scholarly journals Detection of Babesia gibsoni by Reaction of Phage Display Single Chain Antibodies with P50 Proteins

2009 ◽  
Vol 71 (8) ◽  
pp. 1133-1136 ◽  
Author(s):  
Yoshino HIROSE ◽  
Saeka MATSUHIRA ◽  
Tatiana Alexandrovna BATANOVA ◽  
Youn-Kyoung GOO ◽  
Mohamad A. TERKAWI ◽  
...  
Keyword(s):  
Phage Display ◽  
Single Chain ◽  
Babesia Gibsoni ◽  
Single Chain Antibodies

scholarly journals Identification of single chain antibodies to breast cancer stem cells using phage display

2009 ◽  
pp. NA-NA ◽  
Author(s):  
Deniz Gur ◽  
Suling Liu ◽  
Anurag Shukla ◽  
Stephanie C Pero ◽  
Max S Wicha ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Stem Cells ◽  
Cancer Stem Cells ◽  
Phage Display ◽  
Breast Cancer Stem Cells ◽  
Single Chain ◽  
Single Chain Antibodies

Preparation of Functional Single-Chain Antibodies against Bioactive Gibberellins by Utilizing Randomly Mutagenized Phage-Display Libraries

2005 ◽  
Vol 69 (3) ◽  
pp. 610-619 ◽  
Author(s):  
Yoshihito SUZUKI ◽  
Shinsaku ITO ◽  
Kaori OTSUKA ◽  
Eriko IWASAWA ◽  
Masatoshi NAKAJIMA ◽  
...  
Keyword(s):  
Phage Display ◽  
Single Chain ◽  
Single Chain Antibodies ◽  
Phage Display Libraries

scholarly journals Human single-chain antibodies that neutralize Pseudomonas aeruginosa-exotoxin A-mediated cellular apoptosis

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Sirijan Santajit ◽  
Watee Seesuay ◽  
Kodchakorn Mahasongkram ◽  
Nitat Sookrung ◽  
Sumate Ampawong ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Phage Display ◽  
Virulence Factors ◽  
Catalytic Domain ◽  
Exotoxin A ◽  
Single Chain ◽  
New Paradigm ◽  
Phage Display Technology ◽  
Single Chain Antibodies ◽  
Therapeutic Regime

Abstract Targeting bacterial virulence factors directly provides a new paradigm for the intervention and treatment of bacterial diseases. Pseudomonas aeruginosa produces a myriad of virulence factors to cause fatal diseases in humans. In this study, human single-chain antibodies (HuscFvs) that bound to P. aeruginosa exotoxin A (ETA) were generated by phage display technology using recombinant ETA, ETA-subdomains and the synthetic peptide of the ETA-catalytic site as baits for selecting ETA-bound-phages from the human-scFv phage display library. ETA-bound HuscFvs derived from three phage-transfected E. coli clones neutralized the ETA-induced mammalian cell apoptosis. Computerized simulation demonstrated that these HuscFvs used several residues in their complementarity-determining regions (CDRs) to form contact interfaces with the critical residues in ETA-catalytic domain essential for ADP-ribosylation of eukaryotic elongation factor 2, which should consequently rescue ETA-exposed-cells from apoptosis. The HuscFv-treated ETA-exposed cells also showed decremented apoptosis-related genes, i.e., cas3 and p53. The effective HuscFvs have high potential for future evaluation in animal models and clinical trials as a safe, novel remedy for the amelioration of exotoxin A-mediated pathogenesis. HuscFvs may be used either singly or in combination with the HuscFv cognates that target other P. aeruginosa virulence factors as an alternative therapeutic regime for difficult-to-treat infections.

Isolation and Characterization of Neutralizing Single-Chain Antibodies againstXenopusMitogen-Activated Protein Kinase Kinase from Phage Display Libraries†

Biochemistry ◽  
1996 ◽  
Vol 35 (40) ◽  
pp. 13212-13221 ◽  
Author(s):  
Hidetaka Kosako ◽  
Yoshiko Akamatsu ◽  
Naoya Tsurushita ◽  
Kyung-Kwon Lee ◽  
Yukiko Gotoh ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Phage Display ◽  
Single Chain ◽  
Single Chain Antibodies ◽  
Isolation And Characterization ◽  
Phage Display Libraries ◽  
Protein Kinase Kinase

scholarly journals Generation of Novel Single-Chain Antibodies by Phage-Display Technology to Direct Imaging Agents Highly Selective to Pancreatic  - or  -Cells In Vivo

Diabetes ◽  
2009 ◽  
Vol 58 (10) ◽  
pp. 2324-2334 ◽  
Author(s):  
S. Ueberberg ◽  
J. J. Meier ◽  
C. Waengler ◽  
W. Schechinger ◽  
J. W. Dietrich ◽  
...  
Keyword(s):  
Phage Display ◽  
Imaging Agents ◽  
Single Chain ◽  
Direct Imaging ◽  
Phage Display Technology ◽  
Single Chain Antibodies ◽  
Display Technology
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