scholarly journals The Effects of Systemic Prescription of Amitriptyline on Orthodontic Tooth Movement Rate, Root Resorption and Alveolar Bone Remodeling in Dog

2017 ◽  
Vol 10 (3) ◽  
pp. 1537-1544
Author(s):  
Sara Siadat ◽  
Soosan Sadeghian ◽  
Majid Heidarpour ◽  
Sayed Mohammad Razavi ◽  
Seyed Sadra Izadi
Keyword(s):  
Bone Remodeling ◽  
Alveolar Bone ◽  
Tooth Movement ◽  
Root Resorption ◽  
Orthodontic Tooth Movement ◽  
Movement Rate ◽  
Alveolar Bone Remodeling ◽  
Tooth Movement Rate

Orthodontic Force–Induced BMAL1 in PDLCs Is a Vital Osteoclastic Activator

2021 ◽  
pp. 002203452110199
Author(s):  
Y. Xie ◽  
Q. Tang ◽  
S. Yu ◽  
W. Zheng ◽  
G. Chen ◽  
...  
Keyword(s):  
Bone Remodeling ◽  
Alveolar Bone ◽  
Tooth Movement ◽  
Orthodontic Tooth Movement ◽  
Nuclear Factor Κb ◽  
Periodontal Ligament Cells ◽  
Orthodontic Force ◽  
Periodontal Tissues ◽  
Alveolar Bone Remodeling ◽  
Biomechanical Stimuli

Orthodontic tooth movement (OTM) depends on periodontal ligament cells (PDLCs) sensing biomechanical stimuli and subsequently releasing signals to initiate alveolar bone remodeling. However, the mechanisms by which PDLCs sense biomechanical stimuli and affect osteoclastic activities are still unclear. This study demonstrates that the core circadian protein aryl hydrocarbon receptor nuclear translocator–like protein 1 (BMAL1) in PDLCs is highly involved in sensing and delivering biomechanical signals. Orthodontic force upregulates BMAL1 expression in periodontal tissues and cultured PDLCs in manners dependent on ERK (extracellular signal–regulated kinase) and AP1 (activator protein 1). Increased BMAL1 expression can enhance secretion of CCL2 (C-C motif chemokine 2) and RANKL (receptor activator of nuclear factor–κB ligand) in PDLCs, which subsequently promotes the recruitment of monocytes that differentiate into osteoclasts. The mechanistic delineation clarifies that AP1 induced by orthodontic force can directly interact with the BMAL1 promoter and activate gene transcription in PDLCs. Localized administration of the ERK phosphorylation inhibitor U0126 or the BMAL1 inhibitor GSK4112 suppressed ERK/AP1/BMAL1 signaling. These treatments dramatically reduced osteoclastic activity in the compression side of a rat orthodontic model, and the OTM rate was almost nonexistent. In summary, our results suggest that force-induced expression of BMAL1 in PDLCs is closely involved in controlling osteoclastic activities during OTM and plays a vital role in alveolar bone remodeling. It could be a useful therapeutic target for accelerating the OTM rate and controlling pathologic bone-remodeling activities.

Effects of sialoadenectomy and exogenous EGF on molar drift and orthodontic tooth movement in rats

1994 ◽  
Vol 266 (5) ◽  
pp. E731-E738 ◽  
Author(s):  
C. Dolce ◽  
J. Anguita ◽  
L. Brinkley ◽  
P. Karnam ◽  
M. Humphreys-Beher ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Remodeling ◽  
Alveolar Bone ◽  
Tooth Movement ◽  
Orthodontic Tooth Movement ◽  
Alveolar Bone Remodeling ◽  
Tooth Roots ◽  
Epidermal Growth ◽  
Tetracycline Labeling ◽  
Distal Aspect

Effects on bone remodeling have been attributed to epidermal growth factor (EGF). Sialoadenectomy (SX) removes the major source of EGF in rodents and decreases both salivary and serum EGF levels. EGF effects on rat alveolar bone remodeling manifested by molar drift (MD) and orthodontic tooth movement (OTM) were examined using the following two approaches: 1) EGF depletion by SX and replacement by orally administered EGF (50 micrograms.animal-1.day-1); 2) sham rats supplemented with matching amounts of EGF. MD and OTM were measured using cephalometric radiographs; bone formation was measured histomorphometrically using tetracycline labeling. Normal MD was not detected after SX, and alveolar bone formation was significantly reduced both around the tooth and in nondental sites. Replacement EGF given to SX rats and supplemental EGF administered to sham rats changed the direction and enhanced the rate of MD. A mesially directed orthodontic force applied to the molars of SX animals increased bone formation on the distal aspect of the tooth roots. Supplemental EGF did not significantly affect OTM. EGF affects alveolar bone remodeling, as manifested clinically by alterations in normal maxillary MD.

