scholarly journals VECTOR-BORNE PATHOGENS-TESTING IN A ROMANIAN CANINE BLOOD BANK

2018 ◽  
Vol 6 ◽  
pp. 904-911
Author(s):  
Teodor-Stefan Ionescu ◽  
Sinziana Radulescu ◽  
Ruxandra Florea ◽  
Stelian Baraitareanu ◽  
Doina Danes
Keyword(s):  
Blood Donors ◽  
Holistic Approach ◽  
Blood Bank ◽  
Focused Attention ◽  
Donor Pool ◽  
Blood Samples ◽  
Negative Results ◽  
Blood Banks ◽  
Vector Borne ◽  
Positive Results

INTRODUCTION: Canine blood banking in veterinary medicine is an expanding market. Once the demand for blood products increased all over the world, canine blood banks have focused attention on the risk of spreading diseases through blood transfused products. The need to preserve a healthy donor-pool, free of blood-borne infectious diseases, mainly in endemic areas, led to the implementation of appropriate protocols for screening canine blood donors using specific tests.OBJECTIVES: The aim of this study was to evaluate the presence of Anaplasma phagocytophilum/Anaplasmaplatys, Echrlichiacanis/Echrlichiaewingii, Dirofilariaimmitis and Borrelia burgdorferi using the enzyme immunoassay technology (EIA) among the donors of a Romanian canine blood bank, from January 2015 to December 2016.METHODS: Blood samples from 575 donors were collected and 1253 tests were performed with SNAP 4DX Plus® (IDEXX Laboratories, Fremont, CA) to reveal the presence of D. immitis antigens and the antibodies toward A. phagocytophilum and/or A. platys, E. canis and/or E. ewingii and B. burgdorferi.RESULTS: The results of this holistic approach show that all blood samples provided negative results for B. burgdorferi and E.canis/E. ewingii (0/1253), while 0.87% (11/1253) samples provided positive results for A. phagocytophilum/A. platys and 6.94% (87/1253) for D. immitis.CONCLUSION: The next studied topic would be to compare the results provided by the EIA technology with results of real time PCR and qPCR regarding these vector-borne pathogens.

scholarly journals Seroprevalence of Hepatitis E Virus Infection among Blood Donors in Bulgaria

Viruses ◽  
2021 ◽  
Vol 13 (3) ◽  
pp. 492
Author(s):  
Magdalena Baymakova ◽  
Krasimira Terzieva ◽  
Rumen Popov ◽  
Elisaveta Grancharova ◽  
Todor Kundurzhiev ◽  
...  
Keyword(s):  
Virus Infection ◽  
Hepatitis E Virus ◽  
Blood Donors ◽  
Hepatitis E ◽  
Elisa Test ◽  
Blood Samples ◽  
Wild Boars ◽  
Domestic Pigs ◽  
Specific Questionnaire ◽  
Positive Results

Hepatitis E virus (HEV) infection is widespread among domestic pigs, industrial swine, and wild boars in Bulgaria. The aim of the current research was to present the HEV seroprevalence among blood donors in Bulgaria. In the present study, 555 blood donors (479 males and 76 females) were enrolled from five districts in the country (Shumen, Pleven, Stara Zagora, Plovdiv, and Sofia districts). All blood samples were tested for anti-HEV IgG using the recomWell HEV IgG ELISA test (Mikrogen GmbH, Neuried, Germany). Each participating donor completed a short, structured, and specific questionnaire to document data on the current study. Anti-HEV IgG positive results were detected in 144 (25.9%) blood donors, including 129 (26.9%) males and 15 (19.7%) females. The established HEV seropositivity was 28.8% (23/80) in Shumen district, 23.2% (22/95) in Pleven district, 27.1% (38/140) in Stara Zagora district, 27.5% (44/160) in Plovdiv district, and 21.3% (17/80) in Sofia district. A high HEV seroprevalence was found for persons who declared that they were general hunters (48.7%; 19/39; p = 0.001) and hunters of wild boars (51.6%; 16/31; p = 0.001). We present the first seroprevalence rates of HEV infection in blood donors from Bulgaria. The results of our research showed high HEV seropositivity among blood donors.

