scholarly journals Immunohistochemical Expression of p53 and Bcl-2 in Algerian Cervical Carcinoma

2018 ◽  
Vol 11 (1) ◽  
pp. 67-75
Author(s):  
Deffar Khalissa ◽  
Khenchouche Abdelhalim ◽  
Xing Xie ◽  
Ying Li ◽  
Ouhida Soraya ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Prognostic Marker ◽  
Uterine Cervix ◽  
Prognostic Markers ◽  
P53 Protein ◽  
Immunohistochemical Expression ◽  
Important Indicator ◽  
P53 Expression

The Objective of the present study is to evaluate the expression levels of Bcl-2 and p53 proteins in squamous cell carcinoma and adenocarcinoma of the uterine cervix, and try to explain their role as prognostic markers for this cancer. The cohort comprised 90 cases of the cervix lesions. The samples were assessed by immunohistochemistry for the expression of Bcl-2 and p53 proteins. The results showed that the Bcl-2 expression was either absent, low or moderate respectively in 38.96%; 50.65% and 10.39% of SCC cases. However, it was absent or expressed in 76.92% and 23.08% of adenocarcinoma cases respectively. The p53 protein was absent or present respectively in 75.32% and 24.68% of SCC cases as demonstrated by immunohistochemistry. p53 was almost absent in adenocarcinoma samples where only 7.70% of cases were positive. There was no significant correlation between Bcl-2 and p53 expression (p=0.352). We conclude that p53 expression, detected by immunohistochemistry, does not appear to be a prognostic marker for cervical cancer. Nevertheless, Bcl-2 expression seems to provide more information for this disease. It may represent an important indicator for cervical cancer.

scholarly journals Clinicopathologic Aspects of Squamous Cell Carcinoma of the Uterine Cervix: Role of PTEN, BCL2 and P53

2018 ◽  
Vol 8 (11) ◽  
pp. 2124 ◽  
Author(s):  
Ali Babiker ◽  
Ahmad Almatroudi ◽  
Khaled Allemailem ◽  
Nazik Husain ◽  
Mohamed Alsammani ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Uterine Cervix ◽  
P53 Protein ◽  
Clinical Stage ◽  
Pten Expression ◽  
Cancer Tissue ◽  
Inflammatory Lesions

Background: Squamous cell carcinoma (SCC) of the uterine cervix is a leading cause of morbidity and mortality among women. The alterations of Phosphatase and tensin homolog (PTEN), B-cell lymphoma 2 (Bcl2) and p53 expression seem to be significant in the development of various types of cancers. The altered expressions of PTEN, Bcl2 and p53 and their involvement in cancer of the uterine cervix are not well recognized. Aim: This study aimed at examining the expression patterns of PTEN, Bcl2 and p53 proteins and comparing them with the grade and stage of cervical cancer. Materials and Methods: Tissue blocks of SCC and ten cases of inflammatory lesions of the uterine cervix were examined immunohistochemically for the expression of PTEN, Bcl2 and p53 proteins. Results: Loss of PTEN expression was identified in 45.33% of cervical SCC and no expression was found in inflammatory lesions (p ≤ 0.05). PTEN expression was significantly associated with the clinical stage of SCC (61.36% and 45.16% in stages I–II and III–IV, respectively) (p < 0.05), but not with the degree of differentiation of the SCC. The expression of Bcl2 was significantly high (60%) in cancer cases than in control cases (p < 0.05). Bcl2 did not show any significant association with the histologic type and clinical stage of the SCC of the uterine cervix. The expression of p53 protein was significantly high (57.33%)) in cancer tissue, and no expression was noted in control cases (p < 0.05). Moreover, the expression pattern of p53 protein in cervical cancer tissue samples was not linked with the patient age, grade and stage of the cervical SCC (p > 0.05). Conclusion: The reduced expression of PETN and overexpressions of Bcl2 and p53 might play an indispensable role in carcinogenesis of cervical SCC. Moreover, a relationship was detected between PTEN expression and clinical stage of the cervical SCC.

scholarly journals p53 immunohistochemical staining patterns in oral squamous cell carcinoma

2016 ◽  
Vol 6 (12) ◽  
pp. 1013-1017
Author(s):  
G Dundy ◽  
H Kumar ◽  
A Singh ◽  
A Chandarakant
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Oral Mucosa ◽  
Squamous Cell ◽  
P53 Protein ◽  
P Value ◽  
P53 Expression ◽  
Normal Oral Mucosa ◽  
Significant Difference

