scholarly journals Epitope Analysis of Staphylococcal Enterotoxin A Using Different Synthetic Peptides.

1998 ◽  
Vol 60 (9) ◽  
pp. 993-996 ◽  
Author(s):  
Dong-Liang HU ◽  
Ayako IMAI ◽  
Katsuhiko ONO ◽  
Sanae SASAKI ◽  
Akio NAKANE ◽  
...  
Keyword(s):  
Synthetic Peptides ◽  
Staphylococcal Enterotoxin ◽  
Staphylococcal Enterotoxin A ◽  
Epitope Analysis

Inhibition of emetic and superantigenic activities of staphylococcal enterotoxin A by synthetic peptides

Peptides ◽  
2012 ◽  
Vol 38 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Edward K. Maina ◽  
Dong-Liang Hu ◽  
Krisana Asano ◽  
Akio Nakane
Keyword(s):  
Synthetic Peptides ◽  
Staphylococcal Enterotoxin ◽  
Staphylococcal Enterotoxin A

Immunofluorescence Assay Using Monoclonal and Polyclonal Antibodies for Detection of Staphylococcal Enterotoxins A in Milk

2019 ◽  
Vol 13 (1) ◽  
pp. 137-145 ◽  
Author(s):  
Tzonka Godjevargova ◽  
Zlatina Becheva ◽  
Yavor Ivanov ◽  
Andrey Tchorbanov
Keyword(s):  
Monoclonal Antibody ◽  
Magnetic Nanoparticles ◽  
Polyclonal Antibody ◽  
Polyclonal Antibodies ◽  
Food Poisoning ◽  
Staphylococcal Enterotoxin ◽  
Staphylococcal Enterotoxin A ◽  
Fluorescence Immunoassay ◽  
Magnetic Separator ◽  
Milk Samples

Objectives: Staphylococcus aureus is a Gram-positive microorganism. S. aureus can grow in various foods and cause food poisoning by secreting enterotoxins. The most common enterotoxins involved in food poisoning are staphylococcal enterotoxin A and staphylococcal enterotoxin B, but Staphylococcal Enterotoxin A (SEA) is predominant. The main types of food contaminated with SEs are meat and meat products, poultry and eggs, milk and dairy products. The aim of this study was to develop a rapid and sensitive fluorescence immunoassay for detection of staphylococcal enterotoxin A in milk. Methods: Monoclonal and polyclonal antibodies for SEA were produced and characterized. Competitive fluorescence immunoassay based on Magnetic Nanoparticles (MNPs) was performed and optimized. MNPs were used as a solid carrier of the antibodies. The first step of the assay was immunoreaction between the immobilized antibody onto MNPs and SEA in milk sample. Then the fluorescein-SEA conjugate was added to the sample. Thus, competitive immunoreaction between MNP-mAb/MNP-pAb with SEA and SEA-FITC was performed. These immuno-complexes were separated by a magnetic separator and the obtained supernatants were analyzed. The fluorescent signal from the excess of conjugated SEA was proportional to the SEA contained in the milk. The assay duration was only 30 min. Results: The fluorescence immunoassays performed with polyclonal antibody had linear ranges from 5 pg/mL to 100 ng/mL SEA in a buffer, and from 50 pg/mL to 50 ng/mL SEA in spiked milk samples. While the same assays performed with monoclonal antibody had linear ranges from 1 pg/mL to 20 ng/mL SEA in buffer, and from 10 pg/mL to 10 ng/mL SEA in spiked milk samples. The detection limits of the developed immunoassays performed in milk were: 48 pg/mL with polyclonal antibody and 9 pg/mL with monoclonal antibody. Conclusion: A rapid and sensitive fluorescence immunoassay based on magnetic nanoparticles with a polyclonal and monoclonal antibody for determination of staphylococcal enterotoxin A in milk was developed.

Epitope Analysis of Antibodies in Japanese to Human Cytomegalovirus Phosphoprotein 150 with Synthetic Peptides

2002 ◽  
Vol 66 (11) ◽  
pp. 2402-2405 ◽  
Author(s):  
Isao TAKAHASHI ◽  
Sayoko SUGIURA ◽  
Hirotoshi OHTA ◽  
Kazuo OZAWA ◽  
Tadashi KAMIYA
Keyword(s):  
Human Cytomegalovirus ◽  
Synthetic Peptides ◽  
Epitope Analysis

scholarly journals Mass Outbreak of Food Poisoning Disease Caused by Small Amounts of Staphylococcal Enterotoxins A and H

2005 ◽  
Vol 71 (5) ◽  
pp. 2793-2795 ◽  
Author(s):  
Tetsuya Ikeda ◽  
Naoto Tamate ◽  
Keiji Yamaguchi ◽  
Sou-ichi Makino
Keyword(s):  
Food Poisoning ◽  
Staphylococcal Enterotoxin ◽  
Raw Material ◽  
Staphylococcal Enterotoxin A ◽  
Staphylococcal Enterotoxins ◽  
Reconstituted Milk

ABSTRACT It was believed that food poisoning in Osaka in 2000 was due to small amounts of staphylococcal enterotoxin A (SEA) in reconstituted milk. Results of this study clearly indicate that SEH was also present in the raw material of reconstituted milk, indicating that the food poisoning was caused by multiple staphylococcal enterotoxins.

scholarly journals Production of Staphylococcal Enterotoxin A in Blue, Brick, Mozzarella, and Swiss Cheeses

1973 ◽  
Vol 56 (4) ◽  
pp. 429-435 ◽  
Author(s):  
S.R. Tatini ◽  
W.D. Wesala ◽  
J.J. Jezeski ◽  
H.A. Morris
Keyword(s):  
Staphylococcal Enterotoxin ◽  
Staphylococcal Enterotoxin A
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