scholarly journals Functional Monomers and Polymers CLXXVIII. Specific Interaction of Nucleic Acid Analogs in HPLC System

1989 ◽  
Vol 21 (5) ◽  
pp. 425-432 ◽  
Author(s):  
Suguru Nagae ◽  
Yoshiaki Inaki ◽  
Kiichi Takemoto
Keyword(s):  
Nucleic Acid ◽  
Specific Interaction ◽  
Functional Monomers ◽  
Nucleic Acid Analogs ◽  
Hplc System

Nucleic Acid Analogs: Their Specific Interaction and Applicability

1990 ◽  
pp. 253-267 ◽  
Author(s):  
Kiichi Takemoto ◽  
Eiko Mochizuki ◽  
Takehiko Wada ◽  
Yoshiaki Inaki
Keyword(s):  
Nucleic Acid ◽  
Specific Interaction ◽  
Nucleic Acid Analogs

Synthesis and Interaction of Water Soluble Nucleic Acid Analogs

1991 ◽  
pp. 31-45 ◽  
Author(s):  
Kiichi Takemoto ◽  
Takehiko Wada ◽  
Eiko Mochizuki ◽  
Yoshiaki Inaki
Keyword(s):  
Nucleic Acid ◽  
Water Soluble ◽  
Nucleic Acid Analogs

Chicken histone H5 inhibits transcription and replication when introduced into proliferating cells by microinjection

1988 ◽  
Vol 91 (2) ◽  
pp. 201-209
Author(s):  
M.G. Bergman ◽  
E. Wawra ◽  
M. Winge
Keyword(s):  
Nucleic Acid ◽  
Natural Environment ◽  
Specific Interaction ◽  
Condensed State ◽  
Pulse Labelling ◽  
Chicken Erythrocyte ◽  
Biological Functions ◽  
Proliferating Cells ◽  
Histone H5 ◽  
Transcription And Replication

Chicken erythrocyte histone H5 has been suggested repeatedly to be a general suppressor of transcription and replication. Therefore, the biological functions of H5 were investigated and compared with those of H1 (H1a + H1b) by microinjection of the purified proteins into proliferating L6 rat myoblasts. By pulse-labelling of the injected cells with [3H]uridine and [3H]thymidine it was shown that H5 blocked both transcription and replication substantially, and that the chromatin of the injected cells became densely compacted. H1 also suppressed these functions, but to a much lesser degree. The effects were specific and not caused by change in intracellular pH caused by introduction of the very basic H5, or its non-specific interaction with nucleic acid, since injection of protamine or lysozyme did not affect the cells. The migration and localization of injected H5 was monitored at different times after injection by immunofluorescence, which revealed that H5 was efficiently and stably concentrated in the nucleus. The results indicate that H5 indeed might function as an inactivator of the erythroid genome in its natural environment, probably by keeping the chromatin in a very condensed state.

scholarly journals Role of Lipid-Based and Polymer-Based Non-Viral Vectors in Nucleic Acid Delivery for Next-Generation Gene Therapy

Molecules ◽  
2020 ◽  
Vol 25 (12) ◽  
pp. 2866 ◽  
Author(s):  
Aniket Wahane ◽  
Akaash Waghmode ◽  
Alexander Kapphahn ◽  
Karishma Dhuri ◽  
Anisha Gupta ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Nucleic Acid ◽  
Messenger Rna ◽  
Viral Vectors ◽  
Cationic Lipids ◽  
Nucleic Acid Delivery ◽  
Nucleic Acid Analogs ◽  
Organ Specific ◽  
Interfering Rna

The field of gene therapy has experienced an insurgence of attention for its widespread ability to regulate gene expression by targeting genomic DNA, messenger RNA, microRNA, and short-interfering RNA for treating malignant and non-malignant disorders. Numerous nucleic acid analogs have been developed to target coding or non-coding sequences of the human genome for gene regulation. However, broader clinical applications of nucleic acid analogs have been limited due to their poor cell or organ-specific delivery. To resolve these issues, non-viral vectors based on nanoparticles, liposomes, and polyplexes have been developed to date. This review is centered on non-viral vectors mainly comprising of cationic lipids and polymers for nucleic acid-based delivery for numerous gene therapy-based applications.

scholarly journals Use of Nucleic Acid Analogs for the Study of Nucleic Acid Interactions

2011 ◽  
Vol 2011 ◽  
pp. 1-11 ◽  
Author(s):  
Shu-ichi Nakano ◽  
Masayuki Fujii ◽  
Naoki Sugimoto
Keyword(s):  
Nucleic Acid ◽  
Base Pair ◽  
Double Helix ◽  
Nucleoside Analogs ◽  
Structural Analog ◽  
Base Pairs ◽  
Nucleic Acid Analogs ◽  
Dna Double Helix ◽  
Nucleic Acid Interactions ◽  
Site Selective

Unnatural nucleosides have been explored to expand the properties and the applications of oligonucleotides. This paper briefly summarizes nucleic acid analogs in which the base is modified or replaced by an unnatural stacking group for the study of nucleic acid interactions. We also describe the nucleoside analogs of a base pair-mimic structure that we have examined. Although the base pair-mimic nucleosides possess a simplified stacking moiety of a phenyl or naphthyl group, they can be used as a structural analog of Watson-Crick base pairs. Remarkably, they can adopt two different conformations responding to their interaction energies, and one of them is the stacking conformation of the nonpolar aromatic group causing the site-selective flipping of the opposite base in a DNA double helix. The base pair-mimic nucleosides can be used to study the mechanism responsible for the base stacking and the flipping of bases out of a nucleic acid duplex.

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