scholarly journals Comparison of the Effect of Various Extraction Methods on the Phytochemical Composition and Antioxidant Activity of Thymelaea hirsuta L. aerial parts in Tunisia

2017 ◽  
Vol 14 (3) ◽  
pp. 997-1007 ◽  
Author(s):  
Maroua Yahyaoui ◽  
Nessrine Ghazouani ◽  
Ines Sifaoui ◽  
Manef Abderrabba
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging ◽  
Extraction Methods ◽  
Antioxidant Power ◽  
Aerial Parts ◽  
Ferric Reducing Antioxidant Power ◽  
Soxhlet Method ◽  
Thymelaea Hirsuta ◽  
Extraction Processes

The present study aims to evaluate the effect of extraction methods namely soxhlet and cold maceration on the quality of T. hirsuta extracts, collected from different geographic regions within Tunisia. Hexane, ethyl acetate and methanol were used as solvent for both extraction processes. Yields varied considerably (from 0.61 to 8.11 %) according to sampling region, organic solvent and extraction method. The various chemical contents extracts were estimated by colorimetric methods, revealing important amounts of polyphenols (from 29.37 ± 1.34 to 259.63 ± 3.17 mg GAE/g) and flavonoids (from 26.22 ± 6.06 to 163.64 ± 3.32 mg QE/g). The antioxidant activity was measured using the DPPH-radical scavenging, the ABTS-radical scavenging and the ferric reducing antioxidant power (FRAP) assays. The antioxidant analysis showed that the methanol extract obtained by both processes exhibited the uppermost capacity to scavenge free radicals. However, the cold maceration technique leads to the richest extract in phenolic compounds compared to soxhlet method.

scholarly journals Comparison of phenolic profiles and antioxidant activity of three Ornithogalum L. species

2018 ◽  
Vol 44 (3) ◽  
pp. 299-306
Author(s):  
Gülin Renda ◽  
Arzu Özel ◽  
Emine Akyüz Turumtay ◽  
Burak Barut ◽  
Büşra Korkmaz ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Protocatechuic Acid ◽  
Radical Scavenging ◽  
Coumaric Acid ◽  
Antioxidant Power ◽  
Aerial Parts ◽  
Phenolic Profiles ◽  
Ferric Reducing Antioxidant Power ◽  
First Time

Abstract Background Aboveground parts and bulbs of Ornithogalum species are consumed as food and used in traditional medicine in worldwide. Objective It is aimed to report the antioxidant capacity and phenolic compounds content of Ornithogalum sigmoideum, Ornithogalum orthophyllum and Ornithogalum oligophyllum for the first time. Materials and methods Antioxidant activity of the crude methanol extracts of the aerial parts and the bulbs of the species were determined with 1,1-diphenyl-2-picryl-hydrazyl, superoxide radical scavenging, ferrous ion-chelating effect, phosphomolybdenum-reducing antioxidant power and ferric-reducing antioxidant power assay. The ethylacetate, diethylether and water subextracts from leaf and flower were analyzed to quantify selected phenolic compounds by HPLC-UV. Results Among the six extracts, the methanol extract of the aerial parts of O. orthophyllum contained the highest amount of phenolic compounds (GAE, 11.0 mg/g extract). The aerial parts of O. orthophyllum showed higher DPPH and SOD activities than the other extracts with the SC50 values of 0.39±0.05 mg/mL and 0.44±0.08 mg/mL, respectively. Protocatechuic acid, p-hydroxybenzoic acid, vanillic acid and p-coumaric acid were the most abundant compounds at all the subextracts. Conclusion The antioxidant activity is found to be in accordance with the levels of phenolic content in the extracts.

scholarly journals Composition, antimicrobial and antioxidant activity of the extracts of Eryngium palmatum Pančić and Vis. (Apiaceae)

2014 ◽  
Vol 9 (2) ◽  
pp. 149-155 ◽  
Author(s):  
Mirjana Marčetić ◽  
Silvana Petrović ◽  
Marina Milenković ◽  
Marjan Niketić
Keyword(s):  
Antioxidant Activity ◽  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Chloroform Extract ◽  
Volatile Fraction ◽  
Scavenging Activity ◽  
Antioxidant Power ◽  
Aerial Parts ◽  
Antimicrobial And Antioxidant Activity