Locally Inhibition of Orthodontic Relapse by Injection of Carbonated Hydroxy Apatite-Advanced Platelet Rich Fibrin in a Rabbit Model

2017 ◽  
Vol 758 ◽  
pp. 255-263 ◽  
Author(s):  
Ananto Ali Alhasyimi ◽  
Pinandi Sri Pudyani ◽  
Widya Asmara ◽  
Ika Dewi Ana
Keyword(s):  
Bone Remodeling ◽  
Alveolar Bone ◽  
Tooth Movement ◽  
Rabbit Model ◽  
Orthodontic Tooth Movement ◽  
Potential Effect ◽  
Control Group ◽  
Hydroxy Apatite ◽  
Carbonated Apatite ◽  
Alveolar Bone Remodeling

Relapse is considered a significant failure after orthodontic treatment. In response to relapse, RANKL expressions will increase, while OPG expressions will decrease. CHA is thought to be one of an ideal candidate for enhancing bone formation. Moreover, a-PRF is a source high levels of growth factors that play a central role in the bone remodeling. This research was intended to investigate the effect of hydrogel CHA-aPRF in preventing relapse. Hydrogel-CHA was initially designed, with its degradation profile and FTIR (Fourie’s Transform Infrared) spectra were investigated as the basis to find out optimum formulation before incorporated with aPRF. Hydrogel-CHA microspheres were prepared in 3 different compositions: those were encoded 30-CHA, 40-CHA, and 50-CHA. After the hydrogel formulation and characterization were completed, 10 mL blood samples were collected, then centrifuged at 1500 rpm for 14 min. At the end of the centrifugation process, the aPRF clot was isolated and then pressed to obtain their releasate. The releasate aPRF was then loaded into the best formulation candidate of hydrogel CHA. The hydrogel incorporated aPRF was then gently injected on the mesial side of incisor gingival sulcus of the rabbit after orthodontic tooth movement. The FTIR analysis showed that carbonated apatite was successfully developed during the fabrication process of hydrogel-CHA microspheres. It was also known that degradation profile of 30-CHA was considered ideal compared to the other compositions. The application of CHA-aPRF (group C) was proven to significantly prevent relapse, indicated by lowest percentage of relapse 21 days after debonding (29.95±3.91%) compared to control group. Furthermore, it has been found that expressions of RANKL were significantly lowest (p<0.05) in group C on day 0, 3, and 7, while OPG expressions showed significantly highest (p<0.05) in group C on day 14 and 21 after debonding. These results indicate that incorporation of hydrogel-CHA has potential effect to enhance alveolar bone remodeling and prevent orthodontic relapse by stimulates OPG expression and suppresses RANKL expression.

scholarly journals ANALISIS VEGF PADA PERGERAKAN GIGI ORTODONTI SETELAH PEMBERIAN SEDUHAN KOPI ROBUSTA ( COFFEACANEPHORA)

2017 ◽  
Vol 5 (2) ◽  
pp. 90
Author(s):  
Herniyati Herniyati
Keyword(s):  
Bone Remodeling ◽  
Caffeic Acid ◽  
Alveolar Bone ◽  
Tooth Movement ◽  
Gingival Crevicular Fluid ◽  
Orthodontic Tooth Movement ◽  
Robusta Coffee ◽  
Alveolar Bone Remodeling ◽  
Group B ◽  
Compression Area

Background: Orthodontic tooth movement depends on bone remodeling. VEGF plays an important role in bone remodeling in both pressure area and tension area. Robusta coffee contains caffeine, chlorogenic acid and caffeic acid. Caffeine may increase osteoclastogenesis, and caffeic acid has antioxidant effects that may reduce oxidative stress in osteoblasts Objective: To analysis of VEGF of orthodontic tooth movement post robusta coffeesteeping administration. Material and methods: The experimental laboratories research used 16rats  were divided into 2 groups. Group A: the rats were applied with  orthodontic mechanical force (OMF) and group B: OMF + coffee steeping of 20mg /100 g of BW. OMF was conducted by applying  ligature wire with a diameter of 0.20 mm on the molar-1 (M-1) and both incivus of right maxilla. Subsequently, M-1 of right maxilla was moved to mesial  with Niti closed coil spring. Observations were made on days 15 and 22 by taking the gingival crevicular fluid by putting paper point on the gingival sulcus of mesio-and disto-palatal area of M-1 of right maxilla to determine the levels of VEGF using ELISA method. Results: the administration of Robusta coffee steeping increased the levels of VEGF in both compression area and tension area (p <0.05).The levels of VEGF in tension area larger than the compression area(p >0.05).Conclusion: the Robusta coffee steeping administration increased the levels of VEGF oforthodontic tooth movement ,therefore it may improve alveolar bone remodeling process and it may be an alternative to accelerate orthodontic treatment.

