Characterization of the Potent Mannitol Producer Leuconostoc mesenteroides KCTC13305

2018 ◽  
Vol 30 (1) ◽  
Keyword(s):  
Leuconostoc Mesenteroides

Identification and functional characterization of levS, a gene encoding for a levansucrase from Leuconostoc mesenteroides NRRL B-512 F

Gene ◽  
2006 ◽  
Vol 376 (1) ◽  
pp. 59-67 ◽  
Author(s):  
Sandra Morales-Arrieta ◽  
Maria Elena Rodríguez ◽  
Lorenzo Segovia ◽  
Agustín López-Munguía ◽  
Clarita Olvera-Carranza
Keyword(s):  
Leuconostoc Mesenteroides ◽  
Functional Characterization ◽  
Gene Encoding

scholarly journals Characterization of the Different Dextransucrase Activities Excreted in Glucose, Fructose, or Sucrose Medium byLeuconostoc mesenteroides NRRL B-1299

1998 ◽  
Vol 64 (4) ◽  
pp. 1298-1302 ◽  
Author(s):  
Marguerite Dols ◽  
M. Remaud-Simeon ◽  
R. M. Willemot ◽  
M. Vignon ◽  
P. Monsan
Keyword(s):  
Gel Electrophoresis ◽  
Leuconostoc Mesenteroides ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Traditional Culture ◽  
Culture Conditions ◽  
Acceptor Reaction ◽  
Sucrose Medium ◽  
Molecular Masses

ABSTRACT When grown in glucose or fructose medium in the absence of sucrose,Leuconostoc mesenteroides NRRL B-1299 produces two distinct extracellular dextransucrases named glucose glucosyltransferase (GGT) and fructose glucosyltransferase (FGT). The production level of GGT and FGT is 10 to 20 times lower than that of the extracellular dextransucrase sucrose glucosyltransferase (SGT) produced on sucrose medium (traditional culture conditions). GGT and FGT were concentrated by ultrafiltration before sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis. Their molecular masses were 183 and 186 kDa, respectively, differing from the 195 kDa of SGT. The structural analysis of the dextran produced from sucrose and of the oligosaccharides synthesized by acceptor reaction in the presence of maltose showed that GGT and FGT are two different enzymes not previously described for this strain. The polymer synthesized by GGT contains 30% α(1→2) linkages, while FGT catalyzes the synthesis of a linear dextran only composed of α(1→6) linkages.

scholarly journals Development and Physical Characterization of α-Glucan Nanoparticles

Molecules ◽  
2020 ◽  
Vol 25 (17) ◽  
pp. 3807
Author(s):  
Kervin O. Evans ◽  
Christopher Skory ◽  
David L. Compton ◽  
Ryan Cormier ◽  
Gregory L. Côté ◽  
...  
Keyword(s):  
High Pressure ◽  
Glass Transition ◽  
Transition Temperature ◽  
Leuconostoc Mesenteroides ◽  
Elevated Temperatures ◽  
High Pressure Homogenization ◽  
Hydrophobic Core ◽  
Fluorescence Measurements ◽  
Exposure Measurements

α-Glucans that were enzymatically synthesized from sucrose using glucansucrase cloned from Leuconostoc mesenteroides NRRL B-1118 were found to have a glass transition temperature of approximately 80 °C. Using high-pressure homogenization (~70 MPa), the α-glucans were converted into nanoparticles of ~120 nm in diameter with a surface potential of ~−3 mV. Fluorescence measurements using 1,6-diphenyl-1,3,5-hexatriene (DPH) indicate that the α-glucan nanoparticles have a hydrophobic core that remains intact from 10 to 85 °C. α-Glucan nanoparticles were found to be stable for over 220 days and able to form at three pH levels. Accelerated exposure measurements demonstrated that the α-glucan nanoparticles can endure exposure to elevated temperatures up to 60 °C for 6 h intervals.

Structural analysis and characterization of dextran produced by wild and mutant strains of Leuconostoc mesenteroides

2014 ◽  
Vol 99 ◽  
pp. 331-338 ◽  
Author(s):  
Nadir Naveed Siddiqui ◽  
Afsheen Aman ◽  
Alba Silipo ◽  
Shah Ali Ul Qader ◽  
Antonio Molinaro
Keyword(s):  
Structural Analysis ◽  
Leuconostoc Mesenteroides ◽  
Mutant Strains
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