scholarly journals Lipolysis Activity of Aflatoxins and Ochratoxins Producing Fungal Strains Contaminating Fresh Cow Meat

2020 ◽  
Vol 17 (01) ◽  
pp. 163-172
Author(s):  
A. A. El-Shahir ◽  
M. A. Abdel-Sater ◽  
A. S. Yassein
Keyword(s):  
Fungal Strains ◽  
Cow Meat

Synthesis, photosensitization and antimicrobial activity evaluation of some novel Merocyanine dyes

2019 ◽  
Author(s):  
Chem Int
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Aspergillus Flavus ◽  
Elemental Analysis ◽  
Cyanine Dyes ◽  
Fungal Strains ◽  
Activity Evaluation ◽  
Visible Spectra ◽  
Merocyanine Dyes

Novel acyclic and cyclic merocyanine dyes derived from the nucleu of furo [(3,2-d) pyrazole; ( d 2 , 3 )imidazole]were prepared. The electronic visible absorptionspectra of all the synthesized new cyanine dyes were examined in 95% ethanolsolution to evaluate their photosensitization properties. Antibacterial andantifungal activities for some selected dyes were tested against various bacterialand fungal strains (Escherichia coli, Staphylococcus aureus, Aspergillus flavus andCandida albicans) to evaluate their antimicrobial activity. Structural identificationwas carried out via elemental analysis, visible spectra, IR and 1H NMRspectroscopic data.

Synthesis and Biological Activity of 3-(substitutedphenyl)-6-(4-methoxyphenyl)-7H-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazine: Part II

2020 ◽  
Vol 18 ◽  
Author(s):  
Niranjan Kaushik ◽  
Nitin Kumar ◽  
Anoop Kumar ◽  
Vikas Sharma
Keyword(s):  
Antifungal Activity ◽  
Opportunistic Infections ◽  
Fungal Infections ◽  
Mass Spectral Data ◽  
Triazole Derivatives ◽  
Mass Spectral ◽  
Fungal Strains ◽  
Antifungal Properties ◽  
Antifungal Potential ◽  
Spectral Data Analysis

Background: Fungal infections are opportunistic infections that become a serious problem to human health. Objective: Considering the antifungal potential of triazole nucleus, the study was carried out with the objective to synthesize some novel triazole derivatives with antifungal potential. Method: 1,2,4-triazole derivatives were synthesized via a two step reaction (reported earlier). The first step involves reaction of substituted benzoic acid with thiocarbohydrazide to form 4-amino-3-(substituted phenyl)-5-mercapto-1, 2, 4-triazole derivatives (1a-1k) while in second step, synthesized compounds (1a-1k) were then subsequently treated with substituted acetophenone to yield substituted (4-methoxyphenyl-7H-[1, 2, 4] triazolo [3, 4-b][1,3,4] thiadiazine derivatives (2a-2k). All synthesized compounds were characterized by IR, 1H NMR, and Mass spectral data analysis and were screened for their antifungal properties against different fungal strains i.e. Candida tropicalis (ATCC-13803, ATCC-20913), Candida albicans (ATCC-60193), Candida inconspicua (ATCC-16783) and Candida glabrata (ATCC-90030, ATCC-2001). Results: Compound 2d displayed better percentage inhibition (26.29%, 24.81%) than fluconazole (24.44%, 22.96%) against ATCC-16783, ATCC-2001 fungal strains respectively at 100µg/ml. Compound 2f also displayed better percentage inhibition (28.51%) against ATCC-90030 as compared to fluconazone (27.4%) at 200 µg/ml. Similarly, compounds 2e and 2j also exhibited better antifungal properties than fluconazole at 200µg/ml. Compound 2e was found most potent against ATCC13803 (30.37%) and ATCC-90030 (30.37%) fungal strains as compared to fluconazole (28.14%, 27.4%) at 200 µg/ml respectively whereas compound 2j exhibited better antifungal activity (28.51%) against ATCC-60193 than fluconazole (27.7%) at 200 µg/ml. Conclusion: The results were in accordance with our assertions for triazole derivatives, as all compounds displayed moderate to good antifungal activity.

scholarly journals Nanoencapsulated Essential Oils with Enhanced Antifungal Activity for Potential Application on Agri-Food, Material and Environmental Fields

