scholarly journals Innovative Optimal Approach to Implement Fast Propagation Tissue Culture

2017 ◽  
Vol 14 (2) ◽  
pp. 615-620
Author(s):  
Hossein Nazarian ◽  
Amir Hossein Ghadimi
Keyword(s):  
Tissue Culture ◽  
Plant Growth ◽  
Growth Regulators ◽  
Optimal Placement ◽  
Regeneration Ability ◽  
Adaxial Side ◽  
Placement Method ◽  
Optimal Approach ◽  
Different Parts ◽  
Better Than

ABSTRACT: In this study bulbetets of royal lily have been used as explants. The innovative approach is based on optimal placement and adventitious sprout induction and the adventitious sprout proliferation in special conditions. Then effects of different parts of scales and placement method, types and concentrations of cytokinin, different parts of bulbetet scales and placement method on adventitious sprout induction have been discussed. At the end the effects of plant growth regulators on induction and multiplication of bulbetet scales of royal lily have been studied. The results represents that the regeneration ability of outer layer scales of royal lily is better than the middle ones, which was better than the inner ones. The adaxial side of royal lily bulbetets in contact with medium upward can produce more and stronger adventitious sprouts than the adaxial side downward.

scholarly journals Application of Plant Extracts in Micropropagation and Cryopreservation of Bleeding Heart: An Ornamental-Medicinal Plant Species

Agriculture ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 542
Author(s):  
Dariusz Kulus ◽  
Natalia Miler
Keyword(s):  
Tissue Culture ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Plant Extracts ◽  
The Other ◽  
White Gold ◽  
Natural Extracts ◽  
Other Hand ◽  
The Impact

Lamprocapnos spectabilis (L.) Fukuhara (bleeding heart) is valued both in the horticultural and pharmaceutical markets. Despite its great popularity, information on the in vitro tissue culture technology in this species is limited. There is also little knowledge on the application of plant extracts in the tissue culture systems of plants other than orchids. The aim of this study is to compare the utility of traditional plant growth regulators (PGRs) and natural extracts—obtained from the coconut shreds, as well as oat, rice, and sesame seeds—in the micropropagation and cryopreservation of L. spectabilis ‘Gold Heart’ and ‘White Gold’. The biochemical analysis of extracts composition is also included. In the first experiment related to micropropagation via axillary buds activation, the single-node explants were cultured for a 10-week-long propagation cycle in the modified Murashige and Skoog medium fortified either with 1.11 µM benzyladenine (BA) and 1.23 µM indole-3-butritic acid (IBA) or with 10% (v/v) plant extracts. A PGRs- and extract-free control was also considered. In the cryopreservation experiment, the same 10% (v/v) extracts were added into the medium during a seven-day preculture in the encapsulation-vitrification cryopreservation protocol. It was found that the impact of natural additives was cultivar- and trait-specific. In the first experiment, the addition of coconut extract favoured the proliferation of shoots and propagation ratio in bleeding heart ‘Gold Heart’. Rice extract, on the other hand, promoted callus formation in ‘White Gold’ cultivar and was more effective in increasing the propagation ratio in this cultivar than the conventional plant growth regulators (4.1 and 2.6, respectively). Sesame extract suppressed the development of the explants in both cultivars analysed, probably due to the high content of polyphenols. As for the second experiment, the addition of plant extracts into the preculture medium did not increase the survival level of the cryopreserved shoot tips (sesame and oat extracts even decreased this parameter). On the other hand, coconut extract, abundant in simple sugars and endogenous cytokinins, stimulated a more intensive proliferation and growth of shoots after rewarming of samples. Analysing the synergistic effect of conventional plant growth regulators and natural extracts should be considered in future studies related to L. spectabilis.

scholarly journals The Effect of Plant Growth Regulators on Callus Induction and Regeneration of Amygdalus communis

