scholarly journals ESTIMATION OF THE TIME SINCE DEATH BY USING THE POLARIZATION MULLER-MATRIX MICROSCOPY OF BIOLOGICAL TISSUE IN CASES OF ALCOHOL AND CARBON MONOXIDE POISONING

2020 ◽  
Vol 1 ◽  
pp. 41-47
Author(s):  
Ihor Ivaskevich ◽  
Oleh Vanchuliak ◽  
Viktor Bachynskіy ◽  
Sviatoslava Yurniuk
Keyword(s):  
Carbon Monoxide ◽  
Adrenal Glands ◽  
Carbon Monoxide Poisoning ◽  
Biological Tissues ◽  
Time Since Death ◽  
Diagnostic Efficiency ◽  
Histological Sections ◽  
Sensitivity Range ◽  
Diagnostic Capabilities ◽  
The Brain

Introduction: This paper aims to investigate and substantiate the information capabilities of the objective method of azimuthal-invariant polarization Müller-matrix microscopy of histological sections of biological tissues (BT) in the diagnosis of time since death (TSD) in alcohol and monoxide poisoning. Objectives: The purpose of the study was to evaluate the diagnostic capabilities of the azimuthal-invariant Muller-matrix polarimetry technique of the polycrystalline constituent of BT and human body fluids in determining the TSD in cases of alcohol and carbon monoxide poisoning. Results: A comprehensive experimental study of the diagnostic efficiency of the azimuthal-invariant Muller-matrix polarimetric microscopy of the polycrystalline component of histological sections of the brain, myocardium, adrenal glands, liver, and polycrystalline blood films from dead persons for the diagnosis of TSD in cases of alcohol and carbon monoxide poisoning.A sensitivity range of 24 hours was determined using the method of the azimuthal-invariant Muller-matrix polarimetric microscopy of the polycrystalline component of histological sections of the myocardium for the diagnosis of TSD in cases of alcohol and carbon monoxide poisoning. Conclusion: The results showed the effectiveness of the studied methodology and the prospect of further research in this direction.

scholarly journals MAPPING OF AZIMUTHS OF POLARIZATION OF LASER MICROSCOPIC IMAGES OF HISTOLOGICAL SECTIONS OF HUMAN RGANS IN THE DIFFERENTIATION OF POISONING BY ETHANOL AND CARBON MONOXIDE

2019 ◽  
Vol 43 (4) ◽  
pp. 70-74
Author(s):  
I.B. Ivaskevych ◽  
V. T. Bachynskyy ◽  
O. Y. Vanchulyak ◽  
O. H. Palyvoda
Keyword(s):  
Carbon Monoxide ◽  
Adrenal Glands ◽  
Statistical Processing ◽  
Biological Tissues ◽  
Statistical Moments ◽  
Co Poisoning ◽  
Microscopic Images ◽  
Histological Sections ◽  
The Brain ◽  
Keywords Laser

Abstract Purpose of the study. This work is aimed at research and substantiation of information possibilities of objective method of polarization mapping of microscopic images of histological sections of human biological tissues for the establishment and differentiation of С2Н5ОН and CO poisoning. Materisls and methods. Native histological sections of the brain, myocardium and adrenal glands from ethanol poisoning (n = 30) and CO (n = 20) were investigated using a laser polarimeter of standard scheme. Subsequently, statistical processing of the obtained results was conducted. Results. Statistical moments of 1–4th order were determined by the method of polarization mapping of microscopic images of histological sections of human biological tissues. The values of the statistical moments of the 1–4th order for brain tissue for С2Н5ОН and CO poisoning reach differences by 2–2,5 times, for myocardial tissue – 2,2–2,9 times, the adrenal glands – 1,4–1,8 times. Accordingly, the statistical moments of the 3rd and 4th order are more sensitive to the detection and differentiation of poisons, whereby the accuracy of the method of differential diagnosis of poisoning С2Н5ОН and CO reaches an adequate level (~ 90%) in the study of histological sections of the myocardium and brain. Conclusion. The obtained results show that the method of polarization mapping of microscopic images of human biological tissues gives an objective possibility of reliable establishment and differentiation of С2Н5ОН and СО poisoning. Keywords: laser polarimetry, poisoning, ethanol, carbon monoxide, forensic medicine.

scholarly journals FORENSIC MEDICAL CRITERIA OF DIFFERENTIAL DIAGNOSIS OF ALCOHOL AND CARBON MONOXIDE POISONING BY USING AZIMUTHAL-INVARIANT POLARIZATION MUELLER-MATRIX MICROSCOPY

