Molecular Fingerprinting of Indian Medicinal Tree Sara Asoca using RAPD Markers

2021 ◽  
Vol 17 (4) ◽  
pp. 701-705
Author(s):  
Shailendra Singh Yadav ◽  
Ashwini A. Waoo
Keyword(s):  
Cluster Analysis ◽  
Genomic Dna ◽  
Rapd Markers ◽  
Conservation Strategies ◽  
Molecular Fingerprinting ◽  
Initial Experiment ◽  
Medicinal Tree ◽  
Average Percentage ◽  
Saraca Asoca ◽  
Polymorphic Bands

Saraca asoca is an important medicinal tree facing a serious problem of reduction from its instinctivetenancy in India.Before formulation of conservation strategies for geographical protection of S. asoca genotypes available in India, it is necessary to characterize them. In the current study, the RAPD markers have been utilized effectively for categorization of S. asoca collected from 15 diverse sites of India. An initial experiment on the amplification suitability of genomic DNA samples of four S. asoca was done with 35RAPD primers. Among them only twenty sixproved their efficiency in two times repeat amplification.Total 146 bands were amplified and out of these 97 bands were found to be polymorphic. The average numbers of total band was 5.61 while average numbers of polymorphic bands was 3.73. The numbers of bands produced per primer ranged from 3 (OPE-15) to 8 (RUF205). Among all studied markers the highest percentage (100%) of polymorphism was demonstrated by only one marker (OPE-06). The lowest percent of polymorphicm (20%) was demonstrated by marker RUF211. The average percentage of polymorphism was 66.44%. Cluster analysis grouped all the S. asoca genotypes under study into two groups. Grouping of genotypes according to their sites of collection demonstrates higher similarity among or between them. The results obtained in the current study may help to formulate conservation strategies for the conservation of S. asoca genotypes.

scholarly journals Molecular characterization and genetic diversity of potato cultivars using SSR and RAPD markers

2010 ◽  
Vol 10 (3) ◽  
pp. 204-210 ◽  
Author(s):  
Elizângela Almeida Rocha ◽  
Luciano Vilela Paiva ◽  
Humberto Henrique de Carvalho ◽  
Claudia Teixeira Guimarães
Keyword(s):  
Genetic Diversity ◽  
Cluster Analysis ◽  
Ssr Markers ◽  
Genomic Dna ◽  
Rapd Markers ◽  
Potato Cultivars ◽  
High Information Content ◽  
Commercial Potato ◽  
Polymorphic Bands ◽  
High Information

This study aimed to evaluate the genetic diversity and identify potato cultivars by RAPD and SSR markers. The genomic DNA of 16 potato cultivars was amplified with 25 RAPD primers that generated 92 polymorphic bands and 20 SSR primer pairs that produced 136 polymorphic bands. The dendrograms generated by cluster analysis distinguished the cultivars genetically although the dendrograms were not correlated in the comparison of the two markers used. The PIC values demonstrated the high information content of the primers used and 16 potato varieties were identified based on six RAPD primers and three SSR primer pairs. Thus, by means of RAPD and SSR markers the genetic diversity was assessed and the 16 commercial potato cultivars analyzed in this study were identified.

scholarly journals 469 PB 336 INTER- AND INTRA-LINE GENOTYPIC VARIATION OF U. S. COLLARD CULTIVARS AND LANDRACES DETERMINED BY RAPD ANALYSIS

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 498d-498
Author(s):  
Mark W. Farnham
Keyword(s):  
Genetic Variation ◽  
Genomic Dna ◽  
Rapd Markers ◽  
Rapd Analysis ◽  
Genotypic Variation ◽  
Land Race ◽  
Commercial Cultivars ◽  
Polymorphic Bands ◽  
Line Analysis ◽  
Brassica Oleracea L

Collard (Brassica oleracea L. var. acephala) is an important vegetable the southeastern U. S. There are few (about 10) commercial cultivars, half being open-pollinating (OP) lines, the remainder more recent F1 hybrids. There is a potential untapped B. oleracea germplasm pool in the form of collard landraces perpetuated by southeastern gardeners and farmers. To determine the amount of genetic variation among cultivars and also whether landraces represent unique genotypes, ten cultivars and eight lines or landraces were evaluated using RAPD analysis. Decamer primers were used to amplify total genomic DNA and to differentiate collard lines and other B. oleracea crop cultivars. Additionally, individuals of an OP collard cultivar and a land-race were analyzed to evaluate intra-line variation. Virtually all primers detected polymorphic bands among lines although some identified considerably more variants. Intra-line analysis indicated that OP lines are genetically broad-based populations. Many unique RAPD markers were identified in landraces indicating that the lines represent unique genotypes and that further line collection is warranted.

scholarly journals Assessment of genetic diversity in bread wheat (Triticum aestivum L.) using RAPD markers

2017 ◽  
Vol 9 (3) ◽  
pp. 1751-1755
Author(s):  
Amit Kumar ◽  
R. S. Sengar ◽  
Vivekanand Pratap Rao ◽  
Gyanika Shukla ◽  
Rekha Dixit ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Cluster Analysis ◽  
Genomic Dna ◽  
Core Collection ◽  
Rapd Markers ◽  
Rapd Marker ◽  
Triticum Aestivum L ◽  
Wheat Cultivars ◽  
High Genetic Diversity ◽  
Wheat Genotypes

