scholarly journals Aerobic and Anaerobic Biotransformation of Bile Acids by Escherichia coli (II): No Conversion of ^|^alpha;-Muricholic Acid by 7^|^alpha;-Hydroxysteroid Dehydrogenase of E. coli

2005 ◽  
Vol 24 (2) ◽  
pp. 41-44
Author(s):  
Yoshio Ogura ◽  
Nobuo Yamaga ◽  
Takao Suzuki ◽  
Koji Kimura ◽  
Yasuhiro Kido ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Bile Acids ◽  
Hydroxysteroid Dehydrogenase ◽  
No Conversion ◽  
E Coli ◽  
Anaerobic Biotransformation ◽  
Aerobic And Anaerobic

scholarly journals Aerobic and Anaerobic Biotransformation of Bile Acids by Escherichia coli (I)

2003 ◽  
Vol 22 (4) ◽  
pp. 133-137 ◽  
Author(s):  
Yoshio OGURA ◽  
Nobuo YAMAGA ◽  
Yasuhiro KIDO ◽  
Rie KATAYAMA ◽  
Kazuo YAMADA ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Bile Acids ◽  
Anaerobic Biotransformation ◽  
Aerobic And Anaerobic

A Bacteriological Survey of Raw Ground Beef1

1977 ◽  
Vol 40 (11) ◽  
pp. 790-794 ◽  
Author(s):  
JAMES F. FOSTER ◽  
JAMES L. FOWLER ◽  
WARREN C. LADIGES
Keyword(s):  
Escherichia Coli ◽  
Microbiological Quality ◽  
Retail Store ◽  
Most Probable Number ◽  
Probable Number ◽  
E Coli ◽  
Fecal Streptococci ◽  
Plate Counts ◽  
Aerobic And Anaerobic

The microbiological quality of 150 units of raw ground beef obtained from a local retail store was determined. The range of aerobic plate counts was from 6.9 × 104 to 8.3 × 107/g. By using the most probable number method 96.7% of the 150 units were positive for coliforms, 94.7% for Escherichia coli and 61.3% for Staphylococcus aureus. By the plate methods, 99.3% of the units were positive for fecal streptococci and 56% were positive for Clostridium perfringens. No salmonellae were isolated. Aerobic and anaerobic organisms were isolated and identified. E. coli was the most frequently isolated aerobe followed by organisms in the Klebsiella-Enterobacter group. Among the anaerobic isolates, C. perfringens was the organism most frequently encountered.

scholarly journals Growth of cell wall-defective variants of Escherichia coli: comparison of aerobic and anaerobic induction frequencies

1977 ◽  
Vol 6 (2) ◽  
pp. 166-171
Author(s):  
T W Huber ◽  
A W Brinkley
Keyword(s):  
Escherichia Coli ◽  
Cell Wall ◽  
High Frequency ◽  
Anaerobic Incubation ◽  
Anaerobic Conditions ◽  
Group Iii ◽  
E Coli ◽  
Induction Frequency ◽  
Group I ◽  
Aerobic And Anaerobic

A method for quantitating the conversion of Escherichia coli to colony-forming, cell wall-defective (CWD) bacteria has been developed. The induction frequency, i.e., the percentage of the population recovered as CWD colonies was determined for 20 randomly selected clinical isolates of E. coli under aerobic and anaerobic incubation conditions. Penicillin (1,000 U/ML) was the inducing agent. The 20 strains segregated into three groups. Group I organisms produced CWD colonies with high frequency both aerobically and anaerobically. Grout II organisms showed a much higher induction frequency anaerobically than aerobically. Group III organisms were poor inducers. Thirty percent of the strains were group I, 50% were group II, and 20% were group III organisms. These data indicate that anaerobic conditions enhance the induction and growth of CWD E. coli in the research laboratory and suggest that anaerobic incubation may be important in recovery of medically significant CWD bacteria.

scholarly journals Aerobic and Anaerobic Microbiology of Surgical-Site Infection Following Spinal Fusion

1999 ◽  
Vol 37 (3) ◽  
pp. 841-843 ◽  
Author(s):  
Itzhak Brook ◽  
Edith H. Frazier
Keyword(s):  
Escherichia Coli ◽  
Spinal Fusion ◽  
Bacterial Growth ◽  
Anaerobic Bacteria ◽  
Surgical Site Infections ◽  
Aerobic Bacteria ◽  
Site Infection ◽  
E Coli ◽  
Naval Hospital ◽  
Aerobic And Anaerobic

