scholarly journals In Vitro Assessment of Chromium, Lead, Cadmium and Nickel Tolerance of B. Clausii, a Prospective Probiotic Microorganism for in Vivo Bioremediation

2020 ◽  
Vol 17 (2) ◽  
pp. 255-266
Author(s):  
Pragya Goya ◽  
Pranoti Belapurkar ◽  
Anand Kar
Keyword(s):  
Nickel Tolerance ◽  
In Vitro Assessment ◽  
Probiotic Microorganism

In vitro assessment of food-derived-glucose bioaccessibility and bioavailability in bicameral cell culture system

2020 ◽  
Vol 45 (5) ◽  
pp. 631-637
Author(s):  
Cansu Ozel-Tasci ◽  
Gozde Pilatin ◽  
Ozgur Edeer ◽  
Sukru Gulec
Keyword(s):  
Cell Culture ◽  
Culture System ◽  
Metabolic Diseases ◽  
Enzymatic Digestion ◽  
Cell Culture System ◽  
Culture Studies ◽  
Experimental Approaches ◽  
In Vitro Assessment

AbstractBackgroundFunctional foods can help prevent metabolic diseases, and it is essential to evaluate functional characteristics of foods through in vitro and in vivo experimental approaches.ObjectiveWe aimed to use the bicameral cell culture system combined with the in vitro digestion to evaluate glucose bioavailability.Materials and methodsCake, almond paste, and pudding were modified by adding fiber and replacing sugar with sweeteners and polyols. Digestion process was modeled in test tubes. Rat enterocyte cells (IEC-6) were grown in a bicameral cell culture system to mimic the physiological characteristics of the human intestine. The glucose bioaccessibility and cellular glucose efflux were measured by glucose oxidase assay.Results and discussionThe glucose bioaccessibilities of modified foods were significantly lower (cake: 2.6 fold, almond paste: 9.2 fold, pudding 2.8 fold) than the controls. Cellular glucose effluxes also decreased in the modified cake, almond paste, and pudding by 2.2, 4, and 2 fold respectively compared to their controls.ConclusionOur results suggest that combining in vitro enzymatic digestion with cell culture studies can be a practical way to test in vitro glucose bioaccessibility and bioavailability in functional food development.

An Automated Technique for in Vitro Assessment of the Susceptibility of Urinary Catheter Materials to Encrustation

1987 ◽  
Vol 16 (1) ◽  
pp. 37-41 ◽  
Author(s):  
A J Cox ◽  
D W L Hukins ◽  
K E Davies ◽  
J C Irlam ◽  
T M Sutton
Keyword(s):  
Chemical Composition ◽  
Urinary Catheter ◽  
Reaction Vessel ◽  
Indwelling Catheters ◽  
Artificial Urine ◽  
In Vitro Assessment ◽  
Infected Urine ◽  
Automated Technique

An automated technique has been developed for assessing the extent to which existing or potential materials for the construction of indwelling catheters become encrusted during exposure to infected urine. In this technique the enzyme urease is added to artificial urine containing albumin in a reaction vessel which contains the samples to be tested. Controlled replacement of reactants leads to appreciable formation of encrusting deposits which adhere firmly to the surface of the test samples. Deposits have the same chemical composition as those which encrust catheters in vivo.

In Vivo Retrieval of a Trapped Merci Embolectomy Device: Technical Case Report

2010 ◽  
Vol 67 (3) ◽  
pp. onsE304-onsE304 ◽  
Author(s):  
Ajeet Gordhan ◽  
John Soliman
Keyword(s):  
Vertebral Artery ◽  
Conformational Changes ◽  
Basilar Artery ◽  
Technical Note ◽  
Vessel Injury ◽  
Artery Segment ◽  
In Vitro Assessment ◽  
Artery Flow

Abstract BACKGROUND AND IMPORTANCE: This technical note describes a complication related to the use of the Merci embolectomy device not previously reported. The device can induce critical flow limitation within an accessed vessel because of a combination of vasospasm and anatomic conformational changes. Furthermore, this can limit the safe removal of the device from intracranial vasculature. We present a novel rescue technique that can be used to safely retrieve the entrapped Merci device without inciting localized vessel injury. CLINICAL PRESENTATION: A 51-year-old male with embolic occlusion of the distal basilar artery and dissection-related occlusion of the left cervical vertebral underwent mechanical thrombolysis. Flow-limiting vasospasm and/or anatomic conformational changes/ telescoping of the intracranial right vertebral artery segment was induced during deployment with subsequent entrapment of the device. Reclamation of the entrapped device was performed by initially removing the Merci microcatheter. The entrapped and fixated device was then resheathed into a 4F slip catheter within the intracranial vertebral artery. The Merci device and the slip catheter were then removed. Right vertebral and proximal basilar artery flow was reestablished after removal of the Merci device. Successful clot extraction was thereafter performed using a microsnare. CONCLUSION: In vitro assessment of the device has demonstrated its propensity to induce vasospasm. In vivo entrapment of the device has not been previously reported. Successful retrieval can be achieved if the Merci device becomes entrapped and fixated. This may be an important consideration as increased utilization of the device occurs.

In Vitro Assessment of in Vivo Damage to the Barrier Properties of Pig Skin Caused by a Complex Mixture

1993 ◽  
Vol 12 (3) ◽  
pp. 239-248 ◽  
Author(s):  
Barbara W. Kemppainen ◽  
Pramod Terse ◽  
M. S. Madhyastha ◽  
S. D. Lenz ◽  
W. G. Palmer ◽  
...  
Keyword(s):  
Complex Mixture ◽  
Barrier Properties ◽  
Pig Skin ◽  
In Vitro Assessment

In Vivo and In Vitro Assessment of Vancomycin-Induced Nephrotoxicity

1988 ◽  
pp. 129-136 ◽  
Author(s):  
W. R. Hewitt ◽  
P. J. Bugelski ◽  
A. C. Silver ◽  
A. Klinkner ◽  
D. G. Morgan
Keyword(s):  
In Vitro Assessment

scholarly journals In vivo and in vitro assessment of brain bioenergetics in aging rats

2010 ◽  
Vol 14 (11) ◽  
pp. 2667-2674 ◽  
Author(s):  
Ol’ga Vančová ◽  
Ladislav Bačiak ◽  
Svatava Kašparová ◽  
Jarmila Kucharská ◽  
Hector H. Palacios ◽  
...  
Keyword(s):  
Aging Rats ◽  
In Vitro Assessment

An in Vivo and in Vitro Assessment of Differentiated Neuroblastoma Cells as a Source of Donor Tissue for Transplantation

1987 ◽  
Vol 495 (1 Cell and Tiss) ◽  
pp. 606-622 ◽  
Author(s):  
JEFFREY H. KORDOWER ◽  
MARY F. D. NOTTER ◽  
HERMES H. YEH ◽  
DON M. GASH
Keyword(s):  
Neuroblastoma Cells ◽  
Donor Tissue ◽  
In Vitro Assessment

In Vivo and In Vitro Assessment of an Intraoral Dental Colorimeter

2010 ◽  
Vol 19 (4) ◽  
pp. 279-285 ◽  
Author(s):  
Lale Karaagaclioglu ◽  
Hakan Terzioglu ◽  
Burak Yilmaz ◽  
Bengul Yurdukoru
Keyword(s):  
In Vitro Assessment
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