scholarly journals Identification of Bovine Serum Transferrin Phenotypes by Polyacrylamide Gel Isoelectric Focusing (PAGIEF).

1994 ◽  
Vol 56 (2) ◽  
pp. 421-423 ◽  
Author(s):  
Kiyotaka WATANABE ◽  
Yukihisa SAKASHITA ◽  
Kouichi ORINO ◽  
Shinji YAMAMOTO
Keyword(s):  
Isoelectric Focusing ◽  
Bovine Serum ◽  
Polyacrylamide Gel ◽  
Serum Transferrin ◽  
Gel Isoelectric Focusing

Detection of the Genetic Polymorphism of Human C2 (Native Protein and C2a Fragment) by Immunoblotting after Polyacrylamide Gel Isoelectric Focusing

Complement ◽  
1985 ◽  
Vol 2 (4) ◽  
pp. 185-192 ◽  
Author(s):  
B. Uring-Lambert ◽  
S. Gas ◽  
J. Goetz ◽  
G. Mauff ◽  
S.F. Goldmann ◽  
...  
Keyword(s):  
Genetic Polymorphism ◽  
Isoelectric Focusing ◽  
Polyacrylamide Gel ◽  
Native Protein ◽  
Gel Isoelectric Focusing

Salivary deoxyribounuclease I polymorphism separated by polyacrylamide gel-isoelectric focusing and detected by the dried agarose film overlay method

1993 ◽  
Vol 14 (1) ◽  
pp. 1042-1044 ◽  
Author(s):  
Etsuko Tenjo ◽  
Kazumi Sawazaki ◽  
Toshihiro Yasuda ◽  
Daita Nadano ◽  
Haruo Takeshita ◽  
...  
Keyword(s):  
Isoelectric Focusing ◽  
Polyacrylamide Gel ◽  
Overlay Method ◽  
Gel Isoelectric Focusing

Fish Species Identification by Isoelectric Focusing: Sarcoplasmic Protein Polymorphism in Monkfish (Lophius americanus)

1981 ◽  
Vol 64 (1) ◽  
pp. 32-37
Author(s):  
Ronald C Lundstrom
Keyword(s):  
Species Identification ◽  
Isoelectric Focusing ◽  
Fish Species ◽  
Protein Pattern ◽  
Polyacrylamide Gel ◽  
Protein Patterns ◽  
Fish Species Identification ◽  
Sarcoplasmic Protein ◽  
Gel Isoelectric Focusing ◽  
Agarose Ief

Abstract Monkfish (Lophius americanus) sarcoplasmic protein patterns were found to be polymorphic with respect to separations using isoelectric focusing. Reproducible protein pattern variations were not detected using cellulose acetate or polyacrylamide gel disc electrophoresis. Monkfish sarcoplasmic proteins separated on pH 3.5-9.5 Ampholine PAGplates or on pH 2.5-9.0 agarose IEF gels yielded similar patterns showing 3 distinct, reproducible variations. On close examination, the pH 3.5-9.5 Ampholine PAGplate patterns could be further subdivided into 3 additional variations. A high resolution pH 3.5-5.0 agarose IEF gel was able to resolve a total of 10 different monkfish pattern variations in a sample of 24 individuals. A model was proposed suggesting the existence of 16 distinct variations in the monkfish sarcoplasmic protein pattern, based on the various combinations of 4 protein bands. In identifying samples of monkfish meat, it is necessary to compare the unknown pattern with the possible variant patterns to effect a reliable identification. On recommendation by the Associate Referee, the method for fish species identification based on polyacrylamide gel isoelectric focusing, 18.A01-18.A04, has been adopted official final action with no restrictions as to the species that may be identified.

Sign in / Sign up

Export Citation Format

Share Document