AbstractThe chemical composition, antimicrobial and antioxidant activity of Eryngium palmatum, an endemic plant species from the Balkan Peninsula, were investigated. The flavonoids apigenin (9.5±0.3 mg g−1) and apigenin 7-O-glucoside (2.4±0.1 mg g−1) were determined in a methanol extract of aerial parts using HPLC analysis. The methanol extract of roots contained catechin (5.0±0.1 mg g−1), epicatechin (2.9±0.1 mg g−1), chlorogenic acid (1.6±0.0 mg g−1), gallic acid (0.9±0.0 mg g−1) and rosmarinic acid (0.9±0.2 mg g−1). GC-FID and GCMS analysis of a chloroform extract of aerial parts showed that the main volatile constituents were falcarinol, linoleic acid, hexadecanoic acid and methyl linoleate (comprising 32.6%; 24.4%; 19.9; 13.2% of the volatile fraction, respectively), while octanoic acid, tetradecanol and dodecanol dominated in the chloroform extract of the roots (34.9%; 25.8%; 22.2% of the volatile fraction, respectively). Investigation of antimicrobial activity by broth microdilution showed that the methanol and chloroform extracts of aerial parts and roots exerted a significant effect (MIC 3.5–15.6 μg mL−1) against tested Gram-positive and Gram-negative bacteria. The methanol extracts of aerial parts or roots exerted moderate ferric reducing antioxidant power, DPPH radical scavenging activity and hydroxyl radical scavenging activity.

scholarly journals Comparative Evaluation of Argania spinosa and Olea europaea Leaf Phenolic Compounds and their Antioxidant Activity

Botanica ◽  
2020 ◽  
Vol 26 (1) ◽  
pp. 76-87
Author(s):  
Aziza Lfitat ◽  
Hind Zejli ◽  
Abdelkamel Bousselham ◽  
Yassine El Atki ◽  
Badiaa Lyoussi ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Olive Leaves ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power ◽  
Total Flavonoids Content

AbstractWe conducted this study to determine and compare the content of phenolic compounds and flavonoids in the argan and olive leaves as well as their antioxidant capacity in aqueous, methanolic, and ethyl acetate extracted fractions. In vitro antioxidant activity was evaluated in comparison with synthetic antioxidants by assessing DPPH• radical scavenging capacity, ferric reducing antioxidant power, scavenging ability by inhibiting the β-carotene/linoleic acid emulsion oxidation, and by the ABTS radical scavenging activity assay. Total phenolic content in argan samples ranged from 221.69 ± 2.07 to 1.32 ± 0.01 mg GAE/g DW and in olive samples from 144.61 ± 0.82 to 1.21 ± 0.02 mg GAE/g DW. Total flavonoids content in argan samples varied from 267.37 ± 1.12 to 25.48 ± 0.02 mg QE/g DW, while in olives from 96.06 ± 0.78 to 10.63 ± 0.05 mg QE/g DW. In vitro antioxidant studies strongly confirmed the antioxidant potency of argan and olive leaves and their richness in secondary metabolites that are effective in free radicals scavenging and metal chelating capacities, indicating their antioxidant power.

scholarly journals Antioxidant poteintial and activity of aerial parts of eight medicinal plants of Uttarakhand, India

2019 ◽  
Vol 48 (2) ◽  
pp. 265-270
Author(s):  
Shakuli Kashyap ◽  
PB Rao ◽  
P Mishra ◽  
Supriya
Keyword(s):  
Radical Scavenging ◽  
Total Phenolic ◽  
Enzymatic Antioxidants ◽  
Antioxidant Power ◽  
Mean Values ◽  
Aerial Parts ◽  
Trolox Equivalent ◽  
Ferric Reducing Antioxidant Power ◽  
Dpph Radical Scavenging ◽  
Gallic Acid Equivalent