scholarly journals Decline in prevalence and asymmetric distribution of human T cell lymphotropic virus 1 and 2 in blood donors, State of Minas Gerais, Brazil, 1993 to 2007

2010 ◽  
Vol 43 (6) ◽  
pp. 615-619 ◽  
Author(s):  
Maria Regina Dias-Bastos ◽  
Cláudia Di Lorenzo Oliveira ◽  
Anna Bárbara de Freitas Carneiro-Proietti
Keyword(s):  
T Cell ◽  
Odds Ratio ◽  
Blood Donors ◽  
Elisa Test ◽  
Minas Gerais ◽  
Asymmetric Distribution ◽  
Donor Pool ◽  
Human T Cell ◽  
Low Prevalence ◽  
Positive Results

INTRODUCTION: Human T cell lymphotropic virus types 1 and 2 (HTLV-1/2) are endemic in Brazil and are screened for in transfusion services since 1993. This study evaluated the evolution of the prevalence of HTLV-1 and 2 in blood donors of the Hemominas Foundation from 1993 to 2007, and its geographical distribution in State of Minas Gerais, Brazil. METHODS: The Hemominas Foundation is a centralized blood center in Minas Gerais, Brazil. The sources of data were the Hemominas Foundation Technical Bulletin and files from the centralized serological laboratory. Donors were tested in the period using enzyme linked immuno sorbent assays (ELISA), followed by Western blot, when repeatedly reactive. The data were analyzed by EPIINFO 6.2 and TABWIN 3.5 softwares. RESULTS: The average seroprevalence in the period 1993-2007 was 0.1%. A steady decline occurred from 0.4% in 1993 to below 0.1% in 2002 and later, with a transient peak of 0.5% in 1994. HTLV reactivity distribution was asymmetrical in the state, with regions of higher prevalence, interspersed with low prevalence areas. Comparison of positive and negative donors verified that increasing age was proportional to virus positivity. Odds ratio for age ranged from 1.43 (30 to 39 years-old) to 3.09 (50 to 65 years-old). Women had a greater chance of being positive (OR-1.64), as previously described. CONCLUSIONS: Possible explanations for HTLV-1/2 prevalence decline are the exclusion of positive donors from the donor pool, an increase in repeat donors and ELISA test improvement, with reduction in the number of false positive results.

scholarly journals Short Communication: Molecular characterization and blood hematology profile of dogs infected by Ehrlichia canis in Yogyakarta, Indonesia

2020 ◽  
Vol 21 (7) ◽  
Author(s):  
Yustinus oswin primajuni Wuhan ◽  
Aris Haryanto ◽  
Ida Tjahajati
Keyword(s):  
Molecular Characterization ◽  
Microscopic Examination ◽  
Short Communication ◽  
Pcr Amplification ◽  
Ehrlichia Canis ◽  
Infected Blood ◽  
Blood Samples ◽  
Glta Gene ◽  
Vector Borne ◽  
Positive Results

Abstract. Wuhan YOP, Haryanto A, Tjahajati I. 2020. Short Communication: Molecular characterization and blood hematology profile of dogs infected by Ehrlichia canis in Yogyakarta, Indonesia. Biodiversitas 21: 3242-3248. Ehrlichia canis is Gram-negative intracellular obligate bacteria that cause ehrlichiosis, a companion vector-borne disease is a potentially fatal disease that attacks dogs. The purpose of this study was to molecular characterize and determine the features of Ehrlichia-infected blood based on the amplification of the gltA gene in Ehrlichia infected dogs from Yogyakarta, Indonesia. Blood samples were collected from 51 dog patients from the Prof. Dr. Soeparwi Animal Hospital, animal clinics, and pet shops based on the anamnesis, clinical sign, and physical examination, followed by microscopic examination, routine hematology, PCR amplification, and DNA sequencing were carried out on the blood samples. Based on positive PCR amplification and blood hematology profile examination ehrlichiosis-positive in dogs showed that thrombocytopenia case was 82.3%, anemia was 70.5%, eosinopenia was 70.5%, neutropenia was 29.4%, monocytopenia was 23%, leukopenia was 17% and lymphopenia was 11.7%. Morulae of Ehrlichia sp.was not found in microscopic examination. Molecularly, detected of E. canis using the gltA gene showed that 34% of samples were positive results. Then 5 of positive Ehrlichia samples were DNA sequenced, they showed a high homology of 100% with Hat Yai isolates (KU765199.1). There was no genetic diversity between E. canis samples in Yogyakarta.

