scholarly journals Serum Hemolytic Activity of Babesia gibsoni-Infected Dogs: The Difference in the Activity between Self and Nonself Red Blood Cells.

1993 ◽  
Vol 55 (2) ◽  
pp. 203-206 ◽  
Author(s):  
Takafumi ONISHI ◽  
Sayoko SUZUKI ◽  
Makio HORIE ◽  
Masatoshi HASHIMOTO ◽  
Taketsugu KAJIKAWA ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Hemolytic Activity ◽  
Blood Cells ◽  
Babesia Gibsoni ◽  
The Difference

Permeation of the luminal capillary glycocalyx is determined by hyaluronan

1999 ◽  
Vol 277 (2) ◽  
pp. H508-H514 ◽  
Author(s):  
Charmaine B. S. Henry ◽  
Brian R. Duling
Keyword(s):  
Blood Cell ◽  
Red Blood Cells ◽  
Red Blood Cell ◽  
Blood Cells ◽  
Enzyme Treatment ◽  
Apical Surface ◽  
Bright Field ◽  
Endothelial Surface ◽  
The Difference

The endothelial cell glycocalyx influences blood flow and presents a selective barrier to movement of macromolecules from plasma to the endothelial surface. In the hamster cremaster microcirculation, FITC-labeled Dextran 70 and larger molecules are excluded from a region extending almost 0.5 μm from the endothelial surface into the lumen. Red blood cells under normal flow conditions are excluded from a region extending even farther into the lumen. Examination of cultured endothelial cells has shown that the glycocalyx contains hyaluronan, a glycosaminoglycan which is known to create matrices with molecular sieving properties. To test the hypothesis that hyaluronan might be involved in establishing the permeation properties of the apical surface glycocalyx in vivo, hamster microvessels in the cremaster muscle were visualized using video microscopy. After infusion of one of several FITC-dextrans (70, 145, 580, and 2,000 kDa) via a femoral cannula, microvessels were observed with bright-field and fluorescence microscopy to obtain estimates of the anatomic diameters and the widths of fluorescent dextran columns and of red blood cell columns (means ± SE). The widths of the red blood cell and dextran exclusion zones were calculated as one-half the difference between the bright-field anatomic diameter and the width of the red blood cell column or dextran column. After 1 h of treatment with active Streptomyces hyaluronidase, there was a significant increase in access of 70- and 145-kDa FITC-dextrans to the space bounded by the apical glycocalyx, but no increase in access of the red blood cells or in the anatomic diameter in capillaries, arterioles, and venules. Hyaluronidase had no effect on access of FITC-Dextrans 580 and 2,000. Infusion of a mixture of hyaluronan and chondroitin sulfate after enzyme treatment reconstituted the glycocalyx, although treatment with either molecule separately had no effect. These results suggest that cell surface hyaluronan plays a role in regulating or establishing permeation of the apical glycocalyx to macromolecules. This finding and our prior observations suggest that hyaluronan and other glycoconjugates are required for assembly of the matrix on the endothelial surface. We hypothesize that hyaluronidase creates a more open matrix, enabling smaller dextran molecules to penetrate deeper into the glycocalyx.

Nanotoxicity of Silver Nanoparticles to Red Blood Cells: Size Dependent Adsorption, Uptake, and Hemolytic Activity

2015 ◽  
Vol 28 (3) ◽  
pp. 501-509 ◽  
Author(s):  
Li Qiang Chen ◽  
Li Fang ◽  
Jian Ling ◽  
Cheng Zhi Ding ◽  
Bin Kang ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Red Blood Cells ◽  
Hemolytic Activity ◽  
Blood Cells ◽  
Size Dependent

Cell volume and metabolic dependence of NEM-activated K+-Cl- flux in human red blood cells

1985 ◽  
Vol 249 (1) ◽  
pp. C124-C128 ◽  
Author(s):  
P. K. Lauf ◽  
C. M. Perkins ◽  
N. C. Adragna
Keyword(s):  
Red Blood Cells ◽  
Cell Volume ◽  
Blood Cells ◽  
Time Course ◽  
Transport Activity ◽  
Human Red Blood Cells ◽  
Atp Breakdown ◽  
The Difference ◽  
Metabolic Dependence ◽  
K Transport

The effects of incubation in anisosmotic media and of metabolic depletion on ouabain-resistant (OR) Cl--dependent K+ influxes stimulated by N-ethylmaleimide (NEM) were studied in human red blood cells using Rb+ as K+ analogue. The NEM-stimulated but not the basal Rb+-Cl- influx measured in phosphate-buffered anisosmotic media was found to be cell volume dependent. When cellular ATP, [ATP]c, was lowered to less than 0.10 of its initial level by exposure to nonmetabolizable 2-deoxy-D-glucose, the NEM-stimulated but not the basal Cl--dependent Rb+ influxes were abolished. Metabolically depleted red blood cells subsequently repleted by incubation in glucose plus inosine regained the NEM-inducible Rb+ (K+) transport activity. The difference in the time course of ATP breakdown and Rb+ influx inhibition suggests that energization of the NEM-stimulated Rb+ flux by metabolism may involve factors additional to ATP.

