scholarly journals Isolation of Lawsonia intracellularis in Korea and Reproduction of Proliferative Enteropathy in Pigs and Hamsters

2006 ◽  
Vol 68 (5) ◽  
pp. 499-501 ◽  
Author(s):  
Jung-Yong YEH ◽  
Tae-Jong KIM ◽  
Seung-Yong PARK ◽  
Chang-Seon SONG ◽  
Yong-Dhuk YOON ◽  
...  
Keyword(s):  
Lawsonia Intracellularis ◽  
Proliferative Enteropathy

scholarly journals Draft Genome Sequence of Lawsonia intracellularis Strain E40504, Isolated from a Horse Diagnosed with Equine Proliferative Enteropathy

2017 ◽  
Vol 5 (19) ◽  
Author(s):  
Nandita S. Mirajkar ◽  
Molly R. Kelley ◽  
Connie J. Gebhart
Keyword(s):  
Genome Sequence ◽  
Genomic Island ◽  
Draft Genome ◽  
Draft Genome Sequence ◽  
Lawsonia Intracellularis ◽  
Protein Coding ◽  
Content Type ◽  
Obligate Intracellular ◽  
Proliferative Enteropathy ◽  
Rna Genes

ABSTRACT Reported herein is the draft genome sequence of equine-origin Lawsonia intracellularis strain E40504, an obligate intracellular bacterium and the etiological agent of equine proliferative enteropathy. The 1.69-Mb draft genome sequence includes 1,380 protein-coding genes and 49 RNA genes, and it lacks a genomic island reported in swine-origin L. intracellularis strain PHE/MN1-00.

scholarly journals Histopathological changes in pigs infected with ileitis

2020 ◽  
Vol 10 (4) ◽  
pp. 72-77
Author(s):  
O.M. Yermolenko ◽  
O.Y. Ayshpur ◽  
I.Yu. Mushtuk
Keyword(s):  
Histological Examination ◽  
Severe Disease ◽  
Gastrointestinal Diseases ◽  
Pathological Changes ◽  
Histopathological Changes ◽  
Lawsonia Intracellularis ◽  
Early Diagnostics ◽  
Chronic Effect ◽  
Anatomical Changes ◽  
Proliferative Enteropathy

Nowadays, the ileitis (proliferative enteropathy of pigs) is one of the most common gastrointestinal diseases. The intracellular bacterium Lawsonia intracelluaris causes several forms of the disease. Histological examinations are the important stages in diagnosing and detecting profound pathological changes in sick pig organs, decreasing their productivity and causing the deaths. Swine proliferative enteropathy (SPE) has reported for the Ukraine since 2008. For SPE the early diagnostics with different test systems is relevant. We evaluated the sick, forcibly killed and dead piglets of different ages in Ukrainian pig farms during 2018-2019 in animal fattening groups. Materials were taken from piglets aged 120-150 days in farms where the ileitis was diagnosed. To assess the complex of pathological and anatomical changes detected during autopsies of piglets, V.P. Shishkov’s methods were used. Thus, we revealed a characteristic morphology of proliferative lesions. We believed that the histological examination can serve as an important diagnostic method and an alternative to direct isolation of Lawsonia intracellularis, which is very difficult to cultivate. We determined that the histopathological changes in pig organs indicated a chronic effect caused by animal pathogen. We revealed that Lawsonia intracellularis caused the profound pathological changes in pig digestive tracts, which affected the functioning of all organs and tissues of animals and lead to severe disease and possible animal death. We suggested that the histological examination is an important step in diagnosing and detecting profound pathological changes in the organs of sick pigs, which caused the decrease in their productivity and deaths.

scholarly journals Tylosin injectable for the treatment of porcine proliferative enteropathy in experimentally inoculated pigs

