scholarly journals POST-MORTEM INTERVAL ESTIMATION USING POLYCRYSTALLINE CEREBROSPINAL FLUID FILMS TWO-DIMENSIONAL CARTOGRAPHY OF MUTUAL LASER POLARIZATION DISTRIBUTION LEVELS

2018 ◽  
Vol 6 ◽  
pp. 892-897
Author(s):  
Marta Garazdiuk ◽  
Oleh Wanchuliak ◽  
Oleksandr Pavlyukovich ◽  
Natalia Pavlyukovich ◽  
Oleksandr Garazdiuk
Keyword(s):  
Cerebrospinal Fluid ◽  
Large Scale ◽  
Interval Estimation ◽  
Diagnostic Methods ◽  
Two Dimensional ◽  
Post Mortem ◽  
Polycrystalline Films ◽  
Fluid Films ◽  
Microscopic Images ◽  
Post Mortem Interval

Post-mortem interval estimation is one of the most important issues in forensic practice. Optical diagnostic methods of biological tissue structure assessment are perspective in this area.The objects of investigation are polycrystalline films of cerebrospinal fluid, taken from 64 corpses with accurately known times of death and 15 healthy volunteers.The method of two-dimensional stokes-polarimetric mapping of distributions of a complex degree of mutual polarization with spatial-frequency filtration of microscopic images of cerebrospinal fluid films in the time monitoring of post-mortem changes in optical manifestations of polycrystalline networks has been tested in order to estimate the post-mortem interval. The most sensitive post-mortem changes in the optical manifestations of polycrystalline cerebrospinal fluid networks are revealed - statistical moments of the third and fourth orders that characterize the asymmetry and the excess (severity of the peak) of the distributions of values of the complex degree of mutual polarization of large-scale components of cerebrospinal fluid polycrystalline films microscopic images.An interval of 48 hours with the accuracy of the post-mortem interval estimation in ± 30 minutes were established by the method of two-dimensional mapping of distributions of the values of a complex degree of mutual polarization of large-scale component of cerebrospinal fluid films microscopic images.

scholarly journals Post-mortem interval estimation by laser-induced fluorescence of polycrystalline cerebro-spinal fluid films images

2016 ◽  
pp. 32-38
Author(s):  
Marta Garazdiuk
Keyword(s):  
Short Range ◽  
Interval Estimation ◽  
Statistical Processing ◽  
Laser Induced Fluorescence ◽  
Diagnostic Methods ◽  
Postmortem Changes ◽  
Two Dimensional ◽  
Post Mortem ◽  
Fluid Films ◽  
Post Mortem Interval

There are many new optical diagnostic methods (photometric, polarization and correlation techniques) of biological tissue structure assessment, which are the most perspective in post-mortem interval determination. The purpose of the work. Development and testing of the two-dimensional Stokes-polarimetric mapping of biological layers own fluorescence to evaluate accuracy of the post-mortem interval (PMI) assessment using statistical analysis of postmortem changes dynamics of the coordinate distributions values of polycrystalline films of liquor (PFL) images laser-induced fluorescence polarization intensity (LIFPI). Materials and methods. Objects of investigation are PFL, taken in 72 corpses with accurately known time of death and 20 healthy volunteers. The cause of the death was cardiovascular accident. Coordinate distributions of LIFPI image values were determined for each sample of PFL in the optical arrangement of the Stokes polarimeter in short-range spectrum of optical radiation. The value of statistical points 1 - 4 th order was perfomed for each two-dimensional distribution of PFL images LIFPI values. Statistical processing of the calculated values of set of points that characterize the LIFPI distributions was carry out. The depending on the time change of the most sensitive points of statistical values were built. Results and conclusions. Two-dimensional Stokes-polarimetric mapping distributions LIFPI of PFL images may be used in determination of the PMI. Statistical points of the first and fourth order are the most sensitive PFL optical values to evaluate postmortem changes by short-range fluorescence.

scholarly journals COMPARATIVE ANALYSIS OF LASER POLARIMETRY METHODS OF POLYCRYSTALLINE FILMS OF CEREBROSPINAL FLUID FOR POST-MORTEM INTERVAL ESTIMATION

2019 ◽  
Vol 7 ◽  
Author(s):  
Marta Garazdiuk ◽  
Viktor Bachynskіy ◽  
Oleh Vanchuliak ◽  
Oleksandr Garazdiuk ◽  
Yuliya Sarkisova
Keyword(s):  
Cerebrospinal Fluid ◽  
Interval Estimation ◽  
Accurate Estimation ◽  
Time Interval ◽  
Two Dimensional ◽  
Post Mortem ◽  
Polycrystalline Films ◽  
Technological Support ◽  
Post Mortem Interval ◽  
Stokes Polarimetry

