scholarly journals Biological Chemistry of Extreme Thermophile “Caldicellulosiruptor saccharolyticus”- A Future Biofuel Producer

2017 ◽  
Vol 14 (3) ◽  
pp. 1075-1080
Author(s):  
G.Sai Vaishnavi ◽  
D. Muralidhara Rao
Keyword(s):  
Fossil Fuels ◽  
Thermophilic Bacterium ◽  
Biological Chemistry ◽  
Extreme Thermophile ◽  
Caldicellulosiruptor Saccharolyticus

ABSTRACT: The present paper deals with some of those highlighting biochemical capabilities, that lay foundation to exploit the organism ‘caldicellulosiruptor saccharolyticus’emphasizing for finding substitutes for fossil fuels as the organism bears all hallmarks of being adopted as a ‘future biofuel producer’. Belonging to the genus ‘Caldicellulosiruptor’, it is one of the most well studied thermophilic bacterium that possess unique biological chemistry in fermenting substrates very easily with its efficient metabolism.

Characterization of a recombinant β-glucosidase from the thermophilic bacterium Caldicellulosiruptor saccharolyticus

2009 ◽  
Vol 108 (1) ◽  
pp. 36-40 ◽  
Author(s):  
Mi-Ri Hong ◽  
Yeong-Su Kim ◽  
Chang-Su Park ◽  
Jung-Kul Lee ◽  
Yeong-Suk Kim ◽  
...  
Keyword(s):  
Thermophilic Bacterium ◽  
Caldicellulosiruptor Saccharolyticus

Yields from Glucose, Xylose, and Paper Sludge Hydrolysate During Hydrogen Production by the Extreme Thermophile Caldicellulosiruptor saccharolyticus

2004 ◽  
Vol 114 (1-3) ◽  
pp. 497-508 ◽  
Author(s):  
Zsófia Kádár ◽  
Truus de Vrije ◽  
Giel E. van Noorden ◽  
Miriam A. W. Budde ◽  
Zsolt Szengyel ◽  
...  
Keyword(s):  
Hydrogen Production ◽  
Paper Sludge ◽  
Extreme Thermophile ◽  
Caldicellulosiruptor Saccharolyticus

scholarly journals Hydrogenomics of the Extremely Thermophilic Bacterium Caldicellulosiruptor saccharolyticus

2008 ◽  
Vol 74 (21) ◽  
pp. 6720-6729 ◽  
Author(s):  
Harmen J. G. van de Werken ◽  
Marcel R. A. Verhaart ◽  
Amy L. VanFossen ◽  
Karin Willquist ◽  
Derrick L. Lewis ◽  
...  
Keyword(s):  
Abc Transporters ◽  
Thermophilic Bacterium ◽  
Pentose Phosphate ◽  
Sugar Uptake ◽  
Circular Chromosome ◽  
High Hydrogen ◽  
Caldicellulosiruptor Saccharolyticus ◽  
Biomass Feedstocks ◽  
Desirable Feature ◽  
Pentose Phosphate Pathways

ABSTRACT Caldicellulosiruptor saccharolyticus is an extremely thermophilic, gram-positive anaerobe which ferments cellulose-, hemicellulose- and pectin-containing biomass to acetate, CO2, and hydrogen. Its broad substrate range, high hydrogen-producing capacity, and ability to coutilize glucose and xylose make this bacterium an attractive candidate for microbial bioenergy production. Here, the complete genome sequence of C. saccharolyticus, consisting of a 2,970,275-bp circular chromosome encoding 2,679 predicted proteins, is described. Analysis of the genome revealed that C. saccharolyticus has an extensive polysaccharide-hydrolyzing capacity for cellulose, hemicellulose, pectin, and starch, coupled to a large number of ABC transporters for monomeric and oligomeric sugar uptake. The components of the Embden-Meyerhof and nonoxidative pentose phosphate pathways are all present; however, there is no evidence that an Entner-Doudoroff pathway is present. Catabolic pathways for a range of sugars, including rhamnose, fucose, arabinose, glucuronate, fructose, and galactose, were identified. These pathways lead to the production of NADH and reduced ferredoxin. NADH and reduced ferredoxin are subsequently used by two distinct hydrogenases to generate hydrogen. Whole-genome transcriptome analysis revealed that there is significant upregulation of the glycolytic pathway and an ABC-type sugar transporter during growth on glucose and xylose, indicating that C. saccharolyticus coferments these sugars unimpeded by glucose-based catabolite repression. The capacity to simultaneously process and utilize a range of carbohydrates associated with biomass feedstocks is a highly desirable feature of this lignocellulose-utilizing, biofuel-producing bacterium.

