scholarly journals Oral Administration of T Cell Epitope Peptide Inhibits the Systemic IL-4 Response Elicited by an Egg-White Diet in a TCR Transgenic Mouse Model

2014 ◽  
Vol 33 (1) ◽  
pp. 47-51 ◽  
Author(s):  
Erika HIRAIDE ◽  
Haruyo NAKAJIMA-ADACHI ◽  
Satoshi HACHIMURA
Keyword(s):  
T Cell ◽  
Mouse Model ◽  
Oral Administration ◽  
Transgenic Mouse ◽  
Cell Epitope ◽  
Transgenic Mouse Model ◽  
Egg White ◽  
T Cell Epitope ◽  
Epitope Peptide

A Novel Cellular Pathway of SCL T-Leukemogenesis Revealed by a Conditional Transgenic Mouse Model.

Blood ◽  
2006 ◽  
Vol 108 (11) ◽  
pp. 1835-1835
Author(s):  
Joachim R. Gothert ◽  
Rachael Brake ◽  
C. Glenn Begley ◽  
David J. Izon
Keyword(s):  
T Cell ◽  
Mouse Model ◽  
Transgenic Mouse ◽  
Trichostatin A ◽  
Lymphoblastic Leukemia ◽  
Cell Receptor ◽  
Surface Expression ◽  
Transgenic Mouse Model ◽  
Tamoxifen Treatment ◽  
Double Positive

Abstract The acquired activation of stem cell leukemia (SCL) during T-lymphopoiesis is a common event in T-cell acute lymphoblastic leukemia. Here, we generated a novel tamoxifen-inducible transgenic mouse model (lck-ERT2-SCL) to study the cellular targets of acquired SCL activation during T-cell development. Upon tamoxifen treatment we observed the thymic emergence of abnormal, non-cycling CD8+TCRβlow and immature CD4+CD8+ (double-positive, DP) cells displaying increased viability. Unexpectedly, fetal thymic organ culture analysis of lck-ERT2-SCL thymi revealed the development of DP cells before the emergence of CD8+TCRβlow cells, which implied the derivation of CD8+TCRβlow cells from DPs rather than immature CD8 single-positive (SP) thymocytes. Interestingly, histone deacetylase (HDAC) inhibition with trichostatin A (TSA) had a divergent effect on SCL perturbed thymopoiesis: TSA increased T-cell receptor surface expression within DP and CD8 SP cells however did not alter the CD8 shifted CD4/CD8-ratio. Furthermore, we studied the expression of NOTCH1 in SCL induced TCRβlow thymocytes. Strikingly, we found that SCL induced NOTCH1-upregulation in DP TCRβlow cells. We therefore conclude that SCL promotes the emergence of abnormal CD8+TCRβlow cells by an only partially HDAC dependent mechanism from DP TCRβlow cells. Moreover, SCL induced DP TCRβlow cells are characterized by upregulated NOTCH1, which in turn might promote the effect of acquired NOTCH1 mutations during T-leukemogenesis.

scholarly journals Oral administration of an immunodominant T-cell epitope downregulates Th1/Th2 cytokines and prevents experimental myasthenia gravis

1999 ◽  
Vol 104 (9) ◽  
pp. 1287-1295 ◽  
Author(s):  
Fulvio Baggi ◽  
Francesca Andreetta ◽  
Elisabetta Caspani ◽  
Monica Milani ◽  
Renato Longhi ◽  
...  
Keyword(s):  
T Cell ◽  
Oral Administration ◽  
Myasthenia Gravis ◽  
Cell Epitope ◽  
Th2 Cytokines ◽  
T Cell Epitope

scholarly journals Specific overexpression of IL-7 in the intestinal mucosa: the role in intestinal intraepithelial lymphocyte development

2008 ◽  
Vol 294 (6) ◽  
pp. G1421-G1430 ◽  
Author(s):  
Hua Yang ◽  
Blair Madison ◽  
Deborah L. Gumucio ◽  
Daniel H. Teitelbaum
Keyword(s):  
T Cell ◽  
Mouse Model ◽  
Transgenic Mice ◽  
Transgenic Mouse ◽  
Critical Role ◽  
Transgenic Mouse Model ◽  
Intraepithelial Lymphocytes ◽  
Specific Expression ◽  
And Function

IL-7 plays a crucial role in controlling T cell development and homeostasis. Since IL-7 may be derived from extraintestinal sources, and exogenous IL-7 broadly affects lymphoid populations, the actions of epithelial cell (EC)-derived IL-7 are not fully understood. The effect of intestinal specific expression of IL-7 on intestinal mucosal lymphocytes was investigated by using an IL-7 transgenic mouse model. We generated an intestinal EC-specific overexpressing IL-7 transgenic mouse model (IL-7vill) and compared their phenotype and function to wild-type C57BL/6J mice. EC-derived IL-7 overexpression was found to be exclusively in the small and large intestine. Numbers and subtypes of mucosal lymphocytes, including intraepithelial lymphocytes (IEL) and lamina propria lymphocytes (LPL), significantly changed in IL-7vill mice. From a functional standpoint, IEL proliferation also significantly increased in IL-7vill mice. IEL cytokine expression significantly changed in both T cell receptor (TCR)-αβ+ and TCR-γδ+ IEL subpopulations, including a significant increase in IFN-γ and TNF-α as well as an increase in keratinocyte growth factor expression. EC expression of CD103 (integrin αEβ7), the ligand of E-cadherin, markedly upregulated and may account for a mechanism of the massive expansion of IEL in transgenic mice. Systemic lymphoid populations did not change in transgenic mice. IL-7 overexpression by intestinal EC significantly affected IEL phenotype and function. These results offer insight into the role of IL-7 in IEL development and suggest a critical role of EC-derived expression of IL-7 in the phenotype and function of IEL.

Sign in / Sign up

Export Citation Format

Share Document