scholarly journals Molecular Typing of VapA-Positive Rhodococcus equi Isolates from Jeju Native Horses, Korea

2006 ◽  
Vol 68 (3) ◽  
pp. 249-253 ◽  
Author(s):  
Won-Geun SON ◽  
Du-Sik LEE ◽  
Noriko YAMATODA ◽  
Fumiko HATORI ◽  
Nanako SHIMIZU ◽  
...  
Keyword(s):  
Molecular Typing ◽  
Rhodococcus Equi

scholarly journals Molecular Epidemiology of Rhodococcus equi Based on traA, vapA, and vapB Virulence Plasmid Markers

2007 ◽  
Vol 196 (5) ◽  
pp. 763-769 ◽  
Author(s):  
Alain A Ocampo-Sosa ◽  
Deborah A Lewis ◽  
Jesús Navas ◽  
Frances Quigley ◽  
Raquel Callejo ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Molecular Typing ◽  
Rhodococcus Equi ◽  
Virulence Plasmid ◽  
Chain Reaction ◽  
Gene Markers ◽  
Typing System ◽  
New Type ◽  
Polymerase Chain ◽  
Related Plasmid

AbstractMolecular typing of the actinomycete Rhodococcus equi is insufficiently developed, and little is known about the epidemiology and transmission of this multihost pathogen. We report a simple, reliable polymerase chain reaction typing system for R. equi based on 3 plasmid gene markers: traA from the conserved conjugal transfer machinery and vapA and vapB, found in 2 different plasmid subpopulations. This “TRAVAP” typing scheme classifies R. equi into 4 categories: traA+/vapA+B−, traA+/vapA−B+, traA+/vapAB−, and traA−/vapAB− (plasmidless). A TRAVAP survey of 215 R. equi strains confirmed the strong link between vapA (traA+/vapA+B− plasmids) and horse isolates and revealed other host-related plasmid associations: between traA+/vapA−B+ and pigs and between traA+/vapAB−—a new type of R. equi plasmid—and cattle. Plasmidless strains were more frequent among isolates from nonpathological specimens. All plasmid categories were common in human isolates, which possibly reflects the predominantly opportunistic nature of R. equi infection in this host and a zoonotic origin.

Molecular Typing of Toxoplasma Gondii Isolated from Infertile Men and Its Effect on the Reproduction

2018 ◽  
Vol 14 (1) ◽  
pp. 141-152
Author(s):  
Nazar Mohammed ◽  
◽  
Farhan Risan ◽  
Salwa Muhsin
Keyword(s):  
Toxoplasma Gondii ◽  
Molecular Typing ◽  
Infertile Men

An Outbreak of ESBL-producing Klebsiella pneumoniae in an Iranian Referral Hospital: Epidemiology and Molecular Typing

2019 ◽  
Vol 19 (1) ◽  
pp. 46-54 ◽  
Author(s):  
Shima Mahmoudi ◽  
Babak Pourakbari ◽  
Aliakbar Rahbarimanesh ◽  
Mohammad Reza Abdosalehi ◽  
Keyghobad Ghadiri ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Nosocomial Infections ◽  
Molecular Typing ◽  
Genetic Relatedness ◽  
Referral Hospital ◽  
Limited Information ◽  
Hospital Epidemiology ◽  
Rapd Pcr ◽  
Published Report ◽  
Random Amplification

Introduction: Klebsiella pneumoniae is a common cause of nosocomial infections; however, there is limited information in Iran regarding nosocomial outbreaks due to extended-spectrum β–lactamase (ESBL) producing K pneumoniae strains, particularly using molecular methods. The present study focused on the molecular mechanism of ESBL resistance and genetic relatedness in K. pneumoniae isolates causing nosocomial infections in an Iranian referral hospital. Material and Methods: This study evaluated the antimicrobial resistance and molecular epidemiology of K. pneumoniae causing nosocomial infections in children between October 2013 and March 2014. The ESBL detection was carried out for all the isolates by the CLSI method and PCR was carried out for the detection of the blaSHV, blaTEM, and blaCTX-M genes among ESBL-producing K. pneumonia. Molecular typing of the K. pneumoniae was performed using random amplification of polymorphic DNA-polymerase chain reaction (RAPD-PCR). Results: A total of 30 isolates of K. pneumoniae were used for epidemiological analysis. High rates of resistance to cefotaxime (n=29, 97%), cefazolin (n=29, 97%), cefepime (n=25, 83%) and gentamicin (n=23, 77%) were observed. A total of 29 strains (97%) produced ESBLs. The frequency of blaSHV, blaCTX-M and blaTEM genes among these isolates was 83% (n=25), 70% (n=21) and 57% (n=17), respectively. Surprisingly 11 isolated (37%) carried blaSHV, blaCTX-M and blaTEM genes simultaneously. Moreover, the concurrent presence of “blaSHV and blaCTX-M” and “blaSHV and blaTEM” was seen in 8 (27%) and 4 (13%) isolates, respectively. RAPDPCR analyses revealed that K. pneumoniae isolates belonged to 2 RAPD-PCR types among which one cluster counted for 28 isolates. Conclusion: To our knowledge, this is the first published report of a nosocomial outbreak of ESBL-producing K. pneumoniae in children in Iran. Although the epidemiology of nosocomial infections with ESBL-producing organisms has not yet been explored in depth in Iran, our findings suggest that ESBL-producing organisms are already an established public health threat in our country.

Molecular typing of multi-drug resistant Candida albicans isolated from the Segamat community, Malaysia

2021 ◽  
Author(s):  
Marie Andrea Laetitia Huët ◽  
Nazmul Hasan Muzahid ◽  
Chuen Zhang Lee ◽  
Calvin Bok Sun Goh ◽  
Jacky Dwiyanto ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Molecular Typing ◽  
Drug Resistant

Plasma lipidome of healthy and Rhodococcus equi ‐infected foals over time

2021 ◽  
Author(s):  
Jorge L. Sanclemente ◽  
Sol Maiam Rivera‐Velez ◽  
Nairanjana Dasgupta ◽  
David W. Horohov ◽  
Paul L. Wood ◽  
...  
Keyword(s):  
Rhodococcus Equi ◽  
Over Time
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