Cell disruption and lipid extraction from microalgae Amphiprora sp. using acid hydrolysis-solvent extraction route

2017 ◽  
Vol 10 ◽  
pp. 841-849 ◽  
Author(s):  
Angel Dario Gonzalez-Delgado ◽  
Janet Bibiana Garcia Martinez ◽  
Yeimmy Yolima Peralta-Ruiz
Keyword(s):  
Solvent Extraction ◽  
Acid Hydrolysis ◽  
Lipid Extraction ◽  
Cell Disruption

scholarly journals DEVELOPMENT AND EVALUATION OF THERMAL AND CHEMICAL MICROALGAE CELL DISRUPTION METHODS FOR BIOFUELS AND BIOPRODUCTS PRODUCTION

2015 ◽  
Vol 27 (2) ◽  
pp. 81-90
Author(s):  
Angel González Delgado ◽  
Yeimmy Yolima Peralta Ruiz ◽  
Viatcheslav Kafarov
Keyword(s):  
Acid Hydrolysis ◽  
Extraction Efficiency ◽  
Lipid Extraction ◽  
Cell Disruption ◽  
Organosolv Pretreatment ◽  
Promising Candidate ◽  
Thermal Cell ◽  
Extraction Processes ◽  
Pretreated Biomass ◽  
Metabolite Extraction

Biomass of microalgae continues under study as promising candidate for novel biofuels and bioproducts, however, microalgae-based processes faces technical bottlenecks related to efficiency of metabolite  extraction and transformation. This paper shows the development and evaluation of thermal and chemical cell disruption methods for the extraction of oil from  Nannochloropsis  sp.  as  feedstock  for the  production of third generation biofuels and high value fatty acids, in order to achieve lipid extraction efficiency. The thermal cell disruption process chosen was autoclave, during operation times of 1 and 3 hours at 394.15 K and 103410 Pa. Chemical treatment was  based on acid  hydrolysis with  HCl in concentrations  of  0.1,  0.5, 1  and 3 mol L-1. Organosolv Pretreatment was also used with a reaction time of 4.1 hours at 394.15 K and  103410  Pa. Results showed that the thermal and Organosolv cell disruption process increases extraction yields for biomass without pretreatment, but these did not exceed any of the results obtained with acid hydrolysis. Solution 0.5 mol L-1 HCl proved to be the most suitable for cell disruption process.  In  addition it was determined that the water content in the pretreated biomass, decreases the efficiency of the extraction processes.

Microalgal Cell Disruption and Lipid Extraction Techniques for Potential Biofuel Production

2020 ◽  
pp. 129-147
Author(s):  
Ahasanul Karim ◽  
M. Amirul Islam ◽  
Zaied Bin Khalid ◽  
Che Ku Mohammad Faizal ◽  
Md. Maksudur Rahman Khan ◽  
...  
Keyword(s):  
Lipid Extraction ◽  
Cell Disruption ◽  
Biofuel Production ◽  
Extraction Techniques

An improved extraction method of 3-oh fatty acids for environmental reconstruction

2020 ◽  
Author(s):  
Chanamon Panbut
Keyword(s):  
Fatty Acids ◽  
Acid Hydrolysis ◽  
Lipid A ◽  
Environmental Changes ◽  
Solid Phase ◽  
Lipid Extraction ◽  
Heating Time ◽  
Hydroxy Fatty Acids ◽  
Environmental Reconstruction ◽  
Total Lipid Extraction

<p>3-hydroxy or beta-hydroxy fatty acids produced by Gram-negative bacteria are a novel proxy for assessment of the environmental changes. These compounds composed of lipopolysaccharide (LPS) of Lipid A, a core polysaccharide region, and an O-antigen polysaccharide chain. The improved method for the 3-hydroxy fatty acids extraction was proposed in this study. The 12 soil samples collected from the eastern US border along the coastline from Maine to Florida were generally processed by acid hydrolysis, methylation, total lipid extraction, and solid-phase chromatography, respectively. Fatty acids eventually can be separated from the main part of LPS and combined with a methyl group. However, in the stage of acid hydrolysis, the temperature was decreased to 55 °C, and heating time was extended in order to prevent the broken of volatile compounds and diminish the relative abundance of 3-OH fatty acids. The higher abundance of interested 3-OH fatty acids for the environmental reconstruction can potentially be extracted by this improvement than the classical protocol. This research will be further compared in terms of cost, experimental time and completeness of data between these two methods.</p>

The effects of microalgal cell disruption via FeCl3-based synergistic effect between Fenton-like and Lewis acid reaction for lipid extraction

2014 ◽  
Vol 6 ◽  
pp. 86-90 ◽  
Author(s):  
Dong-Yeon Kim ◽  
Ji-Yeon Park ◽  
Sun-A Choi ◽  
You-Kwan Oh ◽  
Il-Gyu Lee ◽  
...  
Keyword(s):  
Synergistic Effect ◽  
Lewis Acid ◽  
Lipid Extraction ◽  
Cell Disruption ◽  
Acid Reaction

Cell disruption and lipid extraction for microalgal biorefineries: A review

2017 ◽  
Vol 244 ◽  
pp. 1317-1328 ◽  
Author(s):  
Soo Youn Lee ◽  
Jun Muk Cho ◽  
Yong Keun Chang ◽  
You-Kwan Oh
Keyword(s):  
Lipid Extraction ◽  
Cell Disruption

Microalgae harvesting and cell disruption: a preliminary evaluation of the technology electroflotation by alternating current

2014 ◽  
Vol 70 (2) ◽  
pp. 315-320 ◽  
Author(s):  
Riamburgo Gomes de Carvalho Neto ◽  
José Gilmar da Silva do Nascimento ◽  
Mayara Carantino Costa ◽  
Alexandre Colzi Lopes ◽  
Eliezer Fares Abdala Neto ◽  
...  
Keyword(s):  
Lipid Extraction ◽  
Alternating Current ◽  
Cell Disruption ◽  
Biodiesel Production ◽  
Attractive Potential ◽  
Preliminary Evaluation ◽  
Waste Stabilization Ponds ◽  
High Productivity ◽  
Microalgae Harvesting ◽  
Batch Time

Some species of microalgae have high productivity and lipid content, which makes them good candidates for biodiesel production. Biomass separation and cell disruption are important steps in biodiesel production from microalgae. In this work, we explored the fundamentals of electroflotation by alternating current (EFAC) with non-consumable electrodes to simultaneously harvest microalgae and disrupt cells from mixed microalgae obtained from waste stabilization ponds. The harvesting efficiency was evaluated using chlorophyll-a and turbidity, which reached removals of 99% and 95%, respectively, during a batch time of 140 min. Cell disruption was evaluated using lipid extraction, and the best results were achieved with a batch time of 140 min, which resulted in a 14% yield. Therefore, EFAC was shown to be an attractive potential technology for simultaneous microalgal harvesting and cell disruption.

Novel strategy for microalgae cell disruption and wet lipid extraction by employing electro-Fenton process with sacrificial steel anode

2021 ◽  
pp. 126110
Author(s):  
Wanniarachchige Paramitha Sandani ◽  
Malith Premaratne ◽  
Thilini U. Ariyadasa ◽  
Jagath Kumara Premachandra
Keyword(s):  
Lipid Extraction ◽  
Cell Disruption ◽  
Fenton Process ◽  
Wet Lipid Extraction ◽  
Novel Strategy
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