scholarly journals Nucleotide Sequence of the Glycoprotein C(gC) Homologous Gene of Marek's Disease Virus(MDV) Serotype 2 and Comparison of gC Homologous Genes among Three Serotypes of MDV.

1993 ◽  
Vol 55 (6) ◽  
pp. 985-990 ◽  
Author(s):  
Takehisa KITAZAWA ◽  
Mitsuru ONO ◽  
Ken MAEDA ◽  
Yasushi KAWAGUCHI ◽  
Naohiro KAMIYA ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Disease Virus ◽  
Marek's Disease Virus ◽  
Marek's Disease ◽  
Homologous Gene ◽  
Marek’S Disease Virus ◽  
Marek’S Disease ◽  
Glycoprotein C ◽  
Homologous Genes

scholarly journals Characterization of Marek's Disease Virus Serotype 1 (MDV-1) Deletion Mutants That Lack UL46 to UL49 Genes: MDV-1 UL49, Encoding VP22, Is Indispensable for Virus Growth

2002 ◽  
Vol 76 (4) ◽  
pp. 1959-1970 ◽  
Author(s):  
Fabien Dorange ◽  
B. Karsten Tischer ◽  
Jean-François Vautherot ◽  
Nikolaus Osterrieder
Keyword(s):  
Amino Acids ◽  
Disease Virus ◽  
Marek's Disease Virus ◽  
Marek's Disease ◽  
Homologous Gene ◽  
Marek’S Disease Virus ◽  
Marek’S Disease ◽  
Virus Growth ◽  
Virus Serotype ◽  
Serotype 1

ABSTRACT Experiments were conducted to investigate the roles of Marek's disease virus serotype 1 (MDV-1) major tegument proteins VP11/12, VP13/14, VP16, and VP22 in viral growth in cultured cells. Based on a bacterial artificial chromosome clone of MDV-1 (BAC20), mutant viruses were constructed in which the MDV-1 homologs of UL46, UL47, UL48, or UL49 were deleted alone and in various combinations. It could be demonstrated that the UL46, UL47, and UL48 genes are dispensable for MDV-1 growth in chicken embryonic skin and quail muscle QM7 cells, although the generated virus mutants exhibited reduced plaque sizes in all cell types investigated. In contrast, a UL49-negative MDV-1 (20Δ49) and a UL48-UL49 (20Δ48-49) doubly negative mutant were not able to produce MDV-1-specific plaques on either cell type. It was confirmed that this growth restriction is dependent on the absence of VP22 expression, because growth of these mutant viruses could be partially restored on cells that were cotransfected with a UL49 expression plasmid. In addition, we were able to demonstrate that cell-to-cell spread of MDV-1 conferred by VP22 is dependent on the expression of amino acids 37 to 187 of MDV-1 VP22, because expression plasmids containing MDV-1 UL49 mutant genes with deletions of amino acids 1 to 37 or 188 to 250 were still able to restore partial growth of the 20Δ49 and 20Δ48-49 viruses. These results demonstrate for the first time that an alphaherpesvirus UL49-homologous gene is essential for virus growth in cell culture.

Molecular Analysis of the Glycoprotein C-Negative Phenotype of Attenuated Marek's Disease Virus

Virology ◽  
1994 ◽  
Vol 199 (2) ◽  
pp. 393-402 ◽  
Author(s):  
Melinda R. Wilson ◽  
Ronald A. Southwick ◽  
James T. Pulaski ◽  
Virginia L. Tieber ◽  
Yu Hong ◽  
...  
Keyword(s):  
Disease Virus ◽  
Molecular Analysis ◽  
Marek's Disease Virus ◽  
Marek's Disease ◽  
Marek’S Disease Virus ◽  
Marek’S Disease ◽  
Glycoprotein C ◽  
Negative Phenotype

Isolation and identification of Marek’s disease virus (MDV) from feather follicle epithelium and internal organs of diseased chickens in Dakahlia Governorate, Egypt

2019 ◽  
Vol 20 (2) ◽  
pp. 6-11
Author(s):  
Aly El-Kenawy ◽  
Mohamed El-Tholoth ◽  
Emad A
Keyword(s):  
Real Time ◽  
Disease Virus ◽  
Real Time Pcr ◽  
Marek's Disease Virus ◽  
Marek's Disease ◽  
Marek’S Disease Virus ◽  
Marek’S Disease ◽  
Field Samples ◽  
Feather Follicle ◽  
Positive Results

In the present study, a total of 16 samples including feather follicle epithelium, ovary, spleen and kidney (4 samples for each organ) were collected from diseased chicken flocks suspected to be infected with Marek’s disease virus (MDV) at Dakahlia Governorate, Egypt during the period from October 2016 to October 2017. Each sample was pooled randomly from three to five birds (90 to 360 days old). The isolation of the suspected virus from the collected samples was carried out via chorioallantoic membranes (CAMs) of 12 days old embryonated chicken eggs (ECEs). Three egg passages were carried out for each sample. Hyperimmune serum was prepared against standard MDV. MDV in both field and egg passaged samples (after 3rd passage) was identified by agar gel precipitation test (AGPT) and indirect fluorescence antibody test (IFAT). Molecular identification of virus was carried out by conventional polymerase chain reaction (PCR) and real- time PCR in four selected samples. The results revealed that 14 samples (87.5%) including 4 (100%) samples from feather follicle epithelium, ovary and kidney and 2 (50%) samples from spleen, showed positive results in virus isolation after 3rd passage. The positive results percentage by AGPT for field samples were 50% (8 out of 16 samples), while after the 3rd passage in ECEs were 37.5% (6 out of 16 samples) and the positive results percentage by IFAT for field samples were 62.5% (10 out of 16 samples), while after the 3rd passage in ECEs were 81.25 % (13 out of 16 samples). Viral nucleic acid was detected in all selected samples by conventional and real- time PCR. The results indicate that feather follicle epithelium is the best organ for MDV detection. IFAT is superior over AGPT in virus detection. Conventional and real - time PCR could be efficiently used for molecular detection of the virus.

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