The present study aimed to evaluate the genetic diversity of 10 wheat cultivars by Random Amplified Pol-ymorphic DNA (RAPD) marker. The genomic DNA of 10 wheat genotypes were amplified with 10 RAPD primers that produced 53 amplified band, out of which 23 band were polymorphic (43.39%). The number of fragment amplified per primer ranged from 4 to 9. Primer A01 generated maximum number of amplified band, out of which 5 band were polymorphic. Cluster analysis of wheat genotypes were based on UPGMA method. Cluster analysis of 10 wheat genotypes were classified in to two main group; single variety AKW 1071 was placed in first group and rest 9 variety were placed in second group. The pair wise similarity values ranged from 0.58% to 100% and showed that cultivars Raj-3765 and K-7903 were the closest with highest similarity value (100%), while genotypes AKW 1071 and K9006 showed minimum similarity value (62%). The present study indicated the presence of high genetic diversity among wheat cultivars, which could be used for the developing core collection of wheat germplasm for breeding purpose.

Genetic diversity in Bambara groundnut (Vigna subterranea L.) germplasm revealed by RAPD markers

Genome ◽  
2001 ◽  
Vol 44 (6) ◽  
pp. 995-999 ◽  
Author(s):  
H I Amadou ◽  
P J Bebeli ◽  
P J Kaltsikes
Keyword(s):  
Genetic Diversity ◽  
Cluster Analysis ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Geographic Origin ◽  
Bambara Groundnut ◽  
International Institute ◽  
Tropical Agriculture ◽  
Vigna Subterranea ◽  
Ibadan Nigeria

Random amplified polymorphic DNA (RAPD) markers were used to assess genetic diversity in Bambara groundnut (Vigna subterranea L.) germplasm using 25 African accessions from the collection in the International Institute for Tropical Agriculture, Ibadan, Nigeria. Fifty random decamer primers were screened to assess their ability to detect polymorphism in bambara; 17 of them were selected for this study. Considerable genetic diversity was found among the V. subterranea accessions studied. The relationships among the 25 accessions were studied by cluster analysis. The dendrograms showed two main groups of accessions mainly along the lines of their geographic origin. It is concluded that RAPD can be used for germplasm classification in bambara groundnut and hence for improving this crop.Key words: germplasm, PCR, RAPD, Vigna subterranea.

Evaluation of Genetic Diversity in Castor (Ricinus communis L.) using RAPD Markes

2011 ◽  
Vol 343-344 ◽  
pp. 981-987
Author(s):  
Feng Juan Li ◽  
Chang Lu Wang ◽  
Dong He ◽  
Ya Qiong Liu ◽  
Mian Hua Chen ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Phylogenetic Tree ◽  
Genetic Distance ◽  
Oil Content ◽  
Rapd Markers ◽  
Rapd Analysis ◽  
Ricinus Communis ◽  
Major Group ◽  
Ricinus Communis L ◽  
Polymorphic Bands

RAPD markers are used to study the genetic diversity of the main planting on 37 castor varieties widely cultivated in china according to the oil content and other characteristic of different castor varieties. Genetic distance of 37 Chinese castor varieties is studied by RAPD markers analysis. RAPD analysis shows that a total of 122 bands are amplified from random primers of 20 S series, including 71 polymorphic bands with polymorphic rate of 58.20%. 37 castor beans are divided into four major groups in the phylogenetic tree. One castor germplasm is included in1, 2, 3 groups respectively, and two sub-groups are included in the 4 major group.

scholarly journals Genetic Diversity of Salvia Species Assessed by ISSR and RAPD Markers

2016 ◽  
Vol 44 (2) ◽  
pp. 431-436 ◽  
Author(s):  
Masoumeh YOUSEFIAZARKHANIAN ◽  
Ali ASGHARI ◽  
Jafar AHMADI ◽  
Behvar ASGHARI ◽  
Ali Ashraf JAFARI
Keyword(s):  
Genetic Diversity ◽  
Genetic Polymorphisms ◽  
Genomic Dna ◽  
Rapd Markers ◽  
Genetic Relationships ◽  
Genetic Variations ◽  
Molecular Techniques ◽  
Wild Populations ◽  
Similarity Coefficients ◽  
The World

The genus Salvia includes an enormous assemblage of nearly 1,000 species dispersed around the world. Due to possible threats to this genus, there is an immediate requirement to evaluate the diversity of its wild populations. ISSR and RAPD molecular techniques were used to evaluate the genetic relationships among twenty-one ecotypes of eight Salvia species. Amplification of genomic DNA using 23 primers (15 RAPD and eight ISSR) produced 280 bands, of which 91% were polymorphic. The results of marker parameters showed no clear difference between two marker systems. It was generally observed that both ISSR and RAPD markers had similar efficiency in detecting genetic polymorphisms with remarkable ability to differentiate the closely related ecotypes of Salvia. Nei’s similarity coefficients for these techniques ranged from 0.48 to 0.98. Based on the results of clustering, PCoA and AMOVA, the genetic diversity between and within species was confirmed. So, conservation and domestication of the genus Salvia must be due to levels of genetic variations.