The aerobic and anaerobic microbiology of surgical-site infections (SSI) following spinal fusion was retrospectively studied. This was done by reviewing the clinical and microbiological records at the Naval Hospital in Bethesda, Md., from 1980 to 1992. Aspirates of pus from 25 infection sites showed bacterial growth. Aerobic bacteria only were recovered from 9 (36%) specimens, anaerobic bacteria only were recovered from 4 (16%), and mixed aerobic and anaerobic bacteria were recovered from 12 (48%). Sixty isolates were recovered: 38 aerobes (1.5 isolates per specimen) and 22 anaerobes (0.9 isolate per specimen). The predominant aerobes were Escherichia coli(n = 8) and Proteus sp. (n = 7). The predominant anaerobes wereBacteroides fragilis group (n = 9) andPeptostreptococcus sp. (n = 6) isolates. An increase in recovery of E. coli and B. fragilis was noted in patients with bowel or bladder incontinence. This study highlights the polymicrobial nature of SSI and the importance of anaerobic bacteria in SSI following spinal fusion.

scholarly journals Effect of Clinically Meaningful Antibiotic Concentrations on Recovery of Escherichia coli and Klebsiella pneumoniae Isolates from Anaerobic Blood Culture Bottles with and without Antibiotic Binding Resins

2019 ◽  
Vol 57 (12) ◽  
Author(s):  
Iris H. Chen ◽  
David P. Nicolau ◽  
Joseph L. Kuti
Keyword(s):  
Escherichia Coli ◽  
Blood Culture ◽  
Klebsiella Pneumoniae ◽  
Blood Cultures ◽  
Content Type ◽  
E Coli ◽  
Detection Systems ◽  
Affect Detection ◽  
Trough Concentrations ◽  
Aerobic And Anaerobic

ABSTRACT Blood cultures are routinely collected in pairs of aerobic and anaerobic bottles. Artificial sterilization of Gram-negative bacteria in aerobic bottles containing clinically meaningful antibiotic concentrations has previously been observed. This study assessed recovery from anaerobic bottles with and without antibiotic binding resins. We studied the recovery of Escherichia coli and Klebsiella pneumoniae when exposed to meropenem, imipenem, cefepime, cefazolin, levofloxacin, and piperacillin-tazobactam in resin-containing BacT/Alert FN Plus and BD Bactec Plus anaerobic/F bottles as well as resin-free BacT/Alert SN and BD Bactec standard anaerobic bottles. Bottles were inoculated with bacteria and whole blood containing peak, midpoint, or trough concentrations and incubated for up to 120 hours in their respective detection systems. In E. coli resin-containing bottles, recovery was observed in 10/24 (42%), 17/24 (71%), and 18/24 (75%) (P = 0.034) of those exposed to peak, midpoint, and trough concentrations, respectively. In K. pneumoniae resin-containing bottles, recovery was observed in 8/16 (50%), 10/16 (63%), and 10/16 (63%) (P = 0.710), respectively. No growth was detected in bottles containing cefepime regardless of concentration, while recovery was observed in the presence of all concentrations of cefazolin and piperacillin-tazobactam. Recovery in bottles with meropenem and imipenem was more frequently observed in BacT/Alert FN Plus bottles compared with Bactec Plus bottles. Resin-free bottles demonstrated significantly lower recovery than bottles containing binding resin. Clinical concentrations of certain antibiotics can adversely affect detection of E. coli and K. pneumoniae in anaerobic blood culture bottles. Obtaining blood cultures immediately before a dose and utilizing resin-containing anaerobic bottles will maximize the likelihood of recovery.

scholarly journals Genome-Wide Analysis of Lipoprotein Expression in Escherichia coli MG1655

2004 ◽  
Vol 186 (10) ◽  
pp. 3254-3258 ◽  
Author(s):  
Stephen J. Brokx ◽  
Michael Ellison ◽  
Troy Locke ◽  
Drell Bottorff ◽  
Laura Frost ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Cell Envelope ◽  
Global Gene Expression ◽  
Growth Conditions ◽  
Rt Pcr ◽  
E Coli ◽  
Genome Wide ◽  
Aerobic And Anaerobic ◽  
Insight Into ◽  
Good Agreement

ABSTRACT To gain insight into the cell envelope of Escherichia coli grown under aerobic and anaerobic conditions, lipoproteins were examined by using functional genomics. The mRNA expression levels of each of these genes under three growth conditions—aerobic, anaerobic, and anaerobic with nitrate—were examined by using both Affymetrix GeneChip E. coli antisense genome arrays and real-time PCR (RT-PCR). Many genes showed significant changes in expression level. The RT-PCR results were in very good agreement with the microarray data. The results of this study represent the first insights into the possible roles of unknown lipoprotein genes and broaden our understanding of the composition of the cell envelope under different environmental conditions. Additionally, these data serve as a test set for the refinement of high-throughput bioinformatic and global gene expression methods.

scholarly journals The effect of various abiotic factors on the survival growth of Escherichia coli and Pseudomonas aeruginosa in bathroom greywater

2012 ◽  
Vol 2 (2) ◽  
pp. 92-101 ◽  
Author(s):  
R. Khalaphallah ◽  
Y. Andres
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Zeta Potential ◽  
Decay Constant ◽  
Abiotic Factors ◽  
Physical Factors ◽  
E Coli ◽  
Low Salinity ◽  
Test Conditions ◽  
Aerobic And Anaerobic

