scholarly journals Frequent Detection of Y Chromosome Sequences in Japanese Turner's Syndrome by Southern Blot Analysis of Amplified DNA

1996 ◽  
Vol 5 (2) ◽  
pp. 83-87
Author(s):  
Yuichi Nakagawa ◽  
Hiromune Natsume ◽  
Akira Kubota ◽  
Hiromi Takeuchi ◽  
Kaoru Nasuda ◽  
...  
Keyword(s):  
Southern Blot ◽  
Y Chromosome ◽  
Blot Analysis ◽  
Southern Blot Analysis ◽  
Turner's Syndrome ◽  
Turner’S Syndrome

Detection of Y chromosome sequences in Turner's syndrome by Southern blot analysis of amplified DNA

The Lancet ◽  
1993 ◽  
Vol 342 (8864) ◽  
pp. 140-143 ◽  
Author(s):  
M. Kocova ◽  
S.F. Siegel ◽  
M. Trucco ◽  
S.F. Siegel ◽  
P.A. Lee ◽  
...  
Keyword(s):  
Southern Blot ◽  
Y Chromosome ◽  
Blot Analysis ◽  
Southern Blot Analysis ◽  
Turner's Syndrome ◽  
Turner’S Syndrome

scholarly journals Normal structure and expression of Zfy genes in XY female mice mutant in Tdy

Development ◽  
1990 ◽  
Vol 109 (3) ◽  
pp. 647-653 ◽  
Author(s):  
J. Gubbay ◽  
P. Koopman ◽  
J. Collignon ◽  
P. Burgoyne ◽  
R. Lovell-Badge
Keyword(s):  
Southern Blot ◽  
Y Chromosome ◽  
Blot Analysis ◽  
Southern Blot Analysis ◽  
Normal Structure ◽  
Structural Alterations ◽  
Female Mice ◽  
Testis Determination ◽  
Zfy Genes ◽  
Xy Female

Zfy-1 and Zfy-2 are candidate genes for Tdy, the testis-determining gene in mice. We have analysed these genes in a line of XY female mice that have been shown to be mutated in Tdy. We have used Southern blot analysis to show that the Zfy genes have not undergone any major structural alterations, and have also demonstrated that both genes are transcribed normally from the mutant Y chromosome (Y) in both adult XYY testis and XY female embryonic gonads. The fact that these genes show a normal structure and expression pattern in mice with a Y chromosome known to carry a mutation in Tdy and that mutant embryos develop into females despite Zfy-1 expression, strongly supports other recent evidence that Zfy genes are not directly involved in primary testis determination.

scholarly journals Long-term engraftment of normal and post-5-fluorouracil murine marrow into normal nonmyeloablated mice [see comments]

Blood ◽  
1993 ◽  
Vol 81 (10) ◽  
pp. 2566-2571 ◽  
Author(s):  
FM Stewart ◽  
RB Crittenden ◽  
PA Lowry ◽  
S Pearson-White ◽  
PJ Quesenberry
Keyword(s):  
Southern Blot ◽  
Blot Analysis ◽  
Southern Blot Analysis ◽  
Normal Male ◽  
Normal Donor ◽  
Long Term Stability ◽  
Donor Bone Marrow ◽  
Male Donor ◽  
Marrow Cells

We report the successful long-term engraftment of normal male donor bone marrow (BM) transfused into noncytoablated female mice, challenging the assumption that “niches” need to be created for marrow to engraft. We have used chromosomal banding and Southern blot analysis to identify transplanted male marrow cells, and shown the long-term stability of the chimeric marrows. Balb/C, BDF1, or CBA-J female hosts (no irradiation) received for 5 consecutive days 40 x 10(6) male cells (per day) of the same strain, and repopulation patterns were observed. Parallel studies were performed using tibia/femur equivalents of normal marrow or marrow from Balb/C mice pretreated 6 days previously with 150 mg/kg 5-fluorouracil (5-FU). Chromosome banding techniques showed that 5% to 46% of marrow cells were male 3 to 9 months posttransplant with normal donor marrow. Southern blot analysis, using the pY2 probe, showed continued engraftment at 21 to 25 months posttransplant, ranging from 15% to 42% male engrafted cells in marrow. Normal donor male marrow engrafted significantly better than 5-FU-pretreated male marrow as shown 1 to 12 months posttransplant in non-cytoablated female recipients. Percentages of male engrafted cells in BM ranged from 23% to 78% for recipients of normal donor marrow and from 0.1% to 39% for recipients of 5-FU marrow. Mean engraftment for 6 mice receiving normal marrow was 38%, whereas that for 6 mice receiving post-5-FU marrow was 8%, as assayed 1 to 3 months posttransplant. At 10 to 12 months, mean engraftment for the normal donor group was 46%, compared with 16% for the 5-FU group. The patterns of engraftment with normal and 5-FU marrow were similar for spleen and thymus. These results show that long-term chimerism can be established after transplantation of normal donor marrow to normal nonirradiated host mice and indicate that marrow spaces do not have to be created for successful engraftment. They suggest that transplanted marrow competes equally with host marrow for marrow space. Finally, these data show that post-5-FU Balb/C male marrow is markedly inferior in the repopulation of Balb/C female host marrow, spleen, and thymus, and suggest that this population of cells may not be the ideal population for gene transfer studies.

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