2019 ◽  
Vol 39 (3) ◽  
pp. 168-174
Author(s):  
Luisa V.A. Otoni ◽  
Michelle P. Gabardo ◽  
Núbia R. Macêdo ◽  
Mariane M. Wagatsuma ◽  
Marina M. Pereira ◽  
...  
Keyword(s):  
Body Condition Score ◽  
Initial Period ◽  
Etiologic Agent ◽  
Economic Losses ◽  
Control Group ◽  
Lawsonia Intracellularis ◽  
Medication Group ◽  
Proliferative Enteropathy ◽  
Condition Score ◽  
And Control

ABSTRACT: Porcine proliferative enteropathy (PPE) is one of the most common enteric diseases in growing and finishing pigs. PPE is characterized by reduced growth performance, accompanied or not by diarrhea. PPE is highly prevalent in several countries of the Americas, Europe and Asia, causing high economic losses in swine herds. The most common form of PPE control in pigs is antibiotic therapy. The objective of this study was to evaluate a new product based on tylosin injectable (Eurofarma Laboratórios S.A.) to control PPE in experimentally inoculated animals. Sixty 5-week-old pigs with mean weight of 9.5kg were divided into two experimental groups of 30 animals: medication and control. All pigs were challenged with Lawsonia intracellularis, the etiologic agent of PPE, on day zero. Fecal score, body condition score, and behavior were daily evaluated. Pigs were weighted on days -2, 13 and 21 of the experiment. Pigs in the Medication Group received tylosin injectable 13 days after inoculation, in three doses with a 12-hour interval between them. Pigs in the Control Group received injectable saline solution following the same protocol. In the Control Group, 23pigs presented with diarrhea before day 13. After day 13, the number of diarrheic animals in this group was reduced to 17. In the Medication Group, 26 pigs presented with diarrhea in the initial period, and in the period after medication, only 11 animals had diarrhea. The score of gross intestinal PPE lesions in the Medication Group was lower than that in the Control Group (p=0.031). The Medication Group also showed lower score for Lawsonia intracellularis antigen-labeling by immunohistochemistry compared with that of the Control Group (p=0.032), showing lower level of infection. These results demonstrate that tylosin injectable (Eurofarma Laboratórios S.A.), administrated in three doses (1mL/20kg) every 12 hours, was effective for the control of PPE in experimentally inoculated pigs.

Detection of Lawsonia intracellularis in the tonsils of pigs with proliferative enteropathy

2000 ◽  
Vol 68 (1) ◽  
pp. 23-26 ◽  
Author(s):  
T.K. JENSEN ◽  
K. MØLLER ◽  
R. LINDECRONA ◽  
S.E. JORSAL
Keyword(s):  
Lawsonia Intracellularis ◽  
Proliferative Enteropathy

scholarly journals Specific Detection of Lawsonia intracellularis in Porcine Proliferative Enteropathy Inferred from Fluorescent rRNA In Situ Hybridization

1998 ◽  
Vol 35 (2) ◽  
pp. 153-156 ◽  
Author(s):  
M. Boye ◽  
T. K. Jensen ◽  
K. Møller ◽  
T. D. Leser ◽  
S. E. Jorsal
Keyword(s):  
In Situ Hybridization ◽  
Fluorescent In Situ Hybridization ◽  
Lawsonia Intracellularis ◽  
Specific Detection ◽  
Fixed Tissue ◽  
Tissue Samples ◽  
Formalin Fixed Tissue ◽  
Proliferative Enteropathy ◽  
Formalin Fixed

Fluorescent in situ hybridization targeting 16S ribosomal RNA was used for specific detection of the obligate intracellular bacterium Lawsonia intracellularis in enterocytes from pigs affected by proliferative enteropathy. A specific oligonucleotide probe was designed and the specificity of the probe was determined by simultaneous comparison with indirect immunofluorescence assay for detection of L. intracellularis in formalin-fixed tissue samples from 15 pigs affected by porcine proliferative enteropathy. We used 10 tissue samples from pigs without proliferative mucosal changes as negative controls. The results showed that the oligonucleotide probe is specific for L. intracellularis and that fluorescent in situ hybridization targeting ribosomal RNA is a suitable and fast method for specific detection and histological recognition of L. intracellularis in formalin-fixed tissue.

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