scholarly journals Mechanosensitive Piezo1 in Periodontal Ligament Cells Promotes Alveolar Bone Remodeling During Orthodontic Tooth Movement

2021 ◽  
Vol 12 ◽  
Author(s):  
Yukun Jiang ◽  
Yuzhe Guan ◽  
Yuanchen Lan ◽  
Shuo Chen ◽  
Tiancheng Li ◽  
...  
Keyword(s):  
Bone Remodeling ◽  
Periodontal Ligament ◽  
Alveolar Bone ◽  
Tooth Movement ◽  
Critical Role ◽  
Orthodontic Tooth Movement ◽  
Chemical Signals ◽  
Periodontal Tissues ◽  
Alveolar Bone Remodeling ◽  
Pdl Cells

Orthodontic tooth movement (OTM) is a process depending on the remodeling of periodontal tissues surrounding the roots. Orthodontic forces trigger the conversion of mechanical stimuli into intercellular chemical signals within periodontal ligament (PDL) cells, activating alveolar bone remodeling, and thereby, initiating OTM. Recently, the mechanosensitive ion channel Piezo1 has been found to play pivotal roles in the different types of human cells by transforming external physical stimuli into intercellular chemical signals. However, the function of Piezo1 during the mechanotransduction process of PDL cells has rarely been reported. Herein, we established a rat OTM model to study the potential role of Piezo1 during the mechanotransduction process of PDL cells and investigate its effects on the tension side of alveolar bone remodeling. A total of 60 male Sprague-Dawley rats were randomly assigned into three groups: the OTM + inhibitor (INH) group, the OTM group, and the control (CON) group. Nickel-titanium orthodontic springs were applied to trigger tooth movement. Mice were sacrificed on days 0, 3, 7, and 14 after orthodontic movement for the radiographic, histological, immunohistochemical, and molecular biological analyses. Our results revealed that the Piezo1 channel was activated by orthodontic force and mainly expressed in the PDL cells during the whole tooth movement period. The activation of the Piezo1 channel was essential for maintaining the rate of orthodontic tooth movement and facilitation of new alveolar bone formation on the tension side. Reduced osteogenesis-associated transcription factors such as Runt-related transcription factor 2 (RUNX2), Osterix (OSX), and receptor activator of nuclear factor-kappa B ligand (RANKL)/osteoprotegerin (OPG) ratio were examined when the function of Piezo1 was inhibited. In summary, Piezo1 plays a critical role in mediating both the osteogenesis and osteoclastic activities on the tension side during OTM.

The Effect of Amitriptyline and Sertraline on the Tooth Movement, Root Resorption and Alveolar Bone Remodeling After Load Application in Dogs

2021 ◽  
Author(s):  
Mehdi Rafiei ◽  
Sousan Sadeghian
Keyword(s):  
Bone Formation ◽  
Bone Remodeling ◽  
Alveolar Bone ◽  
Tooth Movement ◽  
Root Resorption ◽  
Load Application ◽  
Control Group ◽  
P Value ◽  
External Root Resorption ◽  
Alveolar Bone Remodeling

Introduction: Antidepressant drugs are the most commonly prescribed classes of pharmacologic agents in the public. The drugs have been shown to have a role in the regulation of bone cell function and as a result affecting the orthodontic tooth movements. The aim of this study was to determine the effect of Amitriptyline and Sertraline on the tooth movement, root resorption and alveolar bone remodeling after load application in dogs.   Materials and Methods: In this experimental study, 9 male dogs were randomly divided into three groups, first group sertraline, second group Amitriptyline and the control group (normal saline). A nickel titanium spring (200 gr) was used between second premolar and canine after 1st premolar extraction. After 2 months, the reduction of distance between 2nd premolar and canine was measured. The percentages of root resorption and bone formation were determined. The data were analyzed using repeated measures analysis at significance level of 0.05.   Results: In the three groups of amitriptyline, sertraline and control, the mean of teeth movement (p value = 0.483), external root resorption (p value = 0.608), total bone mineral density (p value = 0.078), bone formation percentage (p value = 0.616) and immature and lamellar bone formation (p value = 0.083), there was no statistical difference in any group and in premolar teeth Conclusions: The rate of tooth movement and the percentages of bone formation and root resorption in dogs decreased with systemic administration of amitriptyline and sertraline; although this reduction was not statistically significant in comparison with control group. 