The antioxidant potential of aerial parts of eight medicinal plant species were investigated by 1,1- diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, Ferrous ion-chelating (FCA) and ferric reducing antioxidant power assays in five different concentrations and the mean values ranged from 20.39 to 83.01%, 17.21 to 48.62% and 11.70 to 21.65 μg Trolox equivalent/mg extract, respectively. Total phenolic and flavonoid contents ranged from 4.83 to 10.46 μg, gallic acid equivalent/mg extract and 1.12 to 13.11 μg quercitin equivalent/mg extract. Enzymatic antioxidants (Unit/mg fresh weight): superoxide dismutase, catalase and peroxidase values ranged from 14.86 to 49.81, 60.00 to 141.33 and 0.25 to 0.60, respectively. Considering the overall assay M. Pudica can be considered as the potent candidate for displaying antioxidant activity among the experimental plants.

scholarly journals Antibacterial and Antioxidant Activities of Derriobtusone A Isolated fromLonchocarpus obtusus

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Mayron Alves Vasconcelos ◽  
Francisco Vassiliepe Sousa Arruda ◽  
Daniel Barroso de Alencar ◽  
Silvana Saker-Sampaio ◽  
Maria Rose Jane Ribeiro Albuquerque ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Pathogenic Bacteria ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Test Results ◽  
Dpph Radical ◽  
Antioxidant Power ◽  
E Coli ◽  
Planktonic Growth ◽  
Ferric Reducing Antioxidant Power

This study evaluated the effect of derriobtusone A, a flavonoid isolated fromLonchocarpus obtusus, on two important pathogenic bacteria,Staphylococcus aureusandEscherichia coli, as well as its antioxidant activity and toxicity. Planktonic growth assays were performed, and the inhibition of biofilm formation was evaluated. In addition, antioxidant activity was assessed by DPPH radical scavenging assay, ferrous ion chelating assay, ferric-reducing antioxidant power assay, andβ-carotene bleaching assay. Toxicity was evaluated by the brine shrimp lethality test. Results showed that derriobtusone A completely inhibited the planktonic growth ofS. aureusat 250 and 500 μg/mL; however, it did not have the same activity onE. coli. Derriobtusone A reduced the biomass and colony-forming unit (cfu) ofS. aureusbiofilm at concentrations of 250 and 500 μg/mL. In various concentrations, it reduced the biofilm biomass ofE. coli, and, in all concentrations, it weakly reduced the cfu. Derriobtusone A showed highly efficient antioxidant ability in scavenging DPPH radical and inhibitingβ-carotene oxidation. The compound showed no lethality toArtemiasp. nauplii. In conclusion, derriobtusone A may be an effective molecule againstS. aureusand its biofilm, as well as a potential antioxidant compound with no toxicity.

scholarly journals Antioxidant activity and polyphenol composition of Pistacia terebinthus fruit from Tessala (Western Algeria)

2021 ◽  
Vol 5 (11) ◽  
pp. 30-34
Author(s):  
Nazim Bellifa ◽  
Abdelhak Ismail Benhaddou ◽  
Houssem Eddine Ferkous ◽  
Mohammed Adil Selka ◽  
Houari Toumi ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Scavenging Effect ◽  
Coffee Substitute ◽  
Ferric Reducing Antioxidant Power ◽  
Pistacia Terebinthus

Background: Consumption of traditional herbal beverages has been generally increased in the last decades, Terebinth coffee, known as ‘‘menengic coffee’’ in Turkish, is one of the most consumed herbal coffees in Turkey, turpentine tree is one of the components of the Mediterranean bush, particularly in Algeria, known as Betoum el Kiffan is largely used as food and in traditional medicine. Aims: In this study, Total phenol, flavonoid content, and antioxidant activity of three extracts of Pistacia trebinthus fruit growing in Algeria was measured using radical scavenging activity tests and metal-related tests including, ferric-reducing antioxidant power (FRAP). The chemical composition profile of the fruits and the coffee brands was identified by thin-layer chromatography, the effects of roasting method of this fruit was rivaled also. Materials and Methods: The total phenolic content of the extracts was determined using the Folin-Ciocalteu method. All extracts of the terebinth fruits and coffee brands displayed a high DPPH scavenging effect. Results: The results of the ferric-reducing antioxidant power show that the reduction capacity is proportional to the increase in the concentration of the samples. All the extracts of the plant exhibit antioxidant activities lower than those of the reference product besides the infusion extract of the P. terebinthus roasted coffee, which is the most active with an optical density of 1.68 nm at a concentration of 400 μg/mL. The chromatography results show that the various extracts of Pistacia terebinthus fruit carry a large number of polyphenols, in particular the carboxylic acids phenols. Conclusions: The plant can be considered as a coffee substitute and opens up promising avenues for the food and pharmaceutical industry in Algeria. Keywords: Antioxidant, Pistacia terebinthus, Coffee, FRAP, polyphenol.

