scholarly journals Modifying Supports Surfaces by Dairy Wastewater Conditioning Film and Relationship with Initial Bacterial Adhesion

2020 ◽  
Vol 17 (03) ◽  
pp. 525-533
Author(s):  
Taoufik Hakim ◽  
Souad Lekchiri ◽  
Mohamed El Amine Afilal ◽  
Mostafa Ellouali ◽  
Hafida Zahir ◽  
...  
Keyword(s):  
Bacterial Adhesion ◽  
Dairy Wastewater ◽  
Conditioning Film

scholarly journals Bond Strengthening in Oral Bacterial Adhesion to Salivary Conditioning Films

2008 ◽  
Vol 74 (17) ◽  
pp. 5511-5515 ◽  
Author(s):  
Henny C. van der Mei ◽  
Minie Rustema-Abbing ◽  
Joop de Vries ◽  
Henk J. Busscher
Keyword(s):  
Bacterial Adhesion ◽  
Biofilm Formation ◽  
Specific Interactions ◽  
Adhesion Forces ◽  
Bacterial Strains ◽  
Bacterial Interactions ◽  
Initial Adhesion ◽  
Conditioning Film ◽  
Interacting Surfaces ◽  
Conditioning Films

ABSTRACT Transition from reversible to irreversible bacterial adhesion is a highly relevant but poorly understood step in initial biofilm formation. We hypothesize that in oral biofilm formation, irreversible adhesion is caused by bond strengthening due to specific bacterial interactions with salivary conditioning films. Here, we compared the initial adhesion of six oral bacterial strains to salivary conditioning films with their adhesion to a bovine serum albumin (BSA) coating and related their adhesion to the strengthening of the binding forces measured with bacteria-coated atomic force microscopy cantilevers. All strains adhered in higher numbers to salivary conditioning films than to BSA coatings, and specific bacterial interactions with salivary conditioning films were accompanied by stronger initial adhesion forces. Bond strengthening occurred on a time scale of several tens of seconds and was slower for actinomyces than for streptococci. Nonspecific interactions between bacteria and BSA coatings strengthened twofold faster than their specific interactions with salivary conditioning films, likely because specific interactions require a closer approach of interacting surfaces with the removal of interfacial water and a more extensive rearrangement of surface structures. After bond strengthening, bacterial adhesion forces with a salivary conditioning film remained stronger than those with BSA coatings.

scholarly journals Electrical microcurrent to prevent conditioning film and bacterial adhesion to urological stents

2010 ◽  
Vol 39 (2) ◽  
pp. 81-88 ◽  
Author(s):  
Michael Gabi ◽  
Lukas Hefermehl ◽  
Danijela Lukic ◽  
Raphael Zahn ◽  
Janos Vörös ◽  
...  
Keyword(s):  
Bacterial Adhesion ◽  
Conditioning Film

scholarly journals Uropathogens Preferrentially Interact with Conditioning Film Components on the Surface of Indwelling Ureteral Stents Rather than Stent Material

Pathogens ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 764
Author(s):  
Kymora B Scotland ◽  
Sonia HY Kung ◽  
Ben H Chew ◽  
Dirk Lange
Keyword(s):  
Bacterial Adhesion ◽  
Biofilm Formation ◽  
Bacterial Species ◽  
Potential Mechanism ◽  
Double Staining ◽  
Ureteral Stents ◽  
Conditioning Film ◽  
And Staphylococcus Aureus ◽  
Stent Material ◽  
Stent Surface

Despite routine implementation in urology, indwelling ureteral stents pose as a nidus for infection. Conditioning film accumulates on stents, which prime pathogen adhesion, promoting infectious biofilm formation. However, the extent to which conditioning film components play a role in facilitating bacterial adhesion and biofilm formation remains largely unknown. Here, we examined the interaction of previously identified stent-bound conditioning film components (fibrinogen, uromodulin, and albumin) with bacterial uropathogens. Cytoscopically removed stents were incubated with common uropathogens (Escherichia coli, Enterococcus faecalis, and Staphylococcus aureus). Immunofluorescent double staining was performed to study the localization of uropathogens relative to stent-bound conditioning film proteins. Conditioning film components were identified on the external stent surface with some deposition in the inner lumen. Bacteria co-localized with fibrinogen, uromodulin, and albumin, suggesting a potential mechanism for stent-associated infections. Here, we determine strong co-localization between common uropathogenic bacterial species with prominent conditioning film components on ureteral stents. Further functional validation of interactions amongst these uropathogens and conditioning film proteins may enhance clinical management for stent-associated infections and development of improved stent technologies.

UP-03.062 Identification of Conditioning Film Components on Indwelling Ureteral Stents and their Role in Bacterial Adhesion

Urology ◽  
2011 ◽  
Vol 78 (3) ◽  
pp. S363
Author(s):  
D. Lange ◽  
C. Elwood ◽  
A. Crowe ◽  
R. Miyaoka ◽  
H. Adomat ◽  
...  
Keyword(s):  
Bacterial Adhesion ◽  
Ureteral Stents ◽  
Conditioning Film

scholarly journals Multiple linear regression analysis of bacterial deposition to polyurethane coatings after conditioning film formation in the marine environment