Antibiotics ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 31
Author(s):  
Magdaléna Kapustová ◽  
Giuseppe Granata ◽  
Edoardo Napoli ◽  
Andrea Puškárová ◽  
Mária Bučková ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Winning Strategy ◽  
Colloidal Suspensions ◽  
Fungal Species ◽  
Natural Environments ◽  
Food Material ◽  
Minimum Fungicidal Concentration ◽  
Fungal Strains

Nanotechnology is a new frontier of this century that finds applications in various fields of science with important effects on our life and on the environment. Nanoencapsulation of bioactive compounds is a promising topic of nanotechnology. The excessive use of synthetic compounds with antifungal activity has led to the selection of resistant fungal species. In this context, the use of plant essential oils (EOs) with antifungal activity encapsulated in ecofriendly nanosystems could be a new and winning strategy to overcome the problem. We prepared nanoencapsules containing the essential oils of Origanum vulgare (OV) and Thymus capitatus (TC) by the nanoprecipitation method. The colloidal suspensions were characterized for size, polydispersity index (PDI), zeta potential, efficiency of encapsulation (EE) and loading capacity (LC). Finally, the essential oil nanosuspensions were assayed against a panel of fourteen fungal strains belonging to the Ascomycota and Basidiomycota phyla. Our results show that the nanosystems containing thyme and oregano essential oils were active against various fungal strains from natural environments and materials. In particular, the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) values were two to four times lower than the pure essential oils. The aqueous, ecofriendly essential oil nanosuspensions with broad-spectrum antifungal activity could be a valid alternative to synthetic products, finding interesting applications in the agri-food and environmental fields.

scholarly journals Entomopathogenic fungi as the microbial frontline against the alien Eucalyptus pest Gonipterus platensis in Brazil

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Carolina Jordan ◽  
Paula Leite dos Santos ◽  
Leiliane Rodrigues dos Santos Oliveira ◽  
Mauricio Magalhães Domingues ◽  
Bianca Cristina Costa Gêa ◽  
...  
Keyword(s):  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae ◽  
Natural Variation ◽  
Fungal Strains ◽  
Conidial Production ◽  
Laboratory Conditions ◽  
Virulent Strains ◽  
Eucalyptus Plantations ◽  
Infected Adult

AbstractThe eucalyptus snout beetle (ESB), Gonipterus platensis, is endemic to Australia but has become a major invasive, destructive pest of Brazilian eucalyptus plantations. Efforts to develop insecticides based on entomopathogenic fungi against ESB are limited by the lack of known virulent strains. We therefore explored the virulence of indigenous Brazilian strains of major entomopathogenic fungi—Beauveria spp. and Metarhizium anisopliae—against ESB adults. We found widely varying virulence and later capacities for conidial production on infected adult cadavers. Two strains stood out, B. bassiana IBCB-240 and M. anisopliae IBCB-364, as especially lethal for ESB adults under laboratory conditions, sporulated abundantly on infected insects, and also outperformed comparable strains used in commercial mycoinsecticides. Notably, B. bassiana IBCB-240 exhibited lower LT50 values at low inoculum levels (≤ 107 conidia mL−1) and smaller LC50 values than M. anisopliae IBCB-364. Taken together, this study emphasizes natural variation in virulence among indigenous Beauveria and Metarhizium strains against ESB adults and identifies fungal strains with superior lethality to existing commercialized strains for managing this eucalyptus pest in Brazil.

scholarly journals Diversity and Drug Resistance of Filamentous Fungi Isolated from the Fresh Raspberries

2021 ◽  
Author(s):  
Ewelina Farian ◽  
Angelina Wójcik-Fatla
Keyword(s):  
Drug Resistance ◽  
Food Safety ◽  
Human Health ◽  
Amphotericin B ◽  
Filamentous Fungi ◽  
Rubus Idaeus ◽  
Disc Diffusion ◽  
Potential Threat ◽  
Fungal Strains ◽  
Strip Method