scholarly journals Design of Data Mining Techniques for Online Blood Bank Management by CNN Model

2021 ◽  
Vol 3 (3) ◽  
pp. 213-228
Author(s):  
I. Jeena Jacob ◽  
P. Ebby Darney
Keyword(s):  
Machine Learning ◽  
Blood Donors ◽  
Blood Pressure Level ◽  
Blood Bank ◽  
Machine Learning Algorithms ◽  
Machine Learning Techniques ◽  
Model Classification ◽  
Bank Management ◽  
Learning Techniques ◽  
Blood Banks

A blood bank is the organisation responsible for storing blood to transfuse it to the patients in need. The primary goal of a blood bank is to be reliable and ensure that patients get the relevant non-toxic blood to avoid transfusion-related complications since blood is a critical medicinal resource. It is difficult for the blood banks to offer high levels of precision, dependability, and automation in the blood storage and transfusion process if blood bank administration includes many human processes. This research framework is proposing to maintain blood bank records using CNN model classification method. In the pre-processing of CNN method, the datasets are tokenized and set the donor’s eligibility. It will make it easier for regular blood donors to donate regularly to charitable people and organizations. A few machine learning techniques offer the automated website updation. Jupyter note book has been used to analyze the dataset of blood donors using decision trees, neural networks, and von Bays techniques. The proposed method operates online through a website. Moreover, the donor's eligibility status with gender, body mass index, blood pressure level, and frequency of blood donations is also maintained. Finally, the comparison of different machine learning algorithms with the suggested framework is tabulated.

scholarly journals Human T-cell lymphotropic virus types 1 and 2 antibodies seroprevalence among blood donors at national public health blood bank, Khartoum- Sudan 2019

2020 ◽  
Vol 8 (3) ◽  
pp. 100-104
Author(s):  
Goris BMT
Keyword(s):  
Public Health ◽  
T Cell ◽  
Blood Donors ◽  
Blood Bank ◽  
Public Health Laboratory ◽  
Blood Samples ◽  
Adult T Cell Leukemia ◽  
Human T Cell ◽  
Result Show ◽  
National Public Health

The human T-cell lymphotropic virus types 1 and 2 (HTLV 1/2) are well known causes of adult T cell leukemia lymphoma. Both viruses were established to be transmitted through various mode including sexual contact and blood transfusion. This study was aimed to determine the seroprevalence of HTLV-1/2 antibodies among blood donors in Public Health Laboratory. During the period of August 2019 to October 2019 a total of 394 blood samples were obtained from blood donors visiting the Blood bank of National Public Health Laboratory, both males and females were included. The blood samples were analyzed for the presence of anti - HTLV-1,2 by a commercially available enzyme-linked immune-sorbent assay following the instructions of the manufacturer. The study participants were included 361 (91.6%) males and 33 (8.4%) females. The result show that of the 394 blood donors, four (4) were found to be seropositive for HTLV-1 antibodies giving a prevalence of 1.02%. While all samples were negative for the HTLV-2 antibodies. Among HTLV-1 positive cases 3 were male (75%) while only one female (25%) was found to be seropositive for HTLV-1 antibodies. None of the married donors was found to be seropositive for HTLV-1. We conclude that the seroprevalence of HTLV 1/2 were matched to the internationally estimated prevalence among blood donors at Blood Bank of national health laboratory and the majority of the cases were male under 40 years old. Further studies should be done with inclusion of more samples and using more sensitive technique like Western blot or PCR.

scholarly journals Seroprevalence of markers of transfusiontransmissible diseases in positive blood donors detected in routine screening in the Blood Bank of Hospital Carlos Luis Valverde Vega, Costa Rica. Period 2015 – 2019

2021 ◽  
Vol 9 (4) ◽  
pp. 70-73
Author(s):  
Jean Pablo Castro-Ruiz
Keyword(s):  
Infectious Diseases ◽  
Blood Donors ◽  
Blood Donation ◽  
Individual Characteristics ◽  
Blood Bank ◽  
Health Centers ◽  
Blood Components ◽  
Blood Banks ◽  
Scientific Ethics ◽  
The Individual