Background: Mutation of p53 gene is one of the most common events in oral carcinogenesis. Accumulation of p53 protein has also been detected in premalignant lesions.Materials and Methods:  This study included 40 biopsy samples, which were received in department of pathology, Sarojini Naidu Medical College, Agra, to ascertain p53 expression by immunohistochemically, in patients with oral squamous cell carcinomas and to correlate its expression with histological grade, different sites in oral cavity and tobacco intake/smoking habits.Results: Out of 40 biopsies of oral mucosa, 03 showed normal oral mucosa and 37 were diagnosed as squamous cell carcinoma (SCC), most patients were in 5th and 6th decade and majority (86.5%) of oral SCC were males with buccal mucosa being the most common site. There was a statistically significant difference in p53 expression between oral SCC and normal oral mucosa (p value <0.05). Of total 37 cases, 12 cases were well differentiated type, 16 moderately differentiated and 09 of poorly differentiated type of SCC. In each category, about two thirds were positive for p53 staining. Out of total 37 cases of oral SCC, 64.9% were positive and 35.1% were negative for p53 expression, 34 cases had positive history of tobacco intake/smoking habits, of which 23 cases were positive while 11 cases were negative for p53 staining.Conclusion: Abnormal p53 protein was detected in 64.9% of oral squamous cell carcinoma, but not in normal oral mucosa. p53 expression was associated with malignant transformation of oral mucosa. 

Changing Incidence of Invasive Adenocarcinoma of the Uterine Cervix in East Anglia

1997 ◽  
Vol 4 (1) ◽  
pp. 40-43 ◽  
Author(s):  
Diane Stockton ◽  
Pauline Cooper ◽  
R N Lonsdale
Keyword(s):  
Cervical Cancer ◽  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Birth Cohort ◽  
Squamous Cell ◽  
Uterine Cervix ◽  
Invasive Adenocarcinoma ◽  
East Anglia ◽  
The Mean ◽  
Age Shift

Objective— To determine trends in incidence of invasive adenocarcinoma of the uterine cervix in East Anglia. Methods— Cervical cancer incidence data for both squamous cell carcinomas and adenocarcinomas were obtained from the East Anglian Cancer Registry for the period 1971–94. Similar data were obtained for England and Wales. European age standardised rates (ASRs) were used for comparisons. Results— The mean incidence (ASR) of cervical adenocarcinoma was 0.85 per 10s in 1971–76, rising to 2.54 per 105 in 1989–94. There has been a marked age shift, with the main increase in incidence occurring in younger women aged 30–39. The mean incidence (ASR) of squamous cell carcinoma of the cervix has decreased from 9.78 to 8.74 per 10s over the periods 1971–76 and 1989–94. Again there has been an age shift, moving from a single incidence peak in the 4S-S9 age band in earlier years to incidence peaks in both the 30–39 and 55–69 age bands in more recent years. Similar trends were noted when data for England and Wales were analysed. Birth cohort analyses show that both tumours are occurring progressively earlier (about five years earlier in each five year birth cohort). Conclusion— Although the overall incidence of cervical carcinoma is declining, this study has shown an increased incidence of cervical adenocarcinoma, particularly in the younger age groups. In future it would seem advisable to publish separate incidence and mortality data for squamous cell carcinoma and adenocarcinoma of the uterine cervix. All practitioners involved in the cervical cancer screening programme would then be aware of the very real significance of this tumour.

P53 expression as a predictor of recurrence in cervical squamous cell carcinoma

2002 ◽  
Vol 12 (3) ◽  
pp. 299-303 ◽  
Author(s):  
S. M. F Brenna ◽  
L. C Zeferino ◽  
G. A Pinto ◽  
R. A Souza ◽  
L. A. L Andrade ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Protein Expression ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
P53 Protein ◽  
Figo Stage ◽  
Cervical Squamous Cell Carcinoma ◽  
Stage Ii ◽  
P53 Expression ◽  
P53 Protein Expression