2021 ◽  
Vol 2 ◽  
pp. 33-40
Author(s):  
Olexandr Harazdiuk ◽  
Ihor Ivaskevich ◽  
Oleh Vanchuliak ◽  
Yuliia Sarkisova
Keyword(s):  
Carbon Monoxide ◽  
Differential Diagnosis ◽  
Cause Of Death ◽  
Human Body ◽  
Carbon Monoxide Poisoning ◽  
Biological Tissues ◽  
Mueller Matrix ◽  
Histological Sections ◽  
Common Causes ◽  
Characteristic Features

Introduction: Fatal intoxications with alcohol and carbon monoxide are the most common causes of poisoning that occur in forensic medical practice. Therefore, the differential diagnosis of these intoxications is extremely important. To solve this problem, we propose the use of the method of azimuthal-invariant polarization Mueller-matrix microscopy of histological sections of biological tissues (BT). Objectives: The purpose of the study was to establish statistically significant criteria for differentiation of the cause of death in case of alcohol poisoning (AP) and carbon monoxide poisoning (CMP) by using azimuthal-invariant polarization Mueller-matrix microscopy of polycrystalline fractions of histological sections of organs and blood films of the human body. Results: A number of characteristic features have been identified that have not been previously described and allow to objectively differentiate the cause of death in the case of AP and CMP, namely Ek – 0.95±0.045 and 1.81±0.092 in the study of histological sections of the myocardium;  Ek – 0,19±0,011 and 1,11±0,094 – polycrystalline blood films. Analysis of the obtained data revealed a good level of accuracy in the study of histological sections of the myocardium (As 85%) and polycrystalline blood films (As 88%). Conclusion: Thus, developed and tested method of azimuthal-invariant polarization Mueller-matrix microscopy allows to differentiate the cause of death in cases of AP and CMP.

Application of Perfluorooctylbromide Nanoparticles with Ulinastatin for Early Brain Injury Caused by Carbon Monoxide Poisoning

2021 ◽  
Vol 13 (7) ◽  
pp. 1383-1390
Author(s):  
Guangcong Li ◽  
Dan Li
Keyword(s):  
Carbon Monoxide ◽  
Particle Size ◽  
Treatment Group ◽  
Cell Apoptosis ◽  
High Speed ◽  
Carbon Monoxide Poisoning ◽  
Neuronal Cell ◽  
Early Brain Injury ◽  
Group A ◽  
The Brain

ABSTRACTThis study aimed to explore the mechanism of perfluorooctylbromide (PFOB) nanoparticles (NPs) combined with ulinastatin (UTI) on early brain injury (EBI) caused by carbon monoxide poisoning (CMP). Firstly, PFOB NPs were prepared by high-speed dispersion and high-speed homogenization. The physicochemical characteristics of the particle size distribution and Zeta potential distribution of the NPs were analyzed using a laser particle size analyzer. The thermal and photoinduced phase transition characteristics of the NPs were analyzed under heating and laser irradiation conditions. Then, 50 Sprague Dawley (SD) rats were deemed as the research objects to establish the CMP rat models using hyperbaric oxygen chambers. According to different treatment methods, they were rolled into a healthy control group, a carbon monoxide (CO) model group, a PTOB treatment group, an UTI treatment group, and a PTOB + UTI treatment group. The brain tissues of each group of rats were collected 3 days after treatment. The neuronal cell apoptosis, expression of Caspase-3, messenger ribonucleic acid (mRNA) of inflammatory factors interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) in rat brain tissue were detected through immunohistochemical staining, in situ cell apoptosis detection, Reverse transcription-polymerase chain reaction (RT-PCR), and Western blotting, so did the relative expression of target proteins B-cell lymphoma-2 (Bcl-2), Bcl2-Associated X (Bax) and myelin basic protein (MBP). As a result, the average particle size and the average Zeta potential of the prepared PFOB NPs was 103±31 nm and −23 ± 15 mV, respectively. When the PFOB NPs were heated to 80 °C, the particle size increased greatly and cracks appeared. The particle size of PFOB NPs also increased obviously after laser irradiation, and the PFOB inside the particles changed into gas phase. Compared to CO group, expression of Caspase-3, neuronal cell apoptosis rate, mRNA expression of IL-1β and TNF-α, and protein expression of Bax and Bcl-2 in the brain tissue of PTOB group, UTI group, and PFOB + UTI group were notably decreased (P < 0.05), while the MBP protein expression increased considerably (P < 0.05). Changes in PFOB + UTI group were more obvious than those in PTOB group and UTI group, and those indicators weren’t considerably different from the controls. In summary, PFOB NPs were successfully prepared with favorable phase transition characteristics. Moreover, PFOB NPs combined with UTI could reduce the apoptosis of brain neurons after CMP, improve the inflammatory response, and play a protective effect on EBI of CMP.