2011 ◽  
Vol 3 (3) ◽  
pp. 97-100
Author(s):  
Naimeh SHARIFMOGHADAM ◽  
Abbas SAFARNEJAD ◽  
Sayed Mohammad TABATABAEI
Keyword(s):  
Tissue Culture ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Base Material ◽  
Culture Technique ◽  
Induction Medium ◽  
Root Induction ◽  
Amygdalus Communis

The Almond (Amygdalus communis) is one of the most important and oldest commercial nut crops, belonging to the Rosaceae family. Almond has been used as base material in pharmaceutical, cosmetic, hygienically and food industry. Propagation by tissue culture technique is the most important one in woody plants. In the current research, in vitro optimization of tissue culture and mass production of almond was investigated. In this idea, explants of actively growing shoots were collected and sterilized, then transferred to MS medium with different concentrations and combinations of plant growth regulators. The experiment was done in completely randomized blocks design, with 7 treatment and 30 replications. After 4 weeks, calli induction, proliferation, shoot length and number of shoot per explants were measured. Results showed that the best medium for shoot initiation and proliferation was MS + 0.5 mg/l IAA (Indol-3-Acetic Acid) + 1 mg/l BA (Benzyl Adenine). Autumn was the best season for collecting explants. The shoots were transferred to root induction medium with different concentrations of plant growth regulators. The best root induction medium was MS + 0.5 mg/l IBA (Indol Butyric Acid).

scholarly journals RESPONS NAPHTHALEN ACETIC ACID DAN UNSUR MIKRO MIKOMBI SUPER TERHADAP CHERELLE WILT PADA TANAMAN KAKAO

EUGENIA ◽  
2012 ◽  
Vol 21 (3) ◽  
Author(s):  
Stanley A.F. Walingkas ◽  
Meity Rantung
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Nutrient Elements ◽  
The Third ◽  
Significant Difference ◽  
Treatment Factor ◽  
The Right ◽  
Micro Elements ◽  
Better Than

ABSTRACT This study aims to determine the effect of plant growth regulators and micro elements to cherelle wilt in cacao plants as well as finding the right business in tackling cherelle wilt in cacao plants. The research was carried out in the garden village folk Eris Eris Minahasa district for 8 months from January to August 2009. Spraying micro-nutrient elements is necessary to reduce cherelle wilt because the various treatments for the first observation is not significantly different, but the observations into two to seven observations to indicate a significant difference between treatments (percentage cherelle wilt on treatment without nutrient elements higher than sprayed). While spraying the treatment factor of plant growth regulators (NAA) is not necessary because the first observation and the two had no significant effect on the percentage of cherelle wilt affected only the third observation and further observations to a fourth, fifth, sixth and seventh again no effect. So is the treatment for fruit length and diameter showed that administration of the micro nutrients necessary for sprayed with micro nutrients result 22.10 cm higher than that is not sprayed is 19.43 cm. As for the diameter of the fruit is affected by plant growth regulators. Treatment is better than 200 ppm 100 ppm and 0 ppm (control). Keywords: NAA, cocoa, mikombi     ABSTRAK   Penelitian ini bertujuan mengetahui pengaruh pemberian zat pengatur tumbuh dan unsur mikro terhadap cherelle wilt pada tanaman kakao serta menemukan usaha yang tepat dalam menanggulangi cherelle wilt pada tanaman kakao. Penelitian ini dilaksanakan di kebun rakyat Desa Eris Kecamatan Eris Kabupaten Minahasa selama 8 bulan sejak Januari – Agustus 2009. Penyemprotan unsur hara mikro perlu dilakukan untuk mengurangi cherelle wilt sebab pada berbagai perlakuan untuk pengamatan pertama tidak berbeda nyata namun pada pengamatan ke dua sampai pengamatan ke tujuh menunjukkan adanya perbedaan nyata antar perlakuan (prosentase cherelle wilt pada perlakuan tanpa unsure hara lebih tinggi dari yang disemprot). Sementara faktor penyemprotan dengan perlakuan zat pengatur tumbuh (NAA) tidak perlu dilakukan sebab pengamatan pertama dan ke dua tidak berpengaruh nyata pada persentase cherelle wilt hanya berpengaruh pada pengamatan ke tiga dan selanjutnya pengamatan ke empat, ke lima, ke enam dan ke tujuh kembali tidak berpengaruh. Begitu juga perlakuan untuk panjang dan diameter buah menunjukkan bahwa pemberian unsur hara mikro perlu dilakukan sebab yang disemprot dengan unsur hara mikro hasilnya 22,10 cm lebih tinggi dari yang tidak disemprot yaitu 19,43 cm. Sementara untuk diameter buah dipengaruhi oleh zat pengatur tumbuh. Perlakuan 200 ppm lebih baik dari 100 ppm dan 0 ppm (kontrol). Eugenia Volume 18 No. 2  Agustus 2012 Kata kunci : NAA, kakao, mikombi

