scholarly journals Effect of Body Condition Score of the Dairy Cow on the in vitro Immune Response of Peripheral Blood Mononuclear Cells to Progesterone Stimulation

2009 ◽  
Vol 71 (5) ◽  
pp. 549-553 ◽  
Author(s):  
Hiromichi OHTSUKA ◽  
Yurie MURASE ◽  
Takaaki ANDO ◽  
Masayuki KOHIRUIMAKI ◽  
Machiko MUKAI ◽  
...  
Keyword(s):  
Immune Response ◽  
Body Condition ◽  
Dairy Cow ◽  
Mononuclear Cells ◽  
Body Condition Score ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells ◽  
Condition Score ◽  
In Vitro Immune Response

scholarly journals Bisphenol A suppresses Th1-type immune response in human peripheral blood mononuclear cells in vitro

2015 ◽  
Vol 168 (2) ◽  
pp. 285-292 ◽  
Author(s):  
Johanna M. Gostner ◽  
Emanuel Raggl ◽  
Kathrin Becker ◽  
Florian Überall ◽  
Harald Schennach ◽  
...  
Keyword(s):  
Immune Response ◽  
Bisphenol A ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Human Peripheral Blood ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells

scholarly journals Differential cytokine response of Escherichia coli lipopolysaccharide stimulated peripheral blood mononuclear cells in crossbred cattle, Tharparkar cattle and Murrah buffalo - An in vitro study

2019 ◽  
Vol 17 (1) ◽  
pp. e0501 ◽  
Author(s):  
Sourabh Sulabh ◽  
Manjit Panigrahi ◽  
Satish Kumar ◽  
Rajat Varshney ◽  
Ankita Verma ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Immune Response ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Crossbred Cattle ◽  
Peripheral Blood Mononuclear ◽  
Tharparkar Cattle ◽  
Blood Mononuclear Cells

Mastitis is a complex disease responsible for huge economic losses to the dairy sector. The causal organisms include a wide variety of micro-organisms including several species of bacteria. Escherichia coli has been identified as one of the most common gram-negative bacteria causing clinical mastitis in cattle. The immune system, of different species and/or breeds, tries to combat these pathogens in an inconsistent manner with differential mode and intensity of immune response, eventually producing contradicting outcomes of this disease. Several reports suggest the existence of variability among different animal breeds/species, resulting in a dissimilar outcome of this disease among them. In order to evaluate the variation among different breeds/species, the present study was undertaken to examine the stimulant effect of E. coli lipopolysaccharide (LPS) on peripheral blood mononuclear cells (PBMCs). The PBMCs were harvested from blood samples of crossbred cattle, Tharparkar cattle and Murrah buffaloes. After 6 h of in vitro stimulation, qRT-PCR was employed to measure the relative mRNA expression levels of CCL5, IL-1β, IL-12β, IFN-γ and IL-10 genes in stimulated and unstimulated PBMCs. The selected genes revealed significant differences in the pattern of innate immune response among crossbred cattle, Tharparkar cattle and Murrah buffaloes. The results clearly indicate the presence of variation in the outcome of immune response even when the immunocytes were stimulated with the same dose of the antigen.

scholarly journals Potential Role and Impact of Peripheral Blood Mononuclear Cells in Radiographic Axial Spondyloarthritis-Associated Endothelial Dysfunction

Diagnostics ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1037
Author(s):  
Patricia Ruiz-Limon ◽  
Maria L. Ladehesa-Pineda ◽  
Clementina Lopez-Medina ◽  
Chary Lopez-Pedrera ◽  
Maria C. Abalos-Aguilera ◽  
...  
Keyword(s):  
Endothelial Dysfunction ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Axial Spondyloarthritis ◽  
Endothelial Injury ◽  
In Vitro Studies ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells

Endothelial dysfunction (ED) is well known as a process that can lead to atherosclerosis and is frequently presented in radiographic axial spondyloarthritis (r-axSpA) patients. Here, we investigated cellular and molecular mechanisms underlying r-axSpA-related ED, and analyzed the potential effect of peripheral blood mononuclear cells (PBMCs) in promoting endothelial injury in r-axSpA. A total of 30 r-axSpA patients and 32 healthy donors (HDs) were evaluated. The endothelial function, inflammatory and atherogenic profile, and oxidative stress were quantified. In vitro studies were designed to evaluate the effect of PBMCs from r-axSpA patients on aberrant endothelial activation. Compared to HDs, our study found that, associated with ED and the plasma proatherogenic profile present in r-axSpA, PBMCs from these patients displayed a pro-oxidative, proinflammatory, and proatherogenic phenotype, with most molecular changes noticed in lymphocytes. Correlation studies revealed the relationship between this phenotype and the microvascular function. Additional in vitro studies confirmed that PBMCs from r-axSpA patients promoted endothelial injury. Altogether, this study suggests the relevance of r-axSpA itself as a strong and independent cardiovascular risk factor, contributing to a dysfunctional endothelium and atherogenic status by aberrant activation of PBMCs. Lymphocytes could be the main contributors in the development of ED and subsequent atherosclerosis in this pathology.

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