scholarly journals PHYTOCHEMICAL SCREENING AND ANTIOXIDANT ACTIVITY OF Diospyros lotus L. FRUITS GROWN IN TURKEY

2020 ◽  
Vol 19 (2) ◽  
pp. 49-55
Author(s):  
Zehra Tuğba Murathan
Keyword(s):  
Fatty Acids ◽  
Antioxidant Activity ◽  
Fatty Acid ◽  
Radical Scavenging ◽  
Sinapic Acid ◽  
Ascorbic Acid Content ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Diospyros Lotus ◽  
Ferric Reducing Antioxidant Power

The present study aimed to analyze total ascorbic acid content (TAC), total phenolic content (TPC), total flavonoid content (TFC), phenolic composition, fatty acid profiles, and antioxidant activity of Diospyros lotus L. fruits grown in Turkey. The TAC, TPC and TFC of D. lotus extracts were 13.9, 130.3, and 12.7 mg/100 g, respectively. Phenolic compounds, gallic acid, vanillic acid, caffeic acid, p-coumaric acid, trans-ferulic acid, sinapic acid, naringin, rutin trihydrate, resveratrol, ellagic acid, trans-cinnamic acid, and quercetin were identified in persimmon samples. The 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) – ABTS and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activities and ferric reducing antioxidant power (FRAP) of fruit extracts were found to be 556.3 µmol/g, 69.6%, and 52.4%, respectively. Fructose was identified as the major sugar (371.01 mg/g), while sucrose was not detected. A total of 17 different fatty acids were identified. Most abundant saturated fatty acid (SFA), monounsaturated fatty acid (MUFA), and polyunsaturated fatty acids (PUFA) were palmitic acid (19.66%), palmitoleic acid (13.28%), and linoleic (18.04%) and gamma-linolenic (11.66%) acids, respectively.

scholarly journals Antioxidant activity of blackberry (Rubus sp.) genotypes from the Southern Region of Brazil

2013 ◽  
Vol 31 (1) ◽  
Author(s):  
GABRIELA ELISA HIRSCH ◽  
MÁRCIA VIZZOTTO ◽  
ANA LUCIA ABOY ◽  
AMÉLIA TEREZINHA HENRIQUES ◽  
TATIANA EMANUELLI
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging ◽  
Thiobarbituric Acid ◽  
Thiobarbituric Acid Reactive Substances ◽  
Frap Assay ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power ◽  
Tbars Assay ◽  
Β Carotene ◽  
Phenolic Extracts

The antioxidant activity and bioactive compounds of different blackberry fruit genotypes from the major Brazilian producer region (three cultivars and four selections) were evaluated and compared to the Cherokee cultivar. Phenolic and anthocyanic extracts were obtained and evaluated for each fruit genotype. The phenolic extracts of selections 02/96 and 07/001 presented higher antioxidant activity than those of cultivars in most assays. This activity was partially correlated to the higher amount of total phenolics in these samples. Thus, the phenolic compounds are probably the major responsible for the antioxidant activity in the diphenyl-2-picrylhydrazyl radical scavenging assay (DPPH), ferric reducing antioxidant power (FRAP) assay and thiobarbituric acid reactive substances (TBARS) assay. Quercetin seems to be responsible for the antioxidant activity of blackberry phenolic extracts in the β-carotene bleaching assay. Concerning anthocyanic extracts, the selection 02/96 and Cherokee cultivar from harvest 2007 had higher antioxidant activity than the other genotypes in most assays. Anthocyanins appear to be the major responsible for the antioxidant activity of anthocyanic extracts in the DPPH and FRAP assays, although ascorbic acid also contributed to the DPPH antioxidant activity. Selection 02/96 appears to have higher antioxidant activity than the commercial cultivars cultivated in the southern Brazil and appears to be promising for nutritional and health purposes.