Brain stem and spinal cord demyelination due to acute carbon monoxide poisoning

2021 ◽  
pp. 247-253
Author(s):  
Yan Lv ◽  
Yv Zhang ◽  
Shuyi Pam ◽  
Keyword(s):  
Spinal Cord ◽  
Carbon Monoxide ◽  
Brain Stem ◽  
Carbon Monoxide Poisoning ◽  
Recovery Period ◽  
Extended Period ◽  
Severe Case ◽  
Co Poisoning ◽  
Secondary Demyelination ◽  
The Brain

Demyelination throughout the brain stem and spinal cord caused by acute carbon monoxide (CO) poisoning has not been previously reported. Magnetic resonance imaging (MRI) has revealed that acute CO poisoning primarily affects the subcortical white matter of the bilateral cerebral hemispheres and basal ganglia. Here we report the case of a patient with delayed neuropsychological sequelae (DNS) due to acute CO poisoning. A 28-year-old man was admitted to our department following a suicide attempt by acute CO poisoning. After a six-month pseudo-recovery period, he was diagnosed with DNS, with MRI evidence of demyelinating change of the bilateral cerebral peduncles. Demyelination was identified throughout the brain stem, expanding from the bilateral cerebral peduncles to the medulla oblongata, occurring approximately six months after poisoning. One and a half years after acute CO poisoning, demyelination of the cervical and thoracic spine was observed, most notable in the lateral and posterior cords. It is evident that previously published research on this topic is extremely limited. Perhaps in severe cases of acute CO poisoning the fatality rate is higher, leading to fewer surviving cases for possible study. This may be because a more severe case of acute CO poisoning would result in the higher likelihood of secondary demyelination. This research indicates that clinicians should be aware of the risk of secondary demyelination and take increased precautions such as vitamin B supplementation and administration of low-dose corticosteroids for an extended period of time in order to reduce the extent and severity of demyelination.

Computed tomography of the brain in acute carbon monoxide poisoning

1996 ◽  
Vol 51 (7) ◽  
pp. 480-483 ◽  
Author(s):  
D.A.T. Silver ◽  
M. Cross ◽  
B. Fox ◽  
R.M. Paxton
Keyword(s):  
Computed Tomography ◽  
Carbon Monoxide ◽  
Carbon Monoxide Poisoning ◽  
Acute Carbon Monoxide Poisoning ◽  
The Brain

scholarly journals Differential diagnostics of the presence of damage by the method of digital polarization mapping of microsocopy images of hystological sections of human organs

2021 ◽  
pp. 70-78
Author(s):  
Oleksandra Litvinenko ◽  
Oleh Vanchulyak ◽  
Irina Soltys ◽  
Olga Mikhailova ◽  
Artem Motrich
Keyword(s):  
Lung Tissue ◽  
Experimental Testing ◽  
Biological Tissues ◽  
Differential Diagnostics ◽  
Time Interval ◽  
Diagnostic Efficiency ◽  
Internal Organs ◽  
Microscopic Images ◽  
Histological Sections ◽  
Ellipticity Of Polarization

The article presents the results of experimental testing of methods of azimuthal-invariant polarization mapping of microscopic images of samples of histological sections of the myocardium and lung tissue; time monitoring of changes in the magnitude of statistical moments of the 1-4th orders, characterizing the distributions of the azimuth and ellipticity of polarization of microscopic images of myocardial and lung tissue samples with different age of damage; determination of the diagnostic efficiency (time interval and accuracy) of establishing the age of damage to human internal organs by digital histological methods of mapping azimuth maps and polarization ellipticity of microscopic images. Aim of the work. Development of a digital histology technique for samples of human internal organs. Materials and methods. The object of the study was the histology of samples of human internal organs (myocardium, lung tissue) with different duration of damage from 1 hour to 120 hours. For control, we used BT samples from those who died from coronary artery disease with different duration of damage from 1 hour to 120 hours. The studies were carried out using the method of digital polarization mapping of microscopic images of histological sections of biological tissues of human internal organs. Results. Revealed the following ranges ramp variation quantity statistics polarizing digital histology and accuracy of the limitation of damage: a. Arts azimuth polarization microscopic image of a magnification ×40 (asymmetry – 12 hours, kurtosis – 12 hours, accuracy – 55-60 min), maps of ellipticity of polarization of microscopic images with a magnification of ×40 (asymmetry – 12 hours, kurtosis – 12 hours, accuracy – 65-75 min). Conclusions. Scenarios of changes in the statistical structure of maps of azimuth and ellipticity of polarization of microscopic images of histological sections of human internal organs have been determined - with an increase in the duration of damage, the value of the mean and variance decreases, the asymmetry and kurtosis increase.

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