scholarly journals In Vitro Fertilization of Follicular Oocytes from the Swamp Buffalo (Bubalus bubalis) and Kedah-Kelantan Cattle (Bos indicus) in Malaysia.

1992 ◽  
Vol 54 (4) ◽  
pp. 799-801 ◽  
Author(s):  
Yoshiyuki TAKAHASHI ◽  
Mohammad NIHAYAH ◽  
Mohamed R. JAINUDEEN ◽  
Mitsugu HISHINUMA ◽  
Yukio KANAI ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Bos Indicus ◽  
Bubalus Bubalis ◽  
Vitro Fertilization ◽  
Swamp Buffalo

Selection of Reference miRNAs for Relative Quantification in Buffalo (Bubalus bubalis) Blastocysts Produced by Hand-Made Cloning and In Vitro Fertilization

2019 ◽  
Vol 21 (4) ◽  
pp. 200-209 ◽  
Author(s):  
Swati Viviyan Lagah ◽  
Tanushri Jerath Sood ◽  
Prabhat Palta ◽  
Manishi Mukesh ◽  
Manmohan Singh Chauhan ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Bubalus Bubalis ◽  
Relative Quantification ◽  
Vitro Fertilization ◽  
Selection Of ◽  
Reference Mirnas

RNA sequencing and transcriptome analysis of buffalo ( Bubalus bubalis ) blastocysts produced by somatic cell nuclear transfer and in vitro fertilization

2019 ◽  
Vol 86 (9) ◽  
pp. 1149-1167 ◽  
Author(s):  
Tanushri Jerath Sood ◽  
Swati Viviyan Lagah ◽  
Manishi Mukesh ◽  
Suresh Kumar Singla ◽  
Manmohan Singh Chauhan ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Somatic Cell ◽  
Rna Sequencing ◽  
Transcriptome Analysis ◽  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer ◽  
Bubalus Bubalis ◽  
Vitro Fertilization

scholarly journals Production by in vitro fertilization of vietnam swamp buffalo embryos

2014 ◽  
Vol 27 (3) ◽  
Author(s):  
Le Van Ty ◽  
Hoang Nghia Son ◽  
Nguyen Mong Hung
Keyword(s):  
In Vitro Fertilization ◽  
Vitro Fertilization ◽  
Swamp Buffalo

89 OPEN PULLED STRAW VITRIFICATION FOR IN VITRO-PRODUCED BUFFALO (BUBALUS BUBALIS) EMBRYOS

2006 ◽  
Vol 18 (2) ◽  
pp. 153 ◽  
Author(s):  
A. De Rosa ◽  
R. Di Palo ◽  
L. Attanasio ◽  
E. Monaco ◽  
G. Campanile ◽  
...  
Keyword(s):  
Survival Rate ◽  
In Vitro Fertilization ◽  
Developmental Stages ◽  
Sucrose Solution ◽  
Bubalus Bubalis ◽  
Embryo Survival ◽  
Vitro Fertilization ◽  
Arcsine Transformation ◽  
Further Development

The aim of this study was to compare the efficiency of different combinations of cryoprotectants for vitrification of IVP buffalo (Bubalus bubalis) embryos at different developmental stages by the open pulled straw (OPS) method. In method A, we evaluated the vitrification and warming solutions previously used to vitrify buffalo embryos in French straws (Gasparrini et al. 2001 Theriogenology 55, 307). Embryos were equilibrated in 1.4 M glycerol for 5 min before being placed into 1.4 M glycerol + 3.6 M ethylene glycol (EG) for 5 min. Then, embryos were transferred into 3.4 M glycerol + 4.6 M EG for 25 s and loaded into the OPSs. For warming, OPSs were briefly immersed in a 0.5 M sucrose solution; the embryos were exposed to 0.25 M sucrose for 5 min before transfer to SOF medium for culture. In Method B, we examined the vitrification and warming solutions previously used for OPS vitrification of cattle embryos (Vajta et al. 1998 Mol. Reprod. Dev. 51, 53-58). Buffalo embryos were equilibrated in 7.5% EG + 7.5% dimethyl sulfoxide (DMSO) for 3 min before transfer into 16.5% EG + 16.5% DMSO and 0.5 M sucrose. After 25 s, they were loaded into the OPSs. For warming, embryos were recovered in a 0.25 M sucrose solution and transferred into a 0.15 M sucrose solution for 5 min before being placed in SOF medium. A total of 293 IVP buffalo embryos (eight replicates) were vitrified at Day 7 of culture (Day 0 = in vitro fertilization). Embryos were vitrified at the following developmental stages: early blastocyst (eBL, n = 26 and 34 with methods A and B, respectively), blastocyst (Bl, n = 31 and 35 for Methods A and B, respectively), expanded blastocyst (XBl, n = 29 and 38 for Methods A and B, respectively), and hatched blastocyst (HBl, n = 46 and 54 for Methods A and B, respectively). Embryo survival rate was determined as the percentage of vitrified-warmed embryos undergoing further development during a 24-h in vitro culture period. Differences between methods were analyzed by ANOVA following arcsine transformation of data. The overall embryo survival rate recorded at 24 h was not significantly different between Methods A and B (70% vs. 62%, respectively). Specifically, no differences were observed in embryos vitrified at the eBL (70% vs. 73%, A and B, respectively), Bl (69% vs. 70%, A and B, respectively), and HBl (46% vs. 36%, A and B, respectively) stages. In contrast, a significantly higher survival rate was recorded for XBl-stage embryos vitrified-warmed by Method A as compared to Method B (90% vs. 53%, respectively; P < 0.01). In Method A, survival rate of XBl was significantly higher than that of HBl (P < 0.05), but it was not different from that of eBl and Bl. Within Method B, the survival efficiency was similar for eBL, BL, and XBl, whereas survival rate of HBl was significantly lower (P < 0.05). In conclusion, although overall embryo survival in vitro was similar between methods, the combination of cryoprotectants used in Method A seemed more suitable for vitrification of IVP buffalo embryos at the XBl stage.