Blood is one of the fundamental tissues of our body. Blood donation allows blood banks to meet hospital needs for blood components. Blood transfusion is an activity that carries a risk, originated by a series of causal actions derived from the individual characteristics of donors and patients. Blood components are biological products and as such, they can never be exempt from the risk of transmitting infectious diseases, such as hepatitis B, HIV, syphilis, among others. Thanks to the investigation of the donor database of the Blood Bank of Hospital Dr. Carlos Luis Valverde Vega and the annual statistics of the blood bank, the statistics referring to donations were obtained, according to the type, grade of acceptance and as well as the prevalence of mandatory screening infectious diseases in the blood bank, between the years 2015 and 2019, with the respective authorization of the corresponding scientific ethics committee. This study reported a prevalence for Anti HB Core of 0.99%, 0.14% for HBsAg, 0.11% for syphilis, 0.05% for hepatitis C, 0.02% for HIV and 0% for the disease de Chagas and HTLV I / II. By using the bases of this work, future analyzes with a similar premise may be developed in different blood banks of the different health centers in our country.

scholarly journals Hepatitis B and Malaria Among Nepalese Blood Donors

1970 ◽  
Vol 5 (5) ◽  
pp. 81-84 ◽  
Author(s):  
Prakash Ghimire ◽  
Bishnu Bhakta Dhungyel ◽  
Bishnu Raj Tiwari
Keyword(s):  
Hepatitis B ◽  
Viral Hepatitis ◽  
Surface Antigen ◽  
Disease Burden ◽  
Blood Donors ◽  
Hepatitis B Surface Antigen ◽  
Malarial Parasite ◽  
Blood Samples ◽  
Blood Banks ◽  
Donated Blood

Viral hepatitis and malaria, both are the diseases with noticeable disease burden in Nepal. Malaria is seasonal with high disease burden during post rainy season. Severe malaria also shows similar symptoms to viral hepatitis. This is collaborative study has been conducted during June - September 2006 with the objective of determining the prevalence of hepatitis B and malaria in Nepalese blood donors to find out the need of routine malaria testing in each pint of donated blood. During the study period, screening of malaria and hepatitis B surface antigen (HBsAg) were done in 1200 blood samples collected from blood donors at Kathmandu, Nepalgunj and Biratnagar Blood Banks. Malaria diagnosis was done using one drop of blood from each unit of blood using Giemsa stained thick and thin smear microscopy, while hepatitis B surface antigen (HBsAg) was detected using commercial ELISA. Of the total 1200 blood samples analyzed, 600 were collected at Kathmandu and 300 each at Nepalgunj and Biratnagar blood banks, situated in malaria endemic southern districts. Among the total 1200 samples, 1% (12) of the sample was found reactive for HBsAg; while only 0.33 % (4) samples were positive for malarial parasite. 1.33% (8) samples from Kathmandu and 1.33% (4) samples from Nepalgunj were positive for HBV. 1% (3) samples from Nepalgunj and 0.33% (1) sample from Biratnagar were found to be positive for malarial parasite. None of the samples from Biratnagar were positive for HBsAg, while none of the samples from Kathmandu were positive for malarial parasite. All of the malaria cases were due to Plasmodium vivax. All the infected cases were male. Co-infection of malaria and HBV was not observed during the entire period of study. However more extensive study is needed in other endemic areas of Nepal, the study indicated that the continuation of screening each point of donated blood for Hepatitis B, while screening of malarial parasites in donors of endemic terai districts may be useful in preventing transmission of malaria through transfusion. Key words: Hepatitis; Malaria; ELISA; Blood donors; Blood transfusion. DOI: 10.3126/sw.v5i5.2661 Scientific World, Vol. 5, No. 5, July 2007 81-84

scholarly journals Demonstrating the presence of Ehrlichia canis DNA from different tissues of dogs with suspected subclinical ehrlichiosis

