scholarly journals Isolation and Functional Characterization of Beneficial Bacteria Associated with Roots of Thysanolaena Maxima and Rhizospheric Soil Enzymatic Activities in Jhum Agriculture

2019 ◽  
Vol 7 (2) ◽  
pp. 189-200
Author(s):  
Juri Deka ◽  
Dwipendra Thakuria ◽  
Alarisa Khyllep ◽  
Giasuddin Ahmed
Keyword(s):  
Endophytic Bacteria ◽  
Organic Phosphorus ◽  
Biological Activities ◽  
Functional Characterization ◽  
Inorganic Phosphorus ◽  
Rhizospheric Soil ◽  
Pgp Traits ◽  
Plant Growth Promoting ◽  
Fallow Phase

The activity of amylase (AMY), arylsulphatase (ASA), β-glucosidase (GSA), dehydrogenase (DHA), acid-phosphomonoestarase (PHA) and protease (PRO) enzymes were analyzed in rhizospheric soils of broom grass, Thysanolaena maxima (TM) collected from fallow phases of 5 and 20 years Jhum cycles (F5 and F20, respectively) and their corresponding bulk soils. The activities of soil enzymes from rhizospheric soil of TMF5 were significantly higher relative to that of bulk soils and the rhizospheric soils from TMF20. The counts of rhizobacteria [0.74 ± 0.056 x 107 colony forming unit (cfu) g-1 soil] and root endophytic bacteria (0.083 ± 0.004 x 104 cfu g-1 roots) of TM from F20 fallow phase were higher compared to the counts of rhizobacteria and endophytic bacteria (0.27 ± 0.029 x 107cfu g-1 soil and 0.05 ± 0.008 x 104 cfu g-1 roots, respectively) of TM from F5 fallow phase. Altogether 63 isolates associated with TM were screened for multifaceted plant growth promoting (PGP) traits viz. production of pectinase and cellulase, IAA like substances, 1-aminocyclopropane-1-carboxylate deaminase (ACCD), N2-fixation, solubilisation of inorganic phosphorus (iP) from Ca3(PO4)2, AlPO4 and FePO4 and mineralization of organic phosphorus (Na-phytate). The PGP screening results indicated that the percent incidence of rhizobacteria and root endophytic bacteria for PGP traits was higher in F5 fallow phase as compared to F20 fallow phase. These results provided clear indication that TM plants play an important role in rejuvenating the biological activities (in terms of higher activities of enzymes in rhizospheric soils and greater population of beneficial rhizobacteria and root endophytes) in frequently burnt soils under shorter Jhum cycles.

scholarly journals Isolation and characterization of plant growth-promoting endophytic bacteria Bacillus stratosphericus LW-03 from Lilium wardii

3 Biotech ◽  
2020 ◽  
Vol 10 (7) ◽  
Author(s):  
Mohammad Sayyar Khan ◽  
Junlian Gao ◽  
Mingfang Zhang ◽  
Xuqing Chen ◽  
The Su Moe ◽  
...  
Keyword(s):  
Plant Growth ◽  
Endophytic Bacteria ◽  
Isolation And Characterization ◽  
Plant Growth Promoting ◽  
Growth Promoting

scholarly journals Characterization of Arsenic-Resistant Endophytic Bacteria From Alfalfa and Chickpea Plants

2021 ◽  
Vol 12 ◽  
Author(s):  
Hazhir Tashan ◽  
Behrouz Harighi ◽  
Jalal Rostamzadeh ◽  
Abdolbaset Azizi
Keyword(s):  
Nitrogen Fixation ◽  
Plant Growth ◽  
Endophytic Bacteria ◽  
Phosphate Solubilization ◽  
Lipase Production ◽  
Growth Potential ◽  
Sodium Arsenate ◽  
Plant Growth Promoting ◽  
Growth Promoting