Abstract.Brenna SMF, Zeferino LC, Pinto GA, Souza RA, Andrade LAL, Vassalo J, Martinez EZ, Syrjänen KJ. P53 expression as a predictor of recurrence in cervical squamous cell carcinoma.P53 protein function is frequently down-regulated in cervical cancer by complexing with human papillomavirus (HPV) E6 protein, leading to degradation of p53, genomic instability, and mutations. Results are controversial, however, on the prognostic value of p53 protein expression in cervical cancer. In this study, a cohort of 220 Brazilian women with FIGO stage IB-III cervical squamous cell carcinoma (SCC), followed for 5 years, was analyzed for p53 protein expression using immunohistochemistry. The disease-free survival (DFS) and relapse rate were analyzed using univariate (Kaplan-Meier) and multivariable (Cox's proportional hazards model) survival analyses. P53 protein expression was detected in 35% of the patients, including 21% in stage I, 28% in stage II and 51% in stage III of disease. Of 220 women, only 116 completed one of the treatment options standardized by FIGO within 120 days. There was a higher risk of relapse in stage II and III disease, that was not modified by p53 positivity; HR 3.0 (1.3–6.5) to stage II and HR 4.0 (1.9–8.5) to stage III. The multivariate analysis evidenced that p53 expression is not an independent factor exceeding the power of FIGO stage as the single most important determinant of the hazards for disease relapse.

Squamous Cell Carcinoma Antigen for Monitoring Cervical Cancer

1988 ◽  
Vol 3 (2) ◽  
pp. 87-94 ◽  
Author(s):  
P. Schmidt-Rhode ◽  
K.D. Schulz ◽  
G. Sturm ◽  
H. Häfner ◽  
H. Prinz ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Uterine Cervix ◽  
Primary Treatment ◽  
Pelvic Lymphadenectomy ◽  
Treatment Modalities ◽  
Early Rise ◽  
Scc Antigen

The tumour-associated antigen was determined in the plasma of patients with squamous cell carcinoma (SCC) of the uterine cervix by radioimmunoassay. Setting a limit of 2 ng/ml, levels were abnormal in 13.4% of healthy controls, in 14% of patients with carcinoma in situ and in 62% of patients with invasive cervical SCC. The incidence of elevated SCC antigen levels and the absolute antigen plasma concentration were dependent upon the tumour load, increasing significantly with advanced stage disease. Abnormal SCC antigen values in operable cervical cancer declined to normal within one week after radical hysterectomy with pelvic lymphadenectomy. In cases of radiotherapy antigen values took 4-6 weeks after the start of treatment to return to normal. The success of both treatment modalities was announced by an early rise in the SCC antigen in the initial phase of therapy, followed by normalisation. After successful primary treatment and a complete remission during further follow-up SCC antigen in plasma was only increased in 3.8% of the cases. Retrospective evaluations in ten patients with progressive disease showed the reappearance of abnormal SCC titers and further increase preceeding the clinically detectable relapse or progression, with a median interval of 8 weeks. The present study indicates that SCC antigen determination is not useful for the early diagnosis of cervical cancer, but it is a potential means for monitoring the efficacy of individual anticancer therapy of SCC of the uterine cervix and for detecting recurrent disease.

Lymph node metastasis and lymph vascular space invasion in microinvasive squamous cell carcinoma of the uterine cervix

2006 ◽  
Vol 16 (3) ◽  
pp. 1184-1187 ◽  
Author(s):  
K. B.M. Lee ◽  
J. M. Lee ◽  
C. Y. Park ◽  
K. B. Lee ◽  
H. Y. Cho ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Squamous Cell Carcinoma ◽  
Lymph Node ◽  
Lymph Node Metastasis ◽  
Cell Carcinoma ◽  
Uterine Cervix ◽  
Depth Of Invasion ◽  
Node Metastasis ◽  
Vascular Space ◽  
Vascular Space Invasion

The objective of this study was to determine whether the depth of invasion was related to lymph vascular space invasion (LVSI) and lymph node metastasis and whether there was a correlation between LVSI and lymph node metastasis in stage IA cervical cancer. The medical records, including surgical notes and pathologic reports, of 202 patients with microinvasive squamous cell carcinoma of the uterine cervix were reviewed retrospectively. There was a positive correlation between the depth of invasion and the LVSI, and the incidence of lymph node metastasis was slightly higher than those reported hitherto for stage IA1 cervical cancer, especially in the depth of invasion of 1–3 mm group. However, among four patients with lymph node metastasis, only two patients had positive LVSI. There was no definite correlation between LVSI and lymph node metastasis. LVSI could not identify the patients with high risk for lymph node metastasis.

Loss of p16 INK4 expression in invasive squamous cell carcinoma of the uterine cervix is an adverse prognostic marker

2006 ◽  
Vol 49 (5) ◽  
pp. 542-545 ◽  
Author(s):  
H Masoudi ◽  
D J Van Niekerk ◽  
C B Gilks ◽  
M Cheang ◽  
K Bilek ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Prognostic Marker ◽  
Squamous Cell ◽  
Uterine Cervix ◽  
Invasive Squamous Cell Carcinoma
Sign in / Sign up

Export Citation Format

Share Document