scholarly journals Optimization of Conditions for the Production of Recombinant Cellulase by using E.COLI BL21 Codon Plus in Fermenter

2020 ◽  
Vol 17 (01) ◽  
pp. 173-190
Author(s):  
Maliha Nawaz ◽  
S. Zafar ◽  
Sameen Khan Shervani ◽  
Sidra Ray ◽  
Ummul Buneen ◽  
...  
Keyword(s):  
Recombinant Cellulase

Recombinant cellulase of Caulobacter crescentus: potential applications for biofuels and textile industries

Cellulose ◽  
2021 ◽  
Author(s):  
Larissa Bussler ◽  
Débora Jacomini ◽  
Juliana Moço Corrêa ◽  
Marina Kimiko Kadowaki ◽  
Alexandre Maller ◽  
...  
Keyword(s):  
Caulobacter Crescentus ◽  
Potential Applications ◽  
Textile Industries ◽  
Recombinant Cellulase

scholarly journals Production of recombinant cellulase enzyme from Pleurotus ostreatus (Jacq.) P. Kumm. (type NRRL-0366)

2011 ◽  
Vol 5 (10) ◽  
pp. 1197-1202 ◽  
Author(s):  
S Daba Ayman ◽  
A Youssef Ghada ◽  
S Kabeil Sanaa ◽  
E Hafez Elsayed
Keyword(s):  
Pleurotus Ostreatus ◽  
Cellulase Enzyme ◽  
Recombinant Cellulase

Recombinant Cellulase Accumulation in the Leaves of Mature, Vegetatively Propagated Transgenic Sugarcane

2014 ◽  
Vol 56 (9) ◽  
pp. 795-802 ◽  
Author(s):  
Mark D. Harrison ◽  
R. Jason Geijskes ◽  
Robyn Lloyd ◽  
Stacy Miles ◽  
Anthony Palupe ◽  
...  
Keyword(s):  
Transgenic Sugarcane ◽  
Recombinant Cellulase

Cropβ-glucanase activity limits the effectiveness of a recombinant cellulase used to supplement a barley-based feed for free-range broilers

2008 ◽  
Vol 49 (3) ◽  
pp. 347-359 ◽  
Author(s):  
P.I.P. Ponte ◽  
M.M.S. Lordelo ◽  
C.I.P.D. Guerreiro ◽  
M.C. Soares ◽  
J.L. Mourão ◽  
...  
Keyword(s):  
Glucanase Activity ◽  
Free Range ◽  
Recombinant Cellulase

scholarly journals Selection of purification condition for recombinant endoglucanase originated from goat rumen bacteria in Escherichia coli

2020 ◽  
Vol 42 (1) ◽  
Author(s):  
Nguyen Thi Quy ◽  
Nguyen Hong Duong ◽  
Dao Trong Khoa ◽  
Nguyen Khanh Hoang Viet ◽  
Nguyen Khanh Hai ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Phosphate Buffer ◽  
Economic Value ◽  
Metagenomic Data ◽  
Affinity Column ◽  
Clear Zone ◽  
His Tag ◽  
Recombinant Cellulase ◽  
Important Enzyme ◽  
Selection Of

Cellulase is an important enzyme that plays a role in cleaving β-1,4 glucoside on cellulose to release glucose, which is of economic value and can be applied in many different fields. The 1545 bp endoglucanase gene mined from goat rumen's bacterial metagenomic data was expressed in Escherichia coli Rosetta2. In this study, the recombinant endoglucanase was purified by his-tag affinity chromatography with differrent processes, such as using phosphate buffer with or without sodium cloride, pretreatment of samples with ammonium sulphate before supplying into affinity column, using various concentration of imidazole for washing... Finally the endoglucanse was sucessfully purified by his-tag affinity column using sodium chloride-free phosphate buffer of which 150 mM and 400 mM imidazole were used for washing and enzyme elution, respectively. The resulting enzyme showed its high purity of 99%. CMC plate assay confirmed that although less active than commercial cellulase (Sigma), the recombinant cellulase hydrolyzed CMC to form a clear zone (halo) around the well. The purified enzyme is capable of using as material for further analysis. 

Sign in / Sign up

Export Citation Format

Share Document