The present investigation was carried out to isolate arsenic (As)-resistant endophytic bacteria from the roots of alfalfa and chickpea plants grown in arsenic-contamination soil, characterize their As tolerance ability, plant growth-promoting characteristics, and their role to induce As resistance by the plant. A total of four root endophytic bacteria were isolated from plants grown in As-contaminated soil (160–260-mg As kg−1 of soil). These isolates were studied for plant growth-promoting (PGP) characteristics through siderophore, phosphate solubilization, nitrogen fixation, protease, and lipase production, and the presence of the arsenate reductase (arsC) gene. Based on 16S rDNA sequence analysis, these isolates belong to the genera Acinetobacter, Pseudomonas, and Rahnella. All isolates were found As tolerant, of which one isolate, Pseudomonas sp. QNC1, showed the highest tolerance up to 350-mM concentration in the LB medium. All isolates exhibited phosphate solubilization activity. Siderophore production activity was shown by only Pseudomonas sp. QNC1, while nitrogen fixation activity was shown by only Rahnella sp. QNC2 isolate. Acinetobacter sp. QNA1, QNA2, and Rahnella sp. QNC2 exhibited lipase production, while only Pseudomonas sp. QNC1 was able to produce protease. The presence of the arsC gene was detected in all isolates. The effect of endophytic bacteria on biomass production of alfalfa and chickpea in five levels of arsenic concentrations (0-, 10-, 50-, 75-, and 100-mg kg−1 soil) was evaluated. The fresh and dry weights of roots of alfalfa and chickpea plants were decreased as the arsenic concentration of the soil was increased. Results indicate that the fresh and dry root weights of alfalfa and chickpea plants were significantly higher in endophytic bacteria-treated plants compared with non-treated plants. Inoculation of chickpea plants with Pseudomonas sp. QNC1 and Rahnella sp. QNC2 induced lower NPR3 gene expression in chickpea roots grown in soil with the final concentration of 100-mg kg−1 sodium arsenate compared with the non-endophyte-treated control. The same results were obtained in Acinetobacter sp. QNA2-treated alfalfa plants grown in the soil plus 50-mg kg−1 sodium arsenate. These results demonstrated that arsenic-resistant endophytic bacteria are potential candidates to enhance plant-growth promotion in As contamination soils. Characterization of bacterial endophytes with plant growth potential can help us apply them to improve plant yield under stress conditions.

scholarly journals Plant Growth Promotion Diversity in Switchgrass-Colonizing, Diazotrophic Endophytes

2021 ◽  
Vol 12 ◽  
Author(s):  
Sara Gushgari-Doyle ◽  
Marcus Schicklberger ◽  
Yifan V. Li ◽  
Robert Walker ◽  
Romy Chakraborty
Keyword(s):  
Plant Growth ◽  
Panicum Virgatum ◽  
Growth Promotion ◽  
Pgp Traits ◽  
Isolation And Characterization ◽  
Plant Growth Promoting ◽  
Cultivation Methods ◽  
Diazotrophic Endophytes ◽  
Raoultella Terrigena

Endophytic nitrogen-fixing (diazotrophic) bacteria are essential members of the microbiome of switchgrass (Panicum virgatum), considered to be an important commodity crop in bioenergy production. While endophytic diazotrophs are known to provide fixed atmospheric nitrogen to their host plant, there are many other plant growth-promoting (PGP) capabilities of these organisms to be demonstrated. The diversity of PGP traits across different taxa of switchgrass-colonizing endophytes is understudied, yet critical for understanding endophytic function and improving cultivation methods of important commodity crops. Here, we present the isolation and characterization of three diazotrophic endophytes: Azospirillum agricola R1C, Klebsiella variicola F10Cl, and Raoultella terrigena R1Gly. Strains R1C and F10Cl were isolated from switchgrass and strain R1Gly, while isolated from tobacco, is demonstrated herein to colonize switchgrass. Each strain exhibited highly diverse genomic and phenotypic PGP capabilities. Strain F10Cl and R1Gly demonstrated the highest functional similarity, suggesting that, while endophyte community structure may vary widely based on host species, differences in functional diversity are not a clearly delineated. The results of this study advance our understanding of diazotrophic endophyte diversity, which will allow us to design robust strategies to improve cultivation methods of many economically important commodity crops.

scholarly journals Diversity of Endophytic Bacteria Associated with Different Cultivars of Medicinal Plant Arctium Lappa L. and Their Potential for Host Plant Growth Promoting