Obtaining the most accurate information about an object during its evaluation is one of the most urgent tasks of current forensic medical examination. That is why the application of new technologies in forensic medical expertise becomes an integral part of the technological support of forensic examination institutions. Purpose: To compare the effectiveness of cerebrospinal fluid polycrystalline films, microscopic images, two-dimensional Stokes-polarimetry, and autofluorescent polarimetry in post-mortem interval estimation over long- and short-term time intervals. Material and methods. The object of the study is cerebrospinal fluid donated from 70 corpses (experimental group) and 20 living persons (control group). Methods used: two-dimensional Stokes-polarimetry, autofluorescent polarimetry. Results: Quantitative characteristics of the cerebrospinal fluid’s polycrystalline films statistical moments allows us to establish relations between them and the duration of the post-mortem interval at a significant time interval. However, this method does not consider the morphology of the film of the cerebrospinal fluid and its coordinate structure. In contrast to this method, fluorescence microscopy considers the biochemical composition of the biological fluid and carries information about the concentration of molecular complexes of proteins, NADH, flavins, porphyrins, etc. In the early post-mortem period, changes in the cerebrospinal fluid begin precisely with changes in the concentration of biochemical compounds, and crystalline changes are secondary, so it can be argued that this method is most effective for diagnosing the progression of the time in the first hours after death. Conclusions: Fluorescent laser polarimetric methods allow accurate estimation of the postmortem interval during the first hours after death.  While, the polarization methods is more effective during the long-term periods of time, but has less precision.

scholarly journals POST-MORTEM INTERVAL ESTIMATION BY CEREBRO-SPINAL FLUID FILMS POLARIZATION IMAGES OPTICAL STRUCTURE CHANGING

2016 ◽  
Vol 4 ◽  
pp. 711-718
Author(s):  
Marta Garazdiuk ◽  
Viktor Bachynskіy ◽  
Oleg Wanchuliak ◽  
Oleksandr Garazdiuk ◽  
Aleksandr Ushenko
Keyword(s):  
Optical Networks ◽  
Interval Estimation ◽  
Two Dimensional ◽  
Post Mortem ◽  
Polycrystalline Films ◽  
Fluid Films ◽  
Post Mortem Interval ◽  
Optimal Interval ◽  
Optical Structure ◽  
Pmi Estimation

INTRODUCTION: Post-mortem interval (PMI) estimation has been and remains one of the main issues of forensic medicine. Complex photometric, polarization, and correlation techniques of biological tissue (BT) structure are crucial in this area.OBJECTIVES: The purpose of this study is to develop and test two-dimensional Stokes-polarimetric mapping of biological layers to evaluate the accuracy of PMI assessments using statistical analysis of post-mortem changes and dynamics of the coordinate distributions for the polarization ellipticity (PE) images of polycrystalline films of liquor (PFL). Coordinate distributions of polarization ellipticity (PE) image values were estimated for each sample of PFL in the optical arrangement of the Stokes polarimeter using the method of two-dimensional distributions of Stokes-parameters.RESULTS: The most sensitive statistics to the post-mortem manifestations in terms of changes to liquor polycrystalline optical networks were those for the 3rd and 4th order. They characterized asymmetry and kurtosis (peak sharpness) of coordinate distributions for polarization ellipticity images of polycrystalline films of liquor. The optimal interval was 32 hours, with accuracy of 60 min, from the method of two-dimensional Stokes-polarimetric mapping used.

A novel method for post-mortem interval estimation based on tissue nano-mechanics

2019 ◽  
Vol 133 (4) ◽  
pp. 1133-1139 ◽  
Author(s):  
Fabio De-Giorgio ◽  
Matteo Nardini ◽  
Federica Foti ◽  
Eleonora Minelli ◽  
Massimiliano Papi ◽  
...  
Keyword(s):  
Interval Estimation ◽  
Post Mortem ◽  
Post Mortem Interval ◽  
Novel Method

Post-mortem interval estimation based on insect evidence in a quasi-indoor habitat

2019 ◽  
Vol 59 (1) ◽  
pp. 109-115 ◽  
Author(s):  
Szymon Matuszewski ◽  
Anna Mądra-Bielewicz
Keyword(s):  
Interval Estimation ◽  
Post Mortem ◽  
Post Mortem Interval

Estimation of post-mortem interval: A comparison between cerebrospinal fluid and vitreous humour chemistry

2015 ◽  
Vol 36 ◽  
pp. 144-148 ◽  
Author(s):  
Rajanikanta Swain ◽  
Adarsh Kumar ◽  
Jyotiranjan Sahoo ◽  
R. Lakshmy ◽  
S.K. Gupta ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Vitreous Humour ◽  
Post Mortem ◽  
Post Mortem Interval

scholarly journals The development of a post-mortem interval estimation for human remains found on land in the Netherlands

2017 ◽  
Vol 132 (3) ◽  
pp. 863-873 ◽  
Author(s):  
H. T. Gelderman ◽  
L. Boer ◽  
T. Naujocks ◽  
A. C. M. IJzermans ◽  
W. L. J. M. Duijst
Keyword(s):  
The Netherlands ◽  
Interval Estimation ◽  
Human Remains ◽  
Post Mortem ◽  
Post Mortem Interval
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