scholarly journals Bacterial Lipopolysaccharide Vigorously Activate JAK2/STAT3 Induced Rheumatoid Arthritis Collate with Complete Freund's Adjuvant in Experimental Rats

2017 ◽  
Vol 10 (4) ◽  
pp. 2071-2076
Author(s):  
Omnia Ahmed Mohamed Abd El-Gaphar ◽  
Amira Mourad Abo-youssef ◽  
Gouda Kamel Helal
Keyword(s):  
Rheumatoid Arthritis ◽  
Complete Freund’S Adjuvant ◽  
Bacterial Lipopolysaccharide ◽  
Freund’S Adjuvant ◽  
Complete Freund's Adjuvant ◽  
Freund's Adjuvant

scholarly journals Radiological Analysis of Hind Paw Joint in Murine Rheumatoid Arthritis Model Treated Prophylactically Or Therapeutically with Cassia fistula versus Naproxen

2020 ◽  
Vol 34 (4) ◽  
pp. 58-63
Author(s):  
Hassan Farooq ◽  
Mariyam Iftikhar Piracha ◽  
Muhammad Usman ◽  
Rabia Tariq ◽  
Saadia Shahzad Alam
Keyword(s):  
Rheumatoid Arthritis ◽  
Complete Freund’S Adjuvant ◽  
Male Rats ◽  
X Rays ◽  
Cassia Fistula ◽  
Freund’S Adjuvant ◽  
Complete Freund's Adjuvant ◽  
Freund's Adjuvant ◽  
Animal House ◽  
Radiological Analysis

Introduction: Cassia fistula has anti-inflammatory activity. Rheumatoid arthritis (RA) is an autoimmune disease, causing inflammation and disability of the affected joints. It is mostly treated symptomatically with NSAIDs, which themselves have a lot of adverse effects. In this study the anti-arthritic effect of Cassia fistula versus naproxen has been observed radiologically. Aims & Objectives: The present study was radiological analysis of anti-arthritic effect of Cassia fistula compared to naproxen in Complete Freund’s Adjuvant (CFA) induced rat model of rheumatoid arthritis. Place and duration of study: This study was conducted at the Animal House of University of Veterinary and Animal Sciences, Lahore for a period of three months. Material & Methods: The study was carried out on 96 male rats divided into 12 groups of 8 rats each. Single 0.2ml dose of Complete Freund’s Adjuvant (CFA) was injected in the right hind paw of each rat in all groups except Group 1 (negative control). Group 2 was positive control. The prophylactic (3-7) and therapeutic (8- 12) groups were given naproxen (25mg/kg), anthraquinone extract (250 &500mg/kg) or methanolic extract (250 & 500mg/kg) of Cassia fistula orally BD on days 1,2 &3 (first dose preceding CFA injection by 30 minutes) and on days 9,10 &11 for therapeutic groups respectively. On day 15, x-rays of right ankle joints of all groups were taken as a confirmatory investigation on the final status of the arthritis. Results: Our results showed both anthraquinone and methanolic extracts of Cassia fistula have dose dependent (500˃250mg/dl) prophylactic and therapeutic anti-arthritic potential albeit lesser than naproxen 25mg/kg, to reduce the swelling of hind paw and deformity of interphalangeal joints on radiological examination. Conclusion: The efficacy of Cassia fistula makes it a potential weaker candidate to naproxen in prevention and treatment of rheumatoid arthritis.

scholarly journals THE POTENCY OF ALPHA LIPOIC ACID AS ANTI INFLAMMATORY ON THE COMPLETE FREUND'S ADJUVANT-INDUCED RHEUMATOID ARTHRITIS IN RAT MODEL

2017 ◽  
Vol 52 (2) ◽  
pp. 98
Author(s):  
Selvi Megawati ◽  
Mahardian Rahmadi ◽  
Imam Susilo ◽  
Junaidi Khotib
Keyword(s):  
Rheumatoid Arthritis ◽  
Lipoic Acid ◽  
Thermal Stimulation ◽  
Complete Freund’S Adjuvant ◽  
Alpha Lipoic Acid ◽  
Latency Time ◽  
Significant Difference ◽  
Freund’S Adjuvant ◽  
Complete Freund's Adjuvant ◽  
Freund's Adjuvant

Rheumatoid arthritis (RA) is an autoimmune diseases which is characterized by chronic inflammation of the synovial tissue in joints. This research was designed to investigate the effect of alpha lipoic acid as antioxidant on rats with complete freund’s adjuvant (CFA)-induced RA by intra articular injection of complete freund’s adjuvant (CFA). ALA was administered orally once a day for 7 days at 30, 60 and 120 mg doses a week after CFA injection. The severity of arthritis was evaluated by joint diameter and latency time on thermal stimulation. Joint diameter and latency time on thermal stimulation will measured on day 0, 3, 5, 7, 10, 12 and 14. Measurement of malondialdehyde (MDA) level in plasma was performed using thiobarbituric acid (TBA) method to assess lipid peroxidation. Histology of joint was examined by microscope following hematoxylin-eosin staining. The result showed that treatment with ALA at 30 mg and 60 mg significantly decreased the joint diameter compared to CFA group (p=0.003; p=0.001 respectively) and rat’s latency time on thermal stimulation was also significantly increased compared to CFA group (p=0.015; p=0.026 respectively). Measurement of MDA in CFA group and ALA group had no significant difference. Histological staining indicated that the recovery of the synovial membranes of joint in ALA group had no effect. Results indicated that ALA has the effect to suppress the development of inflammation in RA but not through oxidative stress pathway.

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