scholarly journals Formulation of a Suitable Medium and its Optimization for Maximizing L-asparaginase Production from Endophytic Fungi Fusarium sp. LCJ273

2018 ◽  
Vol 15 (4) ◽  
pp. 887-898
Author(s):  
V. Antony Jenila ◽  
J. Joel Gnanadoss
Keyword(s):  
Medicinal Plant ◽  
Ammonium Sulphate ◽  
Endophytic Fungi ◽  
Wheat Bran ◽  
Submerged Fermentation ◽  
Fold Increase ◽  
Statistical Optimization ◽  
Experimental Designs ◽  
Nutritional Parameters ◽  
Fusarium Sp

Endophytic fungi Fusarium sp. LCJ273 capable of producing L-asparaginase was isolated from the medicinal plant Adhatoda vasica. The aim of the present study was to maximize L-asparaginase production by submerged fermentation through statistical optimization. L-Asparaginase production by Fusarium sp. LCJ273 was studied in five different media. Various nutritional parameters specifically carbon, nitrogen and inducers were optimized for enhancing the production of L-asparaginase. In addition, different statistical based experimental designs were also applied to increase the production of L-asparaginase by Fusarium sp. LCJ273. Dextrose, ammonium sulphate and wheat bran were found to be effective for growth and higher yield of L-asparaginase in Modified Czapek’s Dox Broth. Dextrose at a concentration of 3.0 g/L increased L-asparaginase production up to 9.18±0.9 U/mL, ammonium sulphate at the concentration of 20 g/L showed maximum L-asparaginase production up to 13.69±0.4 U/mL and wheat bran at 2.5 g/L yielded up to 14.24±0.5 U/mL. The maximum L-asparaginase production was observed by Fusarium sp. LCJ273 on 5th day. The study revealed that through optimization, a 2 fold increase in L-asparaginase could be achieved.

scholarly journals Isolation and identification of phylloplane and endophytic fungi from Sapindus mukorossi

2020 ◽  
Vol 26 (2) ◽  
Author(s):  
B.D. Varpe
Keyword(s):  
Endophytic Fungi ◽  
Terrestrial Ecosystem ◽  
Fungal Species ◽  
Penicillium Expansum ◽  
Isolation And Identification ◽  
Epicoccum Nigrum ◽  
Candida Sp ◽  
Fusarium Sp ◽  
The Rich ◽  
High Level

Phylloplane biodiversity and endophytic fungi is considered one of the rich origins of novel biological activity compounds and high-level structural variation on the leaf surface. Plant leaves surface is a diverse terrestrial ecosystem, including filamentous fungi. This study aims to study the isolation and enumeration of Sapindus mukorossi phylloplane and endophytic fungal diversity. The Sapindus mukorossi isolated 14 fungal species from 9 genera of phylloplane and endophytic fungi. Cladosporium herbarum, Penicillium expansum, Fusarium oxysporum, Fusarium sp., Alternaria alternate, Collectotrichum orbiculare, Torulla herbarium, Epicoccum nigrum and Candida sp. as a phylloplane fungi. Aspergillus niger, A. flavus, Epicoccum nigrum. Penicillum digitatum, Penicillum sp. were identified as endophytic fungi.

PURIFICATION AND CHARACTERISATION OF FIBRINOLYTIC ENZYMES FROM ENDOPHYTIC FUNGI AND LIGNOSUS RHINOCERUS

2016 ◽  
Vol 78 (6-5) ◽  
Author(s):  
Zainon Mohd Noor ◽  
Mohd Sidek Ahmad ◽  
Zaidah Zainal Ariffin
Keyword(s):  
Ion Exchange ◽  
Endophytic Fungi ◽  
Gel Filtration ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Fibrinolytic Enzymes ◽  
Molecular Weights ◽  
Ammonium Sulphate Precipitation ◽  
Fusarium Sp ◽  
Different Sources

Three enzymes FH3, S13 and LR1 from three different sources showed fibrinolytic activities. Two were from endophytic fungal cultures and one from the sclerotium of Lignosus rhinocerus mushroom (LR1). FH3, S13 cultures and LR1, the crude extract of the sclerotium were concentrated and purified by ammonium sulphate precipitation, ion-exchange chromatography and gel-filtration. The molecular weights of the FH3, S13 and LR1 purified enzymes were estimated to be approximately 34kDa, 34kDa and 10kDa, respectively. Maximum fibrinolytic activities were observed for FH3 at pH 7 and 30°C, S13 at pH 8 and 40°C and LR1 at pH 6 and 40°C.  In our earlier paper we identified FH3 as Fusarium sp. and S13 as Penicilium citrinum. 

Biocatalytic benefits of immobilized Fusarium sp. (GFC) lipase from solid state fermentation on free lipase from submerged fermentation

2020 ◽  
Vol 147 ◽  
pp. 112235 ◽  
Author(s):  
Bruno Henrique de Oliveira ◽  
Gilberto Victor Coradi ◽  
Pedro de Oliva-Neto ◽  
Valeria Marta Gomes do Nascimento
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Submerged Fermentation ◽  
Fusarium Sp ◽  
Free Lipase

Endophytic fungi from the medicinal plant Aloe dhufarensis Lavranos exhibit antagonistic potential against phytopathogenic fungi

2020 ◽  
Author(s):  
Fatma Khuseib Hamed Al-Rashdi ◽  
Abdullah Mohammed Al-Sadi ◽  
Bahja Z. Al-Riyamy ◽  
Sajeewa S.N. Maharachchikumbura ◽  
Jamal Nasser Al-Sabahi ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Endophytic Fungi ◽  
Phytopathogenic Fungi ◽  
Antagonistic Potential

Endophytic fungi from the medicinal plant Lippia sidoides Cham. and their antimicrobial activity

Symbiosis ◽  
2011 ◽  
Vol 53 (2) ◽  
pp. 89-95 ◽  
Author(s):  
Virginia Medeiros de Siqueira ◽  
Raphael Conti ◽  
Janete Magali de Araújo ◽  
Cristina Maria Souza-Motta
Keyword(s):  
Antimicrobial Activity ◽  
Medicinal Plant ◽  
Endophytic Fungi ◽  
Lippia Sidoides

Statistical Optimization of the production of protease from Bacillus sp. MSK-01 in submerged fermentation

2018 ◽  
Vol 23 (4) ◽  
Author(s):  
Kulwant Kaur ◽  
Sonica Sondhi ◽  
Palki Sahib Kaur
Keyword(s):  
Submerged Fermentation ◽  
Milk Powder ◽  
Skim Milk ◽  
Fold Increase ◽  
Skim Milk Powder ◽  
Protease Production ◽  
High Yield ◽  
Bacillus Sp ◽  
Increasing Demand ◽  
Optimized Conditions

Protease has been in increasing demand in industries due to its hydrolytic nature. In industries, high yield of enzyme is required to meet the industrial need at a relatively cheaper cost. In the present study, the protease from Bacillus sp. MSK-01 was produced in large quantity by submerged fermentation. Statistical techniques including Plackett-Burman and Response surface methodology are useful tools for optimizing many parameters at a time and are used for increasing the protease production from Bacillus sp. MSK-01. 19 different parameters were chosen, out of which 15 factors had positive effect on protease yield. Four maximum influencing factors were peptone, magnesium sulphate, skim milk powder and casein were chosen to further increase the protease yield. 397.3 IU ml-1 of enzyme yield was obtained under optimized conditions which lead to 198 fold increase in the yield of protease from unoptimized condition. 

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