scholarly journals Bioaccumulation Kinetics and Bio Concentration Factors of Few Polychlorinated Biphenyls: A Study on Freshwater Fish Puntius ticto

2020 ◽  
pp. 279-289
Author(s):  
Advait Bhagade
Keyword(s):  
Polychlorinated Biphenyls ◽  
Exposure Time ◽  
Gill Tissue ◽  
Hydrophobic Property ◽  
Time Prediction ◽  
Pcb 126 ◽  
Water Partition Coefficient ◽  
Wet Weight ◽  
Concentration Factors ◽  
Model Fish

The present work aims to understand the bioaccumulation kinetics and bioconcentration factors (BCF) of the Polychlorinated Biphenyls (PCBs) PCB 126 and PCB 169 in tissues of fish Puntius ticto. A continuous fed system was used to carry out the study. Bioconcentration in the tissues of the fish has been summarised using a first order uptake model. Fish were exposed to the PCBs continuously for a period of 30 days and this exposure has been used to calculate the steady state of BCF. The gill tissue of P. ticto showed a maximum rate of bioaccumulation of 30.61 μg g-1 wet weight for PCB 169 and 19.91 μg g-1 wet weight for PCB 126 respectively. In this study, the regression coefficients (R2), were found to vary between 0.736 and 0.97. This is indicative of a good to high correlation between the PCB concentration and exposure time. Prediction of the Octanol water partition coefficient (Kow) has been carried out to demonstrate the hydrophobic property of PCB compounds and their attraction towards lipid. The actual calculated BCF values have been used to predict the Kow values. Results indicate that it is possible to correlate the Kow values with both the lipid content of the tissue and the exposure time, in case of either PCB, and that the burden of PCB differs from one tissue to another.

Polychlorinated Biphenyls PCB 153 and PCB 126 Impair the Glutamate–Nitric Oxide–cGMP Pathway in Cerebellar Neurons in Culture by Different Mechanisms

2009 ◽  
Vol 16 (2) ◽  
pp. 97-105 ◽  
Author(s):  
Marta Llansola ◽  
Blanca Piedrafita ◽  
Regina Rodrigo ◽  
Carmina Montoliu ◽  
Vicente Felipo
Keyword(s):  
Nitric Oxide ◽  
Polychlorinated Biphenyls ◽  
Cerebellar Neurons ◽  
Pcb 126 ◽  
Cgmp Pathway

scholarly journals Influence of polychlorinated biphenyls (PCBs) and phytoestrogens on prostaglandin F2α and E2secretion from bovine endometrial cells at a postovulatory stage of the estrous cycle 

2012 ◽  
Vol 50 (No. 11) ◽  
pp. 487-495 ◽  
Author(s):  
M. Wrobel ◽  
J. Kotwica
Keyword(s):  
Polychlorinated Biphenyls ◽  
Estrous Cycle ◽  
Oestrous Cycle ◽  
Uterine Contractility ◽  
Endometrial Cells ◽  
Aroclor 1248 ◽  
Pcb 126 ◽  
Prostaglandin F

Polychlorinated biphenyls (PCBs) and phytoestrogens were found to affect contractions of bovine uterus. Prostaglandins (PG) F<sub>2</sub><sub>&aacute;</sub> and E<sub>2</sub> are also involved in the uterine contractility. Hence the aim of these studies was to investigate the effect of PCBs and some phytoestrogens on PG secretion from endometrial cells obtained on days 1&ndash;5 of the oestrous cycle. Cells were incubated in aerated atmosphere at 38&deg;C for 24 h, separately with the mixture of PCBs &ndash; Aroclor 1248 (10 ng/ml), with individual congeners -77, -126 or -153 (each at the dose 100 &nbsp;g/ml), coumestrol, daidzein or genistein (each at the dose 10<sup>&ndash;6</sup>M) or jointly each PCB with each of the phytoestrogens. Using the TOX1-kit neither Aroclor 1248 (Ar 1248) nor individual congeners were found to affect the viability of cells compared to the control (P &gt; 0.05). All used PCBs markedly increased the metabolite of PGF<sub>2</sub><sub>&aacute;</sub>(PGFM) concentrations (P &lt; 0.05) but not PGE<sub>2 </sub>(P &gt; 0.05). Hence the ratio of PGF<sub>2</sub><sub>&aacute;</sub> to PGE<sub>2</sub> was also increased by PCBs. However, when these cells were incubated with each of the phytoestrogens, there was a decrease in both PGF<sub>2</sub><sub>&aacute;</sub> and PGE<sub>2</sub> secretion compared to the control (P &lt; 0.05) but without altering the PGF<sub>2</sub><sub>&aacute;</sub> : PGE<sub>2</sub> ratio. Moreover, phytoestrogens could clearly reduce the concentrations of PGFM elicited by PCBs, and they reduced PGE<sub>2</sub> secretion compared to that evoked by PCB-126 and &ndash;153 only. Thus phytoestrogens can restore the proper ratio of PGF<sub>2</sub><sub>&aacute;</sub> : PGE<sub>2</sub> secreted by the bovine endometrium.

scholarly journals Effect of polychlorinated biphenyls on the secretion of oxytocin from luteal and granulosa cells in cow: possible involvement of glucocorticoid receptors

2012 ◽  
Vol 51 (No. 7) ◽  
pp. 391-398
Author(s):  
Mlynarczuk JJ ◽  
J. Kotwica
Keyword(s):  
Polychlorinated Biphenyls ◽  
Granulosa Cells ◽  
Glucocorticoid Receptors ◽  
The Other ◽  
Luteal Cells ◽  
Pcb Congeners ◽  
Ovarian Cells ◽  
Affect Cell Viability ◽  
Pcb 126 ◽  
The One