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Carlos A. Rodríguez-Alarcón ◽  
Diana M. Beristain-Ruiz ◽  
Angélica Olivares-Muñoz ◽  
Andrés Quezada-Casasola ◽  
Federico Pérez-Casio ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Lymph Node ◽  
Cross Reactivity ◽  
Ehrlichia Canis ◽  
Serological Tests ◽  
Blood Samples ◽  
Negative Results ◽  
Target Organs ◽  
Polymerase Chain ◽  
Positive Results

Abstract Background Nowadays, Ehrlichia canis receives increasing attention because of its great morbidity and mortality in animals. Dogs in the subclinical and chronic phases can be asymptomatic, and serological tests show cross-reactivity and fail to differentiate between current and past infections. Moreover, there could be low parasitaemia, and E. canis might be found only in target organs, hence causing results to be negative by polymerase chain reaction (PCR) on blood samples. Methods We evaluated by PCR the prevalence of E. canis in blood, liver, spleen, lymph node and bone marrow samples of 59 recently euthanised dogs that had ticks but were clinically healthy. Results In total, 52.55% of the blood PCRs for E. canis were negative, yet 61.30% yielded positive results from tissue biopsies and were as follows: 63.15% from bone marrow; 52.63% from liver; 47.36% from spleen; and 15.78% from lymph node. In addition, 33% had infection in three tissues (spleen, liver and bone marrow). Conclusions Our results show the prevalence of E. canis from tissues of dogs that were negative by blood PCR. Ehrlichia canis DNA in tissue was 30% lower in dogs that tested negative in PCR of blood samples compared to those that were positive. However, it must be taken into account that some dogs with negative results were positive for E. canis in other tissues.

A PCR survey of vector-borne pathogens in different dog populations from Turkey

2017 ◽  
Vol 62 (3) ◽  
Author(s):  
Huanping Guo ◽  
Ferda Sevinc ◽  
Onur Ceylan ◽  
Mutlu Sevinc ◽  
Ege Ince ◽  
...  
Keyword(s):  
Blood Samples ◽  
Pet Dogs ◽  
Specific Pcr ◽  
Vector Borne ◽  
Positive Results ◽  
Substantial Morbidity

AbstractIn the present study, a total of 192 blood samples were collected from pet dogs, kennel dogs and shepherd dogs in Konya district, Turkey, and tested by specific PCR for the presence of vector-borne pathogens. Several pathogens were identified, most of which can cause substantial morbidity in dogs. PCR results revealed that 54 (28.1%) dogs were infected with one or more pathogens. Positive results were obtained for

scholarly journals Demonstrating the presence of Ehrlichia canis DNA from different tissues of dogs with suspected subclinical ehrlichiosis

2020 ◽  
Author(s):  
Carlos Arturo Rodríguez-Alarcón ◽  
Diana M. Beristain-Ruiz ◽  
Angélica Olivares-Muñoz ◽  
Andrés Quezada-Casasola ◽  
Federico Pérez-Casio ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Cross Reactivity ◽  
Ehrlichia Canis ◽  
Morbidity And Mortality ◽  
Blood Samples ◽  
Serologic Tests ◽  
Negative Results ◽  
Target Organs ◽  
Positive Results ◽  
Different Tissues

Abstract Background: Nowadays, Ehrlichia canis receives more attention because of its great morbidity and mortality in animals. Dogs in the subclinical and chronic phases can be asymptomatic, and serologic tests show cross-reactivity and fail to differentiate between current and past infections. Moreover, there could be low parasitaemia, and E. canis might be found only in target organs, hence negative by PCR in blood. Methods: We evaluated by PCR the prevalence of E. canis in blood, liver, spleen, lymphatic nodules, and bone marrow in 59 recently euthanized dogs that had ticks but were clinically healthy. Results: In total, 52.55% of the blood PCRs for E. canis were negative, yet 61.30% yield positive results in tissue biopsies as follows: 63.15% from bone marrow, 52.63% from liver, 47.36% from spleen and 15.78% from lymphatic nodules. In addition, 33% had infection in three tissues (spleen, liver and bone marrow). Conclusions: Our results show prevalence of E. canis in tissue from dogs that were negative by PCR in blood. E. canis DNA in tissue was 30% lower in dogs that tested negative in blood samples by PCR, compared to those that were positive. However, it must be taken into account that some dogs with negative results were positive for E. canis in others tissues.

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