scholarly journals Comparative Study of In situ Hybridization and Immunohistochemistry for the Detection of Porcine Circovirus 2 in Formalin-Fixed, Paraffin-Embedded Tissues

2009 ◽  
Vol 71 (7) ◽  
pp. 1001-1004 ◽  
Author(s):  
Duyeol KIM ◽  
Yooncheol HA ◽  
Yong-Hoon LEE ◽  
Sungwon CHAE ◽  
Kichan LEE ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Comparative Study ◽  
Porcine Circovirus ◽  
Formalin Fixed Paraffin ◽  
Porcine Circovirus 2 ◽  
Formalin Fixed Paraffin Embedded ◽  
Formalin Fixed

scholarly journals Optimal Probe Size and Fixation Time for the Detection of Porcine Circovirus–2 DNA by in Situ Hybridization in Formalin-Fixed, Paraffin-Embedded Tissue

2009 ◽  
Vol 21 (5) ◽  
pp. 649-654 ◽  
Author(s):  
Yooncheol Ha ◽  
Chanhee Chae
Keyword(s):  
In Situ Hybridization ◽  
Lymph Nodes ◽  
Porcine Circovirus ◽  
Fixation Time ◽  
Formalin Fixed Paraffin ◽  
Probe Size ◽  
Formalin Fixed Paraffin Embedded ◽  
The Difference ◽  
Formalin Fixed

Probe size and fixation time for detecting Porcine circovirus–2 (PCV–2) by in situ hybridization in formalin-fixed, paraffin-embedded lymph nodes from experimentally infected pigs were optimized. In situ hybridization using a 169–base pair (bp) probe detected significantly fewer PCV–2–positive cells than when using 8 other larger probes ( P < 0.05). The difference in hybridization intensity between smaller probes (169 and 225 bp) and larger probes (416, 473, 571, 631, 693, and 753 bp) was statistically significant ( P < 0.05). The PCV–2–positive cells were consistently detected in lymph nodes fixed up to 3 days; thereafter, the number of positive cells declined. The PCV–2–positive cells were detected in lymph nodes fixed for up to 730 days. The difference in hybridization intensity between samples fixed for a short term (1 or 3 days) and a longer term (4–730 days) was statistically significant ( P < 0.05). The data demonstrates that the optimal probe size and fixation time for detecting PCV–2 in formalin-fixed, paraffin-embedded lymph nodes is 473 bp and 1–3 days, respectively.

Canine Hepatitis Associated with Intrahepatic Bacteria in Three Dogs

2018 ◽  
Vol 54 (1) ◽  
pp. 65-70 ◽  
Author(s):  
Joon Im ◽  
Derek P. Burney ◽  
Sean P. McDonough ◽  
Brigid Nicholson ◽  
Adam Eatroff ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Case Report ◽  
Fluorescence In Situ Hybridization ◽  
Antimicrobial Therapy ◽  
Granulomatous Inflammation ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Hematoxylin And Eosin ◽  
Formalin Fixed

ABSTRACT This case report describes the detection of intrahepatic bacteria in formalin-fixed paraffin-embedded histopathological sections from three dogs with neutrophilic, pyogranulomatous, or lymphoplasmacytic hepatitis and cholangiohepatitis. In each of these cases, eubacterial fluorescence in situ hybridization enabled colocalization of intrahepatic bacteria with neutrophilic and granulomatous inflammation in samples that were negative for bacteria when evaluated by routine hematoxylin and eosin histopathology augmented with histochemical stains. Positive responses to antimicrobial therapy were observed in of 2 out of 2 patients that were treated with antimicrobials. These findings suggest that eubacterial fluorescence in situ hybridization analysis of formalin-fixed paraffin-embedded histopathological sections is more sensitive than conventional histochemical stains for the diagnosis of bacteria-associated canine hepatitis.

Sign in / Sign up

Export Citation Format

Share Document