This study was conducted to examine the effect of various abiotic (non-living chemical and physical) factors, which enhance or limit the growth of Escherichia coli and Pseudomonas aeruginosa in bathroom greywater (GW). These factors included: temperature (6 ± 2, 23 ± 2 and 42 ± 2 °C); aeration (aerobic and anaerobic); salinity (1.75 and 3.5%); GW with deionised water (DW) (50% GW:50% DW); and nutrient-rich and -poor medium. The survival period and decay constant (K) were less at 42 ± 2 °C than at 23 ± 2 °C and 6 ± 2 °C. The optimum survival of E. coli and P. aeruginosa was measured under aerobic and anaerobic condition, respectively. P. aeruginosa showed better survival in low salinity concentrations than E. coli. Cell size and zeta potential were also measured for both species under the test conditions.

scholarly journals COMPARISON OF ANTIBACTERIAL ACTIVITY OF HONEY AGAINST AEROBIC AND ANAEROBIC BACTERIA

2021 ◽  
pp. 41-43
Author(s):  
S. DHANARAJ ◽  
S. S. M. UMAMAGESWARI ◽  
M. MALAVIKA ◽  
G. BHUVANESHWARI
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Clostridium Perfringens ◽  
Anaerobic Bacteria ◽  
The Other ◽  
E Coli ◽  
Diffusion Technique ◽  
Aerobic And Anaerobic

Objective: To compare the antibacterial activity of honey against aerobic and anaerobic bacteria. Methods: Honey is extracted from the honey comb by trained persons. Antimicrobial activity of honey is performed by Agar Cup Diffusion technique for 3 bacteria Staphylococcus aureus, E. coli and Clostridium perfringens. Results: By performing the technique with proper guidance, it is observed that the Staphylococcus aureus specimen shows sensitivity to honey whereas the other two specimens Escherichia coli and clostridium perfringens doesn’t show any sensitivity to honey. Conclusion: Due to its vast antibacterial activity of honey, it can be used along with other antibiotics to increase its efficiency.

scholarly journals COMPARISON OF ANTIBACTERIAL ACTIVITY OF GARLIC AGAINST AEROBIC AND ANAEROBIC BACTERIA

2021 ◽  
pp. 44-46
Author(s):  
K. G. DHANUSH ◽  
S. S. M. UMAMAGESWARI ◽  
M. MALAVIKA ◽  
G. BHUVANESHWARI
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Clostridium Perfringens ◽  
Anaerobic Bacteria ◽  
The Other ◽  
E Coli ◽  
Diffusion Technique ◽  
Aerobic And Anaerobic

Objective: To compare the antibacterial activity of garlic against aerobic and anaerobic bacteria. Methods: Antimicrobial activity of garlic is performed by Agar cup diffusion technique for 3 bacteria Staphylococcus aureus, E. coli and clostridium perfringens. Results: By performing the technique with proper guidance, it is observed that the Staphylococcus aureus specimen shows sensitivity to garlic whereas the other two specimens Escherichia coli and clostridium perfringens doesn’t show any sensitivity to garlic. Conclusion: Due to its vast antibacterial activity of garlic, it can be used along with other antibiotics to increase its efficiency.

scholarly journals Metabolic engineering of Escherichia coli for de novo biosynthesis of vitamin B12

2018 ◽  
Author(s):  
Huan Fang ◽  
Dong Li ◽  
Jie Kang ◽  
Pingtao Jiang ◽  
Jibin Sun ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Metabolic Engineering ◽  
Biosynthetic Pathway ◽  
Rhodobacter Capsulatus ◽  
De Novo ◽  
Vitamin B ◽  
E Coli ◽  
De Novo Biosynthesis ◽  
Optimization Of Fermentation ◽  
Aerobic And Anaerobic

ABSTRACTThe only known source of vitamin B12 (adenosylcobalamin) is from bacteria and archaea, and the only unknown step in its biosynthesis is the production of the intermediate adenosylcobinamide phosphate. Here, using genetic and metabolic engineering, we generated an Escherichia coli strain that produces vitamin B12 via an engineered de novo aerobic biosynthetic pathway. Excitingly, the BluE and CobC enzymes from Rhodobacter capsulatus transform L-threonine into (R)-1-Amino-2-propanol O-2-Phosphate, which is then condensed with adenosylcobyric acid to yield adenosylcobinamide phosphate by either CobD from the aeroic R. capsulatus or CbiB from the anerobic Salmonella typhimurium. These findings suggest that the biosynthetic steps from co(II)byrinic acid a,c-diamide to adocobalamin are the same in both the aerobic and anaerobic pathways. Finally, we increased the vitamin B12 yield of a recombinant E. coli strain by more than ∼250-fold to 307.00 µg/g DCW via metabolic engineering and optimization of fermentation conditions. Beyond our scientific insights about the aerobic and anaerobic pathways and our demonstration of E. coli as a microbial biosynthetic platform for vitamin B12 production, our study offers an encouraging example of how the several dozen proteins of a complex biosynthetic pathway can be transferred between organisms to facilitate industrial production.

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