Microbiology ◽  
2004 ◽  
Vol 150 (6) ◽  
pp. 1779-1784 ◽  
Author(s):  
Dewi P. Bakker ◽  
Henk J. Busscher ◽  
Joyce van Zanten ◽  
Joop de Vries ◽  
Job W. Klijnstra ◽  
...  
Keyword(s):  
Regression Analysis ◽  
Linear Regression ◽  
Multiple Linear Regression ◽  
Bacterial Adhesion ◽  
Film Formation ◽  
Linear Regression Analysis ◽  
Multiple Linear Regression Analysis ◽  
Polyurethane Coatings ◽  
Conditioning Film ◽  
Conditioning Films

Many studies have shown relationships of substratum hydrophobicity, charge or roughness with bacterial adhesion, although bacterial adhesion is governed by interplay of different physico-chemical properties and multiple regression analysis would be more suitable to reveal mechanisms of bacterial adhesion. The formation of a conditioning film of organic compounds adsorbed from seawater affects the properties of substratum surfaces prior to bacterial adhesion, which is a complicating factor in studying the mechanism of bacterial adhesion. In this paper, the impact of conditioning films adsorbed from natural seawater to four polyurethane coatings with different hydrophobicity, elasticity and roughness was studied for three different marine bacterial strains in a multiple linear regression analysis. The water contact angle on hydrophobic coatings decreased on average by 8 degrees and increased on average by the same amount on hydrophilic coatings. These changes were accompanied by increased concentrations of oxygen and nitrogen on the surface as determined by X-ray photoelectron spectroscopy, indicative of adsorption of proteinaceous material. Furthermore, the mean surface roughness increased on average by 4 nm after conditioning film formation. Multiple linear regression analysis revealed that changes in deposition due to conditioning film formation of Marinobacter hydrocarbonoclasticus, Psychrobacter sp. SW5H and Halomonas pacifica in a stagnation-point flow chamber could be explained in a model comprising hydrophobicity and the prevalence of nitrogen-rich components on the surface for the most hydrophobic strain. For the two more hydrophilic strains, deposition was governed by a combination of surface roughness and hydrophobicity. Elasticity was not a factor in bacterial adhesion to conditioning films.

1446 IDENTIFICATION OF CONDITIONING FILM COMPONENTS ON INDWELLING URETERAL STENTS AND THEIR ROLE IN BACTERIAL ADHESION

2010 ◽  
Vol 183 (4S) ◽  
Author(s):  
Dirk Lange ◽  
Chelsea N. Elwood ◽  
Adam Crowe ◽  
Ricardo Miyaoka ◽  
Manoj Monga ◽  
...  
Keyword(s):  
Bacterial Adhesion ◽  
Ureteral Stents ◽  
Conditioning Film

scholarly journals Analysis of Bacterial Detachment from Substratum Surfaces by the Passage of Air-Liquid Interfaces

2001 ◽  
Vol 67 (6) ◽  
pp. 2531-2537 ◽  
Author(s):  
Cristina Gómez-Suárez ◽  
Henk J. Busscher ◽  
Henny C. van der Mei
Keyword(s):  
Bacterial Adhesion ◽  
Flow Chamber ◽  
Liquid Interface ◽  
Streptococcus Sobrinus ◽  
Bacterial Strains ◽  
Experimental Conditions ◽  
Air Bubble ◽  
Coated Glass ◽  
Conditioning Film ◽  
Air Liquid Interface

ABSTRACT A theoretical analysis of the detachment of bacteria adhering to substratum surfaces upon the passage of an air-liquid interface is given, together with experimental results for bacterial detachment in the absence and presence of a conditioning film on different substratum surfaces. Bacteria (Streptococcus sobrinus HG1025,Streptococcus oralis J22, Actinomyces naeslundii T14V-J1, Bacteroides fragilis 793E, andPseudomonas aeruginosa 974K) were first allowed to adhere to hydrophilic glass and hydrophobic dimethyldichlorosilane (DDS)-coated glass in a parallel-plate flow chamber until a density of 4 × 106 cells cm−2 was reached. ForS. sobrinus HG1025, S. oralis J22, and A. naeslundii T14V-J1, the conditioning film consisted of adsorbed salivary components, while for B. fragilis 793E andP. aeruginosa 974K, the film consisted of adsorbed human plasma components. Subsequently, air bubbles were passed through the flow chamber and the bacterial detachment percentages were measured. For some experimental conditions, like with P. aeruginosa974K adhering to DDS-coated glass and an air bubble moving at high velocity (i.e., 13.6 mm s−1), no bacteria detached upon passage of an air-liquid interface, while for others, detachment percentages between 80 and 90% were observed. The detachment percentage increased when the velocity of the passing air bubble decreased, regardless of the bacterial strain and substratum surface hydrophobicity involved. However, the variation in percentages of detachment by a passing air bubble depended greatly upon the strain and substratum surface involved. At low air bubble velocities the hydrophobicity of the substratum had no influence on the detachment, but at high air bubble velocities all bacterial strains were more efficiently detached from hydrophilic glass substrata. Furthermore, the presence of a conditioning film could either inhibit or stimulate detachment. The shape of the bacterial cell played a major role in detachment at high air bubble velocities, and spherical strains (i.e., streptococci) detached more efficiently than rod-shaped organisms. The present results demonstrate that methodologies to study bacterial adhesion which include contact with a moving air-liquid interface (i.e., rinsing and dipping) yield detachment of an unpredictable number of adhering microorganisms. Hence, results of studies based on such methodologies should be referred as “bacterial retention” rather than “bacterial adhesion”.

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