. D. Swales
Keyword(s):  
Blood Pressure ◽  
Body Weight ◽  
Intraperitoneal Injection ◽  
Wistar Rats ◽  
Vascular Reactivity ◽  
Morphological Changes ◽  
Pressor Responses ◽  
Resistance Vessels

1. Chemical renal medullectomy was performed in Wistar rats by intraperitoneal injection of 2-bromoethylamine hydrobromide (200 mg/kg body weight). The effect of this treatment upon blood pressure and mesenteric vascular reactivity and morphology was studied 3 weeks after treatment. 2. Blood pressure was significantly raised in medull-ectomized rats. The indirect systolic pressures (means ± sem) were 112 ± 2.4 mmHg (14.9 ± 0.32 kPa) and 123 ± 3.5 mmHg (16.4 ± 0.5 kPa) (P < 0.02) for control (n = 12) and medullectomized (n = 12) rats, respectively. However, there were no significant changes in the morphology of the mesenteric resistance vessels. 3. Maximum pressor responses and Ca2+ sensitivities were unchanged in these vessels compared with controls, but the sensitivity to noradrenaline was significantly reduced. Noradrenaline pD2 (= −log ED50) values (means ± sem) were 5.87 ± 0.03 and 5.69 ± 0.08 (P < 0.05) for control (n = 12) and medullectomized (n = 12) rats, respectively. 4. This study demonstrates that in this model of hypertension the noradrenaline sensitivity is reduced in mesenteric resistance vessels, but there are no detectable morphological changes associated with the rise in blood pressure.

scholarly journals Wistar Rat Weight Loss on Conventional Cigarette Exposure Compared to E-Cigarette

2020 ◽  
Vol 9 (3S) ◽  
pp. 279-281
Keyword(s):  
Weight Loss ◽  
Body Weight ◽  
Wistar Rats ◽  
Smoking Behavior ◽  
Wistar Rat ◽  
Weight Changes ◽  
Nicotine Content ◽  
Group 4 ◽  
Male Wistar Rats ◽  
Materials Used

Several studies have shown that smoking behavior is closely related to weight and obesity. Smokers tend to prefer fatty foods with high calories which ultimately makes a smoker vulnerable to being overweight. However, there are some studies that show lower body weight was found in smokers than nonsmokers. Our aim is to evaluate the short-term effects of smoking with conventional cigarettes and e-cigarret on weight changes in Wistar rats because animals try to minimize some confounding factors that are difficult to work on human samples (for example, physical activity, diet and motivation). This research is an experimental study, using 8-week-old male Wistar rats with body weight 150-200 grams divided randomly into 5 groups, namely 1) the group was not treated, 2) the filter cigarette exposure group, 3) the clove cigarette group , 4) e-cigarette group with a dose of 3 mL with nicotine content of 0 mg, and 5) ecigarette group with a dose of 3 mL with nicotine content of 3 mg. Body weight measurements using a digital scale. The results showed that there was a significant change in body weight between clove cigarettes and filter cigarettes with p = 0.024. In the results of this study, showed weight loss in all groups given cigarette smoke. Weight loss was significantly lower in the filter cigarette group compared to clove cigarettes and there was no significant weight difference between the conventional cigarette group and e-cigarette. This is likely the cigarette filter actually creates a major problem for smokers because of the design and materials used so it does not provide a safer effect for smoker.

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