scholarly journals New Stability Indicating Method for the Simultaneous Determination of Impurities Present in Candesartan Cilexetil and Hydrochlorothiazide Tablets by Ultra Performance Liquid Chromatography with Photo Diode Array Detector

2016 ◽  
Vol 12 (2) ◽  
pp. 127-149
Author(s):  
M. V. V. N. Murali Krishna ◽  
Sumathi Rao ◽  
N. V. S. Venugopal ◽  
Bhaskara Mantena
Keyword(s):  
Liquid Chromatography ◽  
Simultaneous Determination ◽  
Candesartan Cilexetil ◽  
Ultra Performance Liquid Chromatography ◽  
Array Detector ◽  
Diode Array ◽  
Diode Array Detector ◽  
Stability Indicating Method ◽  
Determination Of Impurities

scholarly journals FAST AND SIMPLE METHOD FOR SIMULTANEOUS DETERMINATION OF BENZOIC ACID AND ACESULFAME-K IN SOFT DRINKS BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY-DIODE ARRAY DETECTOR: METHOD VALIDATION AND UNCERTAINTY EVALUATION

2018 ◽  
Vol 15 (29) ◽  
pp. 96-105
Author(s):  
D. PUTRI ◽  
Y. ARISTIAWAN ◽  
N. ARYANA ◽  
D. STYARINI
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Benzoic Acid ◽  
Simultaneous Determination ◽  
High Performance ◽  
Array Detector ◽  
Diode Array ◽  
Fast Method ◽  
Diode Array Detector

Classified as food additives, the use of benzoic acid and acesulfame K to preserve foods is finite due to health and environmentreason.In this study, anefficient and fast method for simultaneous determination of benzoic acid and acesulfame-K in soft drinks was validated using high performance liquid chromatography with diode array detector (HPLC-DAD) on a C18 column. The method showed results satisfaction as the coefficient determination for benzoic acid and acesulfame-K were 0.9998 and 0.9991 respectively. The LOD for both compounds found at 0.02 mg/kg and the LOQ were found at 0.07 and 0.06 mg/kg for benzoic acid and acesulfame-K respectively. The recovery studies proved that the method has a good recovery ranging from 96103% for both benzoic acid and acesulfame-K with %RSD varies from 1.6 - 4.6% at different concentrations. The validated method then applied to the inter-laboratory comparison sample conducted by the National Quality Control Laboratory for Drug and Food (NQCLDF), Indonesia, using traceable standard reference materials. Uncertainty evaluation using bottom-up approach showed that the method has reasonable uncertainty value with uncertainty budget derived from weighing process, recovery studies, method precision, calibration curve and standard solution. The uncertainty value (k=2, in confidence level 95%) for benzoic acid was found to be 4% at reported concentration of 343 mg/kg and for acesulfame-K was found to be 6.5% at reported concentration of 195 mg/kg. In conclusion, the validated method offered a simple and fast method, as well as economical reagents consumption indicating its possible application as an alternative method for a routine analysis.

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