In vitro hemolytic activity ofPlasmodium berghei on red blood cells

1980 ◽  
Vol 2 (2) ◽  
pp. 129-134 ◽  
Author(s):  
Sudhir Gupta ◽  
K. C. Saxena
Keyword(s):  
Red Blood Cells ◽  
Hemolytic Activity ◽  
Blood Cells

scholarly journals Biconcave shape of human red-blood-cell ghosts relies on density differences between the rim and dimple of the ghost's plasma membrane

2016 ◽  
Vol 113 (51) ◽  
pp. 14847-14851 ◽  
Author(s):  
Joseph F. Hoffman
Keyword(s):  
Blood Cell ◽  
Red Blood Cells ◽  
Red Blood Cell ◽  
Blood Cells ◽  
Theoretical Work ◽  
Centrifugal Field ◽  
Hypotonic Hemolysis ◽  
Osmotic Solution ◽  
Dense Band ◽  
The Difference

The shape of the human red blood cell is known to be a biconcave disk. It is evident from a variety of theoretical work that known physical properties of the membrane, such as its bending energy and elasticity, can explain the red-blood-cell biconcave shape as well as other shapes that red blood cells assume. But these analyses do not provide information on the underlying molecular causes. This paper describes experiments that attempt to identify some of the underlying determinates of cell shape. To this end, red-blood-cell ghosts were made by hypotonic hemolysis and then reconstituted such that they were smooth spheres in hypo-osmotic solutions and smooth biconcave discs in iso-osmotic solutions. The spherical ghosts were centrifuged onto a coated coverslip upon which they adhered. When the attached spheres were changed to biconcave discs by flushing with an iso-osmotic solution, the ghosts were observed to be mainly oriented in aflatalignment on the coverslip. This was interpreted to mean that, during centrifugation, the spherical ghosts were oriented by a dense band in its equatorial plane, parallel to the centrifugal field. This appears to be evidence that the difference in the densities between the rim and the dimple regions of red blood cells and their ghosts may be responsible for their biconcave shape.

THE PRODUCTION OF PURPURA BY DERIVATIVES OF PNEUMOCOCCUS

1926 ◽  
Vol 43 (1) ◽  
pp. 111-106
Author(s):  
Hobart A. Reimann ◽  
Louis A. Julianelle
Keyword(s):  
Red Blood Cells ◽  
Hemolytic Activity ◽  
Blood Cells ◽  
Blood Platelets ◽  
White Blood Cells ◽  
Red Cells ◽  
Severe Anemia ◽  
Marked Diminution ◽  
Derivatives Of

A study has been made of the variation in number of the blood platelets, and the red and white blood cells of white mice injected with pneumococcus extract. The blood platelets were greatly diminished after the injection, the greatest decrease usually occurring after 24 hours. Purpuric lesions usually developed when the number of blood platelets became less than 500,000 per c.mm. Regeneration of the platelets was accomplished by the 4th to the 9th day but there was an overregeneration and the return to normal did not take place until 2 weeks had elapsed. The red cells were also greatly reduced in number, but the rate of their destruction and regeneration was somewhat slower than that of the platelets. The leucocytes were slightly if at all influenced by the pneumococcus extract. Pneumococcus extracts were shown to be thrombolytic and hemolytic. Heat destroyed the activity of both the lysins in vitro. Heated extract produced purpura in mice but did not cause a severe anemia. Extracts adsorbed with either blood platelets or red blood cells showed a marked diminution in their thrombolytic and hemolytic activity in vitro. Such extracts, however, produced purpura as well as severe anemia and thrombopenia in mice.

scholarly journals Pengaruh Konsentrasi IgY Induk yang Berbeda Terhadap Gambaran Darah Anak Ayam Sentul

2019 ◽  
Vol 6 (2) ◽  
pp. 273
Author(s):  
Melly Pratiwi Setyawati ◽  
Niken Ulupi ◽  
Sri Murtini ◽  
Cece Sumantri
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
White Blood Cells ◽  
T Test ◽  
Blood Picture ◽  
Test Program ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
The Difference