scholarly journals Carbohydrate Utilization Patterns for the Extremely Thermophilic Bacterium Caldicellulosiruptor saccharolyticus Reveal Broad Growth Substrate Preferences

2009 ◽  
Vol 75 (24) ◽  
pp. 7718-7724 ◽  
Author(s):  
Amy L. VanFossen ◽  
Marcel R. A. Verhaart ◽  
Servé M. W. Kengen ◽  
Robert M. Kelly
Keyword(s):  
Transcriptional Response ◽  
Thermophilic Bacterium ◽  
Open Reading Frames ◽  
Transport Systems ◽  
Response Analysis ◽  
Growth Substrate ◽  
Phosphotransferase System ◽  
Carbohydrate Utilization ◽  
Caldicellulosiruptor Saccharolyticus ◽  
Substrate Preferences

ABSTRACT Coutilization of hexoses and pentoses derived from lignocellulose is an attractive trait in microorganisms considered for consolidated biomass processing to biofuels. This issue was examined for the H2-producing, extremely thermophilic bacterium Caldicellulosiruptor saccharolyticus growing on individual monosaccharides (arabinose, fructose, galactose, glucose, mannose, and xylose), mixtures of these sugars, as well as on xylan and xylogluco-oligosacchrides. C. saccharolyticus grew at approximately the same rate (t d, ∼95 min) and to the same final cell density (1 × 108 to 3 × 108 cells/ml) on all sugars and sugar mixtures tested. In the monosaccharide mixture, although simultaneous consumption of all monosaccharides was observed, not all were utilized to the same extent (fructose > xylose/arabinose > mannose/glucose/galactose). Transcriptome contrasts for monosaccharide growth revealed minimal changes in some cases (e.g., 32 open reading frames [ORFs] changed ≥2-fold for glucose versus galactose), while substantial changes occurred for cases involving mannose (e.g., 353 ORFs changed ≥2-fold for glucose versus mannose). Evidence for catabolite repression was not noted for either growth on multisugar mixtures or the corresponding transcriptomes. Based on the whole-genome transcriptional response analysis and comparative genomics, carbohydrate specificities for transport systems could be proposed for most of the 24 putative carbohydrate ATP-binding cassette transporters and single phosphotransferase system identified in C. saccharolyticus. Although most transporter genes responded to individual monosaccharides and polysaccharides, the genes Csac_0692 to Csac_0694 were upregulated only in the monosaccharide mixture. The results presented here affirm the broad growth substrate preferences of C. saccharolyticus on carbohydrates representative of lignocellulosic biomass and suggest that this bacterium holds promise for biofuel applications.

Glycolytic pathway and hydrogen yield studies of the extreme thermophile Caldicellulosiruptor saccharolyticus

2007 ◽  
Vol 74 (6) ◽  
pp. 1358-1367 ◽  
Author(s):  
T. de Vrije ◽  
A. E. Mars ◽  
M. A. W. Budde ◽  
M. H. Lai ◽  
C. Dijkema ◽  
...  
Keyword(s):  
Glycolytic Pathway ◽  
Hydrogen Yield ◽  
Extreme Thermophile ◽  
Caldicellulosiruptor Saccharolyticus
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