262 ASSESSMENT OF HSP70-1 TRANSCRIPTION LEVELS IN IMMATURE OOCYTES FROM BOS TAURUS AND BOS INDICUS COWS RAISED IN A TROPICAL CLIMATE

2007 ◽  
Vol 19 (1) ◽  
pp. 247
Author(s):  
L. S. A. Camargo ◽  
J. H. M Viana ◽  
R.V. Serapião ◽  
M. F. M. Guimarães ◽  
W. F. Sá ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Real Time ◽  
Real Time Pcr ◽  
Bos Taurus ◽  
Rna Extraction ◽  
Bos Indicus ◽  
Tropical Climate ◽  
Relative Quantification ◽  
Vitro Fertilization

Heat stress is one of the main causes of low conception rate in Bos taurus cows in a tropical climate. On the other hand, in this environment, oocytes from Bos indicus show greater developmental capacity after in vitro fertilization than those from Bos taurus, suggesting an adaptation to the hot climate. Heat shock proteins (HSP) are chaperones that promote protection against heat damage, and their transcription is associated to stress. The aim of this study was to evaluate the expression of HSP70-1 gene (Genbank NM174550), a member of HSP family, in oocytes from Bos taurus (Holstein) and Bos indicus (Gyr) cows raised in the tropical climate located at 21�35′′S latitude, 43�51′′W longitude, and 435 m altitude. Cumulus–oocyte complexes were recovered by oocyte pickup from mature non-lactating Holstein (n = 4) and Gyr (n = 4) donor cows during the hot season. Cumulus cells of viable oocytes were removed by vortexing in TALP-HEPES plus BSA, and pools (3 for each breed) with 12 immature oocytes were rapidly frozen in liquid nitrogen and subsequently thawed for RNA extraction. Total RNA extraction was performed using Rneasy� Micro kit (Qiagen, Valencia, CA, USA), and first strands were synthesized using SuperscriptTM III First Strand Synthesis kit (Invitrogen, Chicago, IL, USA). Relative quantification was performed in duplicate using real-time PCR (ABI Prism� 7000; Applied Biosystems, Foster City, CA, USA); reactions consisted of a mixture of iTaqTM SYBR� Green Supermix with ROX (Bio-Rad, Waltham, MA, USA) and cDNA equivalent to 1.2 oocytes and gene specific primers. Expression of the GAPDH gene was used as endogenous reference. Calculations of relative quantification were performed by the comparative Ct method, using the lowest value found in Bos indicus oocytes as calibrator; values (mean � SE) are shown as n-fold difference relative to the calibrator. Statistical comparison between breeds was performed by analysis of variance. Oocytes from Holstein cows showed a higher level (P &lt; 0.05) of HSP70-1 expression (1.82 � 0.22) than oocytes recovered from Gyr cows (1.12 � 0.11). Previous study reported that oocytes from Gyr cows in a tropical climate showed a higher blastocyst rate after in vitro fertilization than Holstein oocytes (Camargo et al. 2006 Reprod. Fertil. Dev. 18, 243 abst). The lower level of HSP70-1 in Gyr oocytes suggests that they were less subject to stress than the Holstein ones, which may reflect their capacity to develop after fertilization. This effect may be, at least in part, due to the ability of Bos indicus cows to regulate body temperature in a hot environment, causing less stress on oocytes. Financial support was provided by FAPEMIG, MG, Brazil, and CNPq, DF, Brazil. Thanks to Agrogen�tica, Vi�osa, Brazil, for the real-time PCR machine.

Sign in / Sign up

Export Citation Format

Share Document