scholarly journals Effect of Vascular Endothelial Growth Factor on Maturation, Fertilization and Developmental Competence of Bovine Oocytes

2002 ◽  
Vol 64 (9) ◽  
pp. 803-806 ◽  
Author(s):  
Hailing LUO ◽  
Koji KIMURA ◽  
Mari AOKI ◽  
Makoto HIRAKO
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Developmental Competence ◽  
Vascular Endothelial ◽  
Endothelial Growth Factor ◽  
Bovine Oocytes

scholarly journals Presence of vascular endothelial growth factor during the first half of IVM improves the meiotic and developmental competence of porcine oocytes from small follicles

2017 ◽  
Vol 29 (10) ◽  
pp. 1902 ◽  
Author(s):  
Tra M. T. Bui ◽  
Khánh X. Nguyễn ◽  
Asako Karata ◽  
Pilar Ferré ◽  
Minh T. Trần ◽  
...  
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Formation Rate ◽  
Developmental Competence ◽  
Vascular Endothelial ◽  
Blastocyst Formation ◽  
Parthenogenetic Activation ◽  
Maturation Rate ◽  
Endothelial Growth Factor

The aim of the present study was to investigate the effect of vascular endothelial growth factor (VEGF) on the meiotic and developmental competence of porcine oocytes from small follicles (SF; 0.5–3 mm diameter). When cumulus–oocyte complexes (COCs) from medium-sized follicles (MF; 3–6 mm diameter) and SF were cultured for IVM, the maturation rates were significantly higher for oocytes from MF than SF. Concentrations of VEGF in the medium were significantly higher for COCs cultured from MF than SF. When COCs from SF were exposed to 200 ng mL–1 VEGF during the first 20 h of IVM, the maturation rate improved significantly and was similar to that of oocytes derived from MF. The fertilisability of oocytes was also significantly higher than that of VEGF-free SF controls. Following parthenogenetic activation, the blastocyst formation rate improved significantly when SF COC culture was supplemented with 200 ng mL–1 VEGF, with the rate similar to that of oocytes from MF. The results of the present study indicate that VEGF markedly improves the meiotic and developmental competence of oocytes derived from SF, especially at a concentration of 200 ng mL–1 during the first 20 h of IVM.

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