2021 ◽  
Author(s):  
Jia-Qi Liu ◽  
Chun-Mei Zhang ◽  
Yuan Gong ◽  
Ming-Jie Xu ◽  
Ke Xing ◽  
...  
Keyword(s):  
Bacterial Community ◽  
Plant Growth ◽  
Endophytic Bacteria ◽  
High Throughput Sequencing ◽  
Promoting Effect ◽  
Pgp Traits ◽  
Arctium Lappa ◽  
Bacterial Phyla ◽  
Plant Growth Promoting ◽  
Growth Promoting

Abstract Purpose Arctium lappa L. is one of the medicinal and food homologous plants in China, which is rich in nutrients and medicinal ingredients. The use of plant growth promoting (PGP) endophytic bacteria is a useful alternative in agricultural production to reduce the use of chemical fertilizers. The aim of this study was to analysis the diversity of endophytic bacteria in different cultivars of A. lappa L. collected from two different geographical locations in China and evaluate PGP traits of the isolates and their potential PGP ability in greenhouse condition. Methods Endophytic bacterial community was investigated by culture-dependent and culture-independent methods. Isolates were screened and investigated for multiple PGP traits, and representative strains were inoculated host seedlings to evaluate the growth promoting effect. Results A total of 348 endophytic bacteria were obtained and they distributed into four phyla, 30 genera and 73 different species. In addition, high throughput sequencing revealed more abundant bacterial community, including 17 bacterial phyla, and 207 different known genera. A high proportion of PGP traits were detected, including production of indole acetic acid, siderophore, ammonia and phosphate solubilization. Four representative strains with multiple PGP traits of the most prevalent genera were further selected for host inoculation and growth promoting evaluation, and they significantly increase seedlings length, root length and fresh weight. Conclusion This study demonstrated that A. lappa L. harbors abundant endophytic bacteria, and plant genotype and geographical origin affect their composition. Moreover, some endophytic bacteria showed good potential for the development of microbial fertilizer in the future.

scholarly journals Characterization of potential plant growth-promoting rhizobacteria isolated from sunflower (Helianthus annuus L.) in Iran

2019 ◽  
Vol 70 (4) ◽  
pp. 268-277
Author(s):  
Nabi Khezrinejad ◽  
Gholam Khodakaramian ◽  
Fatemeh Shahryari
Keyword(s):  
Genetic Diversity ◽  
Plant Growth Promoting Rhizobacteria ◽  
Pgp Traits ◽  
North West ◽  
Rdna Sequences ◽  
16S Rdna Sequences ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Identification And Characterization

Purpose This study aims to characterize plant growth-promoting rhizobacteria (PGPR) in sunflowers growing in different locations at North West of Iran. Materials and methods Sunflower plants were collected from different regions of West Azarbaijan, and rhizospheric bacterial strains were isolated and screened for PGP traits. Identification and characterization of the PGPR were conducted based on 16s rDNA sequences and phenotypic analysis, the strains clustered for genetic diversity by rep-PCR method. Results Among the 80 bacterial isolates, 20 showed PGP traits and were selected for other potentials. All the selected isolates produced indole-3-acetic acid at the rate of 9.2–33.7 mg/ml. In addition, 13, 15, 12, and 16 were positive for phosphate solubilization, siderephore, hydrogen cyanide, and ammonia production, respectively. The results from a subsequent pot experiment indicated that PGPRs distinctly increased sun flower shoot and root length, shoot and root fresh weight, as well as shoot and root dry weight. Based on 16S rDNA sequences and biochemical and physiological characteristics, 20 PGPRs were identified as Pseudomonas fluorescens (five isolates), Pseudomonas aeruginosa (four isolates), Pseudomonas geniculata (one isolate), Bacillus subtilis (four isolates), Bacillus pumilus (two isolates), Stenotrophomonas maltophilia (two isolates), and Brevibacterium frigoritolerans (two isolates). In rep-PCR, PGPR isolates were differentiated into seven clusters (A–G) at 65% similarity level. These results demonstrated the existence of a considerable species richness and genetic diversity among PGPRs isolated from different regions of North West of Iran. Conclusions To the best of our knowledge, this is first report for the identification and characterization of B. frigoritolerans as PGPR in sunflower plants.

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