Down-regulation of Pro-inflammatory Mediators by Cladophora wrightiana in Lipopolysaccharide- Stimulated RAW 264.7 Macrophage cells

2014 ◽  
Vol 11 (2) ◽  
pp. 371-378
Author(s):  
Min-Jin Kim ◽  
Mieyang Kim ◽  
Wook Jae Lee ◽  
Sang Suk Kim ◽  
Nam Ho Lee ◽  
...  
Keyword(s):  
Inflammatory Mediators ◽  
Raw 264.7 ◽  
Down Regulation ◽  
Macrophage Cells ◽  
Raw 264.7 Macrophage ◽  
Raw 264.7 Macrophage Cells

Downregulation of pro-inflammatory mediators by a water extract of Schisandra chinensis (Turcz.) Baill fruit in lipopolysaccharide-stimulated RAW 264.7 macrophage cells

2013 ◽  
Vol 36 (2) ◽  
pp. 256-264 ◽  
Author(s):  
Matharage Gayani Dilshara ◽  
Rajapaksha Gedara Prasad Tharanga Jayasooriya ◽  
Chang-Hee Kang ◽  
Seungheon Lee ◽  
Sang Rul Park ◽  
...  
Keyword(s):  
Inflammatory Mediators ◽  
Water Extract ◽  
Raw 264.7 ◽  
Schisandra Chinensis ◽  
Macrophage Cells ◽  
Raw 264.7 Macrophage ◽  
Raw 264.7 Macrophage Cells

scholarly journals Thymol and Piperine-Loaded Poly(D,L-lactic-co-glycolic acid) Nanoparticles Modulate Inflammatory Mediators and Apoptosis in Murine Macrophages

2021 ◽  
Vol 8 (03) ◽  
pp. e122-e130
Author(s):  
Jean Paul Dzoyem ◽  
Nathalie Boulens ◽  
Eric Allémann ◽  
Florence Delie
Keyword(s):  
Inflammatory Mediators ◽  
Glycolic Acid ◽  
In Vitro Assays ◽  
Raw 264.7 ◽  
Griess Reagent ◽  
Macrophage Cells ◽  
Raw 264.7 Macrophage ◽  
Raw 264.7 Macrophage Cells ◽  
Free Molecules

AbstractThis study aimed at preparing and characterizing thymol, eugenol, and piperine-loaded poly(D,L-lactic-co-glycolic acid) nanoparticles and evaluating the effect on inflammatory mediators secretion and apoptosis in Raw 264.7 macrophage cells. Nanoparticles were produced by the solvent evaporation technique. Dynamic light scattering and scanning electron microscopy were used to study the physicochemical characteristics. Raw 264.7 macrophage cells were used as a model for in vitro assays. The 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium assay was used to determine the cytotoxicity of the formulated nanoparticles. An annexin V apoptosis detection kit was used to assess apoptosis. Nitric oxide production was determined using the Griess reagent, and the inflammatory mediators level was evaluated with Th1/Th2 cytokine and fluorometric cyclooxygenase kits. The loaded nanoparticles showed a particle size around 190 nm with a low polydispersity between 0.069 and 0.104 and a zeta potential between–1.2 and–9.5 mV. Reduced cytotoxicity of nanoparticles compared to free molecules against Raw 264.7 macrophage cells was observed and seemed to occur through a mechanism associated with apoptosis. A decrease in cyclooxygenase enzyme activity with an increasing concentration was observed. Both free molecules and nanoparticles showed their capacity to modulate the inflammatory process mostly by inhibiting the investigated inflammatory cytokines. The data presented in this study indicate that thymol and piperine-loaded poly(D,L-lactic-co-glycolic acid nanoparticles could serve as a novel anti-inflammatory colloidal drug delivery system with reduced toxicity. However, further study should be considered to optimize the formulation’s loading capacity and thereby probably enhance their bioactivity in treating inflammatory diseases.

Anti-inflammatory Effects of Madecassic Acid via the Suppression of NF-κB Pathway in LPS-Induced RAW 264.7 Macrophage Cells

Planta Medica ◽  
2009 ◽  
Vol 76 (03) ◽  
pp. 251-257 ◽  
Author(s):  
Jong-Heon Won ◽  
Ji-Sun Shin ◽  
Hee-Juhn Park ◽  
Hyun-Ju Jung ◽  
Duck-Jae Koh ◽  
...  
Keyword(s):  
Raw 264.7 ◽  
Macrophage Cells ◽  
Raw 264.7 Macrophage ◽  
Raw 264.7 Macrophage Cells ◽  
Anti Inflammatory
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