scholarly journals Influence of Packaging Material on Polyphenol Content and Antioxidant Activity in Some Commercial Beers

Processes ◽  
2021 ◽  
Vol 9 (4) ◽  
pp. 620
Author(s):  
Mihaela Mirela Bratu ◽  
Semaghiul Birghila ◽  
Antoanela Popescu ◽  
Bogdan-Stefan Negreanu-Pirjol ◽  
Marius Radu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Polyphenol Content ◽  
Total Polyphenol ◽  
Antioxidant Power ◽  
Phenolic Contents ◽  
Significant Difference ◽  
Ferric Reducing Antioxidant Power ◽  
Lager Beer

Using two methods (ferric reducing antioxidant power and radical scavenging activity), the total polyphenol content (Folin–Ciocalteu reagent) and polyphenol patterns (HPLC) in 10 commercial lager beer brands produced in Romania was determined. Samples bottled in glass, plastic and aluminium packages were analysed for each brand when available. Results have indicated considerable variations in the total and individual phenolic contents as well as antioxidant activity across beer brands. Gallic and feulic acids were the dominant phenolic compounds identified samples. The statistical analysis indicates a significant difference between the levels of antioxidant activity detected using DPPH and FRAP, for all three types of material considered. The statistical test ANOVA indicates that the polyphenol concentration is similar across all types of material.

scholarly journals Anti-hyperglycaemic and anti-oxidant activities of five Nigerian antidiabetic plants

2019 ◽  
Vol 37 (2) ◽  
pp. 71-84
Author(s):  
M. D. Ayoola ◽  
A. C. Adebajo ◽  
E. M. Obuotor ◽  
T. O. Oladapo ◽  
T. C. Fleischer
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Antioxidant Property ◽  
Diabetic Rats ◽  
Antioxidant Power ◽  
Glucose Levels ◽  
Methanolic Extracts ◽  
Normal Glucose ◽  
Ferric Reducing Antioxidant Power

Leaves of Olax subscorpioidea, Hoslundia opposita, Cleistopholis patens, Plumbago zeylanica and Dioscoreophyllum cumminsii that are used as anti-diabetics were evaluated for hyperglycaemic-lowering and antioxidant activities to justify their Nigerian ethnomedicinal usage. Leaf methanolic extracts (100, 200 and 400 mg/kg, p.o.) were assayed in normal, glucose- and alloxan-induced diabetic rats, while 1,1,-diphenyl-2-picrylhydrazyl, total antioxidant capacity, ferric reducing antioxidant power and hydroxyl radical scavenging tests were used for their antioxidant activity. Effects of n-hexane, dichloromethane, ethylacetate and aqueous partition fractions of the three most active anti-hyperglycaemic extracts were also tested in glucose-loaded rats. In normoglycaemic rats, all extracts generally lacked activity, while with glucose-loaded rats, only O. subscorpioidea (200mg/kg) and C. patens (400 mg/kg) at 1 h and O. subscorpioidea (200, 400 mg/kg) and C. patens (400 mg/kg) at 2-4 hours gave lowered (p < 0.05) blood glucose levels than glibenclamide (5 mg/kg), while activity of H. opposita and P. zeylanica (200 mg/kg) were only comparable to glibenclamide. When daily fed for fourteen days to alloxan-induced diabetic rats, all extracts, at their most active doses, gave significantly higher activity than glibenclamide. Olax subscorpioidea leaf extract had the highest hyperglycaemic-lowering and least antioxidant activities. Highest antioxidant activity of H. opposita may suggest some contribution of antioxidant property to its hyperglycaemic-lowering activity. The glucose-lowering and insulinotropic constituents of O. subscorpioidea, H. opposita and C. patens should be concentrated in their aqueous, aqueous and dichloromethane fractions, respectively. Anti-hyperglycaemic ethnomedicinal use of these plants was justified and H. opposita has additional antioxidant property. Keywords: Antioxidant activity; Diabetes mellitus; Nigerian anti-diabetic plants 

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