Polychlorinated biphenyls (PCBs) stimulate oxytocin secretion from bovine granulosa and luteal cells. Since oxytocin on the one hand is released from ovarian cells by cortisol and on the other hand PCBs can be bound by glucocorticoid receptors (GCr), we have tested the hypothesis that PCBs acting via GCr can stimulate oxytocin secretion. In preliminary studies the effect of RU486 (GCr blocker) on cells viability was tested. Thereafter, a selected dose of RU486 (10<sup>5</sup>M), which did not affect cell viability, was used in further experiments. It appears that this dose of RU486 completely blocked GCr against cortisol-stimulated oxytocin secretion, in both types of cells. Furthermore, granulosa cells (10<sup>5</sup>) from follicles of two sizes (&gt;1 cm &lt; in diameter) and luteal cells (10<sup>5</sup>) from day 5&ndash;10 of estrous cycle were incubated for 72 h with congeners of PCB (126, 77 or 153) at doses of 1, 10 or 100&nbsp;ng/ml each, separately or jointly with RU486. The effect of PCB 77 and 153 on oxytocin secretion was blocked by RU486, but it did not change the effect evoked by PCB126 in both granulosa and luteal cells. We assume that some PCB congeners can affect oxytocin secretion from granulosa and luteal cells acting via GCr.

scholarly journals 3,3′,4,4′,5-Pentachlorobiphenyl Inhibits Drug Efflux Through P-Glycoprotein in KB-3 Cells Expressing Mutant Human P-Glycoprotein

2004 ◽  
Vol 2004 (3) ◽  
pp. 137-142 ◽  
Author(s):  
Hiroshi Fujise ◽  
Shigemi Sasawatari ◽  
Takeshi Annoura ◽  
Teruo Ikeda ◽  
Kazumitsu Ueda
Keyword(s):  
Amino Acid ◽  
Polychlorinated Biphenyls ◽  
Cyclosporine A ◽  
Drug Accumulation ◽  
Drug Efflux ◽  
Wild Type ◽  
P Glycoprotein ◽  
Pcb 126 ◽  
Coplanar Polychlorinated Biphenyls ◽  
Human Wild Type

The effects on the drug efflux of3,3′,4,4′,5-pentachlorobiphenyl (PCB-126), the most toxic of all coplanar polychlorinated biphenyls (Co-PCBs), were examined in KB-3 cells expressing human wild-type and mutant P-glycoprotein in which the 61st amino acid was substituted for serine or phenylalanine (KB3-Phe61). In the cells expressing P-glycoproteins, accumulations of vinblastine and colchicine decreased form 85% to 92% and from 62% to 91%, respectively, and the drug tolerances for these chemicals were increased. InKB3-Phe61, the decreases in drug accumulation were inhibited by adding PCB-126 in a way similar to that with cyclosporine A: by adding 1μM PCB-126, the accumulations of vinblastine and colchicine increased up to 3.3- and 2.3-fold, respectively. It is suggested that PCB-126 decreased the drug efflux by inhibiting the P-glycoprotein inKB3-Phe61. Since there were various P-glycoproteins and many congeners of Co-PCBs, this inhibition has to be considered a new cause of the toxic effects of Co-PCBs.

QSPR prediction of n-octanol/water partition coefficient for polychlorinated biphenyls

Chemosphere ◽  
2007 ◽  
Vol 69 (3) ◽  
pp. 469-478 ◽  
Author(s):  
Wenjuan Lü ◽  
Yonglei Chen ◽  
Mancang Liu ◽  
Xingguo Chen ◽  
Zhide Hu
Keyword(s):  
Polychlorinated Biphenyls ◽  
Partition Coefficient ◽  
Water Partition Coefficient

Polycyclic Aromatic Hydrocarbons, Polychlorinated Biphenyls, Chlorinated Pesticides (DDTs), Hexachlorocyclohexane, and Hexachlorobenzene Residues in Smoked Seafood

2003 ◽  
Vol 66 (6) ◽  
pp. 1095-1099 ◽  
Author(s):  
M. M. STORELLI ◽  
R. GIACOMINELLI STUFFLER ◽  
G. O. MARCOTRIGIANO
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Polychlorinated Biphenyls ◽  
Aromatic Hydrocarbons ◽  
The European Union ◽  
Chlorinated Pesticides ◽  
Smoked Fish ◽  
Food And Agriculture ◽  
Food And Agriculture Organization ◽  
Polycyclic Aromatic ◽  
Wet Weight

Smoked seafoods were screened for the presence of polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs), and other organochlorine compounds. Total PAH concentrations ranged from 46.5 ng/g (wet weight) for smoked swordfish to 124.0 ng/g (wet weight) for smoked herring. Among the carcinogenic PAHs, benzo(a)pyrene ranged from undetectable levels for several smoked fish to 0.7 ng/g for Scottish salmon, dibenzo(ah)anthracene was not present in any of the samples analyzed, and benzo(a)anthracene was found in all samples and at particularly high levels in salmon (23.2 ng/g). Benzo(a)pyrene concentrations were below the tolerance limit for all samples. PCB concentrations for the different samples ranged from 2 to 30 ng/g. Chlorinated pesticides (DDTs: p,p′-DDE, p,p′-DDT, o,p′-DDT, p,p′-DDD, and o,p′-DDD) were detected at levels ranging from 0.2 ng/g (wet weight) in bluefin tuna to 17.5 ng/g (wet weight) in salmon. Hexachlorocyclohexane isomers (αHCH + βHCH + γHCH) were present in higher amounts in eels (6.5 ng/g) than in the other smoked fish. For 40% of the samples, PCB concentrations exceeded the limit fixed by the European Union, while pesticide levels were below the maximum acceptable limit proposed by the Food and Agriculture Organization.

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