Conjugation of mono-sulfobetaine to alkyne-PPX films via click reaction to reduce cell adhesion

2016 ◽  
Vol 3 (1) ◽  
pp. 59-69
Author(s):  
Hsiu-Wen Chien ◽  
Ming-Chun Keng ◽  
Hsien-Yeh Chen ◽  
Sheng-Tung Huang ◽  
Wei-Bor Tsai
Keyword(s):  
Cell Adhesion ◽  
Click Reaction

Movable Polyrotaxane Surfaces for Modulating Cellular Adhesion via Specific RGD-Integrin Binding

2012 ◽  
Vol 86 ◽  
pp. 59-62
Author(s):  
Ji Hun Seo ◽  
Sachiro Kakinoki ◽  
Tetsuji Yamaoka ◽  
Nobuhiko Yui
Keyword(s):  
Cell Adhesion ◽  
Click Reaction ◽  
Cellular Adhesion ◽  
Important Variable ◽  
Rgd Peptide ◽  
Bioactive Molecules ◽  
Biological Responses ◽  
Cell Adhesive ◽  
Rigid Surfaces ◽  
Chemical Groups

Immobilizing bioactive molecules on the materials surfaces is one of the main strategies for creating functional bio-interfaces. In these kinds of bio-interfaces, the density of immobilized functional groups and the following physicochemical factors such as roughness, polarity and electrical charge have been thought important variables for regulating biological responses such as cell adhesion and differentiations. Here in this study, differences between rigidity and dynamically immobilized bioactive molecules on the biological responses will be discussed. In order to develop dynamic bio-interfaces, a polyrotaxane based block-copolymer containing clickable azide groups for conjugating various bioactive molecules was designed. Cell adhesive RGD peptide was then conjugated with the azide group by click reaction on both dynamic and rigid surfaces. As a result, cell adhesive RGD peptide immobilized on the dynamic bio-interfaces shows larger initial cell adhesion area, indicating that molecular dynamics of surface chemical groups is another important variable for the regulation of biological responses.

scholarly journals Polycaprolactone strengthening keratin/bioactive glass composite scaffolds with double cross-linking networks for potential application in bone repair

2022 ◽  
Vol 4 (1) ◽  
Author(s):  
Liying Sun ◽  
Shan Li ◽  
Kaifeng Yang ◽  
Junchao Wang ◽  
Zhengjun Li ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Bioactive Glass ◽  
Bone Repair ◽  
Composite Scaffold ◽  
Click Reaction ◽  
Epoxy Group ◽  
Cross Linking ◽  
Glass Composite ◽  
Composite Scaffolds ◽  
Double Cross

AbstractIn this study, we aimed at constructing polycaprolactone (PCL) reinforced keratin/bioactive glass composite scaffolds with a double cross-linking network structure for potential bone repair application. Thus, the PCL-keratin-BG composite scaffold was prepared by using keratin extracted from wool as main organic component and bioactive glass (BG) as main inorganic component, through both cross-linking systems, such as the thiol-ene click reaction between abundant sulfhydryl groups of keratin and the unsaturated double bond of 3-methacryloxy propyltrimethoxy silane (MPTS), and the amino-epoxy reaction between amino groups of keratin and the epoxy group in (3-glycidoxymethyl) methyldiethoxysilane (GPTMS) molecule, along with introduction of PCL as a reinforcing agent. The success of the thiol-ene reaction was verified by the FTIR and 1H-NMR analyses. And the structure of keratin-BG and PCL-keratin-BG composite scaffolds were studied and compared by the FTIR and XRD characterization, which indicated the successful preparation of the PCL-keratin-BG composite scaffold. In addition, the SEM observation, and contact angle and water absorption rate measurements demonstrated that the PCL-keratin-BG composite scaffold has interconnected porous structure, appropriate pore size and good hydrophilicity, which is helpful to cell adhesion, differentiation and proliferation. Importantly, compression experiments showed that, when compared with the keratin-BG composite scaffold, the PCL-keratin-BG composite scaffold increased greatly from 0.91 ± 0.06 MPa and 7.25 ± 1.7 MPa to 1.58 ± 0.21 MPa and 14.14 ± 1.95 MPa, respectively, which suggesting the strong reinforcement of polycaprolactone. In addition, the biomineralization experiment and MTT assay indicated that the PCL-keratin-BG scaffold has good mineralization ability and no-cytotoxicity, which can promote cell adhesion, proliferation and growth. Therefore, the results suggested that the PCL-keratin-BG composite scaffold has the potential as a candidate for application in bone regeneration field. Graphical Abstract

622: Von Hippel-Lindau Inactivation Downregulates the Cell-Cell Adhesion Molecule E Cadherin in Renal Cancer Cells

2005 ◽  
Vol 173 (4S) ◽  
pp. 170-170
Author(s):  
Maxine G. Tran ◽  
Miguel A. Esteban ◽  
Peter D. Hill ◽  
Ashish Chandra ◽  
Tim S. O'Brien ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Cancer Cells ◽  
Renal Cancer ◽  
Adhesion Molecule ◽  
Cell Adhesion Molecule ◽  
Von Hippel Lindau ◽  
E Cadherin ◽  
Cell Cell
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