FOXO3 Mediates Tooth Movement by Regulating Force-Induced Osteogenesis

2021 ◽  
pp. 002203452110215
Author(s):  
A. Jin ◽  
Y. Hong ◽  
Y. Yang ◽  
H. Xu ◽  
X. Huang ◽  
...  
Keyword(s):  
Bone Remodeling ◽  
Alveolar Bone ◽  
Tooth Movement ◽  
Orthodontic Tooth Movement ◽  
Osteoblastic Differentiation ◽  
Mechanical Force ◽  
Bone Mesenchymal Stem Cells ◽  
Efficient Manner ◽  
Alveolar Bone Remodeling

The high prevalence of malocclusion and dentofacial malformations means that the demand for orthodontic treatments has been increasing rapidly. As the biological basis of orthodontic treatment, the mechanism of mechanical force–induced alveolar bone remodeling during orthodontic tooth movement (OTM) has become the key scientific issue of orthodontics. It has been demonstrated that bone mesenchymal stem cells (BMSCs) are crucial for bone remodeling and exhibit mechanical sensing properties. Mechanical force can promote osteoblastic differentiation of BMSCs and osteogenesis, but the key factor that mediates mechanical force–induced osteogenesis during OTM remains unclear. In this study, by performing reverse-phase protein arrays on BMSCs exposed to mechanical force, we found that the expression level of forkhead box O3 (FOXO3) was significantly upregulated during the mechanical force–induced osteoblastic differentiation of BMSCs. The number of FOXO3-positive cells was consistently higher on the OTM side as compared with the control side and accompanied by the enhancement of osteogenesis. Remarkably, inhibiting FOXO3 with repaglinide delayed OTM by severely impairing mechanical force–induced bone formation in vivo. Moreover, knockdown of FOXO3 effectively inhibited the mechanical force–induced osteoblastic differentiation of BMSCs, whereas the overexpression of FOXO3 enhanced this effect. Mechanistically, we revealed a novel regulatory model in which FOXO3 promoted osteocalcin transcription by activating its promoter in cooperation with runt-related transcription factor 2 (RUNX2). We collectively obtained the first evidence that FOXO3 is critical for OTM, where it responds to mechanical force and directly regulates downstream osteoblastic differentiation in an efficient manner.

scholarly journals PENGARUH EKSTRAK KOPI ROBUSTA TERHADAP EKSPRESI OSTEOKALSIN PADA OSTEOBLAS SELAMA PERGERAKAN GIGI ORTODONTI

2017 ◽  
Vol 6 (1) ◽  
pp. 31
Author(s):  
Herniyati Herniyati
Keyword(s):  
Bone Remodeling ◽  
Alveolar Bone ◽  
Tooth Movement ◽  
Orthodontic Tooth Movement ◽  
Mechanical Force ◽  
Coil Spring ◽  
Immunohistochemical Examination ◽  
Robusta Coffee ◽  
Alveolar Bone Remodeling ◽  
Compression Area

The objective of  research is  to determine the expression of osteocalcin on osteoblasts during orthodontic tooth movement after administration robusta coffee extract. Materials and Methods: 16 rats  were divided into 2 groups: group K: rats applied with orthodontic mechanical force (OMF) and  group P: OMF +  coffee robusta extract of 20mg /100 g of BW. OMF was conducted by applying  ligature wire on permanent maxillary right first molar and both permanent maxillary incisivus. Subsequently, the permanent maxillary right first molar  moved to mesial with Ni-Ti orthodontic closed coil spring. Observations were made on day 15 by immunohistochemical examination to determine the expression of osteocalcin. Results: Robusta coffee extract  improved the expression of osteocalcin in the compression and tension areas (p <0.05). Expression of osteocalcin in  tension area  larger than that in compression area (p <0.05). Conclusion: The administration of Robusta coffee extract  increases the expression of osteocalcin, which can increase alveolar bone remodeling.

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