AbstractFungi are one of the most widely distributed microorganisms in the environment, including food such as fruits, vegetables and other crops, posing a potential threat to food safety and human health. The aim of this study was to determine the diversity, intensity and drug resistance of potentially pathogenic filamentous fungi isolated from the fresh raspberries (Rubus idaeus L.). A total of 50 strains belonging to genera Fusarium, Cladosporium, Alternaria, Penicillium, Mucor, Rhizopus, Aspergillus and Acremonium were tested for drug resistance against 11 antifungals by disc diffusion and gradient strips methods. The average mycological contamination in the examined samples of raspberries amounted to 4.34 log CFU/g. The Cladosporium was isolated from all tested samples, followed by Alternaria and Fusarium with a frequency of 61% and 34%, respectively. The highest level of drug resistance was observed for Acremonium genera and Fusarium strains recorded a wide variation in drug resistance as revealed by susceptibility with amphotericin B and voriconzole with MICs ranged from 0.5–4 µg/ml and posaconazole with MICs ranging from 3–8 µg/ml. All fungal strains showed 100% resistance to caspofungin, fluconazole and flucytosine with both the methods, and 100% resistance to micafungin and anidulafungin in the gradient strip method.

scholarly journals Virus Infection of Aspergillus fumigatus Compromises the Fungus in Intermicrobial Competition

Viruses ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 686
Author(s):  
Hasan Nazik ◽  
Ioly Kotta-Loizou ◽  
Gabriele Sass ◽  
Robert H. A. Coutts ◽  
David A. Stevens
Keyword(s):  
Virus Infection ◽  
Aspergillus Fumigatus ◽  
Stress Responses ◽  
Fungal Pathogens ◽  
Clinical Settings ◽  
Virus Infections ◽  
Fungal Strains ◽  
Common Reference ◽  
Strain Virus ◽  
Free Strain

Aspergillus and Pseudomonas compete in nature, and are the commonest bacterial and fungal pathogens in some clinical settings, such as the cystic fibrosis lung. Virus infections of fungi occur naturally. Effects on fungal physiology need delineation. A common reference Aspergillus fumigatus strain, long studied in two (of many) laboratories, was found infected with the AfuPmV-1 virus. One isolate was cured of virus, producing a virus-free strain. Virus from the infected strain was purified and used to re-infect three subcultures of the virus-free fungus, producing six fungal strains, otherwise isogenic. They were studied in intermicrobial competition with Pseudomonasaeruginosa. Pseudomonas culture filtrates inhibited forming or preformed Aspergillus biofilm from infected strains to a greater extent, also seen when Pseudomonas volatiles were assayed on Aspergillus. Purified iron-chelating Pseudomonas molecules, known inhibitors of Aspergillus biofilm, reproduced these differences. Iron, a stimulus of Aspergillus, enhanced the virus-free fungus, compared to infected. All infected fungal strains behaved similarly in assays. We show an important consequence of virus infection, a weakening in intermicrobial competition. Viral infection may affect the outcome of bacterial–fungal competition in nature and patients. We suggest that this occurs via alteration in fungal stress responses, the mechanism best delineated here is a result of virus-induced altered Aspergillus iron metabolism.

scholarly journals Wheat bran fermented by mixed fungal strains improves the digestibility of crude fiber and may benefit the gut health without impacting the growth performance in weaned pigs

2021 ◽  
Vol 12 (7) ◽  
pp. 2962-2971
Author(s):  
Yuheng Luo ◽  
Jun He ◽  
Hua Li ◽  
Cong Lan ◽  
Jingyi Cai ◽  
...  
Keyword(s):  
Growth Performance ◽  
Wheat Bran ◽  
Nutrient Digestibility ◽  
Crude Fiber ◽  
Gut Health ◽  
Intestinal Health ◽  
Weaned Piglets ◽  
Weaned Pigs ◽  
Fungal Strains

This study was conducted to compare the effect of raw (WB) or mixed fungi-fermented wheat bran (FWB) on the growth, nutrient digestibility and intestinal health in weaned piglets.

Mycelia extracts of fungal strains isolated from Cordyceps sinensis differently enhance the function of RAW 264.7 macrophages

2013 ◽  
Vol 148 (3) ◽  
pp. 818-825 ◽  
Author(s):  
Lan-Zhen Meng ◽  
Bao-Qin Lin ◽  
Bo Wang ◽  
Kun Feng ◽  
De-Jun Hu ◽  
...  
Keyword(s):  
Cordyceps Sinensis ◽  
Raw 264.7 ◽  
Raw 264.7 Macrophages ◽  
Fungal Strains
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