scholarly journals 664 PB 104 AGROBACTERIUM INFECTION OF WHITE ASH (FRAXINUS AMERICANA L.)

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 528a-528
Author(s):  
Sharon A. Bates ◽  
John E. Preece ◽  
John H. Yopp
Keyword(s):  
Tissue Culture ◽  
Plant Growth ◽  
Growth Regulators ◽  
Callus Formation ◽  
High Humidity ◽  
Ms Medium ◽  
Gall Formation ◽  
White Ash ◽  
Seedling Explants

Both greenhouse-grown white ash plants derived from tissue culture and rooted microshoots in high humidity trays were inoculated with 11 tumor-inducing Agrobacterium strains. Eight strains stimulated mutative gall formation. Plants inoculated with strain A281 exhibited a higher frequency of callus formation (greenhouse-22.2%; microshoots-18.8%) than other strains at the site of the wound. Therefore, strain A281 was used to inoculate seed and seedling explants in vitro. Explants were placed on MS medium containiner no plant growth regulators and inoculated at 0, 3, 5, 7, or 10 days after initiation. Plants inoculated at 10 days showed a higher frequency of callus formation (16.4%) than with earlier inoculations. Also, rewounding of the explant at inoculation resulted in a higher frequency of callus formation (11.3%) compared to not rewounding the explant (3.9%).

scholarly journals Effect of Branch-inducing Treatments on Growth of Tissue Culture and Cutting-Propagated Hydrangea quercifolia ‘Alice’

2014 ◽  
Vol 32 (4) ◽  
pp. 182-188
Author(s):  
Diana R. Cochran ◽  
Marisol Benitez-Ramirez ◽  
Amy Fulcher
Keyword(s):  
Tissue Culture ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Apical Dominance ◽  
Branch Number ◽  
Propagation Technique ◽  
Container Production

Cutting-propagated ‘Alice’ oakleaf hydrangea (Hydrangea quercifolia Bartr.) often produces a few vigorous branches with apical dominance, thus suppressing growth of other branches. As a result, the maturing canopy is sparse and develops asymmetrically, rendering plants unappealing to customers. For this reason, growers prune or apply plant growth regulators (PGRs) to encourage more branching, thereby producing a more desirable product. Propagation through tissue culture may provide another option to increase branching as an outcome of habituation. Habituation occurs when plant cultures continue to respond to a hormone that is no longer being supplied and, in turn, frequently leads to more branching. We evaluated oakleaf hydrangea growth as affected by propagation technique [tissue-cultured (TC) and cutting-propagated (CUT)] and PGR (cyclanilide and benzyladenine) application during container production. Nontreated TC plants had more branches longer than 15.2 cm (6 in) compared to nontreated CUT plants in 2008, although not in 2010. In both years, single applications of cyclanilide did not affect total branch number but two applications increased total branch number compared to nontreated plants, averaged over propagation technique. Plants treated with benzyladenine had similar or fewer total branches compared with nontreated, hand-pruned, and cyclanilide-treated plants (one or two treatments). Propagation technique did not consistently influence response to PGRs.

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