ABSTRAKPenelitian ini bertujuan untuk menganalisis gambaran sel darah putih dan sel darah merah sebagai indikator ketahanan tubuh pada anak ayam sentul. Penelitian ini menggunakan 20 ekor ayam betina dan 20 ekor ayam jantan yang dikawinkan, masing masing terdiri dari 10 ekor ayam dengan konsentrasi IgY tinggi dan 10 ekor ayam dengan konsentrasi IgY rendah. Berdasarkan hasil perkawinan tersebut diperolah 34 ekor anak ayam yang terdiri dari 17 ekor anak ayam yang berasaldari induk dengan konsentrasi IgY rendah. Parameter yang diamati meliputi gambaran sel darah merah (eritrosit, hematocrit dan indeks eritrosit) dan gambaran sel darah putih (leukosit dan diferensiasinya). Penelitian ini menggunakan rancangan acak lengkap (RAL) dengan 2 perlakuan, semua perlakuan diulang 10 kali. Data yang diperoleh dianalisis menggunakan t-test program SAS.  Hasil penelitian ini menunjukan bahwa perbedaan konsentrasi IgY pada induk tidak mempengaruhi sel darah merah dan sel darah putih. Namun terdapat kecendrungan nilai limfosit yang rendah dan H/L yang tinggi pada anak ayam yang berasal dari induk dengan konsentrasi IgY rendah. Hasil ini mengindikasi bahwa anak ayam yang berasal dari induk dengan konsentrasi IgY rendah lebih rentan terhadap cekaman panas. Kata Kunci: ayam sentul, IgY,  gambaran darah, ketahanan tubuhABSTRACTThis study was to aim the description of white blood cells and red blood cells as indicators of immunity in Sentul chicks. This study used 20 hen chickens and 20 cock-chicken mated, consisting of 10 chickens with high IgY concentration and 10 chickens with low IgY concentrations. Based on the results of mating, 34 chicks were obtained consisting of 17 chicks that were from the hen with low IgY concentration. The parameters observed included the description of red blood cells (erythrocytes, hematocrit and erythrocyte index) and the description of white blood cells (leukocytes and their differentiation). The experiment was conducted as a completely randomized design with 2 treatments, all treatments were repeated 10 times. The data obtained were analyzed using the SAS program t-test. The results of this study indicate that the difference in IgY concentration in the hen did not effect on red blood cells and white blood cells. However, there is a tendency for low lymphocyte values and high H/ L in chicks originating from hens with low IgY concentrations. These results indicate that chicks from hens with low IgY concentrations are more susceptible to heat stress.Keyword: blood picture, IgY, immunity, sentul chicken

scholarly journals Interaction of HK and LK Goat Red Blood Cells with Ouabain

1974 ◽  
Vol 64 (5) ◽  
pp. 536-550 ◽  
Author(s):  
John R. Sachs ◽  
Philip B. Dunham ◽  
Donna L. Kropp ◽  
J. Clive Ellory ◽  
Joseph F. Hoffman
Keyword(s):  
Red Blood Cells ◽  
Binding Sites ◽  
Blood Cells ◽  
Red Cells ◽  
Ouabain Binding ◽  
High Potassium ◽  
Considerable Heterogeneity ◽  
Low Potassium ◽  
The Difference ◽  
Calculated Number

The characteristics of the interaction of Na-K pumps of high potassium (HK) and low potassium (LK) goat red blood cells with ouabain have been determined. The rate of inhibition by ouabain of the pump of HK cells is greater than the rate of inhibition of the pumps of LK cells. Treatment of LK cells with an antibody (anti-L) raised in HK sheep by injecting LK sheep red cells increases the rate of inhibition of the LK pumps by ouabain to that characteristic of HK pumps; reduction of intracellular K (Kc) in LK cells increases the rate at which ouabain inhibits their pumps and exposure of these low Kc cells to anti-L does not affect the rate of inhibition. There is considerable heterogeneity in the pumps of both HK and LK cells in the rate at which they interact with ouabain or the rate at which they pump or both. LK pumps which are sensitive to stimulation by anti-L bind ouabain less rapidly than the remainder of the LK pumps and exposure to antibody increases the rate at which ouabain binds to the sensitive pumps; the difference between the two types of pumps disappears if intracellular K is very low. The calculated number of ouabain molecules bound at 100% inhibition of the pump is about the same for HK and LK cells. Although exposure to anti-L increases the apparent number of ouabain binding sites in LK cells at normal Kc, it does not alter the apparent number of sites in LK cells when Kc has been reduced.

scholarly journals Mesoporous Silica Nanoparticles for Reducing Hemolytic Activity Towards Mammalian Red Blood Cells

Small ◽  
2009 ◽  
Vol 5 (1) ◽  
pp. 57-62 ◽  
Author(s):  
Igor I. Slowing ◽  
Chia-Wen Wu ◽  
Juan L. Vivero-Escoto ◽  
Victor S.-Y. Lin
Keyword(s):  
Mesoporous Silica ◽  
Red Blood Cells ◽  
Silica Nanoparticles ◽  
Hemolytic Activity ◽  
Blood Cells ◽  
Mesoporous Silica Nanoparticles
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