scholarly journals Effects of Various Selective Phosphodiesterase Inhibitors on Carbachol-induced Contraction and Cyclic Nucleotide Contents in Guinea Pig Taenia Coli

2004 ◽  
Vol 66 (9) ◽  
pp. 1047-1052 ◽  
Author(s):  
Takeharu KANEDA ◽  
Kazumasa SHIMIZU ◽  
Norimoto URAKAWA ◽  
Shinjiro NAKAJYO
Keyword(s):  
Guinea Pig ◽  
Cyclic Nucleotide ◽  
Phosphodiesterase Inhibitors ◽  
Taenia Coli

scholarly journals Effects of Various Selective Phosphodiesterase Inhibitors on Carbachol-Induced Contraction and Cyclic Nucleotide Contents in the Guinea Pig Gall Bladder

2005 ◽  
Vol 67 (7) ◽  
pp. 659-665 ◽  
Author(s):  
Takeharu KANEDA ◽  
Akitoshi WATANABE ◽  
Kazumasa SHIMIZU ◽  
Norimoto URAKAWA ◽  
Shinjiro NAKAJYO
Keyword(s):  
Gall Bladder ◽  
Guinea Pig ◽  
Cyclic Nucleotide ◽  
Phosphodiesterase Inhibitors

scholarly journals The identification of a new cyclic nucleotide phosphodiesterase activity in human and guinea-pig cardiac ventricle. Implications for the mechanism of action of selective phosphodiesterase inhibitors

1987 ◽  
Vol 241 (2) ◽  
pp. 535-541 ◽  
Author(s):  
M L Reeves ◽  
B K Leigh ◽  
P J England
Keyword(s):  
Cyclic Amp ◽  
Guinea Pig ◽  
Cardiac Muscle ◽  
Cyclic Gmp ◽  
Cyclic Nucleotide ◽  
Gel Filtration ◽  
Phosphodiesterase Inhibitors ◽  
Cyclic Nucleotide Phosphodiesterase ◽  
Kinetic Properties ◽  
Cardiac Ventricle

Four cyclic nucleotide phosphodiesterase (PDE) activities were separated from low-speed supernatants of homogenates of human cardiac ventricle by DEAE-Sepharose chromatography, and designated PDE I-PDE IV in order of elution with an increasing salt gradient. PDE I was a Ca2+/calmodulin-stimulated activity, and PDE II was an activity with a high Km for cyclic AMP which was stimulated by low concentrations of cyclic GMP. Human ventricle PDE III had Km values of 0.14 microM (cyclic AMP) and 4 microM (cyclic GMP), and showed simple Michaelis-Menten kinetics with both substrates. PDE IV is a previously unrecognized activity in cardiac muscle, the human enzyme having Km values of 2 microM (cyclic AMP) and 50 microM (cyclic GMP). PDE III and PDE IV were not activated by cyclic nucleotides or calmodulin. Four PDE activities were also isolated from guinea-pig ventricle, and had very similar kinetic properties. By gel filtration, the Mr of PDE III was 60,000, and that of PDE IV 45,000. The drug SK&F 94120 selectively and competitively inhibited PDE III with a Ki value of 0.8 microM (human), showing simple hyperbolic inhibition kinetics. Rolipram (Schering ZK 62711) and Ro 20-1724 (Roche), which have previously been reported to inhibit PDE III-like activities strongly, were shown to be weak inhibitors of human and guinea-pig PDE III enzymes (Ki values greater than 25 microM), but potent inhibitors of PDE IV [Ki values 2.4 microM (Rolipram) and 3.1 microM (Ro 20-1724) with human PDE IV]. The inhibition in all cases demonstrated simple hyperbolic competition. These observations suggest that the previously reported complex inhibition of PDE III-type activities from cardiac muscle was caused by incomplete separation of the PDE III from other enzymes, particularly PDE IV.

scholarly journals Inhibitory effect of beauvericin on a high K+-induced tonic contraction in guinea-pig taenia coli.

1987 ◽  
Vol 45 (3) ◽  
pp. 317-325 ◽  
Author(s):  
Shinjiro NAKAJYO ◽  
Kiyomi MATSUOKA ◽  
Tomohiro KITAYAMA ◽  
Yutaka YAMAMURA ◽  
Kazumasa SHIMIZU ◽  
...  
Keyword(s):  
Guinea Pig ◽  
Inhibitory Effect ◽  
Tonic Contraction ◽  
Taenia Coli ◽  
High K

scholarly journals EFFECT OF HISTAMINE ON OXYGEN CONSUMPTION IN GUINEA PIG TAENIA COLI

1971 ◽  
Vol 21 (5) ◽  
pp. 605-611
Author(s):  
Yukio SAITO ◽  
Yutaka SAKAI ◽  
Miyoshi IKEDA ◽  
Norimoto URAKAWA
Keyword(s):  
Oxygen Consumption ◽  
Guinea Pig ◽  
Taenia Coli

scholarly journals EFFECT OF HISTAMINE ON CALCIUM MOVEMENT IN GUINEA PIG TAENIA COLI

1971 ◽  
Vol 21 (5) ◽  
pp. 597-603 ◽  
Author(s):  
Tetsuyuki NASU ◽  
Hideaki KARAKI ◽  
Miyoshi IKEDA ◽  
Norimoto URAKAWA
Keyword(s):  
Guinea Pig ◽  
Taenia Coli
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