scholarly journals Relationship Between Growth Behavior in Vero Cells and the Molecular Characteristics of Recent Isolated Classified in the Asia 1 and 2 Groups of Canine Distemper Virus

2009 ◽  
Vol 71 (4) ◽  
pp. 457-461 ◽  
Author(s):  
Nguyen Thi LAN ◽  
Ryoji YAMAGUCHI ◽  
Takuya HIRAI ◽  
Kazushige KAI ◽  
Kazuhiro MORISHITA
Keyword(s):  
Canine Distemper Virus ◽  
Vero Cells ◽  
Growth Behavior ◽  
Molecular Characteristics ◽  
Canine Distemper

Canine Distemper Virus Induces Apoptosis Through Caspase-3 and -8 Activation in Vero Cells

2006 ◽  
Vol 53 (6) ◽  
pp. 273-277 ◽  
Author(s):  
M. Kajita ◽  
H. Katayama ◽  
T. Murata ◽  
C. Kai ◽  
M. Hori ◽  
...  
Keyword(s):  
Canine Distemper Virus ◽  
Caspase 3 ◽  
Vero Cells ◽  
Canine Distemper

Inhibition of canine distemper virus replication by blocking pyrimidine nucleotide synthesis with A77 1726, the active metabolite of the anti-inflammatory drug leflunomide

2021 ◽  
Author(s):  
Yao Li ◽  
Li Yi ◽  
Sipeng Cheng ◽  
Yongshan Wang ◽  
Jiongjiong Wang ◽  
...  
Keyword(s):  
Antiviral Activity ◽  
Active Metabolite ◽  
Canine Distemper Virus ◽  
De Novo ◽  
Specific Inhibitor ◽  
Vero Cells ◽  
Canine Distemper ◽  
Nucleotide Synthesis ◽  
Pyrimidine Nucleotide ◽  
Rate Limiting

Canine distemper virus (CDV) is the aetiological agent that causes canine distemper (CD). Currently, no antiviral drugs have been approved for CD treatment. A77 1726 is the active metabolite of the anti-rheumatoid arthritis (RA) drug leflunomide. It inhibits the activity of Janus kinases (JAKs) and dihydroorotate dehydrogenase (DHO-DHase), a rate-limiting enzyme in de novo pyrimidine nucleotide synthesis. A77 1726 also inhibits the activity of p70 S6 kinase (S6K1), a serine/threonine kinase that phosphorylates and activates carbamoyl-phosphate synthetase (CAD), a second rate-limiting enzyme in the de novo pathway of pyrimidine nucleotide synthesis. Our present study focuses on the ability of A77 1726 to inhibit CDV replication and its underlying mechanisms. Here we report that A77 1726 decreased the levels of the N and M proteins of CDV and lowered the virus titres in the conditioned media of CDV-infected Vero cells. CDV replication was not inhibited by Ruxolitinib (Rux), a JAK-specific inhibitor, but by brequinar sodium (BQR), a DHO-DHase-specific inhibitor, and PF-4708671, an S6K1-specific inhibitor. Addition of exogenous uridine, which restores intracellular pyrimidine nucleotide levels, blocked the antiviral activity of A77 1726, BQR and PF-4708671. A77 1726 and PF-4708671 inhibited the activity of S6K1 in CDV-infected Vero cells, as evidenced by the decreased levels of CAD and S6 phosphorylation. S6K1 knockdown suppressed CDV replication and enhanced the antiviral activity of A77 1726. These observations collectively suggest that the antiviral activity of A77 1726 against CDV is mediated by targeting pyrimidine nucleotide synthesis via inhibiting DHO-DHase activity and S6K1-mediated CAD activation.

scholarly journals Canine distemper virus detection in asymptomatic and non vaccinated dogs

2010 ◽  
Vol 30 (2) ◽  
pp. 139-144 ◽  
Author(s):  
Helen L. Del Puerto ◽  
Anilton C. Vasconcelos ◽  
Luciana Moro ◽  
Fabiana Alves ◽  
Gissandra F. Braz ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Canine Distemper Virus ◽  
Early Stage ◽  
Virus Detection ◽  
Vero Cells ◽  
Canine Distemper ◽  
Coding Region ◽  
Protein Coding ◽  
Dissociation Curves

A quantitative real time polymerase chain reaction (PCR) revealed canine distemper virus presence in peripheral blood samples from asymptomatic and non vaccinated dogs. Samples from eleven domestic dogs with no signs of canine distemper and not vaccinated at the month of collection were used. Canine distemper virus vaccine samples in VERO cells were used as positive controls. RNA was isolated with Trizol®, and treated with a TURBO DNA-free kit. Primers were designed for canine distemper virus nucleocapsid protein coding region fragment amplification (84 bp). Canine b-actin (93 bp) was utilized as the endogenous control for normalization. Quantitative results of real time PCR generated by ABI Prism 7000 SDS Software showed that 54.5% of dogs with asymptomatic canine distemper were positive for canine distemper virus. Dissociation curves confirmed the specificity of the real time PCR fragments. This technique could detect even a few copies of viral RNA and identificate subclinically infected dogs providing accurate diagnosis of this disease at an early stage.

scholarly journals Requirement of Methionine for the Replication of Canine Distemper Virus in Vero Cells

1985 ◽  
Vol 66 (1) ◽  
pp. 149-157 ◽  
Author(s):  
N. Hirayama ◽  
M. Senda ◽  
K. Kurata ◽  
Y. Yoshikawa ◽  
K. Yamanouchi
Keyword(s):  
Canine Distemper Virus ◽  
Vero Cells ◽  
Canine Distemper

Role of Actin Microfilaments in Canine Distemper Virus Replication in Vero Cells

2004 ◽  
Vol 66 (4) ◽  
pp. 409-415 ◽  
Author(s):  
Hideo KATAYAMA ◽  
Masatoshi HORI ◽  
Koichi SATO ◽  
Masahiro KAJITA ◽  
Hiroshi OZAKI ◽  
...  
Keyword(s):  
Virus Replication ◽  
Canine Distemper Virus ◽  
Vero Cells ◽  
Actin Microfilaments ◽  
Canine Distemper
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