scholarly journals Genetic variability and relationship among different accessions of Froriepia subpinata Bail (Gijavash) an endangered medicinal plant from Iran revealed by ISSR and IRAP markers

2021 ◽  
Vol 35 (1) ◽  
Author(s):  
Abbas Jorkesh ◽  
Yousef Hamidoghli ◽  
Jamalali Olfati ◽  
Habibollah Samizadeh ◽  
Davood Bakhshi
Keyword(s):  
Genetic Diversity ◽  
Cluster Analysis ◽  
Genetic Variability ◽  
Medicinal Plant ◽  
Genetic Information ◽  
Conservation Strategies ◽  
Polymorphic Band ◽  
Mean Values ◽  
Endangered Medicinal Plant ◽  
Irap Markers

The genetic variability of Froriepia subpinata Ledeb. Bail., an endangered Iranian endemic species, has been estimated with a total of 52 accessions using 20 markers including ISSR and IRAP. The results showed the polymorphic band produced by primers was 82.3%. The best mean values of genetic diversity parameters observed in ISSRs markers, being UBC873, UBC811, and UBC873 the best primers tested. The similarity range among accessions was 34.45% to 93.3%. The cluster analysis classified the accessions into five main groups that in totally, accessions with similarity in region generally were clustered in the same group. Overall, present study could provide elementary information for formulation of conservation strategies and invaluable elementary genetic information for next breeding or designing conservation programs.

scholarly journals Prediksi Resistensi Udang Vaname (Litopenaus vannamei) terhadap Infectious Hypodermal and Hematopoietic Necrosis Virus (IHHNV) dari Tambak Intensif dan Semi Intensif Jepara Menggunakan Marka RAPD

2013 ◽  
Vol 15 (2) ◽  
pp. 73
Author(s):  
Muhammad Mulyadi ◽  
Christina Ratna Handayani ◽  
Hermin Pancasakti Kusumaningrum ◽  
Anto Budiharjo
Keyword(s):  
Key Words ◽  
Rapd Markers ◽  
Infectious Virus ◽  
Necrosis Virus ◽  
Dna Virus ◽  
Polymorphic Bands ◽  
Dna Template

Infectious Hypodermal and Hematopoietic Necrosis Infectious Virus (IHHNV) is the most important DNA virus which can lead to Runt Deformity Syndrome (RDS) in vaname shrimp. The RAPD technique can be used to determine the resistance of a species to a disease. This research aimed to screen and identify RAPD markers which could distinguish the resistance of vaname shrimp to IHHNV reared at intensive and semi-intensive pond. The DNA template was amplified by PCR using 5 primers : OPA 06, OPA 08, OPA 19, OPD-02 and OPZ-15. The results showed that only the primer OPA-19 and OPZ-15 were able to produce 100% polymorphic bands with sizes from 400-1700 bp as well as showing the resistance IHHNV in vaname shrimp. Based on these results, vaname shrimp which reared at the intensive pond were more resistant to IHHNV compared with the semi-intensive pond.   Key words: RAPD, shrimp vaname, IHHNV, intensive & semi-intensive pond  

scholarly journals Population genetic and phytochemical dataset of Saraca asoca: A traditionally important medicinal tree

Data in Brief ◽  
2019 ◽  
Vol 25 ◽  
pp. 104173
Author(s):  
Satisha Hegde ◽  
Sandeep Ramchandra Pai ◽  
Rasika M. Bhagwat ◽  
Archana Saini ◽  
Poonam Kanwar Rathore ◽  
...  
Keyword(s):  
Population Genetic ◽  
Medicinal Tree ◽  
Saraca Asoca

RAPD and Protein Analyses Revealed Polymorphism in Mutated Potato Cultivars

2013 ◽  
Vol 64 (2) ◽  
Author(s):  
Irshad Ahmad ◽  
Samiullah Khan ◽  
Muhammad Arshad Javed ◽  
Fahrul Zaman Huyop ◽  
Muhammad Tariq ◽  
...  
Keyword(s):  
Banding Pattern ◽  
Total Protein ◽  
Genomic Dna ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Potato Cultivars ◽  
Polymorphic Behavior ◽  
Mutant Lines

Genomic DNA of the mutant lines of the three potato cultivars, Cardinal, Diamant and Desiree, with respect to controls were isolated and analyzed for polymorphisms by using random amplified polymorphic DNA (RAPD) markers. Four 10 bp random fragment primers, S-13, S-18, S-19 and R-17 were studied and all of them gave the amplification of genomic DNA. All of the mutant lines gave different banding pattern against different primers with respect to control plants of the three varieties, and bands are present at 50 bp to 1500 bp. All these primers with specific banding pattern were unique in their polymorphic behavior. Different banding pattern of total protein contents were also observed by PAGE analysis of all the mutant lines as compared with the control plants. It is therefore suggested that RAPD and protein analyses would be important tools to detect the polymorphism in mutated lines of potato.

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